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The sequence of a gene encoding convicilin from pea
The sequence of a gene encoding convicilin from pea

... pea seeds, in addition to legumin and vicilin [1]. It can be purified from both legumin and vicilin, and it consists solely of polypeptides of Mr approx. 71 000. It does not thus contain polypeptides found in either of the two major storage proteins [2]. On the other hand, convicilin is antigenicall ...
PowerPoint-præsentation
PowerPoint-præsentation

... additional cases in Italy (2), the Netherlands (3) and Canada (4). The disorder is morphologically characterized by reduction of the birth weight to around 10 kg despite a gestation period of normal or slightly prolonged length, obvious shortening of the spine, long slender limbs, inferior brachygna ...
11.1 The Work of Gregor Mendel
11.1 The Work of Gregor Mendel

... A trait is a specific characteristic, such as (in peas) seed color or plant height. Mendel prevented self-pollination in the peas. He controlled fertilization so he could study how traits passed from one generation to the next. He created hybrids, which are crosses between true-breeding parents (the ...
myGridIntroduction - School of Computer Science
myGridIntroduction - School of Computer Science

... – Eliminated user bias and premature filtering of datasets and results leading to single sided, expert-driven hypotheses ...
Discriminate the Falsely Predicted Protein–Coding Genes in
Discriminate the Falsely Predicted Protein–Coding Genes in

... microbial genomes is far from thoroughly resolved, the annotation quality of microbial genomes has been questioned continuously [1, 2]. In most microbial genomes, it is found some annotated genes do not encode proteins actually, but rather open reading frames that occur by chance [2]. In recent work ...
Biol 1309 - Adaptations Adaptation – what does it mean?
Biol 1309 - Adaptations Adaptation – what does it mean?

... Promiscuous Proteins (enzymes) • Promiscuous enzymes usually do one job REALLY well and other jobs less well • There is a constraint to the evolution of new protein enzyme functions, thus an organism might benefit from a protein doing something new • The protein might carry out one process faster t ...
Study protocol to investigate the environmental and genetic
Study protocol to investigate the environmental and genetic

... 5. Marker selection FADS1, FADS2, FADS3 genes SNP selection in the FADS genes will be based on previous publications, previously reported association with LC-PUFA status or AD, and a minor allele frequency exceeding 10%. Since previous studies were performed in European rather than Asian populations ...
Searching for Novel Riboswitches in Newly Sequenced Genomes
Searching for Novel Riboswitches in Newly Sequenced Genomes

...  Classical Picture: DNA ...
Article Positive Selection Underlies Faster-Z
Article Positive Selection Underlies Faster-Z

... Faster-Z Evolution of Gene Expression We calculated the pairwise similarity in expression separately for each sex, using Spearman’s rho correlation coefficient () (Brawand et al. 2011; Meisel et al. 2012). We used pheasant as the reference point (i.e., comparing expression of each of the other five ...
Computational Biology
Computational Biology

... V21: Analysis of DNA methylation data Epigenetics refers to alternate phenotypic states that are not based on differences in genotype, and are potentially reversible, ...
Crossing Over and Gene Mapping
Crossing Over and Gene Mapping

... genes C and D, then we conclude that the distance between A and B on the chromosome must be twice the distance between C and D. Reasoning about Gene Mapping: Two Factor Cross In real life, we do not directly observe the crossovers occurring as they are depicted in Figs. 3-5. Instead we perform a tes ...
Use of mass spectrometry-derived data to annotate nucleotide and
Use of mass spectrometry-derived data to annotate nucleotide and

... Box 4.Analysis of proteins by mass spectrometry • Peptide sequencing by tandem mass spectrometry Proteins are generally digested by trypsin to generate peptides that are then sequenced by mass spectrometry.The method used to sequence peptides is termed tandem mass spectrometry, in which the peptide ...
Use it or lose it: molecular evolution of sensory
Use it or lose it: molecular evolution of sensory

... followed by a relaxation of selective pressure on the gene that leads to disruption in the coding sequence; in some cases, duplicated genes were redundant and thus quickly eliminated from the genome, but in other cases they acquired a function only to become obsolete at a later time in evolution. By ...
1. Sequence analysis
1. Sequence analysis

... Protein secondary structure. HELICALWHEEL is used to display the arrangement of residues in an -helical structure. Create the sequence "LRKQF KEMKK MMKQM TNMS" with the SeqLab editor and examine it with HELICALWHEEL. PEPTIDESORT. PEPTIDESORT examines an amino acid sequence for proteolytic cleavage ...
Importance of Genetic Studies in Consanguineous Populations for
Importance of Genetic Studies in Consanguineous Populations for

... There are over 7500 disorders with a known or suspected Mendelian basis, and 4473 have had their molecular basis determined (from Online Mendelian Inheritance in Man, OMIM; statistics true as of 23-06-15) (Clamp et al., 2007; Online Mendelian Inheritance in Man, 2013). However, over half of these ar ...
Genomics of Theileria parva
Genomics of Theileria parva

... • BLAST programs (there are several) compare a query sequence to all the sequences in a database in a pairwise manner. • Breaks: query and database sequences into fragments known as "words", and seeks matches between them. • Attempts to align query words of length "W" to words in the database such t ...
Accompanying Powerpoint Presentation
Accompanying Powerpoint Presentation

Linkage analysis
Linkage analysis

...  Replication in other case-control samples with AMD and estimated to be responsible for 43% of all the genetic contribution to the disease  CFH protein is found in retinal tissue, protecting against inflammation and the resulting accumulation of extracellular protein. The Tyr402His variant of the ...
Interference Do crossovers interefere with one another? Or, if a
Interference Do crossovers interefere with one another? Or, if a

... linkage is calculated (the so-called odds ratios) and then simply take the log10 of the number. The r value corresponding to the greatest Lod score, provides the best estimate of the r. The Lod scores can simply be added up across a number of pedigrees. If the Lod score exceeds 3 (this means probabi ...
DNA Methylation of Imprinted Loci on Autosomal Chromosomes and
DNA Methylation of Imprinted Loci on Autosomal Chromosomes and

... Genomic imprinting is an epigenetic phenomenon that results in the expression of either the maternally or paternally inherited allele of a subset of genes [1]. In humans, alterations of imprinting patterns gives rise to numerous diseases with well characterized growth phenotypes (Beckwith-Wiedemann ...
Take home quiz (due Monday April 4th)
Take home quiz (due Monday April 4th)

... Evolution Quiz Standard 1.2a and 1.2b In 2007 Sarah Tishkoff studied populations of humans from all over the world trying to determine if the ability to digest lactose was an adaptation that was selective in times of starvation. As environments changed and food was scarce, were humans with a mutatio ...
Aipotu Part III: Molecular Biology
Aipotu Part III: Molecular Biology

... this sequence and proceeds to the this sequence. right. therefore, a gene would look like this: 5’-TATAAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGGGGG-3’ ...
Ch 14 Lecture
Ch 14 Lecture

... D. Technology is providing new tools for genetic testing and counseling. 1. Many hospitals have genetic counselors that can provide information to prospective parents who are concerned about a family history of a specific disease. 2. Using Mendelian probability (Punnett Squares), one can determine ...
PDF - Journal of Cancer Metastasis and Treatment
PDF - Journal of Cancer Metastasis and Treatment

... relationships between AA, AG and GG polymorphisms of CTLA-4 gene and breast cancer-related factors. Results revealed that the frequency of AA and GG genotypes in breast cancer patients is higher than in controls, while AG genotype is more frequent in healthy controls. These results confirm the findi ...
Tutorial How to present scientific work
Tutorial How to present scientific work

... Variations between sequencing platforms And detection of splice variants ...
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RNA-Seq



RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.
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