Revealing the genetic roots of obesity and type 2 diabetes
... from. Inspection of these interactions learns that the predicted interactions between PPARG, TCF7L2 and HHEX are based on the fact that Gene Ontology (GO) terms are shared between these genes. As this program uses existing databases, and does not generate experimental evidence, results should be int ...
... from. Inspection of these interactions learns that the predicted interactions between PPARG, TCF7L2 and HHEX are based on the fact that Gene Ontology (GO) terms are shared between these genes. As this program uses existing databases, and does not generate experimental evidence, results should be int ...
Gene Section ACVR2A (activin A receptor, type IIA) in Oncology and Haematology
... Upon binding activin, ACVR2 associates with and phosphorylates ACVR1. ACVR1, in turn, phosphorylates Smad2 and/or Smad3. Phosphorylated Smad2 and Smad3 associate with Smad4, translocate to the nucleus, and regulate gene expression. There may be other non-Smad pathways in activin signal transduction. ...
... Upon binding activin, ACVR2 associates with and phosphorylates ACVR1. ACVR1, in turn, phosphorylates Smad2 and/or Smad3. Phosphorylated Smad2 and Smad3 associate with Smad4, translocate to the nucleus, and regulate gene expression. There may be other non-Smad pathways in activin signal transduction. ...
Gene Regulation and Biotechnology of Drought Tolerance in Rice
... With the rapid advancement in recombinant DNA technology, development of genetically modified plants for improving the value of crops by addition/deletion of selected gene(s) seems to a viable alternative or supplementary option of crop improvement compared to traditional or marker-assisted breeding ...
... With the rapid advancement in recombinant DNA technology, development of genetically modified plants for improving the value of crops by addition/deletion of selected gene(s) seems to a viable alternative or supplementary option of crop improvement compared to traditional or marker-assisted breeding ...
Extent of Gene Duplication in the Genomes of
... smaller than those of Rubin et al. (2000) because we conducted a very detailed database cleaning, and our criteria for homology are much more rigorous than theirs (they considered two proteins homologous if the BLASTP E value between them is #1026 but did not require any minimal length of the aligna ...
... smaller than those of Rubin et al. (2000) because we conducted a very detailed database cleaning, and our criteria for homology are much more rigorous than theirs (they considered two proteins homologous if the BLASTP E value between them is #1026 but did not require any minimal length of the aligna ...
Common polygenic variation contributes to risk of
... value, which could be much higher. Figure 3b shows seven examples from the range of consistent models, detailed in Supplementary Table 18. The simulated models consistent with our observed results all indicated a substantial number of common variants, whereas models that invoked only a few common va ...
... value, which could be much higher. Figure 3b shows seven examples from the range of consistent models, detailed in Supplementary Table 18. The simulated models consistent with our observed results all indicated a substantial number of common variants, whereas models that invoked only a few common va ...
Properties and sequence of the coenzyme B12
... The resulting recombinant plasmid designated pLM3 was used to transform E. coli K38/pGP1-2, which contains on the plasmid pGP1-2 bacteriophage T7 RNA polymerase under control of the VpL promoter and the temperature-sensitive cI857 V repressor. Expression of the genes was induced by a shift in temper ...
... The resulting recombinant plasmid designated pLM3 was used to transform E. coli K38/pGP1-2, which contains on the plasmid pGP1-2 bacteriophage T7 RNA polymerase under control of the VpL promoter and the temperature-sensitive cI857 V repressor. Expression of the genes was induced by a shift in temper ...
Divergent evolution of oxidosqualene cyclases in plants
... OSC genes (Inagaki et al., 2011). One of these (Os02g04710 ⁄ OsOSC2) encodes cycloartenol synthase (CS), while a further two (Os11g08569 ⁄ OsOSC7 and Os11g18194 ⁄ OsOSC8) have been shown to synthesize the triterpenes, parkeol and achilleol B, respectively, in Saccharomyces cerevisiae GIL77 (Ito et a ...
... OSC genes (Inagaki et al., 2011). One of these (Os02g04710 ⁄ OsOSC2) encodes cycloartenol synthase (CS), while a further two (Os11g08569 ⁄ OsOSC7 and Os11g18194 ⁄ OsOSC8) have been shown to synthesize the triterpenes, parkeol and achilleol B, respectively, in Saccharomyces cerevisiae GIL77 (Ito et a ...
CRACKING THE GENETIC CODE
... amino acids, beginning protein synthesis. The nascent protein chain is elongated by the subsequent binding of additional tRNAs and formation of a peptide bond between the incoming amino acid and the end of the growing chain. Although this general process was understood, the question remained: How do ...
... amino acids, beginning protein synthesis. The nascent protein chain is elongated by the subsequent binding of additional tRNAs and formation of a peptide bond between the incoming amino acid and the end of the growing chain. Although this general process was understood, the question remained: How do ...
Divergent evolution of oxidosqualene cyclases in plants
... OSC genes (Inagaki et al., 2011). One of these (Os02g04710 ⁄ OsOSC2) encodes cycloartenol synthase (CS), while a further two (Os11g08569 ⁄ OsOSC7 and Os11g18194 ⁄ OsOSC8) have been shown to synthesize the triterpenes, parkeol and achilleol B, respectively, in Saccharomyces cerevisiae GIL77 (Ito et a ...
... OSC genes (Inagaki et al., 2011). One of these (Os02g04710 ⁄ OsOSC2) encodes cycloartenol synthase (CS), while a further two (Os11g08569 ⁄ OsOSC7 and Os11g18194 ⁄ OsOSC8) have been shown to synthesize the triterpenes, parkeol and achilleol B, respectively, in Saccharomyces cerevisiae GIL77 (Ito et a ...
lecture05_11
... • When searching for a motif in a genome using PSSM or other methods – the motif is usually found all over the place ->The motif is considered real if found in the vicinity of a gene. • Checking experimentally for the binding sites of a specific TF (location analysis) – the sites that bind the motif ...
... • When searching for a motif in a genome using PSSM or other methods – the motif is usually found all over the place ->The motif is considered real if found in the vicinity of a gene. • Checking experimentally for the binding sites of a specific TF (location analysis) – the sites that bind the motif ...
and GvpD-mediated transcription regulation of the p
... pA promoters were constructed by recombinant PCR, fused to the bgaH reading frame and inserted into pWL102. The desired 4 nt mutations were introduced using pA-bgaH in pBSIISK+ as template. The oligonucleotides used for this procedure are summarized in Table 1. Two PCR reactions were performed to am ...
... pA promoters were constructed by recombinant PCR, fused to the bgaH reading frame and inserted into pWL102. The desired 4 nt mutations were introduced using pA-bgaH in pBSIISK+ as template. The oligonucleotides used for this procedure are summarized in Table 1. Two PCR reactions were performed to am ...
Chromosome Structure Variations
... Most cases of Down syndrome, trisomy-21, are spontaneous. They are caused by non-disjunction which gives an egg or sperm with two copies of chromosome 21. However, about 5% of Down’s cases are caused by a translocation between chromosome 21 and chromosome 14. These translocational Down’s cases are h ...
... Most cases of Down syndrome, trisomy-21, are spontaneous. They are caused by non-disjunction which gives an egg or sperm with two copies of chromosome 21. However, about 5% of Down’s cases are caused by a translocation between chromosome 21 and chromosome 14. These translocational Down’s cases are h ...
Translation - SBI4u Biology Resources
... can be seen as the decoding of instructions for making proteins, involving mRNA in transcription as well as tRNA. The genes in DNA encode protein molecules, which are the "workhorses" of the cell, carrying out all the functions necessary for life. For example, enzymes, including those that metaboliz ...
... can be seen as the decoding of instructions for making proteins, involving mRNA in transcription as well as tRNA. The genes in DNA encode protein molecules, which are the "workhorses" of the cell, carrying out all the functions necessary for life. For example, enzymes, including those that metaboliz ...
CHROMOSAL MUTATIONS SUBSTITUTION
... • Some mutations in the genome lead to the developme nt of diseases that may be inherited. Example: Cystic Fibrosis disease is an example of Negative (deleteriou s) Side Effect. Mutations in the Cystic Fibrosis Transmem brane Regulator (CFTR) gene that res~lt in Cystic Fibrosis can be passed on from ...
... • Some mutations in the genome lead to the developme nt of diseases that may be inherited. Example: Cystic Fibrosis disease is an example of Negative (deleteriou s) Side Effect. Mutations in the Cystic Fibrosis Transmem brane Regulator (CFTR) gene that res~lt in Cystic Fibrosis can be passed on from ...
De Novo Mutation Found in the Porphobilinogen Deaminase Gene
... the existence of the mutation. To investigate the impact of the mutation on the protein structure and to study further functional consequences, we decided to purify protein expressed from mutated gene in the procaryotic system. We isolated mRNA from the peripheral leukocytes, produced cDNA of PBGD b ...
... the existence of the mutation. To investigate the impact of the mutation on the protein structure and to study further functional consequences, we decided to purify protein expressed from mutated gene in the procaryotic system. We isolated mRNA from the peripheral leukocytes, produced cDNA of PBGD b ...
E1. Mexican hairless dogs are heterozygous for a dominant allele
... E7. The sandy variation may be due to a homozygous recessive allele at one of two different genes in these two varieties of sandy pigs. Let’s call them genes A and B. One variety of sandy pig could be aaBB and the other AAbb. The F1 generation in this cross would be heterozygotes for both genes and ...
... E7. The sandy variation may be due to a homozygous recessive allele at one of two different genes in these two varieties of sandy pigs. Let’s call them genes A and B. One variety of sandy pig could be aaBB and the other AAbb. The F1 generation in this cross would be heterozygotes for both genes and ...
Chapter 3 Clustering Microarray Data
... genes may be represented by a gene cluster and an associated subset of the samples which distinguishes the cluster. If the samples in the data set were taken over time, then gene clusters should be based on all the samples, but it may be more appropriate to use a clustering method that is designed f ...
... genes may be represented by a gene cluster and an associated subset of the samples which distinguishes the cluster. If the samples in the data set were taken over time, then gene clusters should be based on all the samples, but it may be more appropriate to use a clustering method that is designed f ...
Cloning and Expression of Endoglucanase genes from Trichoderma
... C loning and Sequence analysis of β-1, 6endoglucanase: U nder the experimental conditions described, a specific band of 1284bp was successfully amplified from T. harzianum chromosomal DNA, which was confirmed by sequencing. This band was subcloned into pTZ57R/T cloning vector, clones were confirmed ...
... C loning and Sequence analysis of β-1, 6endoglucanase: U nder the experimental conditions described, a specific band of 1284bp was successfully amplified from T. harzianum chromosomal DNA, which was confirmed by sequencing. This band was subcloned into pTZ57R/T cloning vector, clones were confirmed ...
Document
... E7. The sandy variation may be due to a homozygous recessive allele at one of two different genes in these two varieties of sandy pigs. Let’s call them genes A and B. One variety of sandy pig could be aaBB and the other AAbb. The F1 generation in this cross would be heterozygotes for both genes and ...
... E7. The sandy variation may be due to a homozygous recessive allele at one of two different genes in these two varieties of sandy pigs. Let’s call them genes A and B. One variety of sandy pig could be aaBB and the other AAbb. The F1 generation in this cross would be heterozygotes for both genes and ...
Mol Biol Evol-2015-Lipinska-15
... al.. Sexual Dimorphism and the Evolution of Sex-Biased Gene Expression in the Brown Alga Ectocarpus. Molecular Biology and Evolution, Oxford University Press (OUP), 2015, 32 (6), ...
... al.. Sexual Dimorphism and the Evolution of Sex-Biased Gene Expression in the Brown Alga Ectocarpus. Molecular Biology and Evolution, Oxford University Press (OUP), 2015, 32 (6), ...
The molecular biology of sex determination and sexual development
... Poultry rearing in the UK has developed into an intensive industry, integrating meat and egg production to generate around 800 million broilers, 30 million egg layers and 9000 million eggs every year (DEFRA, 2001; International Egg Commission, 2001). The USA figures are approximately 10 times higher ...
... Poultry rearing in the UK has developed into an intensive industry, integrating meat and egg production to generate around 800 million broilers, 30 million egg layers and 9000 million eggs every year (DEFRA, 2001; International Egg Commission, 2001). The USA figures are approximately 10 times higher ...
T. congolense - Centre for Genomic Research
... In four out of five systems tested gene expression was consistent with C57BL/6 mice having the most severe anaemia. The exception was enzymes for catabolism of erythrocyte proteins which were most upregulated in AJ mice. The overall expression patterns would also have predicted that AJ mice would ha ...
... In four out of five systems tested gene expression was consistent with C57BL/6 mice having the most severe anaemia. The exception was enzymes for catabolism of erythrocyte proteins which were most upregulated in AJ mice. The overall expression patterns would also have predicted that AJ mice would ha ...
RNA-Seq
RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.