USMLE Step 1 Web Prep — Transcription and RNA Processing: Part
... inside the nucleus to form the mature mRNA molecule A 7-methylguanosine cap is added to the 5' end while the RNA molecule is still being synthesized. The cap structure serves as a ribosome-binding site and also helps to protect the mRNA chain from degradation. A poly-A tail is attached to the 3' end ...
... inside the nucleus to form the mature mRNA molecule A 7-methylguanosine cap is added to the 5' end while the RNA molecule is still being synthesized. The cap structure serves as a ribosome-binding site and also helps to protect the mRNA chain from degradation. A poly-A tail is attached to the 3' end ...
Comparing Different Linear Expression Systems
... A promoter for any of these genes is put next to the gene for rtTA; transcription of the gene rtTA is activated. rtTA makes a tetracycline activator, but with a twist: it only works when doxycycline is present. Then, dox and the tet activator form a complex, and they bind to a special promoter, cons ...
... A promoter for any of these genes is put next to the gene for rtTA; transcription of the gene rtTA is activated. rtTA makes a tetracycline activator, but with a twist: it only works when doxycycline is present. Then, dox and the tet activator form a complex, and they bind to a special promoter, cons ...
2-Familial adenomatous polyposis coli
... surrounding this approach to therapy. In mammalian model species, such cells can be taken and used to regenerate differentiated tissue cells, such as in heart and brain. They have the ability to produce any cell in the body and proliferate rapidly in culture, and so could be used to refashion damage ...
... surrounding this approach to therapy. In mammalian model species, such cells can be taken and used to regenerate differentiated tissue cells, such as in heart and brain. They have the ability to produce any cell in the body and proliferate rapidly in culture, and so could be used to refashion damage ...
The making of the Fittest: Natural Selection and Adaptation
... provide a selective advantage in environments with large predatory fish, but are a liability in environments with dragonfly larvae. All the cells of the body contain the same genes, but only a subset of those genes is active in any particular cell type. For example, liver cells express a different s ...
... provide a selective advantage in environments with large predatory fish, but are a liability in environments with dragonfly larvae. All the cells of the body contain the same genes, but only a subset of those genes is active in any particular cell type. For example, liver cells express a different s ...
High-throughput Biology in the Postgenomic Era
... copy number changes are characteristic of many different cancers, these arrays were devised to rapidly assess which chromosomal segments are highly replicated or lost relative to background DNA (8). CGH arrays are often designed with cDNA probes periodically spaced across the genome while avoiding h ...
... copy number changes are characteristic of many different cancers, these arrays were devised to rapidly assess which chromosomal segments are highly replicated or lost relative to background DNA (8). CGH arrays are often designed with cDNA probes periodically spaced across the genome while avoiding h ...
Life or Cell Death: Deciphering c-Myc Regulated Gene Networks In
... – Quality control of microarray data – Several outliers but generally good quality data – Outliers increase variance – Remove for differential analysis – Outliers spread nicely amongst conditions – importance of randomisation! – Analysis of early time points – Direct c-Myc targets ...
... – Quality control of microarray data – Several outliers but generally good quality data – Outliers increase variance – Remove for differential analysis – Outliers spread nicely amongst conditions – importance of randomisation! – Analysis of early time points – Direct c-Myc targets ...
Comparative Genomics 2015 File
... The cladogram diagram below shows the relationship of selected animals based on their shared anatomical features. For example: out of seven key traits, all of these animals have a dorsal nerve cord, but only humans, monkeys and kangaroos have mammary glands. ...
... The cladogram diagram below shows the relationship of selected animals based on their shared anatomical features. For example: out of seven key traits, all of these animals have a dorsal nerve cord, but only humans, monkeys and kangaroos have mammary glands. ...
Inferring causal genomic alterations in breast cancer using gene
... include not only well-known oncogenes but also a number of novel cancer susceptibility genes validated via siRNA experiments Conclusion: the first effort to systematically identify and valid ate drivers for expression based CNV regions in breast cancer can be applied to many other large-scal ...
... include not only well-known oncogenes but also a number of novel cancer susceptibility genes validated via siRNA experiments Conclusion: the first effort to systematically identify and valid ate drivers for expression based CNV regions in breast cancer can be applied to many other large-scal ...
Postdoctoral position (2 years) in molecular cancer biology
... Background: A two years post-doctoral position is available at the GIGA-Research Institute of the University of Liege, Unit of Molecular Biology of Diseases (www.giga.ulg.ac.be). The candidate will conduct post-doctoral research in molecular cancer biology as part of an emerging team. By using a com ...
... Background: A two years post-doctoral position is available at the GIGA-Research Institute of the University of Liege, Unit of Molecular Biology of Diseases (www.giga.ulg.ac.be). The candidate will conduct post-doctoral research in molecular cancer biology as part of an emerging team. By using a com ...
oncogenes
... translocation of the immunoglobulin gene on chromosome 14 to the c-myc oncogene locus on chromosome 8 c-myc is under regulatory control of IgH resulting in overexpression of the oncogene ...
... translocation of the immunoglobulin gene on chromosome 14 to the c-myc oncogene locus on chromosome 8 c-myc is under regulatory control of IgH resulting in overexpression of the oncogene ...
Gene Expression Overview
... Beginners; and For every laboratory worker and everyone passion for learning ...
... Beginners; and For every laboratory worker and everyone passion for learning ...
E. coli
... 1. Prepare first-strand ss cDNA of two samples 2. PCR with α32P-dATP, 3 low-stringency cycles and 22-25 high-stringency cycles. 3. 5% non-denaturing PAGE and autoradiography 4. Clone the fragments different with the two samples. ...
... 1. Prepare first-strand ss cDNA of two samples 2. PCR with α32P-dATP, 3 low-stringency cycles and 22-25 high-stringency cycles. 3. 5% non-denaturing PAGE and autoradiography 4. Clone the fragments different with the two samples. ...
MATLAB script to run ISOpure-S1 % ISOpure
... % loglikelihood: log likelihood of the final model % S1model: a structure with the following important fields: % S1model.theta: an Nx(M+1) matrix, giving the fractional composition of each posttreatment profile. Each row represents a post-treatment sample that was part of the input. The first M colu ...
... % loglikelihood: log likelihood of the final model % S1model: a structure with the following important fields: % S1model.theta: an Nx(M+1) matrix, giving the fractional composition of each posttreatment profile. Each row represents a post-treatment sample that was part of the input. The first M colu ...
Objectives 2
... 1) List the types and principal functions of the nucleic acids. Deoxyribonucleic acid (DNA) is found in the cell nucleus and in the mitochondria and functions to store genetic information used for the synthesis of proteins and enzymes. Ribonucleic acid (RNA) is found in the nucleus, in the cytosol, ...
... 1) List the types and principal functions of the nucleic acids. Deoxyribonucleic acid (DNA) is found in the cell nucleus and in the mitochondria and functions to store genetic information used for the synthesis of proteins and enzymes. Ribonucleic acid (RNA) is found in the nucleus, in the cytosol, ...
DNA RNA Proteins
... Base substitution Also known as a point mutation Replacement of one nucleotide with another. Depending on how the base substitution is translated, it can result in no change in the protein (due to redundancy of genetic code), an insignficant change, or a change that significantly affects the ...
... Base substitution Also known as a point mutation Replacement of one nucleotide with another. Depending on how the base substitution is translated, it can result in no change in the protein (due to redundancy of genetic code), an insignficant change, or a change that significantly affects the ...
04/03
... Both enhancers and silencers affect transcription rate. Each has unique DNA sequence for the binding of regulatory proteins. Enhancer sequences contain multiple binding sites for trans-acting regulatory proteins. Enhancers could be located upstream from the promoter, downstream from the gene, or eve ...
... Both enhancers and silencers affect transcription rate. Each has unique DNA sequence for the binding of regulatory proteins. Enhancer sequences contain multiple binding sites for trans-acting regulatory proteins. Enhancers could be located upstream from the promoter, downstream from the gene, or eve ...
Supplementary Figure Legends
... 5’ and adds 325 bp to the 3’ UTR. We also annotated a canonical polyA-addition signal (AATAAA) at the extreme 3’ terminus of the last exon (not shown). ...
... 5’ and adds 325 bp to the 3’ UTR. We also annotated a canonical polyA-addition signal (AATAAA) at the extreme 3’ terminus of the last exon (not shown). ...
BSC 1010 Exam 3 Study Guide
... • What does it mean to ʻexpressʼ a gene? • Connection was made by linking a disease as result of a nonfunctioning enzyme to an altered gene • One Gene - One Protein Hypothesis: • The Central Dogma of Molecular Biology: Describes the flow in information from genes to protein ...
... • What does it mean to ʻexpressʼ a gene? • Connection was made by linking a disease as result of a nonfunctioning enzyme to an altered gene • One Gene - One Protein Hypothesis: • The Central Dogma of Molecular Biology: Describes the flow in information from genes to protein ...
Protein or gene
... progression of lymphomas and other tumors. Yu W, Inoue J, Imoto I, Matsuo Y, Karpas A, ...
... progression of lymphomas and other tumors. Yu W, Inoue J, Imoto I, Matsuo Y, Karpas A, ...
Genome Sequencing Using a Mapping Approach
... Shotgun Approach 1. The shotgun approach obtains a genomic sequence by breaking the genome into overlapping fragments for cloning and sequencing. 2. A computer is then used to assemble the genomic sequence. 3. Advances that have made this approach practical for large genomes include: a. Better compu ...
... Shotgun Approach 1. The shotgun approach obtains a genomic sequence by breaking the genome into overlapping fragments for cloning and sequencing. 2. A computer is then used to assemble the genomic sequence. 3. Advances that have made this approach practical for large genomes include: a. Better compu ...
Techniques
... _________________________ used for RNA and DNA separation ________________________ gel electrophoresis is used for protein separation ...
... _________________________ used for RNA and DNA separation ________________________ gel electrophoresis is used for protein separation ...
Joining the Dots: Network Analysis of Gene Perturbation Screens
... “What I cannot break, I do not understand.” ...
... “What I cannot break, I do not understand.” ...
DNA and Protein Synthesis
... adding a cap and tail consisting of extra nucleotides at the ends of the RNA transcript,-this protects RNA from cell enzymes removing introns (noncoding regions of the RNA), and RNA splicing, joining exons (the parts of the gene that are expressed) together to form messenger RNA (mRNA). ...
... adding a cap and tail consisting of extra nucleotides at the ends of the RNA transcript,-this protects RNA from cell enzymes removing introns (noncoding regions of the RNA), and RNA splicing, joining exons (the parts of the gene that are expressed) together to form messenger RNA (mRNA). ...
Study Guide
... 13. Fish are wild animals that we still harvest. Numerous fisheries have been seriously depleted and are in danger of collapse (tuna, swordfish and others) There used to be an abundance of Atlantic Cod off the coast of North America which has been drastically reduced (they have all been made into fi ...
... 13. Fish are wild animals that we still harvest. Numerous fisheries have been seriously depleted and are in danger of collapse (tuna, swordfish and others) There used to be an abundance of Atlantic Cod off the coast of North America which has been drastically reduced (they have all been made into fi ...
RNA-Seq
RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.