Formalin Penetration Rate
... i.e. to double the depth of penetration you must allow 4X the time and so doing halves the rate! Your 4mm tissue block would be fully penetrated in less than 1 hour (3.6 mm from each surface) What the issue really is, relates to the binding time necessary for formaldehyde. A 90% threshold binding, i ...
... i.e. to double the depth of penetration you must allow 4X the time and so doing halves the rate! Your 4mm tissue block would be fully penetrated in less than 1 hour (3.6 mm from each surface) What the issue really is, relates to the binding time necessary for formaldehyde. A 90% threshold binding, i ...
Question #1 - Jordan
... filament involves the hydrolysis of one ATP molecule. A myosin cross-bridge attaches to actin. ATP binding causes the dissociation of myosin from actin. In the absence of ATP (as occurs after death), myosin cannot dissociate from actin, and the muscles become stiff. This is known as rigor mortis. Th ...
... filament involves the hydrolysis of one ATP molecule. A myosin cross-bridge attaches to actin. ATP binding causes the dissociation of myosin from actin. In the absence of ATP (as occurs after death), myosin cannot dissociate from actin, and the muscles become stiff. This is known as rigor mortis. Th ...
Supplemental Data
... Gal80p-Gal80p interaction. Gal80p-Gal80p interactions were analyzed by complex formation with a Gal80p derivative with altered gel mobility. A fusion between Gal80p and the negatively charged activation domain of Herpes simplex VP16 (Gal80pVP16) migrates faster on native gels than Gal80p by itself. ...
... Gal80p-Gal80p interaction. Gal80p-Gal80p interactions were analyzed by complex formation with a Gal80p derivative with altered gel mobility. A fusion between Gal80p and the negatively charged activation domain of Herpes simplex VP16 (Gal80pVP16) migrates faster on native gels than Gal80p by itself. ...
Basic Enzyme Kinetics
... The reasons for this behavior is often related to the multimeric (multiple subunits) nature of regulated enzymes. Thus when a substrate binds to one of the subunits, a conformational change occurs which increases the binding affinity for the remaining vacant bindings on the other subunits. There are ...
... The reasons for this behavior is often related to the multimeric (multiple subunits) nature of regulated enzymes. Thus when a substrate binds to one of the subunits, a conformational change occurs which increases the binding affinity for the remaining vacant bindings on the other subunits. There are ...
... heteronuclear complexes of varying geometry. In addition, these complexes act as potential antimicrobial agents, catalysts2, anticancer agents, etc. The complexes have been found to show enhanced anticancer activity than that of ligands3. In the current study, we are reporting synthesis and characte ...
Abstract - WSU Horticulture
... mutants were drastically compromised in their affinity to calmodulin (CaM), but our unpublished data indicated that the synthetic-peptides corresponding to the mutated CaM-binding domains were able to interact with CaM. These results led us to hypothesize that there is an intramolecular interaction ...
... mutants were drastically compromised in their affinity to calmodulin (CaM), but our unpublished data indicated that the synthetic-peptides corresponding to the mutated CaM-binding domains were able to interact with CaM. These results led us to hypothesize that there is an intramolecular interaction ...
Rapid purification of heart muscle enzymes using dye affinity
... the ammonium sulphate fractionation. The affinity aqueous two-phase systems resulted in good recovery of both LDH and PK from the ammonium sulphate fraction, with purification factors that are consistent with conventional dye affinity column chromatography [3]. Previous workers have demonstrated dif ...
... the ammonium sulphate fractionation. The affinity aqueous two-phase systems resulted in good recovery of both LDH and PK from the ammonium sulphate fraction, with purification factors that are consistent with conventional dye affinity column chromatography [3]. Previous workers have demonstrated dif ...
NOTE Mixed-Ligand Complexes of Cu2+, Ni2+, Co2+, Zn2+ with 2,2
... of primary complex does not take place in the pH range 4-8. It can be considered that ABDA combines with species [M(Bipy)]2+ just as it does with [M(aq.)]2+ in simple system. As such the horizontal distance between the curve D and E can be used for the calculation of n MAL . The average number of AB ...
... of primary complex does not take place in the pH range 4-8. It can be considered that ABDA combines with species [M(Bipy)]2+ just as it does with [M(aq.)]2+ in simple system. As such the horizontal distance between the curve D and E can be used for the calculation of n MAL . The average number of AB ...
lecture07_13
... in one set but not in a random set - Use experimental information to rank the sequences according to their binding affinity and search for enriched motifs at the top of the list ...
... in one set but not in a random set - Use experimental information to rank the sequences according to their binding affinity and search for enriched motifs at the top of the list ...
Dephosphorylation of the Calcium Pump Coupled to Counterion
... Ca2+ release/H+ binding In the Ca2E2-P state, SERCA still has low-affinity Ca2+ binding site on the lumenal side. So, the calcium ions are deposited into the SR lumen with partial exchange of protons. As the Ca2+ dissociate, four side chain carboxyl groups that had previously been involved in bin ...
... Ca2+ release/H+ binding In the Ca2E2-P state, SERCA still has low-affinity Ca2+ binding site on the lumenal side. So, the calcium ions are deposited into the SR lumen with partial exchange of protons. As the Ca2+ dissociate, four side chain carboxyl groups that had previously been involved in bin ...
lecture 8
... GroEL/GroES system may bind 10% of all bacterial cytosolic proteins but recent study shows only a portion of those are completely chaperonin-dependent Belongs to so-called Group I chaperonins which includes evolutionarily-related bacterial GroEL, mitochondrial Hsp60, and chloroplast Rubisco subu ...
... GroEL/GroES system may bind 10% of all bacterial cytosolic proteins but recent study shows only a portion of those are completely chaperonin-dependent Belongs to so-called Group I chaperonins which includes evolutionarily-related bacterial GroEL, mitochondrial Hsp60, and chloroplast Rubisco subu ...
Methods for identifying microRNA binding motifs
... identification of recognition sequence, readout by short-read sequencing • Amenable to large-scale analysis but unable to differentiate between direct and indirect miRNA-target interactions ...
... identification of recognition sequence, readout by short-read sequencing • Amenable to large-scale analysis but unable to differentiate between direct and indirect miRNA-target interactions ...
Computer Aided Molecular Design
... Different from Pharmacy: distribution of pharmaceuticals, drug delivery systems ...
... Different from Pharmacy: distribution of pharmaceuticals, drug delivery systems ...
Substrate targeting mechanisms
... 1) Catalytic switching mechanisms -the ability to switch on and off in response to specific upstream signals 2) Substrate targeting mechanisms - The ability to select a restricted set of substrates in the cell any one time -15,000+ proteins in the genome - each with many phosphorylatable sites (ser/ ...
... 1) Catalytic switching mechanisms -the ability to switch on and off in response to specific upstream signals 2) Substrate targeting mechanisms - The ability to select a restricted set of substrates in the cell any one time -15,000+ proteins in the genome - each with many phosphorylatable sites (ser/ ...
Entering and leaving group effects in Oh ligand substitutions
... The NH2- ligand stabilizes a lower (5-coordinate) transition state, because it is both a good sigma donor and a good π-donor. this accelerates rate-determining step: loss of Cl– ion Reaction studies of 16O/18O isotope effects indicate that OH– in the product complex comes from bulk H2O, not from OH– ...
... The NH2- ligand stabilizes a lower (5-coordinate) transition state, because it is both a good sigma donor and a good π-donor. this accelerates rate-determining step: loss of Cl– ion Reaction studies of 16O/18O isotope effects indicate that OH– in the product complex comes from bulk H2O, not from OH– ...
Our work was originally motivated my collaboration with Drs
... Our work was originally motivated my collaboration with Drs Pasqualini & Arap who have been studying molecular targets that may be used to direct therapies to specific tissues. The long term goal of this kind of work is that if drugs can be targeted to specific tissues in the body, then dosage can b ...
... Our work was originally motivated my collaboration with Drs Pasqualini & Arap who have been studying molecular targets that may be used to direct therapies to specific tissues. The long term goal of this kind of work is that if drugs can be targeted to specific tissues in the body, then dosage can b ...
Antigenic structure of foot-and-mouth disease virus
... The icosahedral symmetry of F M D virions imposes restrictions on the bivalent binding of antibodies. It is interesting t o examine some possibilities for intra-virion binding of antibodies. The relationship of the pentameric subunits is illustrated in Fig. 1 and the viral proteins V P 1 , 2 and 3 h ...
... The icosahedral symmetry of F M D virions imposes restrictions on the bivalent binding of antibodies. It is interesting t o examine some possibilities for intra-virion binding of antibodies. The relationship of the pentameric subunits is illustrated in Fig. 1 and the viral proteins V P 1 , 2 and 3 h ...
Lecture 6 - TCD Chemistry
... Three sets of interaction based on symmetry of ligand AO’s – Generally applicable to σ bonding TM ligands ...
... Three sets of interaction based on symmetry of ligand AO’s – Generally applicable to σ bonding TM ligands ...
Slide 1
... 6.10 Modelling studies on ATP binding Cyclic AMP dependant protein kinase + Mg + ATP + Inhibitor (bound at substrate site) Crystallise Crystals X-Ray Crystallography Structure of protein / inhibitor / ATP complex Molecular modelling Identify active site and binding interactions for ATP ...
... 6.10 Modelling studies on ATP binding Cyclic AMP dependant protein kinase + Mg + ATP + Inhibitor (bound at substrate site) Crystallise Crystals X-Ray Crystallography Structure of protein / inhibitor / ATP complex Molecular modelling Identify active site and binding interactions for ATP ...
Genome evolution: a sequence
... In small genomes, most sites are in promoters, and there is a bias toward nucleosome free region near the TSS In larger genomes (fly) we observe CRM (cis-regulatory-modules) which are frequently away from the TSS. These represent enhancers. A single binding site, without the context of other co-site ...
... In small genomes, most sites are in promoters, and there is a bias toward nucleosome free region near the TSS In larger genomes (fly) we observe CRM (cis-regulatory-modules) which are frequently away from the TSS. These represent enhancers. A single binding site, without the context of other co-site ...
Functional Assay for Botulinum Neurotoxin Type A
... Figure 2: Capture Methods. Schematics showing the use of SV2c in the binding and detection of Botulinum ...
... Figure 2: Capture Methods. Schematics showing the use of SV2c in the binding and detection of Botulinum ...
Crystal structure and spectroscopic properties of [Zn(2-qmpe)Cl ] containing diethyl (quinolin-2-ylmethyl)phosphonate ligand (2-qmpe)
... acid and esters, can exhibit antibiotic, antibacterial, and antiviral and cytostatic activity [27–32]. However, some of their palladium and platinum halide complexes have been found to be cytostatic to various animal and human tumor cells [18, 24–35]. Similar behaviour may be expected in Zn(II) comp ...
... acid and esters, can exhibit antibiotic, antibacterial, and antiviral and cytostatic activity [27–32]. However, some of their palladium and platinum halide complexes have been found to be cytostatic to various animal and human tumor cells [18, 24–35]. Similar behaviour may be expected in Zn(II) comp ...
1 Introduction - diss.fu
... structure in the complex with ribosome only in respect to IF1 and a domain of IF3 (Carter et al., 2001; Pioletti et al., 2001). Probably the first initiation factor, which binds to the ribosome, is IF3. It has two identified functions. (1) It acts as an anti-association factor preventing formation o ...
... structure in the complex with ribosome only in respect to IF1 and a domain of IF3 (Carter et al., 2001; Pioletti et al., 2001). Probably the first initiation factor, which binds to the ribosome, is IF3. It has two identified functions. (1) It acts as an anti-association factor preventing formation o ...
A Statistical Method for Finding Transcriptional Factor Binding Sites
... Statistical significance of a motif (s) is based on the frequencies of shorter (more frequent) oligonucleotides ...
... Statistical significance of a motif (s) is based on the frequencies of shorter (more frequent) oligonucleotides ...
Cooperative binding
Molecular binding is an interaction between molecules that results in a stable physical association between those molecules. Cooperative binding occurs in binding systems that are constituted by more than one type (species) of molecule (say molecules A and B) and in which one of the partners is not mono-valent; i.e., it binds more than one molecule of the other molecular species. For example, one molecule of type A can bind 6 molecules of type B (in such cases, B is usually referred to as the ""ligand""). Binding in this type of system can be considered ""cooperative"" if the binding of B to one site on A is affected by the binding of B to other site(s) on A. In other words, the binding of B molecules to the different sites on A do not constitute mutually independent events. This can be due, for instance, to an affinity for the ligand that depends on the amount of ligand bound. Cooperativity can be positive or negative. Cooperative binding is observed in many biopolymers, including proteins and nucleic acids. Cooperative binding has been shown to be the mechanism underlying a large range of biochemical and physiological processes.