11th ROC: Diazoaminobenzene
... aniline (Mathews and De Costa 1999). The proposed metabolic pathway for DAAB is reductive cleavage by liver enzymes or by bacteria in the digestive tract to form benzene, aniline, and nitrogen. Benzene and aniline then are metabolized by cytochrome P-450 and conjugating enzymes. Electron spin resona ...
... aniline (Mathews and De Costa 1999). The proposed metabolic pathway for DAAB is reductive cleavage by liver enzymes or by bacteria in the digestive tract to form benzene, aniline, and nitrogen. Benzene and aniline then are metabolized by cytochrome P-450 and conjugating enzymes. Electron spin resona ...
The Family of Berberine Bridge Enzyme-like
... I heartily want to thank everyone who helped me in this project during the last four years. I want to express gratitude to my supervisor Prof. Peter Macheroux for giving me the opportunity to catch a tiny glimpse of the fascinating world of flavoproteins. I would like to thank him for his constant s ...
... I heartily want to thank everyone who helped me in this project during the last four years. I want to express gratitude to my supervisor Prof. Peter Macheroux for giving me the opportunity to catch a tiny glimpse of the fascinating world of flavoproteins. I would like to thank him for his constant s ...
Reactions of glycolysis and gluconeogenesis
... All four PK isozymes are homotetramers of 60 kDa subunits, and except for the M1 isozyme, require allosteric activation by fructose-1,6-bisphosphate for full activity. ...
... All four PK isozymes are homotetramers of 60 kDa subunits, and except for the M1 isozyme, require allosteric activation by fructose-1,6-bisphosphate for full activity. ...
Enzyme Mechanisms
... These are diffusion-controlled reactions. Ultra-high turnover rates: kcat ~ 109 s-1. We can describe kcat / Km as catalytic efficiency of an enzyme. A diffusioncontrolled reaction will have a catalytic efficiency on the order of 108 M-1s-1. 11/5/2009 Biochem: Enzymes IV ...
... These are diffusion-controlled reactions. Ultra-high turnover rates: kcat ~ 109 s-1. We can describe kcat / Km as catalytic efficiency of an enzyme. A diffusioncontrolled reaction will have a catalytic efficiency on the order of 108 M-1s-1. 11/5/2009 Biochem: Enzymes IV ...
Chemistry of Carbohydrates
... 2-Disaccharides... They are products of chemical reaction between two monosaccharides with loss of a molecule of water (can be hydrolyzed), the linkage between two monosaccharides known as glycosidic link. Examples of disaccharides are maltose, lactose & sucrose. If the glycosidic link between alde ...
... 2-Disaccharides... They are products of chemical reaction between two monosaccharides with loss of a molecule of water (can be hydrolyzed), the linkage between two monosaccharides known as glycosidic link. Examples of disaccharides are maltose, lactose & sucrose. If the glycosidic link between alde ...
Metabolism
... some glucose. • In the small intestine, pancreatic amylase hydrolyzes dextrins to maltose and glucose. • The disaccharides maltose, lactose, and sucrose are hydrolyzed to monosaccharides. • The monosaccharides enter the bloodstream for transport to the cells. Metabolism | # 2 ...
... some glucose. • In the small intestine, pancreatic amylase hydrolyzes dextrins to maltose and glucose. • The disaccharides maltose, lactose, and sucrose are hydrolyzed to monosaccharides. • The monosaccharides enter the bloodstream for transport to the cells. Metabolism | # 2 ...
Dominance and Its Evolution
... Explanations of dominance and its evolution Basic scenarios for the evolution of dominance Almost all population-genetic explanations for the evolution of dominance are based on scenarios involving selection. Distilled down to its simplest form, the common idea in these models is the notion that giv ...
... Explanations of dominance and its evolution Basic scenarios for the evolution of dominance Almost all population-genetic explanations for the evolution of dominance are based on scenarios involving selection. Distilled down to its simplest form, the common idea in these models is the notion that giv ...
Asthma Metabolomics: The Missing Step for Translating Bench Work
... clinical, environmental, genetic, and genomic data in conjunction with descriptive metabolic profiles obtained by MS, it is possible to describe patterns of changes and biomarkers that discriminate between states of asthma severity and asthmatic cases and controls [19,20]. Investigators have already ...
... clinical, environmental, genetic, and genomic data in conjunction with descriptive metabolic profiles obtained by MS, it is possible to describe patterns of changes and biomarkers that discriminate between states of asthma severity and asthmatic cases and controls [19,20]. Investigators have already ...
Glutathione Breakthrough: Advancement in
... Glutathione (GSH) is one of the most critical molecules of the entire body. GSH plays an important role in antioxidant defense, nutrient metabolism and is key in a vast number of cellular processes including gene expression, DNA and protein synthesis, cell proliferation and apoptosis, signal transdu ...
... Glutathione (GSH) is one of the most critical molecules of the entire body. GSH plays an important role in antioxidant defense, nutrient metabolism and is key in a vast number of cellular processes including gene expression, DNA and protein synthesis, cell proliferation and apoptosis, signal transdu ...
Hepatic Steatosis - Arteriosclerosis, Thrombosis, and Vascular Biology
... show liver steatosis.14,15 Conversely, mice lacking HSL have low plasma FFA levels and low hepatic TG content.16 Finally, muscle-specific modulation of lipoprotein lipase may result in altered distribution of tissue TGs. In mice with musclespecific LPL overexpression, muscle TG content is increased, ...
... show liver steatosis.14,15 Conversely, mice lacking HSL have low plasma FFA levels and low hepatic TG content.16 Finally, muscle-specific modulation of lipoprotein lipase may result in altered distribution of tissue TGs. In mice with musclespecific LPL overexpression, muscle TG content is increased, ...
Biochemistry 304 2014 Student Edition Glycolysis Lectures
... Pyruvate kinase controls the outflow from the glycolysis pathway. It is the third irreversible step. This final step yields ATP and pyruvate. Several mammalian isozymes of tetramer enzyme: L-form predominates in liver M-form predominates in muscle and brain ...
... Pyruvate kinase controls the outflow from the glycolysis pathway. It is the third irreversible step. This final step yields ATP and pyruvate. Several mammalian isozymes of tetramer enzyme: L-form predominates in liver M-form predominates in muscle and brain ...
6 Medium-Chain Acyl-Coenzyme A Dehydrogenase Deficiency
... The latter two tests can be used for prenatal diagnosis. Based on newborn screening results, approximately 50% of individuals are homozygous for the common mutation p.Lys304Glu, and approximately 40% are heterozygous for p.Lys304Glu and one of more than 90 rarer alleles. ...
... The latter two tests can be used for prenatal diagnosis. Based on newborn screening results, approximately 50% of individuals are homozygous for the common mutation p.Lys304Glu, and approximately 40% are heterozygous for p.Lys304Glu and one of more than 90 rarer alleles. ...
Lipase Specificity and Selectivity
... groups. A kinetic investigation of the reaction showed the product distribution with time when using HEA or HEMA with ε‐caprolactone or ω‐pentadecalactone. The high selectivity of CALB towards lactones over (meth)acrylate esters such as ethylene glycol di(meth)acrylate ...
... groups. A kinetic investigation of the reaction showed the product distribution with time when using HEA or HEMA with ε‐caprolactone or ω‐pentadecalactone. The high selectivity of CALB towards lactones over (meth)acrylate esters such as ethylene glycol di(meth)acrylate ...
A systems biology approach sheds new light on the regulation of
... In my years in Birmingham I had the pleasure to meet many friends and co-workers. I would like to thank them for being there to support me and I am sorry if I cannot mention all of them in this page. However, they will always be in my thoughts. The most special thanks are for my supervisor, Dr Franc ...
... In my years in Birmingham I had the pleasure to meet many friends and co-workers. I would like to thank them for being there to support me and I am sorry if I cannot mention all of them in this page. However, they will always be in my thoughts. The most special thanks are for my supervisor, Dr Franc ...
Mass Spectrometry in the Clinical Laboratory
... produce an abnormal protein with reduced or absent catalytic activity. If the defective enzyme plays a major role in the primary catabolic pathways of a substrate, then a significant reduction in that substrate’s metabolic rate will occur. Results of decreased enzymatic activity may produce substrat ...
... produce an abnormal protein with reduced or absent catalytic activity. If the defective enzyme plays a major role in the primary catabolic pathways of a substrate, then a significant reduction in that substrate’s metabolic rate will occur. Results of decreased enzymatic activity may produce substrat ...
Metabolism of Plasma Membrane Lipids in
... annually. The Corynebacterineae also includes non-pathogenic species such as Mycobacterium smegmatis, a saprophytic species, and Corynebacterium glutamicum, an industrial workhorse for the production of amino acids and other useful compounds. These relatively fast-growing species serve as useful mod ...
... annually. The Corynebacterineae also includes non-pathogenic species such as Mycobacterium smegmatis, a saprophytic species, and Corynebacterium glutamicum, an industrial workhorse for the production of amino acids and other useful compounds. These relatively fast-growing species serve as useful mod ...
Facultative Anaerobiosis in the Invertebrates: Pathways and Control
... substrates completely to CO2 and H2O; end-products of fermentation invariably accumulate, indicating either the complete absence or the presence of only a limited terminal respiratory pathway. The intestinal nematode, Ascaris lumbricoides, has served as a model system for the elucidation of an anaer ...
... substrates completely to CO2 and H2O; end-products of fermentation invariably accumulate, indicating either the complete absence or the presence of only a limited terminal respiratory pathway. The intestinal nematode, Ascaris lumbricoides, has served as a model system for the elucidation of an anaer ...
Results Introduction Method Conclusions Acyl migration
... formed via acyl migration can be separated. Furthermore the disappearance by hydrolysis can be differentiated from acyl migration. ...
... formed via acyl migration can be separated. Furthermore the disappearance by hydrolysis can be differentiated from acyl migration. ...
Slide
... A 41-year-old woman is evaluated during a follow-up visit for high blood pressure. On physical examination, blood pressure is 162/100 mm Hg, which is similar to the values measured at her initial visit. Other vital signs are normal. BMI is 21. Laboratory studies are normal. Which of the following i ...
... A 41-year-old woman is evaluated during a follow-up visit for high blood pressure. On physical examination, blood pressure is 162/100 mm Hg, which is similar to the values measured at her initial visit. Other vital signs are normal. BMI is 21. Laboratory studies are normal. Which of the following i ...
21. glycolysis
... details of these reactions and those of the enzymes, which catalyze them, are given below : Step 1 : Phosphorylation of Glucose In the first step, glucose is activated (or primed) for subsequent reactions by its phosphorylation at C6 to yield The terms used for glyceric acid, glucose 6-phosphate, us ...
... details of these reactions and those of the enzymes, which catalyze them, are given below : Step 1 : Phosphorylation of Glucose In the first step, glucose is activated (or primed) for subsequent reactions by its phosphorylation at C6 to yield The terms used for glyceric acid, glucose 6-phosphate, us ...
Metabolic network modelling
Metabolic network reconstruction and simulation allows for an in-depth insight into the molecular mechanisms of a particular organism. In particular, these models correlate the genome with molecular physiology. A reconstruction breaks down metabolic pathways (such as glycolysis and the Citric acid cycle) into their respective reactions and enzymes, and analyzes them within the perspective of the entire network. In simplified terms, a reconstruction collects all of the relevant metabolic information of an organism and compiles it in a mathematical model. Validation and analysis of reconstructions can allow identification of key features of metabolism such as growth yield, resource distribution, network robustness, and gene essentiality. This knowledge can then be applied to create novel biotechnology.In general, the process to build a reconstruction is as follows: Draft a reconstruction Refine the model Convert model into a mathematical/computational representation Evaluate and debug model through experimentation↑