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Three cheers for the goblet cell: maintaining homeostasis in
Three cheers for the goblet cell: maintaining homeostasis in

... highlighting differences in tissue-specific regulation of goblet cell differentiation. Loss of Klf5 in the embryonic intestine revealed severely disrupted crypt-villus architecture and lack of differentiated cell types, including goblet cells [74]. However, conditional loss of Klf4 at 2 weeks of age ...
ABSTRACT - Johns Hopkins University
ABSTRACT - Johns Hopkins University

... divided into >3 year disease free survival (DFS) and < 3 year DFS groups for analysis. The 3 year cut off was chosen because patients surviving disease free after 3 years are likely to remain disease free. These experiments identified several protein bands that patients in the > 3 year DFS group dev ...
Anatomy and Physiology I-BIO205 - Portal
Anatomy and Physiology I-BIO205 - Portal

... 13. describe the structure and function of the skin including accessory organs of the skin. 14. name the layers of the epidermis and describe the characteristics of each. 15. list and describe the level of burns based on severity from least to most and define the “Rule of Nines”. 16. describe the m ...
HIV-specific antibody- dependent cellular cytotoxicity
HIV-specific antibody- dependent cellular cytotoxicity

... an important bridge between the innate and acquired immune systems, wherein antibodies binding to antigen presented on infected cells and recruit natural killer (NK) cells to kill the virus-infected target. The Thai RV 144 Phase III vaccine trial conducted from 2003 to 2009, using 16,000 volunteers, ...
Identification of Immunogenic Human Melanoma Antigens in a
Identification of Immunogenic Human Melanoma Antigens in a

... may increase resistance to melanoma. The most convincing evidence that this concept is correct is that immunization to melanoma vaccines can prevent this cancer in syngeneic mice (2—4).The protection conferred is specific (2), i.e., mice immu nized with control vaccine are not protected against me ...
Document
Document

... into a number of smaller sinuses • It meanders through these sinuses and exits the node at the hilus via efferent vessels • Because there are fewer efferent vessels, lymph stagnates somewhat in the node • This allows lymphocytes and macrophages time to carry out their protective functions Copyright ...
Licentiate-thesis from the Department of Immunology, Wenner-Gren Institute, Stockholm University, Sweden
Licentiate-thesis from the Department of Immunology, Wenner-Gren Institute, Stockholm University, Sweden

... and physiological barriers, like the skin and temperature, as well as innate immune cells1. These cells respond rapidly by phagocytosing pathogens and by secreting substances like chemokines and cytokines which attract and activate other cells. The adaptive immune system is slower, reacts to specifi ...
ADC Immunogenicity Testing Strategy
ADC Immunogenicity Testing Strategy

... compounds, and antibody-drug conjugates, is understanding how the human immune system will respond. As we advance more of these novel therapeutics we will have the opportunity, through careful characterization of antibodies that are generated, to better understand how to produce less immunogenic com ...
Neutropenia and myeloma
Neutropenia and myeloma

... of the first signs of infection. Therefore it’s a good idea to get a thermometer so that you can take your temperature if you suspect that you have an infection (your clinic should provide you with one of these). Future directions Treatment of the underlying myeloma, taking preventative measures and ...
Doctoral thesis from the Department of Immunology, the Wenner-Gren Institute,... University, Stockholm, Sweden
Doctoral thesis from the Department of Immunology, the Wenner-Gren Institute,... University, Stockholm, Sweden

... In most infected individuals, who do not develop active TB, a delicate balance is established between the host immune response and the M. tuberculosis virulence, which is termed ‘granuloma formation’ (14, 15). The structure of granulomas is a cluster of M. tuberculosis living inside macrophages surr ...
Optimizing Intracellular Flow Cytometry
Optimizing Intracellular Flow Cytometry

... epithelial cells and resident macrophages, especially near the interface with the external environment • Critical to the development and functioning of both the innate and adaptive immune responses • Promote cellular differentiation and proliferation – Example: IL-2 involved in T cell activation and ...
Wiskott-Aldrich syndrome protein–mediated actin dynamics control
Wiskott-Aldrich syndrome protein–mediated actin dynamics control

... ne = not evaluable. MxA is expressed as the percentage of reactivity of the spleen area. pDCs are expressed as BDCA-2+ cells/mm2spleen area. Absolute pDC count and MxA expression were quantified on spleen sections of WAS patients. The MxA score was evaluated by counting the percentage of MxA+ cells ...
and Progression of Acute Allograft Rejection Cardiac Grafts
and Progression of Acute Allograft Rejection Cardiac Grafts

... promote acute allograft rejection remain largely unknown. To better understand temporal aspects of early inflammatory events in vascularized organ grafts, we tested the intragraft expression of four different chemokines in heterotopically transplanted A/J (H-2a) and syngeneic heart grafts in C57BL/6 ...
Chapter Twenty Four
Chapter Twenty Four

... • The white pulp is associated with the arterial supply and consists of T- and B-lymphocytes and macrophages. • In the center of each cluster is a central artery. • The red pulp is associated with the venous supply. • Red pulp consists of splenic cords and splenic sinusoids that contain erythrocytes ...
Regulatory T cells - TARA
Regulatory T cells - TARA

... shown to be mediated either through secretion of immunosuppressive cytokines or by cell-tocell contact (FIG. 3). Many studies have demonstrated that suppression mediated by Tr1 or Th3 cells can be reversed using anti-IL-10 or anti-TGF- antibodies. IL-10 inhibits TNF- and IL-12 production, whereas ...
Wellness Foods Based on the Health Benefits of Fruit: Gold Kiwifruit
Wellness Foods Based on the Health Benefits of Fruit: Gold Kiwifruit

... cell-based assays and animal models and validating health benefits in human trials. Using ZESPRI® GOLD Kiwifruit as an example, extracts stimulated human innate and adaptive immunity in vitro and a puree, but not a juice, enhanced a weak gut-associated immune response in mice. To validate an associa ...
Human Pancreatic Cancer Cells Induce a MyD88
Human Pancreatic Cancer Cells Induce a MyD88

... circulating effector memory Th17:Th1 ratios compared with healthy controls. The TAS response also directly suppressed CD8þ T-cell–mediated cytotoxicity. Overall, our results demonstrate how TAS contributes to the production of an immunosuppressive tumor microenvironment in pancreatic cancer. ...
papillomaviruses and cancer: from basic studies to
papillomaviruses and cancer: from basic studies to

... progression of HPV-linked tumours (FIG. 4). In addition, two other modes of control exist in proliferating cells that are infected by HPV to protect them against malignant transformation: one involves inhibition of viral oncoprotein function, the other involves transcriptional control (FIG. 4). The ...
Immunomodulatory Effects of Human Immunodeficiency
Immunomodulatory Effects of Human Immunodeficiency

... infection as the virus has adapted myriad ways to remain persistent in the host where it causes severe damage to the immune system. Both humoral and cellular immune responses are mounted against HIV-1 during the initial phase of infection but fail to control viral replication as these responses are ...
Characterization of CD56 CD16 natural killer (NK) cells: A highly
Characterization of CD56 CD16 natural killer (NK) cells: A highly

... into two different groups: (i) killer immunoglobulin-like receptors (KIRs); (ii) LIR1兾ILT2 and NKG2A兾CD94, which belong to the family of the C-type lectin proteins. Diminution or absence of expression of HLA-I molecules on the cell surface because of viral infection or tumor transformation results i ...
Kidney-in-Pregnancy
Kidney-in-Pregnancy

... Afferent Local PG's TGF ...
2015 Annual Report
2015 Annual Report

... incredibly talented schoolchildren from around the world ...
Expression of CD1d in human scalp skin and hair follicles: hair cycle
Expression of CD1d in human scalp skin and hair follicles: hair cycle

... Conclusions: These results suggest that CD1d plays a role in human scalp skin immunology and protection against lipid antigen rich infectious microbes. They also raise the question of whether keratinocytes of the immunoprivileged anagen hair follicle can present lipid antigens to natural killer T ce ...
Mammalogy Lecture 5 -- Disparity in Diversity between Marsupials
Mammalogy Lecture 5 -- Disparity in Diversity between Marsupials

... So today, we’ll discuss two hypotheses regarding the primary reason for this, and both have to do with reproduction. A. Trophoblast Hypothesis - The principle difference is the trophoblast, part of the embryonic contribution to the placenta that is well-developed in eutherians, but not metatherians. ...
INTRODUCTION CHAPTER 1
INTRODUCTION CHAPTER 1

... affects the immune system depleting it of crucial T helper cells (CD4+ lymphocytes) needed by both the humoral and cellular arms of the immune system, thereby rendering the individual immunocompromised. This depletion leads to an AIDS state characterised by wasting, morbidity, a host of opportunisti ...
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Immunomics

Immunomics is the study of immune system regulation and response to pathogens using genome-wide approaches. With the rise of genomic and proteomic technologies, scientists have been able to visualize biological networks and infer interrelationships between genes and/or proteins; recently, these technologies have been used to help better understand how the immune system functions and how it is regulated. Two thirds of the genome is active in one or more immune cell types and less than 1% of genes are uniquely expressed in a given type of cell. Therefore, it is critical that the expression patterns of these immune cell types be deciphered in the context of a network, and not as an individual, so that their roles be correctly characterized and related to one another. Defects of the immune system such as autoimmune diseases, immunodeficiency, and malignancies can benefit from genomic insights on pathological processes. For example, analyzing the systematic variation of gene expression can relate these patterns with specific diseases and gene networks important for immune functions.Traditionally, scientists studying the immune system have had to search for antigens on an individual basis and identify the protein sequence of these antigens (“epitopes”) that would stimulate an immune response. This procedure required that antigens be isolated from whole cells, digested into smaller fragments, and tested against T- and B-cells to observe T- and B- cell responses. These classical approaches could only visualize this system as a static condition and required a large amount of time and labor.Immunomics has made this approach easier by its ability to look at the immune system as a whole and characterize it as a dynamic model. It has revealed that some of the immune system’s most distinguishing features are the continuous motility, turnover, and plasticity of its constituent cells. In addition, current genomic technologies, like microarrays, can capture immune system gene expression over time and can trace interactions of microorganisms with cells of the innate immune system. New, proteomic approaches, including T-cell and B-cells-epitope mapping, can also accelerate the pace at which scientists discover antibody-antigen relationships.
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