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Cytoskeleton Handout
Cytoskeleton Handout

... Actin participates in force generation (eg., cell motility) via interactions with myosin. Myosin is an actin-activated ATPase that converts chemical energy (i.e., ATP) into mechanical energy by moving along microfilaments (or by moving the actin filaments). Cellular motility is a dynamic process inv ...
Introduction to bioinformatics
Introduction to bioinformatics

... http://www.biochem.ucl.ac.uk/~robert/bioinf/lecture1/index.html http://www.biochem.ucl.ac.uk/~robert/bioinf/lecture2/index.html ...
ready for
ready for

... two β strands. It is a two-step folding process in which first the intervening chain folds against one β strand, and then the second β strand folds down next to the first. • A characteristic which enables the shown pathway to prejudice handedness is the condition that the ends of the folding section ...
Structure of a Generalized Cell
Structure of a Generalized Cell

... • The cell is the basic structural and functional unit of life. – The cells structural anatomy will determine its overall function. • All cells contains organelles which are the equivalent to organs of an organism. – They provide a specific function within the cell. • produces different types of pro ...
stemFit Ingredients the Perfect Super Foods. and the links below are
stemFit Ingredients the Perfect Super Foods. and the links below are

... and the links below are great introductions to the science 1. Fertilized Egg Extract Young Tissue Extract™ (YTE) is an extract from incubated fertilized eggs, and contains 82-83% growth factor signaling proteins not found or available from any other food sources. The production process is critical t ...
Structure of a Generalized Cell
Structure of a Generalized Cell

... • The cell is the basic structural and functional unit of life. – The cells structural anatomy will determine its overall function. • All cells contains organelles which are the equivalent to organs of an organism. – They provide a specific function within the cell. • produces different types of pro ...
Assembly and function of cell surface structures of the
Assembly and function of cell surface structures of the

... The bas system The bindosome assembly (bas) system is composed of 5 genes, i.e., basEFABC (Zolghadr et al., 2007). The basE gene encodes a cytosolic ATPase while the basF gene specifies a membrane protein. The purification of tagged BasF lead to the co-isolation of BasE demonstrating that these are ...
Protein Digestion by Enzymes
Protein Digestion by Enzymes

... Almost every chemical reaction in the body is catalyzed by a class of proteins called enzymes. There are two main types of enzymes, metabolic and digestive enzymes. The unique amino acid sequence and shape of an enzyme determines its function. Digestive enzymes have a shape that allows it to bind an ...
catalytically active (used for assay)
catalytically active (used for assay)

... loaded on the column at 3 mL/minute on an AKTA-express system (GE/Amersham). The column was then washed with 20 column volumes of lysis buffer, and 10 volumes of wash buffer and then eluted with elution buffer. The eluted peak of A280 was automatically collected. Column 2: Gel Filtration, Hiload 16/ ...
Domains of group A streptococcal M protein that confer resistance to
Domains of group A streptococcal M protein that confer resistance to

... Retnoningrum and Cleary, 1994). The significance of these data rests in the fact that there are more than 150 different M types and, thus, antisera specific to the hypervariable region of one M type will not opsonise other M types. The direction of opsonising antibodies to the hypervariable region o ...
5 nm Ni-NTA-Nanogold
5 nm Ni-NTA-Nanogold

... The His-tag, consisting of five to ten consecutive histidine residues, has been used for purification of proteins by immobilized metalion affinity chromatography (IMAC).2,3 The use of a His tag provides several advantages. There is minimal addition of extra amino acids to the recombinant proteins. T ...
Gene regulation in bacteria -
Gene regulation in bacteria -

... The DNA sequences in promoters before genes that are not continuously active are generally more variable than they are in constitutive promoters, so they are less easily recognised by the RNA polymerase subunit. A gene activator protein helps the RNA polymerase bind to the DNA. The gene activator pr ...
Primary functions Fat-soluble vitamin
Primary functions Fat-soluble vitamin

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Functional inferences from reconstructed evolutionary biology
Functional inferences from reconstructed evolutionary biology

... Homologous proteins have analogous folds. Conversely, nonanalogous folds in two protein families indicate that the two families are not homologous. Thus, if two protein families are predicted to have the same fold, they are more likely to share common ancestry. If two protein families are predicted ...
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ppt - Chair of Computational Biology
ppt - Chair of Computational Biology

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Chapt 5 - Workforce Solutions
Chapt 5 - Workforce Solutions

... • Organisms can maintain osmotic balance in different ways. 1. Some cells use extrusion in which water is ejected through contractile vacuoles Example: Paramecium. 2. Isosmotic regulation involves keeping cells isotonic with their environment Example: Organisms living in oceans. 3. Plant cells use t ...
Growth Factor Pathway - the Biology Scholars Program Wiki
Growth Factor Pathway - the Biology Scholars Program Wiki

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Membrane targeting of proteins
Membrane targeting of proteins

... to the ER for translocation • A protein targets to the ER via a signal sequence, a short stretch of amino acids that is usually at its amino terminus. • The only feature common to all signal sequences is a central, hydrophobic core that is usually sufficient to translocate any associated protein. ...
4.5 Protein Purification Methods
4.5 Protein Purification Methods

... • Protein Folding – The structure and function of a protein depends on protein folding – If protein is folded incorrectly, desired function of a protein is lost and a misfolded protein can be detrimental – 1951 two regular structures were described • Alpha helices and beta sheets • Structures are fr ...
pptx format
pptx format

... Different organisms diverse from each other by the sequence of the basic breaks and their number. ...
CPB- 38- 902
CPB- 38- 902

... Protein Paintbox genes are available in the AOX MeOH-inducible Pichia intracellular vectors to serve as controls. In addition, any Pichiaoptimized Protein Paintbox gene in an Electra MOTHER vector can be cloned into any Electra Pichia DAUGHTER vector. Paintbox genes are not secreted, so we recommend ...
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... 1. Assigning enzymatic activity to a protein. Right click on the first reaction in the pathway, and choose “Edit -> Create/Add Enzyme”. Click the “Search by Genes or Create New Protein” button. Specify “polypeptide” and type in “HP1580” for the gene name. Click OK. The protein Editor opens up. Scrol ...
Transcription part (10/2/2015)
Transcription part (10/2/2015)

... genes. You find a mutation in the transformer gene that results in the female phenotype even in the mutants with a disrupted Sxl gene. What kind of mutations in the Tra gene could have occurred? 16. A researcher introduces a mutation into the AAUAAA sequence in the end of a gene. She finds that a AA ...
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Protein moonlighting



Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.
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