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Organic Molecules Power Point
Organic Molecules Power Point

... Nucleic acids • DNA (Deoxyribonucleic acid) – Genetic material; carries heredity – codes for all proteins ...
Biological Macromolecules
Biological Macromolecules

... (blood sugar), either because insulin production is inadequate, or ...
Chemical Approaches for Quantitative and Functional Proteomics
Chemical Approaches for Quantitative and Functional Proteomics

... – Compare 2 or more samples and find differences in their protein expression • Overlaid on same gel (DIGE) • Compare two or more gels Anal. Chem. 75, 2891-2905. Mass. Spec. Rev. 20, 1-57. Drug Disc. Today 4, 55-62. ...
Structural Genomics - University of Houston
Structural Genomics - University of Houston

... The initial stages of folding must be nearly random, but if the entire process was a random search it would require too much time. Consider a 100 residue protein. If each residue is considered to have just 3 possible conformations the total number of conformations of the protein is 3100. Conformatio ...
Macromolecules of Life
Macromolecules of Life

... Used for support such as connective tissue and keratin that forms hairs and fingernails Transport proteins move many substances through the body Ex. Hemoglobin which transports oxygen through the blood Hormone proteins. Ex, insulin which regulates the amount of sugar in the blood Help control moveme ...
Introduction to Biotechnology Gel Electrophoresis and DNA Analysis
Introduction to Biotechnology Gel Electrophoresis and DNA Analysis

... College of the Canyons: Introduction to Biotechnology Gel Electrophoresis and DNA Analysis: Post Lab 1. What functional group is resposible for DNA’s negative charge? Draw and name it. Phospate: PO4-3 ...
SNUG DC Pamphlet Jan 2007
SNUG DC Pamphlet Jan 2007

... Life as we know it depends on turning on and off the proper genes at the correct time. Gene transcription is how our DNA gets translated into proteins, and ultimately into biological organisms. This work done by the 2006 Chemistry Nobel Prize Laureate Roger Kornberg at the SSRL and ALS synchrotrons ...
Chem of life
Chem of life

... Compound - Two or more different elements chemically combined Ex. H2O, CH4, C6H12O6, NaCl, CH3COOH Mixture - Combination of different compounds and/or elements not chemically combined Molecule - The smallest unit of most compounds Organic - A compound that contains carbon Inorganic - A compound that ...
F9550 - Datasheet - Sigma
F9550 - Datasheet - Sigma

... fragments obtained are denatured and separated on an acrylamide-urea gel, which is then exposed to X-ray film. ...
Document
Document

... indicates function is sequence-specific ...
Antibody
Antibody

... • The purification of proteins is an essential first step in understanding their function. • Purification should yield a sample of protein containing only one type of molecule of interest. • Proteins can be separated from one another on the basis of solubility, size, charge, and binding ability. • A ...
Rhesus ALK-7 / ALK7 / ACVR1C Protein (Fc Tag)
Rhesus ALK-7 / ALK7 / ACVR1C Protein (Fc Tag)

... < 1.0 EU per μg of the protein as determined by the LAL method ...
Dr. Randall H. Goldsmith
Dr. Randall H. Goldsmith

... Single-Molecule measurements offer a wealth of detail about chemical diversity and unsynchronized dynamics, but only if the system under study is conducive to known methods of single-molecule fluoresence microscopy. I will present two cases where new measurement technology enables new observations o ...
IL-3 - CellGenix
IL-3 - CellGenix

... Handling Instructions Reconstitution ...
GelRed™ Product Information Sheet
GelRed™ Product Information Sheet

... GelRed is a sensitive, stable and environmentally safe fluorescent nucleic acid dye designed to replace the highly toxic ethidium bromide (EtBr) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. GelRed and EtBr have virtually the same spectra (Figure 1), so you can directly ...
Examensarbete Activity of carbonic anhydrase II in presence of
Examensarbete Activity of carbonic anhydrase II in presence of

... those aggregates have a specific function, drug delivery, biocatalysis, etc.; unexpected effects can rise from the exposure of those systems to living organism and in particular to the performance of highly functional molecules as enzymes. An important feature of nanomaterials is the protective coat ...
Scholars Research Library Study the modern biochemical analysis
Scholars Research Library Study the modern biochemical analysis

... Peroxidase can be used for treatment of industrial waste water. For examples, phenols, which are important pollutants, can be removed by enzyme – catalized polymerization using horseradish peroxides. Thus phenols are oxidized to phenoxy radicals which participate in reactions where produced polymers ...
PowerPoint 1.5MB - The Biomolecular Modeling & Computational
PowerPoint 1.5MB - The Biomolecular Modeling & Computational

... • Where is the future going? ...
Proteome - Nematode bioinformatics. Analysis tools and data
Proteome - Nematode bioinformatics. Analysis tools and data

... Protein sequence analysis. Bioinformatic branch, search databases for possible protein or peptide matches. Structural proteomics. High-throughput determination of protein structures in three-dimensional space using x-ray crystallography and NMR spectroscopy. Interaction proteomics. Investigation of ...
Chapter 5
Chapter 5

... • Polymers of AA – 20 AA, all varied in their “R” groups – 9 essential AA can not be made by the body ...
Purified Mouse Anti-Human Retinoblastoma
Purified Mouse Anti-Human Retinoblastoma

... Application includes western blot analysis (1-2 µg/ml). Rb migrates as multiple closely-spaced bands between approximately 110-116 kDa when sized on denaturing polyacrylamide gels (i.e. by SDS-PAGE). The different bands represent different Rb phosphorylation states, the higher molecular weight bands ...
Molecular weight determination
Molecular weight determination

... remains after salting-out and dialysis. • To separate protein mixtures on the basis of molecular properties such as size, shape, and weight or certain affinities. • 3 types of chromatographic methods commonly used are: 1. gel-filtration chromatography 2. Ion-exchange chromatography 3. Affinity chrom ...
Proteins = polymers of 20 amino acids, connected by peptide bonds
Proteins = polymers of 20 amino acids, connected by peptide bonds

... Schedule This course is aimed at first and second year graduate students and advanced undergraduates interested in understanding the structures and mechanisms of macromolecular structures in biology. You will learn about the physical basis for these structures, their folding, stability, and interact ...
Laser Light Scattering
Laser Light Scattering

... • The correlation time tc is a measure of the time needed to diffuse a characteristic distance in solution – this distance is defined by the wavelength of light, the scattering angle and the optical properties of the solvent – ranges from 40 to 400 nm in typical systems • Values of tc can range from ...
Analitical chemistry 1
Analitical chemistry 1

... There are two basic types of materials used to make gels: agarose and polyacrylamide. Agarose is very fragile and easily destroyed by handling. Agarose gels have very large "pore" size and are used primarily to separate very large molecules with a molecular mass greater than 200 kdal. Agarose gels c ...
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QPNC-PAGE

QPNC-PAGE, or quantitative preparative native continuous polyacrylamide gel electrophoresis, is a high-resolution technique applied in biochemistry and bioinorganic chemistry to separate proteins by isoelectric point. This standardized variant of native gel electrophoresis is used by biologists to isolate active or native metalloproteins in biological samples and to resolve properly and improperly folded metal cofactor-containing proteins or protein isoforms in complex protein mixtures.
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