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Lecture 4a (1/28/13) "Central Dogma"

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... enzymatic production of D-amino acids have replaced chemical methods. Due to a significant revolution and intensive research in the area of biocatalysis, many biological processes have emerged as great breakthrough in the chirality sciences (3). D-amino acids are utilized in pharmaceuticals, drugs, d ...
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... HYDROXYL GROUP, represented by --OH. An ALCOHOL is an Organic Compound with a Hydroxyl Group attached to one of its Carbon Atoms. The Hydroxyl Group makes Alcohol a Polar molecule that has Some Properties similar to Water, including the Ability to Form Hydrogen Bonds. ...
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... Three types of bonds: ◦ 3) Hydrogen:  Bond that forms between hydrogen atom and the negative atom of another molecules  Ex: between water molecules, connects two strands of DNA, important in protein folding ...
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... The key problems: Oxidative fates of pyruvate – oxidation of pyruvate to acetyl CoA by pyruvate dehydrogenase. The tricarboxylic acid (TCA) cycle (reaction, enzymes, coenzymes, regulation of this cycle). The energetics of the TCA cycle. Cellular bioenergetics: the compounds containing high-energy bo ...
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... Restriction enzymes are part of a bacteria's ''immune'' system. These are enzymes that cut DNA at specific sites (typically a four or a 6 base-pair sequence). Bacterial DNA is modified to be protected by methylation while foreign DNA, such as incoming viruses, are not. Usually, organisms that make ...
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... Chemistry of carbon allows the formation of an enormous variety of organic molecules.  Organic molecules have carbon and hydrogen; determine structure and function of living things.  Inorganic molecules do not contain carbon and hydrogen together; inorganic molecules (e.g., NaCl) can play importan ...
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... Concept 5.4 Proteins perform most functions in cells. (pp. 100–102) A protein is a polymer made from a set of 20 kinds of monomers called amino acids. An amino acid has a central carbon atom bonded to a hydrogen atom, a carboxyl group, an amino group, and a side group. The side group is different fo ...
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...  Long strands of RNA nucleotides that are formed complementary to one strand of DNA Ribosomal RNA (rRNA)  Associates with proteins to form ribosomes in the cytoplasm Transfer RNA (tRNA)  Smaller segments of RNA nucleotides that transport amino acids to the ribosome ...
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... splanchnic circulation rose after a 36- to 48-hour fast but fell markedly after a five- to six-week fast. Furthermore, in prolonged starvation, plasma alanine levels fell to a greater extent than all other amino acids, and the hypoalaninemia, rather than a change in splanchnic fractional extraction ...
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... DNA is a biologically important type of molecule that consists of a long chain of nucleotide units. Each nucleotide consists of a nitrogenous base, a ribose sugar, and a phosphate. RNA is very similar to DNA, but differs in a few important structural details: in the cell, RNA is usually single-stran ...
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... b. Consider the substrate, transition state, and product from the first step of the reaction. Which of these species are stabilized by interactions with the enzyme? Which species is stabilized the most? Briefly explain why. ...
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Biosynthesis



Biosynthesis (also called biogenesis or anabolism) is a multi-step, enzyme-catalyzed process where substrates are converted into more complex products in living organisms. In biosynthesis, simple compounds are modified, converted into other compounds, or joined together to form macromolecules. This process often consists of metabolic pathways. Some of these biosynthetic pathways are located within a single cellular organelle, while others involve enzymes that are located within multiple cellular organelles. Examples of these biosynthetic pathways include the production of lipid membrane components and nucleotides.The prerequisite elements for biosynthesis include: precursor compounds, chemical energy (e.g. ATP), and catalytic enzymes which may require coenzymes (e.g.NADH, NADPH). These elements create monomers, the building blocks for macromolecules. Some important biological macromolecules include: proteins, which are composed of amino acid monomers joined via peptide bonds, and DNA molecules, which are composed of nucleotides joined via phosphodiester bonds.
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