Experiment 1 Comparison between Enzymes and non
... The substrate sucrose is a non-reducing sugar, where as the products formed are both reducing sugar. Therefore the reaction can be followed by the estimation of the quantity of reducing sugar formed. Between the several methods which can be used for such estimation, Benedict quantitative method was ...
... The substrate sucrose is a non-reducing sugar, where as the products formed are both reducing sugar. Therefore the reaction can be followed by the estimation of the quantity of reducing sugar formed. Between the several methods which can be used for such estimation, Benedict quantitative method was ...
A novel approach for protein subcellular location prediction using
... the Lsg1 family of GTPases [9] or locations taken as known might be predicted or incorrect. A third approach uses the amino acid composition of the protein as a proxy for location based on the hypothesis that the physicochemical properties of the residues of a protein must be somehow coupled to the ...
... the Lsg1 family of GTPases [9] or locations taken as known might be predicted or incorrect. A third approach uses the amino acid composition of the protein as a proxy for location based on the hypothesis that the physicochemical properties of the residues of a protein must be somehow coupled to the ...
ENZYME WEBQUEST Name
... 18. The conformation of an enzyme is maintained by interactions between the various ___________________ _______________ that compose it, and this conformation is sensitive to ________________________________________. Two important influences are _________________ and ________________. When an enzyme ...
... 18. The conformation of an enzyme is maintained by interactions between the various ___________________ _______________ that compose it, and this conformation is sensitive to ________________________________________. Two important influences are _________________ and ________________. When an enzyme ...
Tps1 regulates the pentose phosphate pathway, nitrogen
... produced from 10 mM nitrate in the presence of cell protein extract was reacted with sulphanilamide and (1-Naphthyl) ethylenediamine dihydrochloride to form a nitrite coloured complex which could be quantified at A540nm. The reaction was performed in the presence of 2.5 mM sodium sulphate to inhibit ...
... produced from 10 mM nitrate in the presence of cell protein extract was reacted with sulphanilamide and (1-Naphthyl) ethylenediamine dihydrochloride to form a nitrite coloured complex which could be quantified at A540nm. The reaction was performed in the presence of 2.5 mM sodium sulphate to inhibit ...
Isoleucine Synthesis by Clostridium sporogenes from
... with 0.02 M-sodium citrate buffer, pH 2.99, containing 3% (v/v) n-propanol was used to separate aspartate, threonine, serine, glutamate, glycine, and alanine. Program I1 with 0.2 M-sodium citrate buffer, pH 3.39, containing 3 % (v/v) methanol was used to separate valine, methionine, isoleucine, and ...
... with 0.02 M-sodium citrate buffer, pH 2.99, containing 3% (v/v) n-propanol was used to separate aspartate, threonine, serine, glutamate, glycine, and alanine. Program I1 with 0.2 M-sodium citrate buffer, pH 3.39, containing 3 % (v/v) methanol was used to separate valine, methionine, isoleucine, and ...
Chapter 5 - Biology Junction
... Concept 5.3: Lipids are a diverse group of hydrophobic molecules • Lipids are the one class of large biological molecules that do not form polymers • The unifying feature of lipids is having little or no affinity for water • Lipids are hydrophobic because they consist mostly of hydrocarbons, which ...
... Concept 5.3: Lipids are a diverse group of hydrophobic molecules • Lipids are the one class of large biological molecules that do not form polymers • The unifying feature of lipids is having little or no affinity for water • Lipids are hydrophobic because they consist mostly of hydrocarbons, which ...
Chapter 18 Glycolysis
... Hanjia’s Biochemistry Lecture Figure 18.19 The conversion of phosphoenolpyruvate (PEP) to pyruvate may be viewed as involving two steps: phosphoryl transfer followed by an enol-keto tautomerization. The tautomerization is spontaneous (GƱ' = -35-40 kJ/mol) and accounts for much of the free energy ch ...
... Hanjia’s Biochemistry Lecture Figure 18.19 The conversion of phosphoenolpyruvate (PEP) to pyruvate may be viewed as involving two steps: phosphoryl transfer followed by an enol-keto tautomerization. The tautomerization is spontaneous (GƱ' = -35-40 kJ/mol) and accounts for much of the free energy ch ...
Calvin Cycle Flux, Pathway Constraints, and
... ABSTRACT Hydrogen gas (H2) is a possible future transportation fuel that can be produced by anoxygenic phototrophic bacteria via nitrogenase. The electrons for H2 are usually derived from organic compounds. Thus, one would expect more H2 to be produced when anoxygenic phototrophs are supplied with i ...
... ABSTRACT Hydrogen gas (H2) is a possible future transportation fuel that can be produced by anoxygenic phototrophic bacteria via nitrogenase. The electrons for H2 are usually derived from organic compounds. Thus, one would expect more H2 to be produced when anoxygenic phototrophs are supplied with i ...
ENZYME STUDIES ON CHROMOSOMES [ 40 ]
... enzyme of frog liver was effective will be explained only when its activity against artificial substrates will have been studied. This information might give an important clue concerning the nature of the linkages responsible for the chromosome structure. Tumor enzyme. Since dividing cells should be ...
... enzyme of frog liver was effective will be explained only when its activity against artificial substrates will have been studied. This information might give an important clue concerning the nature of the linkages responsible for the chromosome structure. Tumor enzyme. Since dividing cells should be ...
enzyme activity
... cannot digest milk sugar (lactose); however, they can take supplements containing lactase, the enzyme they are missing. All of these enzymes hydrolyze large, complex molecules into their simpler components; bromelain and papain break proteins down to amino acids, while lactase breaks lactose down to ...
... cannot digest milk sugar (lactose); however, they can take supplements containing lactase, the enzyme they are missing. All of these enzymes hydrolyze large, complex molecules into their simpler components; bromelain and papain break proteins down to amino acids, while lactase breaks lactose down to ...
222 18.3 Oxidation and Flour Maturation
... lipoxygenase, peroxides are formed that have a cross-linking effect on thiol groups. However, the gluten-strengthening effect of soy flour is comparatively slight; its bleaching effect is more important. There are several types of lipoxygenases with different action patterns. While type I lipoxygena ...
... lipoxygenase, peroxides are formed that have a cross-linking effect on thiol groups. However, the gluten-strengthening effect of soy flour is comparatively slight; its bleaching effect is more important. There are several types of lipoxygenases with different action patterns. While type I lipoxygena ...
Chapter 5
... Concept 5.3: Lipids are a diverse group of hydrophobic molecules • Lipids are the one class of large biological molecules that do not form polymers • The unifying feature of lipids is having little or no affinity for water • Lipids are hydrophobic because they consist mostly of hydrocarbons, which ...
... Concept 5.3: Lipids are a diverse group of hydrophobic molecules • Lipids are the one class of large biological molecules that do not form polymers • The unifying feature of lipids is having little or no affinity for water • Lipids are hydrophobic because they consist mostly of hydrocarbons, which ...
Copyright Information of the Article Published Online
... This means that the other proteins present in the mitochondria (i.e., around 2000 proteins) are the products of nuclear genes. As for any nuclear genes, the corresponding proteins are synthesized in the cytosol, although mitochondrial proteins are subsequently imported into the mitochondria. The imp ...
... This means that the other proteins present in the mitochondria (i.e., around 2000 proteins) are the products of nuclear genes. As for any nuclear genes, the corresponding proteins are synthesized in the cytosol, although mitochondrial proteins are subsequently imported into the mitochondria. The imp ...
It Works! Shake™ Product Info Sheet
... free of antibiotics and growth hormones. Why do I need It Works! Shake? Because you need protein! There are nine essential amino acids that your body cannot make and you can get them from a high protein diet. These amino acids are necessary for everything from immune function, metabolism and weight ...
... free of antibiotics and growth hormones. Why do I need It Works! Shake? Because you need protein! There are nine essential amino acids that your body cannot make and you can get them from a high protein diet. These amino acids are necessary for everything from immune function, metabolism and weight ...
The Role of the Krebs Cycle in Conjugation in
... cycle for a C, fragment which would stimulate the oxidation of C, fragments (derived from glucose) via the tricarboxylic acid cycle. The demonstration in Escherichia coli of the reversible reaction aspartic acid+fumaric acid ammonia (Quastel& Woolf, 1926),as well as the possession by this organism o ...
... cycle for a C, fragment which would stimulate the oxidation of C, fragments (derived from glucose) via the tricarboxylic acid cycle. The demonstration in Escherichia coli of the reversible reaction aspartic acid+fumaric acid ammonia (Quastel& Woolf, 1926),as well as the possession by this organism o ...
Powerpoint Presentation: Translation
... Frame shift mutations can be caused by the ADDITION or DELETION of only one or two bases. Everything downstream is misread ...
... Frame shift mutations can be caused by the ADDITION or DELETION of only one or two bases. Everything downstream is misread ...
11 Cytochrome P450 and the Metabolism and Bioactivation of
... metabolic cascade, P450 metabolizes only free, nonesterified forms of AA and thus, in vivo metabolism requires the release of the fatty acid from selected glycerophospholipid pools. CYP P450, prostaglandin H2 synthase, and lipoxygenases are capable of metabolizing polyunsaturated fatty acids other t ...
... metabolic cascade, P450 metabolizes only free, nonesterified forms of AA and thus, in vivo metabolism requires the release of the fatty acid from selected glycerophospholipid pools. CYP P450, prostaglandin H2 synthase, and lipoxygenases are capable of metabolizing polyunsaturated fatty acids other t ...
Arterial Blood Gas Interpretation: The Basics
... In the presence of respiratory acidosis the kidneys compensate for the fall in pH by excreting H+ ions and retaining HCO3- ions. As a result, pH rises towards normal and HCO3concentration rises above normal. Renal compensation (also called metabolic compensation) to respiratory acidosis is a slow pr ...
... In the presence of respiratory acidosis the kidneys compensate for the fall in pH by excreting H+ ions and retaining HCO3- ions. As a result, pH rises towards normal and HCO3concentration rises above normal. Renal compensation (also called metabolic compensation) to respiratory acidosis is a slow pr ...
Phosphorus-31 nuclear magnetic resonance studies of intracellular
... cytoplasmic phosphate from its response to glucose addition and iodoacetate inhibition of glycolysis. After addition of substrate the pH in the compartment containing the second phosphate pool decreased. A parallel response was observed for a significant fraction of the terminal and penultimate phos ...
... cytoplasmic phosphate from its response to glucose addition and iodoacetate inhibition of glycolysis. After addition of substrate the pH in the compartment containing the second phosphate pool decreased. A parallel response was observed for a significant fraction of the terminal and penultimate phos ...
The Role of the Krebs Cycle in Conjugation in
... cycle for a C, fragment which would stimulate the oxidation of C, fragments (derived from glucose) via the tricarboxylic acid cycle. The demonstration in Escherichia coli of the reversible reaction aspartic acid+fumaric acid ammonia (Quastel& Woolf, 1926),as well as the possession by this organism o ...
... cycle for a C, fragment which would stimulate the oxidation of C, fragments (derived from glucose) via the tricarboxylic acid cycle. The demonstration in Escherichia coli of the reversible reaction aspartic acid+fumaric acid ammonia (Quastel& Woolf, 1926),as well as the possession by this organism o ...
ATP Pool and Growth Yield in Selenomonas
... by formation of storage polysaccharide, which is essentially a 'high-yield' process. There remain, however, some yields which, on the basis of known or suggested substrate-level production of ATP, are apparently nearer 20 than 10. This was first noted with some strictly anaerobic rumen bacteria (Hob ...
... by formation of storage polysaccharide, which is essentially a 'high-yield' process. There remain, however, some yields which, on the basis of known or suggested substrate-level production of ATP, are apparently nearer 20 than 10. This was first noted with some strictly anaerobic rumen bacteria (Hob ...
Oxidative Phosphorylation accompanying Oxidation of
... that serum albumin, or its bound fatty acids, acted as a substrate in the absence of butyrate but was not a good precursor of ketone bodies. As the rate of oxidation of butyrate was inconveniently low in the absence of serum albumin, this protein was Fig. 3. Effect of hexokinase on the effective ade ...
... that serum albumin, or its bound fatty acids, acted as a substrate in the absence of butyrate but was not a good precursor of ketone bodies. As the rate of oxidation of butyrate was inconveniently low in the absence of serum albumin, this protein was Fig. 3. Effect of hexokinase on the effective ade ...
ATP Pool and Growth Yield in Selenomonas
... by formation of storage polysaccharide, which is essentially a 'high-yield' process. There remain, however, some yields which, on the basis of known or suggested substrate-level production of ATP, are apparently nearer 20 than 10. This was first noted with some strictly anaerobic rumen bacteria (Hob ...
... by formation of storage polysaccharide, which is essentially a 'high-yield' process. There remain, however, some yields which, on the basis of known or suggested substrate-level production of ATP, are apparently nearer 20 than 10. This was first noted with some strictly anaerobic rumen bacteria (Hob ...
Reduced lipid intake leads to changes in - Archimer
... growth but liver fatty acid metabolism as well as muscle n-3 PUFA contents are affected in Atlantic salmon (Salmo salar) (Torstensen et al., 2000; Bell et al., 2001 and 2002; Stubhaug et al., 2005). However, using a fish oil ‘finishing diet’ at the end of the experiment can partially restore the EPA ...
... growth but liver fatty acid metabolism as well as muscle n-3 PUFA contents are affected in Atlantic salmon (Salmo salar) (Torstensen et al., 2000; Bell et al., 2001 and 2002; Stubhaug et al., 2005). However, using a fish oil ‘finishing diet’ at the end of the experiment can partially restore the EPA ...
THE LIVER AS AN ORGAN
... immunoglobulins, binding proteins, cholesterol, lipoproteins, bile, and other important molecules. Cholesterol production and excretion: The body requires cholesterol, and although this substance can be synthesized by many cells in the body, the liver can produce it at a high rate when necessary (wi ...
... immunoglobulins, binding proteins, cholesterol, lipoproteins, bile, and other important molecules. Cholesterol production and excretion: The body requires cholesterol, and although this substance can be synthesized by many cells in the body, the liver can produce it at a high rate when necessary (wi ...
Metabolism
Metabolism (from Greek: μεταβολή metabolē, ""change"") is the set of life-sustaining chemical transformations within the cells of living organisms. These enzyme-catalyzed reactions allow organisms to grow and reproduce, maintain their structures, and respond to their environments. The word metabolism can also refer to all chemical reactions that occur in living organisms, including digestion and the transport of substances into and between different cells, in which case the set of reactions within the cells is called intermediary metabolism or intermediate metabolism.Metabolism is usually divided into two categories: catabolism, the breaking down of organic matter by way of cellular respiration, and anabolism, the building up of components of cells such as proteins and nucleic acids. Usually, breaking down releases energy and building up consumes energy.The chemical reactions of metabolism are organized into metabolic pathways, in which one chemical is transformed through a series of steps into another chemical, by a sequence of enzymes. Enzymes are crucial to metabolism because they allow organisms to drive desirable reactions that require energy that will not occur by themselves, by coupling them to spontaneous reactions that release energy. Enzymes act as catalysts that allow the reactions to proceed more rapidly. Enzymes also allow the regulation of metabolic pathways in response to changes in the cell's environment or to signals from other cells.The metabolic system of a particular organism determines which substances it will find nutritious and which poisonous. For example, some prokaryotes use hydrogen sulfide as a nutrient, yet this gas is poisonous to animals. The speed of metabolism, the metabolic rate, influences how much food an organism will require, and also affects how it is able to obtain that food.A striking feature of metabolism is the similarity of the basic metabolic pathways and components between even vastly different species. For example, the set of carboxylic acids that are best known as the intermediates in the citric acid cycle are present in all known organisms, being found in species as diverse as the unicellular bacterium Escherichia coli and huge multicellular organisms like elephants. These striking similarities in metabolic pathways are likely due to their early appearance in evolutionary history, and their retention because of their efficacy.