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Gene overexpression reveals alternative
Gene overexpression reveals alternative

... increase in GCN4 mRNA translation, coupled with an extensive protein synthesis shut down. However, both phenomena are independent of Gcn2 function (Tzamarias et al., 1989). In addition, the assimilation of GCN4 mRNA translational derepression in vitro, does not require the Gcn2 kinase (Krupitza and ...
Agilent Whole Human Genome Oligo Microarray Kit
Agilent Whole Human Genome Oligo Microarray Kit

... 3 different consensus regions were chosen, and probes selected from those consensus regions (shown in the bottom section). Clustering data in Figure B demonstrates that probes for consensus regions 2 and 3 exhibit similar behavior, while probes for consensus region 1 exhibit different behavior, and ...
Shastry, B.S. 2002. SNP alleles in human disease and evolution
Shastry, B.S. 2002. SNP alleles in human disease and evolution

... for evolutionary biology studies, gene discovery and mapping, prediction of drug and environmental response, diagnostic tests, heterogeneity testing, and association studies (Gray et al. 2000; Schork et al. 2000). For the purpose of gene discovery, SNPs are considered to be the most predominant segr ...
ABSTRACT Using a bioinformatics approach to identify genes that
ABSTRACT Using a bioinformatics approach to identify genes that

... that even though there are numerous genes thought to be associated with RP, there is no singular causative agent. This may also imply that there are other mechanisms or genes associated with RP that have yet to be identified. Automated or semi-automated bioinformatics techniques can be used to compa ...
extensions
extensions

... in a recessive fashion ...
E1. Mexican hairless dogs are heterozygous for a dominant allele
E1. Mexican hairless dogs are heterozygous for a dominant allele

... E1. Mexican hairless dogs are heterozygous for a dominant allele that is lethal when homozygous. In a cross between two Mexican hairless dogs, we expect 1/4 to be normal, 1/2 to be hairless, and 1/4 to die. E2. Chinchilla 1 is heterozygous c chc. Chinchilla 2 is heterozygous c chc h. Chinchilla 3 is ...
extensions
extensions

... frequency in parts of Africa where malaria is found • Malaria is caused by a protozoan, Plasmodium – This parasite undergoes its life cycle in two main parts • One inside the Anopheles mosquito • The other inside red blood cells ...
Bacterial conjugation
Bacterial conjugation

... other words, physical contact between the two strains was needed for wild-type cells to form. It looked as though some kind of gene transfer had taken place, ...
Document
Document

... Overall, the results of these two crosses indicate that the purple allele is autosomal, and the vermilion allele is X linked. To confirm this idea, the vermilion males could be crossed to homozygous wild-type females. The F1 females should have red eyes. If these F1 females are crossed to wild-type ...
Recent and ongoing selection in the human genome
Recent and ongoing selection in the human genome

... Genome-wide scans for sweeps in humans The LCT and G6PD loci provide some of the most striking examples of ongoing selective sweeps in the human genome. These genes were identified a priori as candidate genes on the basis of functional information. Several recent papers have aimed at detecting loci ...
Reliable classification of two-class cancer data using evolutionary
Reliable classification of two-class cancer data using evolutionary

... be made to generalize the procedure for any number of classes. In most problems involving identification of gene subsets in bioinformatics, the number of samples available is small when compared to the gene pool size. This aspect makes it difficult to identify which and how many genes are correlated ...
1 Sequential elimination of major-effect contributors
1 Sequential elimination of major-effect contributors

... confounding effects of major loci and epistasis. Therefore, to avoid confounding effects of major loci, epistasis and sample size, we used a targeted backcross mapping strategy that genetically eliminated the effect of a previously identified major QTL underlying high-temperature growth (Htg) in yea ...
Linkage disequilibrium mapping in trisomic populations: analytical approaches and an application to congenital heart defects in Down syndrome.
Linkage disequilibrium mapping in trisomic populations: analytical approaches and an application to congenital heart defects in Down syndrome.

... variable phenotype in question would show greater than expected levels of disomic homozygosity in the genomic region containing the susceptibility gene. The limitation of this linkage approach is that it cannot test genetic models that do not lead to increased disomic homozygosity (e.g., those with ...
Supplementary Methods, Figure Legends, Table
Supplementary Methods, Figure Legends, Table

... TNBCs from the PrECOG dataset according to SAbCNA, SAiCNA and SCnLOH. The three TNBC clusters identified include Lo-SCINS (green arm of dendrogram), Hi-AiCNA (red arm of dendogram) and Hi-CnLOH (blue arm of dendrogram). Scores were standardised by row mean and a colour scale is shown to the left. La ...
XSL Formatter - H:\XML
XSL Formatter - H:\XML

... Dombrowski, and Andrei Gabrelian ...
Drosophila melanogaster Preferential Segregation of the Fourth
Drosophila melanogaster Preferential Segregation of the Fourth

... Other chromosomes are'being studied, including two crossover ones. These should throw light on the mechanism concerned, but are not yet ready to report on. An unexpected result is that these preferences are very much reduced in the male, though apparently still present. In the two best-studied cases ...
Series 1: Cross Diagrams There are two alleles for each trait in a
Series 1: Cross Diagrams There are two alleles for each trait in a

... dpy unc dpy unc ...
Interaction of a GATA factor with cis-acting elements involved in light
Interaction of a GATA factor with cis-acting elements involved in light

... We have previously identified a cis-acting element, named the XXIII box, that is essential for light-regulated expression of the nuclear gene GAPB, which encodes the B subunit of chloroplast glyceraldehyde-3-phosphate dehydrogenase from Arabidopsis thaliana. Examination of the sequences indicated tha ...
Genetic Differences in Endothelial Cells May Determine
Genetic Differences in Endothelial Cells May Determine

... lesions add to the difficulty of isolating genes for atherosclerosis susceptibility using this model. Thus, it is clear that new approaches will be needed with this model to identify the genes responsible for the differences observed in susceptibility to atherosclerosis. The article in the current i ...
Genetics and Biotechnology
Genetics and Biotechnology

...  The newly generated DNA molecule with DNA from different sources is called recombinant DNA. ...
Invited Review: How sleep deprivation affects gene expression in
Invited Review: How sleep deprivation affects gene expression in

... Our laboratory employs mRNA differential display and cDNA microarray technology to systematically establish the differences in gene expression that occur across behavioral states. We compared brain gene expression after short (3 h) and sustained (8 h) periods of sleep, spontaneous wakefulness, and s ...
studies on human x-chromosomal disorder
studies on human x-chromosomal disorder

... incurable, till today) have been found to have their origins on the X chromosome. Although there are numerous X-linked disorders, few very important diseases were considered to be analyzed using a bioinformatics approach. The discovery may throw some light on various findings which could be very use ...
Evolution of meiosis genes in sexual vs. asexual Potamopyrgus
Evolution of meiosis genes in sexual vs. asexual Potamopyrgus

... sexual reproduction should have little or no functional relevance for asexuals, I can use this approach to study the effects of asexuality on the evolution of genes required only for sexual reproduction, which has implications for genome evolution in the absence of sex. I discovered that genes neces ...
Positional dependence of transcriptional inhibition by DNA torsional
Positional dependence of transcriptional inhibition by DNA torsional

... became the predominant over-represented category (Supplementary Table I). Physical clustering of altered genes on accumulation of DNA helical stress To examine how transcriptome alterations between the above top2ts and TOP2 strains spread throughout the yeast chromosomes after the accumulation of DN ...
Physical map of the aromatic amine and m-toluate
Physical map of the aromatic amine and m-toluate

... appears to form part of a unique meta-cleavage pathway for the degradation of rn-toluate, benzoate and aromatic amines. Additionally, we demonstrate that pTDNl did not arise from the chromosomeof strain mt-2 and is in no way derived from pWW0; we speculate on the origin of this plasmid. ...
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Minimal genome

The concept of minimal genome assumes that genomes can be reduced to a bare minimum, given that they contain many non-essential genes of limited or situational importance to the organism. Therefore, if a collection of all the essential genes were put together, a minimum genome could be created artificially in a stable environment. By adding more genes, the creation of an organism of desired properties is possible. The concept of minimal genome arose from the observations that many genes do not appear to be necessary for survival. In order to create a new organism a scientist must determine the minimal set of genes required for metabolism and replication. This can be achieved by experimental and computational analysis of the biochemical pathways needed to carry out basic metabolism and reproduction. A good model for a minimal genome is Mycoplasma genitalium, the organism with the smallest known genome. Most genes that are used by this organism are usually considered essential for survival; based on this concept a minimal set of 256 genes has been proposed.
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