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Metatranscriptomic analysis of the Gut microbial community
Metatranscriptomic analysis of the Gut microbial community

...  Pinpoint specific genes and pathways that are influenced by microbial gene expression  Dissect the influence of diet and microbiome  Obese microbiota on high fat/ high carb diet will gain more than lean microbiota on High fat/ high carb diet  Down-regulation of host adipogenic genes under above ...
Supporting Information (SI) for “Theoretical models of the influence
Supporting Information (SI) for “Theoretical models of the influence

... these are absent in the “beanbag” scenario, where dynamics of alleles at different loci are completely independent of one another because offspring are produced from a population “beanbag”). The “genome only” scenario has genes organized in genomes and gametes are produced from individuals by meiosi ...
4 Conjugation in E. coli
4 Conjugation in E. coli

... E. coli’s chromosome is one continuous DNA-molecule, about 1.3 mm on length. In the cytoplasm of some E. coli-strains, is a so-called F-factor which is a small circular DNA molecule which goes under replication independent to the chromosome’s replication. The Bacteria which have the F-factor are cal ...
CONTROL OF THE ACTIVITY OF THE HUMAN MITOCHONDRIAL TRANSCRIPTION TERMINATION FACTOR
CONTROL OF THE ACTIVITY OF THE HUMAN MITOCHONDRIAL TRANSCRIPTION TERMINATION FACTOR

... Mitochondria are nearly ubiquitous organelles within eukaryotic cells. Only four ...
The landscape of microbial phenotypic traits and associated genes
The landscape of microbial phenotypic traits and associated genes

... ecosystem type. For instance, organisms annotated as ‘marine’ were used as provisional negatives for ‘soil’ or for ’thermal springs’. In GOLD, each organism can have more than one assigned value, e.g. being annotated as both ‘marine’ and ‘freshwater’ and thus receiving positive labels for these two ...
Reverse Genetic Analysis of Terminal Ear
Reverse Genetic Analysis of Terminal Ear

... Table 9: Primer sequences used for the amplification of either the wild type genomic DNA sequence or the T-DNA insert sequence from the target gene ................... 50 Table 10: Expected product sizes for the amplified TEL1 and TEL2 alleles used in this research to create the tel1tel2 double muta ...
Eukaryotic Chromosome Mapping
Eukaryotic Chromosome Mapping

... Using a Three-point Testcross to Determine Genetic Distance • A cross between two parental strains is used to produce a tri-hybrid (heterozygous for three genes). • The tri-hybrid is crossed to an organism that is homozygous recessive for all three genes. • Eight classes of offspring are analyzed t ...
Meiosis - My Haiku
Meiosis - My Haiku

... All living things have thousands of genes. Genes determine individual traits. The genes do not just float around in a cell. They are lined up on chromosomes. A typical chromosome can have thousands of genes. If you took a cell from one of Mendel’s pea plants, you would see that it has 14 chromosomes ...
IGV Handout file
IGV Handout file

... and analyzing large genome datasets. It is a lightweight genomic data viewer on which you can work with prebuilt genomes or load any genome that you want. It may be used for viewing a variety of data such as expression data, NGS alignments, microarray, epigenomics, RNA-Seq, genomic annotations etc. ...
Chapter 12
Chapter 12

... From E.Coli to a Map of Our Genes • Research on E. coli revealed that these bacteria have a sexual mechanism that can bring about the combining of genes from two different cells • This discovery led to the development of recombinant DNA technology – a set of techniques for combining genes from diff ...
Molecular analysis of the operon which encodes the
Molecular analysis of the operon which encodes the

... restriction enzymes. Southern blotting showed that none gave a single hybridization-positive band, but digestion with BgAI produced two positive bands, of 4 and 6 kb. These fragments were cloned into the BamHI site of pUC18 to give p R P 0 4 and pRPO6 respectively. In this way large flanking regions ...
Sample Chapter - McGraw Hill Higher Education
Sample Chapter - McGraw Hill Higher Education

... Structure of DNA Chromosomes are composed of chromatin, which is DNA and associated proteins. A DNA molecule is a simple, elegant chain of building blocks called nucleotides. Each nucleotide consists of three parts: (1) a nitrogen-containing compound, called a base; (2) a 5-carbon sugar, named deoxy ...
Adaptive Evolution of 5#HoxD Genes in the
Adaptive Evolution of 5#HoxD Genes in the

... *School of Life Science, East China Normal University, Shanghai, China;  Guangdong Entomological Institute, Guangzhou, China; àSchool of Biological and Chemical Sciences, Queen Mary, University of London, London, United Kingdom; §Institute of Zoology, Chinese Academy of Sciences, Beijing, China; kSc ...
deschamp_2009_sequencing
deschamp_2009_sequencing

... Polymorphism rate in one line vs. another = need to set conditions for alignment ...
View/print full test page
View/print full test page

... Christodoulou, John, and Gladys Ho. “MECP2-Related Disorders.” In GeneReviewsTM, edited by Roberta A Pagon, Margaret P Adam, Thomas D Bird, Cynthia R Dolan, Chin-To Fong, and Karen Stephens. Seattle (WA): University of Washington, Seattle, 1993. http://www.ncbi.nlm.nih.gov/books/NBK1497/. Dagli, Adi ...
Outline 1. Zen of Screen vs Selection 2. Mutation Rate
Outline 1. Zen of Screen vs Selection 2. Mutation Rate

... The tools developed in the early days of bacterial and phage genetics have allowed the near complete genetic dissection of some very important pathogens. ...
How mammalian sex chromosomes acquired their peculiar gene
How mammalian sex chromosomes acquired their peculiar gene

... studied was those involved in sex and reproduction. Using publicly available data, it was noted that a greater proportion of sex- and reproduction-related traits mapped to the human X chromosome than to autosomes. Of 141 disease-related loci on the X chromosome, 46 are related to sex or reproduction ...
No Slide Title
No Slide Title

... chromatin fibers. • Despite over 2 decades of investigation the structure of the “30nm” chromatin fiber is not known. • This may be due to irregularity or instability of the structure. • This level of structure has been implicated in mechanisms of chromatin repression, thus, the lack of structural i ...
ppt
ppt

... gene. So, often (especially if k is small) it will lead to no change at all. But that’s not a problem – in the EA context, it means that the next generation contains an extra copy of an individual that survived selection (so is probably quite good), and in fact it might not be in the new population ...
Chromatin Signature Identifies Monoallelic Gene Expression Across
Chromatin Signature Identifies Monoallelic Gene Expression Across

... different ChIP-Seq datasets. To note, in this context, the use of quantile rank is equivalent to quantile normalization, but without requiring a reference dataset. Consequently, the newly trained classifier can be readily applied to other datasets with gene2body signal expressed as quantile-rank, eve ...
Appendix_1_SimpleNomenclature(plain)
Appendix_1_SimpleNomenclature(plain)

... region of prokaryotes. They are transcribed into an RNA message by RNA polymerase then interpreted by ribosomes that assemble particular amino acids into a polypeptide strand (also known as a protein) based on the sequence of nucleotides. In a cell, proteins can act as enzymes, structural features, ...
The UCSC Genome Browser
The UCSC Genome Browser

... choose a mouse assembly in the Genome Browser Gateway and enter ADAM2 in the position field, just as we did for human. However, this approach is risky, since orthologs do not always have the same names. In this case, it turns out that the intuitive approach gives you a clue as to where the mouse ort ...
Chapt 16: Other RNA Processing 16.1 Ribosomal RNA Processing
Chapt 16: Other RNA Processing 16.1 Ribosomal RNA Processing

... • mRNA in eukaryotes frequently requires splicing, but does not undergo any trimming from ends • rRNA genes of both eukaryotes and bacteria are transcribed as larger precursors; must be processed to yield rRNAs of mature size • Several different rRNA molecules are embedded in a long, precursor; each ...
Non-Mendelian Genetics
Non-Mendelian Genetics

... B.Chemical basis of traits 1. DNA is the molecule that contains the information to make proteins, which control our traits. 2. A section of DNA that is used to make a protein is called a gene. There are many genes (hundreds) on a single chromosome. 3. Eukaryotic organism’s chromosomes exist in pair ...
Transformation Lab
Transformation Lab

... transformed into plants. In bioremediation, bacteria can be genetically transformed with genes enabling them to digest oil spills. In medicine, diseases caused by defective genes are beginning to be treated by gene therapy; that is, by genetically transforming a sick person’s cells with healthy copi ...
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Minimal genome

The concept of minimal genome assumes that genomes can be reduced to a bare minimum, given that they contain many non-essential genes of limited or situational importance to the organism. Therefore, if a collection of all the essential genes were put together, a minimum genome could be created artificially in a stable environment. By adding more genes, the creation of an organism of desired properties is possible. The concept of minimal genome arose from the observations that many genes do not appear to be necessary for survival. In order to create a new organism a scientist must determine the minimal set of genes required for metabolism and replication. This can be achieved by experimental and computational analysis of the biochemical pathways needed to carry out basic metabolism and reproduction. A good model for a minimal genome is Mycoplasma genitalium, the organism with the smallest known genome. Most genes that are used by this organism are usually considered essential for survival; based on this concept a minimal set of 256 genes has been proposed.
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