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Transcriptional Induction of Genes Encoding ER Resident Proteins
Transcriptional Induction of Genes Encoding ER Resident Proteins

... • UPRE in KAR2 promoter can function as UAS when fused to a heterologous promoter. •UPRE from KAR2 was inserted upstream of a crippled CYC1 promoter that is transcriptionally silent in the absence of UAS. •Single copies of reporter construct were integrated at two different locations to create JC103 ...
Marker Gene Technologies, Inc
Marker Gene Technologies, Inc

... buyer (whether the buyer is an academic or for-profit entity). No license is conveyed under the foregoing patents to use this product with any recombination sites other than those purchased from Marker Gene Technologies or its authorized distributor. The buyer cannot modify the recombination sequenc ...
The osmZ
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... of a 10 mg/ml solution of 5-bromo-4-chloro-3-indolyl-flD-galactoside (XG) in dimethylformamide were used to distinguish between LacZ + and LacZ- phage plaques and colonies. Antibiotics were used at the following concentrations: kanamycin (Kin), 30 rag/l; ampicillin (Ap), 50 rag/l; tetracycline (Tc), ...
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The making of the Fittest: Natural Selection and Adaptation
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Chapter 12 Review - Baldwinsville Central School District
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asexual seed formation for agricultural crop improvement
asexual seed formation for agricultural crop improvement

... sequences involved in key biological processes in both plants and animals were implemented and queried to better annotate our sequence sets. The analysis of sequence composition of each library enabled the identification of transcripts that are likely exclusive of single flower parts and reproductiv ...
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genetic drift

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... a) The Hardy-Weinberg theorem describes a hypothetical population that is not evolving b) The 5 conditions for non-evolving populations are rarely met in nature i) No mutations ii) Random mating iii) no natural selection iv) Very large population size v) no gene flow c) In real population these 5 fa ...
The Big Picture: an outline of the concepts covered to date
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Mrs. Sevgi
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... purebred pea plants. Use the trait pea color for your description. B. Mendelian Laws Through Mendel’s many years and hundreds of pieces data, he threw out many old ideas about heredity and came up with four hypotheses that turned into two laws that still hold true today. The first hypothesis stated ...
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Title, arial 30pt Bold, all caps

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OCR GCSE (9-1) Gateway Science Biology A
OCR GCSE (9-1) Gateway Science Biology A

... 3. Introduce the individual shoes as chromosomes one from mother (lift up the female shoe) one from the father (lift up the male shoe). Talk about the position of genes (the locus). Point at a position of one of the shoes and say that this is the position of the eye colour gene. Ask the learners whe ...
Biological Annotation in R
Biological Annotation in R

... •Allows for the annotation and analysis of function simply and easily •Most array types are catered for •Species specific data also exist (most model species) •Even if the database doesn’t exist your species, but is present in the ncbi repositories >library(AnnotationForge) ...
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Genetic engineering



Genetic engineering, also called genetic modification, is the direct manipulation of an organism's genome using biotechnology. It is therefore a set of technologies used to change the genetic makeup of cells, including the transfer of genes within and across species boundaries to produce improved or novel organisms. New DNA may be inserted in the host genome by first isolating and copying the genetic material of interest using molecular cloning methods to generate a DNA sequence, or by synthesizing the DNA, and then inserting this construct into the host organism. Genes may be removed, or ""knocked out"", using a nuclease. Gene targeting is a different technique that uses homologous recombination to change an endogenous gene, and can be used to delete a gene, remove exons, add a gene, or introduce point mutations.An organism that is generated through genetic engineering is considered to be a genetically modified organism (GMO). The first GMOs were bacteria generated in 1973 and GM mice in 1974. Insulin-producing bacteria were commercialized in 1982 and genetically modified food has been sold since 1994. Glofish, the first GMO designed as a pet, was first sold in the United States December in 2003.Genetic engineering techniques have been applied in numerous fields including research, agriculture, industrial biotechnology, and medicine. Enzymes used in laundry detergent and medicines such as insulin and human growth hormone are now manufactured in GM cells, experimental GM cell lines and GM animals such as mice or zebrafish are being used for research purposes, and genetically modified crops have been commercialized.
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