High-Resolution Single-Copy Gene Fluorescence in Situ
... of pollen mother cells can be easily digested by enzyme treatment, thus facilitating probe penetration. Third, four copies of the DNA sequences of interest are present on pachytene bivalents, so signals will be stronger on pachytene chromosomes than on somatic metaphase chromosomes. Finally, the pac ...
... of pollen mother cells can be easily digested by enzyme treatment, thus facilitating probe penetration. Third, four copies of the DNA sequences of interest are present on pachytene bivalents, so signals will be stronger on pachytene chromosomes than on somatic metaphase chromosomes. Finally, the pac ...
Mutations in the MicroRNA Complementarity Site
... miRNA pathway. However, the demonstration that miR159 precursors are cleaved within the nucleus (Papp et al., 2003) suggested that HST might instead export mature miRNAs to the cytoplasm. Furthermore, although loss of function of HST causes a generalized reduction of miRNA levels, it does not cause ...
... miRNA pathway. However, the demonstration that miR159 precursors are cleaved within the nucleus (Papp et al., 2003) suggested that HST might instead export mature miRNAs to the cytoplasm. Furthermore, although loss of function of HST causes a generalized reduction of miRNA levels, it does not cause ...
Strong dominance of functional alleles over gene deletions in
... Table S1 shows that these factors were indeed often significant. We started by correcting for the time block and individual plate effect. We found that the upper quartile of a plate was less varying between plates that the mean or median. We therefore divided all individual MGR/MLSs by the upper qua ...
... Table S1 shows that these factors were indeed often significant. We started by correcting for the time block and individual plate effect. We found that the upper quartile of a plate was less varying between plates that the mean or median. We therefore divided all individual MGR/MLSs by the upper qua ...
Are you your grandmother`s favorite
... The MGM has two X-chromosomes, and so any given X-linked gene of hers has a 50 per cent chance of being transmitted to her daughter. Her daughter has one X from the MGM and one X from the maternal grandfather (‘MGF’). She will pass down one of those two X chromosomes to each child, regardless of whe ...
... The MGM has two X-chromosomes, and so any given X-linked gene of hers has a 50 per cent chance of being transmitted to her daughter. Her daughter has one X from the MGM and one X from the maternal grandfather (‘MGF’). She will pass down one of those two X chromosomes to each child, regardless of whe ...
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... substrate molecule H2AX is regulated at the transcription level and during chromatin packaging is completely unknown. If more H2AX molecules are newly synthesized, where should they be deposited on the genome for efficient protection from endogenous DSBs? While the genome-wide location of γ-H2AX has ...
... substrate molecule H2AX is regulated at the transcription level and during chromatin packaging is completely unknown. If more H2AX molecules are newly synthesized, where should they be deposited on the genome for efficient protection from endogenous DSBs? While the genome-wide location of γ-H2AX has ...
Document
... • Decreased P11 in the PFC of a genetic model of depression • ↓ mRNA and protein levels of P11 in the PFC of FSL compared to FRL • P11 mRNA between naive FSL & FRL not statistically significant with qRT-PCR – ↑ cellular resolution with in-situ hybridization ...
... • Decreased P11 in the PFC of a genetic model of depression • ↓ mRNA and protein levels of P11 in the PFC of FSL compared to FRL • P11 mRNA between naive FSL & FRL not statistically significant with qRT-PCR – ↑ cellular resolution with in-situ hybridization ...
Evolutionary Genomics of Fast Evolving Tunicates
... process of genome reduction could have been caused in part by the elimination of genes (like notochord genes and Hox genes, as described in the previous section), this was not the only or even the main cause, since this genome contains about 18,000 predicted genes. Instead, genome compaction, namely ...
... process of genome reduction could have been caused in part by the elimination of genes (like notochord genes and Hox genes, as described in the previous section), this was not the only or even the main cause, since this genome contains about 18,000 predicted genes. Instead, genome compaction, namely ...
16 Simple Patterns of Inheritance
... put forward in the 1860s by a researcher who knew nothing about chromosomes. Gregor Mendel, remembered today as the “father of genetics,” used statistical analysis of carefully designed plant breeding experiments to arrive at the concept of a gene, which is broadly defined as a unit of heredity. For ...
... put forward in the 1860s by a researcher who knew nothing about chromosomes. Gregor Mendel, remembered today as the “father of genetics,” used statistical analysis of carefully designed plant breeding experiments to arrive at the concept of a gene, which is broadly defined as a unit of heredity. For ...
The human Y chromosome: the biological role of a “functional
... and proximal deletion interval 6, and its molecular extension has been estimated to be similar to that of the AZFa region (1–3 Mb). Five genes have been so far described within this interval; RNA-binding motif (RBM), Chromodomain Y (CDY ), XK Related Y (XKRY ), eukaryotic translation initiation fact ...
... and proximal deletion interval 6, and its molecular extension has been estimated to be similar to that of the AZFa region (1–3 Mb). Five genes have been so far described within this interval; RNA-binding motif (RBM), Chromodomain Y (CDY ), XK Related Y (XKRY ), eukaryotic translation initiation fact ...
Hthsci 2231
... Her face is characteristic of her genetic abnormality. She had low nasal bridge, eyelids that are folded, a protruding tongue and flat, low set ears. She had already had surgery for a congenital heart defect. a. ...
... Her face is characteristic of her genetic abnormality. She had low nasal bridge, eyelids that are folded, a protruding tongue and flat, low set ears. She had already had surgery for a congenital heart defect. a. ...
GLYPHOSATE RESISTANCE Background / Problem
... Definition: chance changes in allele frequency that result from the sampling of gametes from generation to generation in a finite population Assume (for now) Hardy-Weinberg conditions Random mating ...
... Definition: chance changes in allele frequency that result from the sampling of gametes from generation to generation in a finite population Assume (for now) Hardy-Weinberg conditions Random mating ...
Genome Biology and Evolution
... Yucatán (Ben-Dov 2005). It is characterized by its use in the manufacture of native traditional crafts that provide an economic benefit to the local people. A yellow fat that is obtained from the female insect is used to prepare a lacquer to coat traditional art crafts making them resistant to heat ...
... Yucatán (Ben-Dov 2005). It is characterized by its use in the manufacture of native traditional crafts that provide an economic benefit to the local people. A yellow fat that is obtained from the female insect is used to prepare a lacquer to coat traditional art crafts making them resistant to heat ...
BIO 201 - Southern Union State Community College
... 6160 Field Biology. Intensive classroom and field studies of an area outside Alabama. BIOL BIOL 7090 Conservation Biology. This course is an overview of ethical, economic and biological aspects of conservation biology at scales ranging from local to global. BIOL 7300 Plant Anatomy and Development. ...
... 6160 Field Biology. Intensive classroom and field studies of an area outside Alabama. BIOL BIOL 7090 Conservation Biology. This course is an overview of ethical, economic and biological aspects of conservation biology at scales ranging from local to global. BIOL 7300 Plant Anatomy and Development. ...
Eyes Wide Open article
... chair of ophthalmology at Harvard. Hunter is a specialist in surgeries that, in some cases, can correct complex eye movement disorders. Patients come from around the world, and the two doctors pair up to consult with them—taking on only a couple of patients in an afternoon so they have ample time ...
... chair of ophthalmology at Harvard. Hunter is a specialist in surgeries that, in some cases, can correct complex eye movement disorders. Patients come from around the world, and the two doctors pair up to consult with them—taking on only a couple of patients in an afternoon so they have ample time ...
Overexpression of a Knotted-Like Homeobox Gene of Potato Alters
... An early-stage tuber cDNA library (Kang and Hannapel, 1995) from potato cv Superior was screened for members of the homeobox gene family. PCR primers were designed from the consensus sequence of the homeoboxes of the class I genes kn1 from maize (Vollbrecht et al., 1991), KNAT1 and KNAT2 from Arabid ...
... An early-stage tuber cDNA library (Kang and Hannapel, 1995) from potato cv Superior was screened for members of the homeobox gene family. PCR primers were designed from the consensus sequence of the homeoboxes of the class I genes kn1 from maize (Vollbrecht et al., 1991), KNAT1 and KNAT2 from Arabid ...
A Single Recessive Gene for Resistance to the Root
... Resistant F2 plants that were self-pollinated to make F3 families (F^ resistant families) produced progeny that were entirely resistant (Table 4), with gall indices ranging from 0 to 25%. Susceptible F2J families fit the expected ratio of 1 susceptible: 2 segregating (Table 4). The five crosses had ...
... Resistant F2 plants that were self-pollinated to make F3 families (F^ resistant families) produced progeny that were entirely resistant (Table 4), with gall indices ranging from 0 to 25%. Susceptible F2J families fit the expected ratio of 1 susceptible: 2 segregating (Table 4). The five crosses had ...
5 Genetic Analysis of Kidney Disease in Mice
... examples from several phenotypes. We begin by selecting QTL found in three or more crosses that also had a concordant human QTL. We first narrowed the region by comparative genomics if possible and then by statistically combining the crosses. Next, we used a dense SNP database to reduce the regions ...
... examples from several phenotypes. We begin by selecting QTL found in three or more crosses that also had a concordant human QTL. We first narrowed the region by comparative genomics if possible and then by statistically combining the crosses. Next, we used a dense SNP database to reduce the regions ...
Harnessing gene expression to identify the genetic basis of drug
... Box 1 (A) Growth in the presence of a subset of drugs is represented by the heat map on the left (blue corresponds to low growth yield and yellow to high growth yield). Each row represents the data for a single drug (SMP10 is 1,9-pyrazoloanthone, DFI is diphenyliodonium and SK&F is SK&F 96365) and e ...
... Box 1 (A) Growth in the presence of a subset of drugs is represented by the heat map on the left (blue corresponds to low growth yield and yellow to high growth yield). Each row represents the data for a single drug (SMP10 is 1,9-pyrazoloanthone, DFI is diphenyliodonium and SK&F is SK&F 96365) and e ...
Simulating evolution by gene duplication of protein features that
... would yield a null allele, are represented only implicitly in our computer model by the constant , which is the ratio of the number of mutations of the original duplicated gene that would produce a null allele to the number of mutations of the original duplicated gene that would yield a compatible ...
... would yield a null allele, are represented only implicitly in our computer model by the constant , which is the ratio of the number of mutations of the original duplicated gene that would produce a null allele to the number of mutations of the original duplicated gene that would yield a compatible ...
n - MrBrock.com
... Mendel’s Experimental, Quantitative Approach • Advantages of pea plants for genetic study: – There are many varieties with distinct heritable features, or characters (such as color); character variations are called traits – Mating of plants can be controlled – Each pea plant has sperm-producing org ...
... Mendel’s Experimental, Quantitative Approach • Advantages of pea plants for genetic study: – There are many varieties with distinct heritable features, or characters (such as color); character variations are called traits – Mating of plants can be controlled – Each pea plant has sperm-producing org ...
Characteristics of the gene encoding pyrroline-5-carboxylate synthase (P5CS) in Glycine max
... mechanisms which modified the structure of the protein P5CS (Boggess et al., 1976a, 1976b) [2], [3]. In Escherichia coli, the synthesis of proline is adjusted through the inhibition of activity of the enzyme γ-GK by the end product of the cycle that is proline, by which the activity of this enzyme i ...
... mechanisms which modified the structure of the protein P5CS (Boggess et al., 1976a, 1976b) [2], [3]. In Escherichia coli, the synthesis of proline is adjusted through the inhibition of activity of the enzyme γ-GK by the end product of the cycle that is proline, by which the activity of this enzyme i ...
Goldmine: Integrating information to place sets of genomic ranges
... proportions across the range set is also demonstrated. A list of variable-size genomic ranges representing regions with changes in DNA methylation is used as an example. This query range set can be interchanged for any set of interest. ...
... proportions across the range set is also demonstrated. A list of variable-size genomic ranges representing regions with changes in DNA methylation is used as an example. This query range set can be interchanged for any set of interest. ...
Article The Landscape of Realized Homologous
... (Perez-Losada et al. 2006; Vos 2009), ranging from clonal species (Smith et al. 2006) to those that exchange 10% or more of their DNA within a single 4-year human infection (Cao et al. 2014). This variation presents specific challenges for effective comparison between species. We recently developed ...
... (Perez-Losada et al. 2006; Vos 2009), ranging from clonal species (Smith et al. 2006) to those that exchange 10% or more of their DNA within a single 4-year human infection (Cao et al. 2014). This variation presents specific challenges for effective comparison between species. We recently developed ...
Marker-assisted backcross breeding
... Background selection in BC2F2: ~350 plants, genotyping with ~300 genomewide SNPs plus QTL markers and select 5 BC2F2 plants with fixed 3 QTLs and 90-92% recurrent genome in BC2F2 increased to >95% recurrent genome in BC2F3 ...
... Background selection in BC2F2: ~350 plants, genotyping with ~300 genomewide SNPs plus QTL markers and select 5 BC2F2 plants with fixed 3 QTLs and 90-92% recurrent genome in BC2F2 increased to >95% recurrent genome in BC2F3 ...
Chromosomal Theory and Genetic Linkage
... Figure 2: Inheritance patterns of unlinked and linked genes are shown. In (a), two genes are located on dierent chromosomes so independent assortment occurs during meiosis. The ospring have an equal chance of being the parental type (inheriting the same combination of traits as the parents) or a n ...
... Figure 2: Inheritance patterns of unlinked and linked genes are shown. In (a), two genes are located on dierent chromosomes so independent assortment occurs during meiosis. The ospring have an equal chance of being the parental type (inheriting the same combination of traits as the parents) or a n ...
Genetic engineering
Genetic engineering, also called genetic modification, is the direct manipulation of an organism's genome using biotechnology. It is therefore a set of technologies used to change the genetic makeup of cells, including the transfer of genes within and across species boundaries to produce improved or novel organisms. New DNA may be inserted in the host genome by first isolating and copying the genetic material of interest using molecular cloning methods to generate a DNA sequence, or by synthesizing the DNA, and then inserting this construct into the host organism. Genes may be removed, or ""knocked out"", using a nuclease. Gene targeting is a different technique that uses homologous recombination to change an endogenous gene, and can be used to delete a gene, remove exons, add a gene, or introduce point mutations.An organism that is generated through genetic engineering is considered to be a genetically modified organism (GMO). The first GMOs were bacteria generated in 1973 and GM mice in 1974. Insulin-producing bacteria were commercialized in 1982 and genetically modified food has been sold since 1994. Glofish, the first GMO designed as a pet, was first sold in the United States December in 2003.Genetic engineering techniques have been applied in numerous fields including research, agriculture, industrial biotechnology, and medicine. Enzymes used in laundry detergent and medicines such as insulin and human growth hormone are now manufactured in GM cells, experimental GM cell lines and GM animals such as mice or zebrafish are being used for research purposes, and genetically modified crops have been commercialized.