
Measurement of the 4Pi-confocal point spread function proves 75
... scanned in a plane containing the optical axis and the scattered light intensity was recorded as a function of the position. Scanning was performed by a scan stage that offered independently controlled x, y, and z axes (Photon Control, Cambridge, England). The bead was scanned 1.5 pm along the optic ...
... scanned in a plane containing the optical axis and the scattered light intensity was recorded as a function of the position. Scanning was performed by a scan stage that offered independently controlled x, y, and z axes (Photon Control, Cambridge, England). The bead was scanned 1.5 pm along the optic ...
Optics in Confocal Microscopy
... The relationship between NA and resolution For lenses in air NA = 1 / 2F, where F = the F number of the lens. (i.e. an objective of NA 0.5 has an F number of one). Ernst Abbe introduced this term because he realised that it is proportional to the (lateral) resolving power of the lens, according to ...
... The relationship between NA and resolution For lenses in air NA = 1 / 2F, where F = the F number of the lens. (i.e. an objective of NA 0.5 has an F number of one). Ernst Abbe introduced this term because he realised that it is proportional to the (lateral) resolving power of the lens, according to ...
PHYSICS 504 OPTICS REVIEW: Important things to remember: 1
... calculations, explain how it is possible for Marlin and Nemo to see each other from their present positions. Justify your answer. Air ...
... calculations, explain how it is possible for Marlin and Nemo to see each other from their present positions. Justify your answer. Air ...
pupil function - UCT Digital Image Processing
... As long as the CCD is exposed to light, charge accumulates in the potential wells. Apart from any effects due to saturation, the charge is proportional to the number of light photons incident on the specific location on the CCD. In the simplest case (the full-frame CCD), readout is performed by shut ...
... As long as the CCD is exposed to light, charge accumulates in the potential wells. Apart from any effects due to saturation, the charge is proportional to the number of light photons incident on the specific location on the CCD. In the simplest case (the full-frame CCD), readout is performed by shut ...
Aperture

In optics, an aperture is a hole or an opening through which light travels. More specifically, the aperture and focal length of an optical system determine the cone angle of a bundle of rays that come to a focus in the image plane. The aperture determines how collimated the admitted rays are, which is of great importance for the appearance at the image plane. If an aperture is narrow, then highly collimated rays are admitted, resulting in a sharp focus at the image plane. If an aperture is wide, then uncollimated rays are admitted, resulting in a sharp focus only for rays with a certain focal length. This means that a wide aperture results in an image that is sharp for things at the correct distance. The aperture also determines how many of the incoming rays are actually admitted and thus how much light reaches the image plane (the narrower the aperture, the darker the image for a given exposure time). In the human eye, the pupil is the aperture.An optical system typically has many openings, or structures that limit the ray bundles (ray bundles are also known as pencils of light). These structures may be the edge of a lens or mirror, or a ring or other fixture that holds an optical element in place, or may be a special element such as a diaphragm placed in the optical path to limit the light admitted by the system. In general, these structures are called stops, and the aperture stop is the stop that determines the ray cone angle, or equivalently the brightness, at an image point.In some contexts, especially in photography and astronomy, aperture refers to the diameter of the aperture stop rather than the physical stop or the opening itself. For example, in a telescope the aperture stop is typically the edges of the objective lens or mirror (or of the mount that holds it). One then speaks of a telescope as having, for example, a 100 centimeter aperture. Note that the aperture stop is not necessarily the smallest stop in the system. Magnification and demagnification by lenses and other elements can cause a relatively large stop to be the aperture stop for the system.Sometimes stops and diaphragms are called apertures, even when they are not the aperture stop of the system.The word aperture is also used in other contexts to indicate a system which blocks off light outside a certain region. In astronomy for example, a photometric aperture around a star usually corresponds to a circular window around the image of a star within which the light intensity is assumed.