Extending the Effective Ranging Depth of Spectral Domain Optical
... reference paths with different offset delays [2]. Recently, Ruggeriet al. enabled OCT imaging of multi-frames at four depths without sacrificing axial resolution by implementing a galvo scanner-based mechanical optical switch in the reference arm [15]. They utilized the system to image the whole eye ...
... reference paths with different offset delays [2]. Recently, Ruggeriet al. enabled OCT imaging of multi-frames at four depths without sacrificing axial resolution by implementing a galvo scanner-based mechanical optical switch in the reference arm [15]. They utilized the system to image the whole eye ...
Soft x-ray laser holography with wavelength resolution *
... temporal and spatial coherence of the illuminating source, the resolution of the medium in which the hologram is recorded, and the digitization process used are also factors that influence the ultimate resolution. The experimental setup described in this paper was optimized to limit the degrading ef ...
... temporal and spatial coherence of the illuminating source, the resolution of the medium in which the hologram is recorded, and the digitization process used are also factors that influence the ultimate resolution. The experimental setup described in this paper was optimized to limit the degrading ef ...
High resolution spectral self-interference fluorescence microscopy
... The advantage of light microscopy over electron and scanned probe microscopy is the ability to image the interior of biological specimens. Light would be an ideal carrier of microscopic information if it weren’t for the resolution limit. But because of diffraction, standard light microscopes are not ...
... The advantage of light microscopy over electron and scanned probe microscopy is the ability to image the interior of biological specimens. Light would be an ideal carrier of microscopic information if it weren’t for the resolution limit. But because of diffraction, standard light microscopes are not ...
High-speed addressable confocal microscopy for functional imaging
... particular pixel, is by simultaneously scanning several sufficiently spaced spots, as is done with Nipkow disk-based scanners.8,9 By scanning ⬃20,000 fixed-size pinholes, systems based on such scanners have reported frame rates of ⬃1 kHz.10–12 However, a limitation in placing multiple excitation spo ...
... particular pixel, is by simultaneously scanning several sufficiently spaced spots, as is done with Nipkow disk-based scanners.8,9 By scanning ⬃20,000 fixed-size pinholes, systems based on such scanners have reported frame rates of ⬃1 kHz.10–12 However, a limitation in placing multiple excitation spo ...
Super-resolution Microscopy
... spaced point sources are imaged at different times. That is, imagine two PSFs that cannot be otherwise distinguished but each is observed at a different time (Fig. 3D). This methodology of “sequential” imaging at different times forms the basis of the most recent and successful superresolution micro ...
... spaced point sources are imaged at different times. That is, imagine two PSFs that cannot be otherwise distinguished but each is observed at a different time (Fig. 3D). This methodology of “sequential” imaging at different times forms the basis of the most recent and successful superresolution micro ...
pdf.file
... the water, and especially through the air-water interface. Our simulation methodology is part of a more general approach to underwater imaging, which has been of particular interest to researchers in fields such as marine science and military defense and we believe that our results will be useful in ...
... the water, and especially through the air-water interface. Our simulation methodology is part of a more general approach to underwater imaging, which has been of particular interest to researchers in fields such as marine science and military defense and we believe that our results will be useful in ...
Get PDF - OSA Publishing
... solutions lead to more expensive and harder to build spectrometers. Our design proposal is applied to the standard Offner spectrometer. So we can obtain simpler, easier to align and cheaper spectrometers. It is a further advantage in addition to the above mentioned ones. The study of an optical syst ...
... solutions lead to more expensive and harder to build spectrometers. Our design proposal is applied to the standard Offner spectrometer. So we can obtain simpler, easier to align and cheaper spectrometers. It is a further advantage in addition to the above mentioned ones. The study of an optical syst ...
remote sensing
... sensing to date have been based on observing crops in distinct areas of the electromagnetic spectrum. The spectrum is represented in the figure below. Agricultural remote sensing is commonly done in the visible, near-infrared and thermal infrared portions of the spectrum; however, new applications i ...
... sensing to date have been based on observing crops in distinct areas of the electromagnetic spectrum. The spectrum is represented in the figure below. Agricultural remote sensing is commonly done in the visible, near-infrared and thermal infrared portions of the spectrum; however, new applications i ...
Adiabatic far-field sub-diffraction imaging ARTICLE Hu Cang *, Alessandro Salandrino
... lthough a tsunami is devastating near land, it is barely noticeable in the open ocean. This is because the sea floor near the land slows down the tsunami, compressing its wavelength from hundreds of kilometres1 to metres and rapidly increasing its amplitude to be destructive. Recently, a similar opti ...
... lthough a tsunami is devastating near land, it is barely noticeable in the open ocean. This is because the sea floor near the land slows down the tsunami, compressing its wavelength from hundreds of kilometres1 to metres and rapidly increasing its amplitude to be destructive. Recently, a similar opti ...
Light Microscopy [10 credits]
... Explain the role of numerical aperture in imaging Demonstrate the condenser’s role in image formation Lecture 3 Title: Optical contrast techniques Description: Dark-field microscopy; phase contrast microscopy; differential interference contrast microscopy Author(s): Prof Andrew Jarman Learning O ...
... Explain the role of numerical aperture in imaging Demonstrate the condenser’s role in image formation Lecture 3 Title: Optical contrast techniques Description: Dark-field microscopy; phase contrast microscopy; differential interference contrast microscopy Author(s): Prof Andrew Jarman Learning O ...
Edge-enhanced imaging with polyvinyl alcohol/acrylamide photopolymer gratings 1510
... frequency of 1125 lines兾mm. In Fig. 2 we show the angular response (in intensity) of the transmitted order as a function of reconstruction angle ur for a He –Ne laser beam. We see that the Bragg angle is 20.8± with a diffraction efficiency of 93%, close to the theoretical maximum of 100%. Circles re ...
... frequency of 1125 lines兾mm. In Fig. 2 we show the angular response (in intensity) of the transmitted order as a function of reconstruction angle ur for a He –Ne laser beam. We see that the Bragg angle is 20.8± with a diffraction efficiency of 93%, close to the theoretical maximum of 100%. Circles re ...
Noniterative Exact Solution to the Phase Problem in Optical Imaging Implemented with Scanning Probe Microscope.
... As is well-known, the basis for obtaining superresolution is multiple images.20 This of course can be obtained in a cumbersome way by moving the sample, which would require the accurate verification of the exact sample movement. This could be done in our method by the presence of an online AFM. Howev ...
... As is well-known, the basis for obtaining superresolution is multiple images.20 This of course can be obtained in a cumbersome way by moving the sample, which would require the accurate verification of the exact sample movement. This could be done in our method by the presence of an online AFM. Howev ...
X-ray phase contrast microscopy at 300 nm
... see Scanning Electron Micrographs of the three structures in Fig. 3A–3C. We used in this case a WG with 300 nm gap size. The measurements are reported in Fig. 3D–3F. All images were acquired with z1 = 2 mm and z1 + z2 = 450 mm, therefore with a constant magnification M = 225. The exposure time was 6 ...
... see Scanning Electron Micrographs of the three structures in Fig. 3A–3C. We used in this case a WG with 300 nm gap size. The measurements are reported in Fig. 3D–3F. All images were acquired with z1 = 2 mm and z1 + z2 = 450 mm, therefore with a constant magnification M = 225. The exposure time was 6 ...
Partially Coherent Image Formation Theory for X
... enabled development of short wavelenth focusing elements and significantly improved the spatial resolution [4–9]. In the soft x-ray spectral region, samples as small as a few tens of nanometers can be resolved using micro zone-plates (MZP) as the objective lens [3]. In addition to conventional x-ray ...
... enabled development of short wavelenth focusing elements and significantly improved the spatial resolution [4–9]. In the soft x-ray spectral region, samples as small as a few tens of nanometers can be resolved using micro zone-plates (MZP) as the objective lens [3]. In addition to conventional x-ray ...
Biology 177: Principles of Modern Microscopy
... NSOM disadvantages • Practically zero working distance and small depth of field. • Extremely long scan times for high resolution images or large specimen areas. ...
... NSOM disadvantages • Practically zero working distance and small depth of field. • Extremely long scan times for high resolution images or large specimen areas. ...
as a PDF - Department of Engineering Science
... can also suffer from other disadvantages, which make imaging of delicate samples, such as live cell cultures, very difficult. An alternative focusing method that does not involve mechanical movements near the specimen is clearly preferable. This would avoid specimen agitation and potentially permit ...
... can also suffer from other disadvantages, which make imaging of delicate samples, such as live cell cultures, very difficult. An alternative focusing method that does not involve mechanical movements near the specimen is clearly preferable. This would avoid specimen agitation and potentially permit ...
X- and Gamma Ray Imaging Systems based on CdTe-CMOS
... circuitry and real time calibration result in exquisite performance (high DQE) throughout a wide X-ray energy window (10 – 300 keV) and superb image quality very close to the theoretical ideal. Additionally, these sensor systems exhibit good stability over time and negligible afterglow. System level ...
... circuitry and real time calibration result in exquisite performance (high DQE) throughout a wide X-ray energy window (10 – 300 keV) and superb image quality very close to the theoretical ideal. Additionally, these sensor systems exhibit good stability over time and negligible afterglow. System level ...
3D differential interference contrast microscopy using synthetic
... Differential interference contrast microscopy techniques visualize a specimen of weak contrast by converting the field gradient of a light into intensity variation. It has served as a label-free tool to image biological specimens.1,2 However, the intensity in the DIC image is determined by the coupl ...
... Differential interference contrast microscopy techniques visualize a specimen of weak contrast by converting the field gradient of a light into intensity variation. It has served as a label-free tool to image biological specimens.1,2 However, the intensity in the DIC image is determined by the coupl ...
Principles, Instrumentation, and Applications of Infrared Multispectral
... such, a single data set consists of both spatial and spectral information. The typical dimensions of this data set range from 256 256 to 1024 1024 pixels, with each pixel containing a full IR spectrum. Such data will facilitate visualizing chemical distribution in a sample or chemical compositio ...
... such, a single data set consists of both spatial and spectral information. The typical dimensions of this data set range from 256 256 to 1024 1024 pixels, with each pixel containing a full IR spectrum. Such data will facilitate visualizing chemical distribution in a sample or chemical compositio ...
White-light diffraction tomography of unlabelled live cells
... white-light diffraction tomography (WDT). WDT offers a high-performance, simple design, as well as suitability for operation in a conventional microscopy setting. Its main features can be summarized as follows. First, WDT is a generalization of diffraction tomography to broadband illumination. Secon ...
... white-light diffraction tomography (WDT). WDT offers a high-performance, simple design, as well as suitability for operation in a conventional microscopy setting. Its main features can be summarized as follows. First, WDT is a generalization of diffraction tomography to broadband illumination. Secon ...
Aberration-free three-dimensional multiphoton imaging of neuronal
... rates along the z direction compared to the kHz rates obtainable in the x and y directions. Here, we describe a custom-built microscope system based on an architecture that allows kHz scan rates over hundreds of microns in all three dimensions without introducing aberration. We further demonstrate h ...
... rates along the z direction compared to the kHz rates obtainable in the x and y directions. Here, we describe a custom-built microscope system based on an architecture that allows kHz scan rates over hundreds of microns in all three dimensions without introducing aberration. We further demonstrate h ...
Νευροφυσιολογία και Αισθήσεις Response to Current Injection
... predict a stable resting membrane potential of -75 mV, as is seen in many cells ...
... predict a stable resting membrane potential of -75 mV, as is seen in many cells ...
Infrared camera ME207
... Infrared energy is just one part of the electromagnetic spectrum that encompasses radiation from gamma rays, x-rays, ultra violet, a thin region of visible light, infrared, terahertz waves, microwaves, and radio waves. These are all related and differentiated in the length of their wave (wavelength) ...
... Infrared energy is just one part of the electromagnetic spectrum that encompasses radiation from gamma rays, x-rays, ultra violet, a thin region of visible light, infrared, terahertz waves, microwaves, and radio waves. These are all related and differentiated in the length of their wave (wavelength) ...
Telescopes for CCD Imaging
... sometimes shifts when you point to different positions in the sky. SCT’s are difficult to focus. It is difficult to tell when you are in focus. A separate electronic focuser for close focusing, that does not move the primary, such as the JMI NGF-S or Optec TCF is highly desirable in this context. So ...
... sometimes shifts when you point to different positions in the sky. SCT’s are difficult to focus. It is difficult to tell when you are in focus. A separate electronic focuser for close focusing, that does not move the primary, such as the JMI NGF-S or Optec TCF is highly desirable in this context. So ...
Hyperspectral imaging
Hyperspectral imaging, like other spectral imaging, collects and processes information from across the electromagnetic spectrum. The goal of hyperspectral imaging is to obtain the spectrum for each pixel in the image of a scene, with the purpose of finding objects, identifying materials, or detecting processes.Much as the human eye sees visible light in three bands (red, green, and blue), spectral imaging divides the spectrum into many more bands. This technique of dividing images into bands can be extended beyond the visible. In hyperspectral imaging, the recorded spectra have fine wavelength resolution and cover a wide range of wavelengths.Engineers build hyperspectral sensors and processing systems for applications in astronomy, agriculture, biomedical imaging, geosciences, physics, and surveillance. Hyperspectral sensors look at objects using a vast portion of the electromagnetic spectrum. Certain objects leave unique 'fingerprints' in the electromagnetic spectrum. Known as spectral signatures, these 'fingerprints' enable identification of the materials that make up a scanned object. For example, a spectral signature for oil helps geologists find new oil fields.