Growth and killing of a Salmonella enterica serovar
... (wild-type strain) or 10' c.f.u. per mouse (ssaV or sifA mutant strains), as described previously (Beuzo! n et al., 2000). Spleens were removed aseptically 3 days after inoculation, and placed in 2 ml ice-cold PBS. Cell suspensions were obtained as described previously (Salcedo et al., 2001). Briefl ...
... (wild-type strain) or 10' c.f.u. per mouse (ssaV or sifA mutant strains), as described previously (Beuzo! n et al., 2000). Spleens were removed aseptically 3 days after inoculation, and placed in 2 ml ice-cold PBS. Cell suspensions were obtained as described previously (Salcedo et al., 2001). Briefl ...
SAM Teachers Guide Lipids and Carbohydrates - RI
... This activity focuses on the basic structure and function of both lipids and carbohydrates, two of the four major macromolecule families in biological systems. Students will look specifically at the polarity of the molecules and how polarity affects their solubility and behavior in different envi ...
... This activity focuses on the basic structure and function of both lipids and carbohydrates, two of the four major macromolecule families in biological systems. Students will look specifically at the polarity of the molecules and how polarity affects their solubility and behavior in different envi ...
Chapter Assessment
... Diffusion limits cell size • Although diffusion is a fast and efficient process over short distances, it becomes slow and inefficient as the distances become larger. • Because of the slow rate of diffusion, organisms can’t be just one giant-sized cell. ...
... Diffusion limits cell size • Although diffusion is a fast and efficient process over short distances, it becomes slow and inefficient as the distances become larger. • Because of the slow rate of diffusion, organisms can’t be just one giant-sized cell. ...
+ -80 mV
... •Derives from the Nernst-Planck equation and a few assumptions •Uses permeabilities rather than conductances •Cl- is flipped to account for a -1 valence ...
... •Derives from the Nernst-Planck equation and a few assumptions •Uses permeabilities rather than conductances •Cl- is flipped to account for a -1 valence ...
CHAPTER 3 LEARNING OBJECTIVES -
... General remarks Be able to define protoplast Know components found in every bacterial cell Plasma (cell or cytoplasmic) membrane, ribosomes, chromosome (nucleoid), protoplasm, enzymes Most also have a cell wall and periplasm Know bacteria are prokaryotic, and thus lack membrane-bound org ...
... General remarks Be able to define protoplast Know components found in every bacterial cell Plasma (cell or cytoplasmic) membrane, ribosomes, chromosome (nucleoid), protoplasm, enzymes Most also have a cell wall and periplasm Know bacteria are prokaryotic, and thus lack membrane-bound org ...
Functional enhancement of neuronal cell behaviors and
... elastin-mimetic recombinant protein, TGPG[VGRGD(VGVPG)6]20WPC, referred to as [RGD-V6]20, contains multiple RGD motifs to bind cell-surface integrins. This study aimed to investigate how surface-adsorbed recombinant protein can be used to modulate the behaviors and differentiation of neuronal cells ...
... elastin-mimetic recombinant protein, TGPG[VGRGD(VGVPG)6]20WPC, referred to as [RGD-V6]20, contains multiple RGD motifs to bind cell-surface integrins. This study aimed to investigate how surface-adsorbed recombinant protein can be used to modulate the behaviors and differentiation of neuronal cells ...
Chapter 4 – A Tour of the Cell
... existed as free-living prokaryotes which were engulfed and retained by ancient eukaryotic cells approximately 1.5 billion years ago. • Chloroplasts, the organelles ...
... existed as free-living prokaryotes which were engulfed and retained by ancient eukaryotic cells approximately 1.5 billion years ago. • Chloroplasts, the organelles ...
Correct Proteolytic Cleavage Is Required for the Cell Adhesive
... several potential trypsin cleavage sites are located. One of these sites either in the precursor region or in the mature protein part could be used by trypsin to generate a 120-kD protein from the LF-7 mutant polypeptide. One striking characteristic of all Ca2+-dependent CAMs is that the extracellul ...
... several potential trypsin cleavage sites are located. One of these sites either in the precursor region or in the mature protein part could be used by trypsin to generate a 120-kD protein from the LF-7 mutant polypeptide. One striking characteristic of all Ca2+-dependent CAMs is that the extracellul ...
Synaptic transmission disorder
... Vesicles are guided toward membrane by proteins Guilding proteins act like ropes that help to pull the vesicle and presynaptic membrane together An influx of calcium ions into the presynaptic terminal but ...
... Vesicles are guided toward membrane by proteins Guilding proteins act like ropes that help to pull the vesicle and presynaptic membrane together An influx of calcium ions into the presynaptic terminal but ...
Logic of Definitions
... • definitions are in principle ineliminable; we can unpack the texts which contain them completely, without changing the truth value at all (definitions are in this sense dispensable abbreviations) ...
... • definitions are in principle ineliminable; we can unpack the texts which contain them completely, without changing the truth value at all (definitions are in this sense dispensable abbreviations) ...
Lipid Map of the Mammalian Cell
... then their local organization within each membrane. To understand lipid homeostasis and its dynamics, one has to study the localized metabolism of lipids, their transport within and between the various membranes, and the sensors and effectors that govern these processes. In terms of function, above ...
... then their local organization within each membrane. To understand lipid homeostasis and its dynamics, one has to study the localized metabolism of lipids, their transport within and between the various membranes, and the sensors and effectors that govern these processes. In terms of function, above ...
Cell Processes Review
... ____ 14. Many substances move through cell membranes from areas of higher concentration to areas of lower concentration in the process of a. DNA replication. b. diffusion. c. reproduction. d. cellular respiration. ____ 15. What is the function of a cell membrane? a. to perform different functions in ...
... ____ 14. Many substances move through cell membranes from areas of higher concentration to areas of lower concentration in the process of a. DNA replication. b. diffusion. c. reproduction. d. cellular respiration. ____ 15. What is the function of a cell membrane? a. to perform different functions in ...
Journal of Applied Biomedicine REVIEW Cell wall
... (Holubářová 2002). However, the same antibodies failed to bind to any surface structures in yeast cells, as seen in the fluorescence microscope. The genome databases Blast, Fasta and Proteome did not revealed any S. cerevisiae sequence homology with human or chicken integrins (Holubářová 2002). In A ...
... (Holubářová 2002). However, the same antibodies failed to bind to any surface structures in yeast cells, as seen in the fluorescence microscope. The genome databases Blast, Fasta and Proteome did not revealed any S. cerevisiae sequence homology with human or chicken integrins (Holubářová 2002). In A ...
Cell wall-cytoplasm signalling
... bud growth, is modified by the addition of bud scars, and becomes more refractory as it ages (Lipke and Ovalle 1998, de Nobel et al. 2000). The wall is remodelled during mating, cell fusion, pseudohyphal formation and spore germination. This remodelling is analogous to wall softening in plant cells ...
... bud growth, is modified by the addition of bud scars, and becomes more refractory as it ages (Lipke and Ovalle 1998, de Nobel et al. 2000). The wall is remodelled during mating, cell fusion, pseudohyphal formation and spore germination. This remodelling is analogous to wall softening in plant cells ...
AP lab 1 Osmosis
... to an area of low concentration. This phenomenon is known as diffusion. In living things, cell membranes prevent most molecules from simply diffusing down their concentration gradient. This allows the living thing (the smallest of which is a cell) to maintain an internal environment that is differen ...
... to an area of low concentration. This phenomenon is known as diffusion. In living things, cell membranes prevent most molecules from simply diffusing down their concentration gradient. This allows the living thing (the smallest of which is a cell) to maintain an internal environment that is differen ...
Programmed Cell Death in Development and
... first and primarily from work on leaf senescence. Yoshio Yoshida elegantly showed that the nucleus is required for cellular disassembly (21) and so it is not surprising that many labs independently showed that inhibitors of protein translation block leaf senescence. Non-senescencing (stay-green) mut ...
... first and primarily from work on leaf senescence. Yoshio Yoshida elegantly showed that the nucleus is required for cellular disassembly (21) and so it is not surprising that many labs independently showed that inhibitors of protein translation block leaf senescence. Non-senescencing (stay-green) mut ...
Protein import into the nucleus - Universitätsklinikum des Saarlandes
... Fig. 2. Schematic model of protein import into the nucleus. The ct-subunit of the NLS-receptor (NRet) associates with the nuclear localization sequence (NLS) of an import substrate. NRet can bind to pore-associated or cytoplasmic NRI3. The NR heterodimer binds via NRI3 to FG repeats of a subset of n ...
... Fig. 2. Schematic model of protein import into the nucleus. The ct-subunit of the NLS-receptor (NRet) associates with the nuclear localization sequence (NLS) of an import substrate. NRet can bind to pore-associated or cytoplasmic NRI3. The NR heterodimer binds via NRI3 to FG repeats of a subset of n ...
Lab 14 Review Name: Osmosis Instructions: Log in to www
... 1. Choose one a double layered membrane that surrounds the cell and also called the plasma membrane. Regulates what enters and exits the cell. 2. Choose one a measure of how much a given substance is mixed with another substance. 3. Choose one The spontaneous net movement of particles from area of h ...
... 1. Choose one a double layered membrane that surrounds the cell and also called the plasma membrane. Regulates what enters and exits the cell. 2. Choose one a measure of how much a given substance is mixed with another substance. 3. Choose one The spontaneous net movement of particles from area of h ...
6-9_IonChannelsPatchClamp_TasiBenedekJozsef
... The „patch clamp” is a technique in electrophysiology that allows the study of single or multiple ion channels in a wide variety of cells. It is technically a refinement of the Voltage clamp, an experimental method which measures the ion currents through cell membranes. The patch clamp was developed ...
... The „patch clamp” is a technique in electrophysiology that allows the study of single or multiple ion channels in a wide variety of cells. It is technically a refinement of the Voltage clamp, an experimental method which measures the ion currents through cell membranes. The patch clamp was developed ...
A Physicist Looks at Biology
... bacterium, the infecting particles must be greatly modified if not actually broken down into subunits. It seems that the real reproduction and recombination has already occurred during the first dark period when no active particle can be detected, because when the first complete particles appear the ...
... bacterium, the infecting particles must be greatly modified if not actually broken down into subunits. It seems that the real reproduction and recombination has already occurred during the first dark period when no active particle can be detected, because when the first complete particles appear the ...
Chapter 36 Part I Transport in Vascular Plants
... pumps. This promotes the uptake of K+, lowering the water potential. Water moves from high to low potential causing the guard cells to swell and ...
... pumps. This promotes the uptake of K+, lowering the water potential. Water moves from high to low potential causing the guard cells to swell and ...
Molecular Biology of the Cell
... plasma membrane (Fig 10-3, Fig 10-16) Marker for apoptosis Signal to neighboring cells, macrophages (“Eat me” signal) ...
... plasma membrane (Fig 10-3, Fig 10-16) Marker for apoptosis Signal to neighboring cells, macrophages (“Eat me” signal) ...
Cytosol
The cytosol or intracellular fluid (ICF) or cytoplasmic matrix is the liquid found inside cells. It is separated into compartments by membranes. For example, the mitochondrial matrix separates the mitochondrion into many compartments.In the eukaryotic cell, the cytosol is within the cell membrane and is part of the cytoplasm, which also comprises the mitochondria, plastids, and other organelles (but not their internal fluids and structures); the cell nucleus is separate. In prokaryotes, most of the chemical reactions of metabolism take place in the cytosol, while a few take place in membranes or in the periplasmic space. In eukaryotes, while many metabolic pathways still occur in the cytosol, others are contained within organelles.The cytosol is a complex mixture of substances dissolved in water. Although water forms the large majority of the cytosol, its structure and properties within cells is not well understood. The concentrations of ions such as sodium and potassium are different in the cytosol than in the extracellular fluid; these differences in ion levels are important in processes such as osmoregulation, cell signaling, and the generation of action potentials in excitable cells such as endocrine, nerve and muscle cells. The cytosol also contains large amounts of macromolecules, which can alter how molecules behave, through macromolecular crowding.Although it was once thought to be a simple solution of molecules, the cytosol has multiple levels of organization. These include concentration gradients of small molecules such as calcium, large complexes of enzymes that act together to carry out metabolic pathways, and protein complexes such as proteasomes and carboxysomes that enclose and separate parts of the cytosol.