lecture 17
... involves proteasome (in archaea) or other ATP-dependent proteases in archaea/bacteria proteasomes/other oligomeric proteases digest proteins to small peptides tricorn protease then cleaves these to 2-4 mers, which are then degraded down to the level of free amino acids by aminopeptidases ...
... involves proteasome (in archaea) or other ATP-dependent proteases in archaea/bacteria proteasomes/other oligomeric proteases digest proteins to small peptides tricorn protease then cleaves these to 2-4 mers, which are then degraded down to the level of free amino acids by aminopeptidases ...
CD63 Is a Component of Weibel
... tubes (Nalge, Rochester, NY). A gradient was generated by centrifugation at 40,000gm, in a Sorvall RC-SB centrifuge (SM24 rotor). To recover purified Weibel-Palade bodies free of Percoll, the lower third of the two gradientswas transferred to a fresh tube and mixed with Nycodenz (Sigma; from an 80% ...
... tubes (Nalge, Rochester, NY). A gradient was generated by centrifugation at 40,000gm, in a Sorvall RC-SB centrifuge (SM24 rotor). To recover purified Weibel-Palade bodies free of Percoll, the lower third of the two gradientswas transferred to a fresh tube and mixed with Nycodenz (Sigma; from an 80% ...
Cellular Membranes
... – area of greater amount vs. area of lesser amount – the difference may be LARGE or small – may exist across a physical barrier (membrane) or across a distance without a barrier • Substances in an area of high concentration naturally move toward regions of lower concentrations – Form of mechanical e ...
... – area of greater amount vs. area of lesser amount – the difference may be LARGE or small – may exist across a physical barrier (membrane) or across a distance without a barrier • Substances in an area of high concentration naturally move toward regions of lower concentrations – Form of mechanical e ...
Honors Biology Topic #3: Eukaryotic Kingdoms
... 4) Which of the eukaryotic kingdoms contain BOTH unicellular and multicellular organisms? Protista and Fungi 5) Which of the eukaryotic kingdoms contain organisms that are autotrophs? some protists and all plants (NO fungi or animals) 6) What does “autotroph” mean? an organism that can make its own ...
... 4) Which of the eukaryotic kingdoms contain BOTH unicellular and multicellular organisms? Protista and Fungi 5) Which of the eukaryotic kingdoms contain organisms that are autotrophs? some protists and all plants (NO fungi or animals) 6) What does “autotroph” mean? an organism that can make its own ...
Science Cell Parts Project
... Create either an animal or plant cell map. Use the organelle list provided as a list of locations within the cell that would be found on a map. Create a legend to help other’s know how to read the map. For example, cytoplasm is streaming or moving like a river inside of the cell. Draw the cytoplasm ...
... Create either an animal or plant cell map. Use the organelle list provided as a list of locations within the cell that would be found on a map. Create a legend to help other’s know how to read the map. For example, cytoplasm is streaming or moving like a river inside of the cell. Draw the cytoplasm ...
AP Biology Unit 2 Exam Study Guide 1. Explain the requirements for
... 23. Use the Laws of Thermodynamics to explain the flow of energy through a food web. 24. Explain the role of the substances which make up the structure of plant cells. 25. Explain how and why the location of ribosomes is important in protein synthesis. 26. Explain which techniques would be best for ...
... 23. Use the Laws of Thermodynamics to explain the flow of energy through a food web. 24. Explain the role of the substances which make up the structure of plant cells. 25. Explain how and why the location of ribosomes is important in protein synthesis. 26. Explain which techniques would be best for ...
Living Things
... All cells contain carbohydrates, lipids, proteins, and nucleic acids, as well as water and other inorganic compounds. But do all cells contain the same percentages of these compounds? The graph compares the percentage of some compounds found in a bacterial cell and a cell from a ...
... All cells contain carbohydrates, lipids, proteins, and nucleic acids, as well as water and other inorganic compounds. But do all cells contain the same percentages of these compounds? The graph compares the percentage of some compounds found in a bacterial cell and a cell from a ...
template for Microsoft Word
... but, when a substrate more highly oxidized than glucose, such as pyruvate, is the precursor, there is no direct cytoplasmic source of NADH2. Quantitative data on the fate of pyruvate are in accord with the conclusion that the NADH2 was primarily formed intramitochondrially by the dehydrogenases of c ...
... but, when a substrate more highly oxidized than glucose, such as pyruvate, is the precursor, there is no direct cytoplasmic source of NADH2. Quantitative data on the fate of pyruvate are in accord with the conclusion that the NADH2 was primarily formed intramitochondrially by the dehydrogenases of c ...
The Protein Product of the c-cb! Protooncogene Is Phosphorylated
... residues. The proline-rich region of p120'hI therefore provides a large number of potential binding sites for the SH3 domains of Btk and other proteins. The SH2 domains of Fyn, Lck, Blk, and other proteins have also been found to bind to p120~bl; however, these associations depend on the phosphoryla ...
... residues. The proline-rich region of p120'hI therefore provides a large number of potential binding sites for the SH3 domains of Btk and other proteins. The SH2 domains of Fyn, Lck, Blk, and other proteins have also been found to bind to p120~bl; however, these associations depend on the phosphoryla ...
Optical and Acoustic Detection of Laser
... • Can be spatially confined by tightly focusing laser beam • With femtosecond laser pulses, the generation threshold can be obtained with low total energy delivery (few nJ/pulse) • Virtually no collateral thermal and mechanical effects to out of focus areas • Laser generated bubbles can be clearly d ...
... • Can be spatially confined by tightly focusing laser beam • With femtosecond laser pulses, the generation threshold can be obtained with low total energy delivery (few nJ/pulse) • Virtually no collateral thermal and mechanical effects to out of focus areas • Laser generated bubbles can be clearly d ...
Shaping the Endoplasmic Reticulum into a Social Network
... In some family members[13_TD$IF], an APH is also the predicted N-terminal of the RHD. These RHDflanking elements provide additional stabilization. ER tubule formation does not solely rely on Rtns and Rtn-like proteins. In mammalian cells, tubules are constantly pulled out of the plane of ER membranes ...
... In some family members[13_TD$IF], an APH is also the predicted N-terminal of the RHD. These RHDflanking elements provide additional stabilization. ER tubule formation does not solely rely on Rtns and Rtn-like proteins. In mammalian cells, tubules are constantly pulled out of the plane of ER membranes ...
Distinct Cellular and Subcellular Patterns of Expression Imply
... similarity in structure and extensive colocalization, the specific function of each of these highly homologous proteins has been difficult to distinguish. A recent study in cultured cells indicates, however, that these proteins may have both unique and redundant functions in the establishment and m ...
... similarity in structure and extensive colocalization, the specific function of each of these highly homologous proteins has been difficult to distinguish. A recent study in cultured cells indicates, however, that these proteins may have both unique and redundant functions in the establishment and m ...
lysosomes
... • Occlusion of blood vessels decreases blood flow to the synovial tissue • This results in hypoxia and metabolic acidosis. • The reduction in pH results in the release of hydrolytic lysosomal enzymes from the synovial cells into the surrounding tissue • This initiates erosion of the articular cartil ...
... • Occlusion of blood vessels decreases blood flow to the synovial tissue • This results in hypoxia and metabolic acidosis. • The reduction in pH results in the release of hydrolytic lysosomal enzymes from the synovial cells into the surrounding tissue • This initiates erosion of the articular cartil ...
Minute Total Protein Extraction Kit (For Animal Cultured Cells and
... tissues is composed of optimized cell lysis buffer and protein extraction filter cartridges with 2.0 ml collection tubes. The kit is designed to rapidly extract total proteins from animal cells and tissues (invertebrate and vertebrate) for protein analysis and further purification. Since protein pro ...
... tissues is composed of optimized cell lysis buffer and protein extraction filter cartridges with 2.0 ml collection tubes. The kit is designed to rapidly extract total proteins from animal cells and tissues (invertebrate and vertebrate) for protein analysis and further purification. Since protein pro ...
Cells Review
... 1. Which of the following organelles is a mitchondria? a. A c. F b. C d. I 2. Which of the following organelles is a gogli body / apparatus? a. A c. C b. G d. H 3. Letter “L” is pointing to the a. Cell Wall c. Nuclear Membrane b. Cell Membrane d. Nuclear Pore 4. Which of the following organelle is t ...
... 1. Which of the following organelles is a mitchondria? a. A c. F b. C d. I 2. Which of the following organelles is a gogli body / apparatus? a. A c. C b. G d. H 3. Letter “L” is pointing to the a. Cell Wall c. Nuclear Membrane b. Cell Membrane d. Nuclear Pore 4. Which of the following organelle is t ...
Chapter 5 Gases - Rivermont Collegiate
... – Make and use the organic molecules of life – Consist of one or more cells – Engage in self-sustaining biological processes such as metabolism and homeostasis – Change over their lifetime, for example by growing, maturing, and aging – Use DNA as their hereditary material – Have the collective capac ...
... – Make and use the organic molecules of life – Consist of one or more cells – Engage in self-sustaining biological processes such as metabolism and homeostasis – Change over their lifetime, for example by growing, maturing, and aging – Use DNA as their hereditary material – Have the collective capac ...
Section 19-1 Bacteria
... a. The rod-shaped are called b. The spherical-shaped are called c. The corkscrew-shaped are called 17. A method of telling two different types of eubacteria apart by using dyes is called 18. What colors are Gram-positive and Gram-negative bacteria under the microscope when treated with Gram stain? 1 ...
... a. The rod-shaped are called b. The spherical-shaped are called c. The corkscrew-shaped are called 17. A method of telling two different types of eubacteria apart by using dyes is called 18. What colors are Gram-positive and Gram-negative bacteria under the microscope when treated with Gram stain? 1 ...
Adenovirus RIDα regulates endosome maturation by mimicking GTP
... complex encoded by E3 modulates trafficking of several cell surface receptors (Horwitz, 2004). RID is composed of two α subunits (formerly E3-13.7 or E3-10.4) and one β subunit (formerly E3-14.5). RIDα was initially discovered based on its ability to induce down-regulation of the EGF receptor (EGFR) ...
... complex encoded by E3 modulates trafficking of several cell surface receptors (Horwitz, 2004). RID is composed of two α subunits (formerly E3-13.7 or E3-10.4) and one β subunit (formerly E3-14.5). RIDα was initially discovered based on its ability to induce down-regulation of the EGF receptor (EGFR) ...
Endoplasmosis and exoplasmosis: the evolutionary principles
... spheres implies that the lipid bilayers, which form the membrane of organelles or the boundary between the cytoplasm and the extracellular environment, exhibit a plasmatic (or cis-) and an extraplasmatic (or trans-) face [3]. Endocytosis and exocytosis, as well as the membrane traffic within the cel ...
... spheres implies that the lipid bilayers, which form the membrane of organelles or the boundary between the cytoplasm and the extracellular environment, exhibit a plasmatic (or cis-) and an extraplasmatic (or trans-) face [3]. Endocytosis and exocytosis, as well as the membrane traffic within the cel ...
Biology 211 Anatomy & Physiology I
... While some neurons carry action potentials along their plasma membranes in this continuous fashion, most of them use a more efficient method of carrying action potentials called saltatory conduction. This is much more rapid and requires much less energy. ...
... While some neurons carry action potentials along their plasma membranes in this continuous fashion, most of them use a more efficient method of carrying action potentials called saltatory conduction. This is much more rapid and requires much less energy. ...
(PUNCH-P) reveals cell cycle
... newly synthesized proteins in cycling HeLa cells. Western blotting confirmed that nascent peptides were labeled and captured with high specificity and that components of the translation machinery were removed, as evidenced by the absence of ribosomal protein L26 from the streptavidin eluate (Fig. 3A ...
... newly synthesized proteins in cycling HeLa cells. Western blotting confirmed that nascent peptides were labeled and captured with high specificity and that components of the translation machinery were removed, as evidenced by the absence of ribosomal protein L26 from the streptavidin eluate (Fig. 3A ...
Cytosol
The cytosol or intracellular fluid (ICF) or cytoplasmic matrix is the liquid found inside cells. It is separated into compartments by membranes. For example, the mitochondrial matrix separates the mitochondrion into many compartments.In the eukaryotic cell, the cytosol is within the cell membrane and is part of the cytoplasm, which also comprises the mitochondria, plastids, and other organelles (but not their internal fluids and structures); the cell nucleus is separate. In prokaryotes, most of the chemical reactions of metabolism take place in the cytosol, while a few take place in membranes or in the periplasmic space. In eukaryotes, while many metabolic pathways still occur in the cytosol, others are contained within organelles.The cytosol is a complex mixture of substances dissolved in water. Although water forms the large majority of the cytosol, its structure and properties within cells is not well understood. The concentrations of ions such as sodium and potassium are different in the cytosol than in the extracellular fluid; these differences in ion levels are important in processes such as osmoregulation, cell signaling, and the generation of action potentials in excitable cells such as endocrine, nerve and muscle cells. The cytosol also contains large amounts of macromolecules, which can alter how molecules behave, through macromolecular crowding.Although it was once thought to be a simple solution of molecules, the cytosol has multiple levels of organization. These include concentration gradients of small molecules such as calcium, large complexes of enzymes that act together to carry out metabolic pathways, and protein complexes such as proteasomes and carboxysomes that enclose and separate parts of the cytosol.