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Cytoskeleton remodelling of confluent epithelial cells cultured on
Cytoskeleton remodelling of confluent epithelial cells cultured on

... cells. Numerous hard materials ranging from metals to silicon are used by the healthcare industry to serve as artificial joint replacements, stents or dental implants. Surface treatment to change the roughness has been proved to modulate adhesion of cells, cytokine release and gene expression of ost ...
AMBER PARTICLES AS LIVING PLANT CELL MARKERS IN FLOW
AMBER PARTICLES AS LIVING PLANT CELL MARKERS IN FLOW

... The tested plant cells reacted quite similarly to the amber components. The presence of amber components (alcohol soluble amber fraction and sodium succinate) in cultivation media mostly had no influence on cellular fluorescence. This indicates that the increase of cell fluorescence is not directly ...
Position Paper of the European Society of Cardiology Working
Position Paper of the European Society of Cardiology Working

... Pluripotent stem cells in clinical trials Another class among the second-generation cells are pluripotent stem cells, both ESCs and iPSCs (Table 2). Prerequisites for clinical application of PSCs for heart regeneration and repair is their efficient and strict differentiation into cardiomyocytes and ...
cell: the basic unit of structure in living things
cell: the basic unit of structure in living things

... cell has a different shape than a nerve cell. Skin cells have a different shape than fat cells. As you see, there are many differences amo ng cells. But cells also have many things in common. For example, all cells are made up of a living material called prot oplasm (PRO tub plaz urn). Protoplasm is ...
2.6 Isolation of trout head kidney and spleen leukocytes
2.6 Isolation of trout head kidney and spleen leukocytes

... develop into either DCs or macrophages. DCs are a heterogeneous population of cells that can be divided into two subpopulations, the myeloid and the lymphoid lineage. The myeloid lineage consists of precursors of Langerhans cells and interstitial DCs, while thymic DCs and plasmacytoid DCs belong to ...
Actin in plants
Actin in plants

... fact that cytoplasm re-arranged itself prior to division. Working on large, vacuolated cells induced to divide as a result of tissue wounding, they reported that cytoplasmic strands that radiated from the nucleus during interphase adopted a much simpler arrangement at prophase. Instead of radiating ...
Epithelial and Stromal Developmental Patterns in a Novel Substitute
Epithelial and Stromal Developmental Patterns in a Novel Substitute

... tissue [Hoath and Leahy, 2003]. Histologically, the epidermis mainly consists of a cell population of keratinocytes that express specific cytokeratins (CKs) that are responsible for many of the functions of the epithelium [Jacques et al., 2005; Proksch et al., 2008]. Numerous diseases and conditions ...
Cell Surface Membrane Remodelling and Mitochondrial
Cell Surface Membrane Remodelling and Mitochondrial

... [1,2], differentiated PC12 cells [4,13], sympathetic neurons [14], acute hippocampal slices [15, acute spinal cord slices [16], and hippocampal slice cultures [3]. 2.2 Optimizing imaging conditions To reduce complications associated with cell overlap, PC12 cells and primary neurons are plated at low ...
Differential Expression of Four Connexin Genes, Cx-26, Cx
Differential Expression of Four Connexin Genes, Cx-26, Cx

... of LH is its involvement, in concert with another gonadotropin, FSH, in follicular steroidogenesis. According to a proposed mechanism described in the two cell-two gonadotropin hypothesis (ll), an intercellular communication between two types of cells, granulosa and thecal cells, may be essential. T ...
Signaling pathways at the leading edge of chemotaxing cells
Signaling pathways at the leading edge of chemotaxing cells

... II, it regulates contraction of the uropod or posterior of the cell. Analysis of F-actin assembly is described in other reviews in this volume. Myosin I and PAKc preferentially localize to the leading edge and are essential for proper chemotaxis. Myosin II disassembly at the leading edge is mediated ...
Chapter 12 - Blood 12.1 Introduction (p. 322) A. Blood is considered
Chapter 12 - Blood 12.1 Introduction (p. 322) A. Blood is considered

... This reflex lasts only a few minutes, but it lasts long enough to initiate the second and third steps of hemostasis. C. Platelet Plug Formation (p. 333; Fig. 12.12) ...
Cell Cycle-dependent Cytotoxicity of Alkylating
Cell Cycle-dependent Cytotoxicity of Alkylating

... mid to late S phase are the most resistant. The ultimate poten tial for clinically exploiting such characteristics depends on a more complete understanding of the relationships between these variations in cytotoxicity and biochemical effects of the drug in the different phases of the cell cycle. DNA ...
cell cycle phase expansion in nitrogen
cell cycle phase expansion in nitrogen

... Expansion of Cell Cycle Phases in Yeast ...
Chapter 14
Chapter 14

... This reflex lasts only a few minutes, but it lasts long enough to initiate the second and third steps of hemostasis. C. Platelet Plug Formation (p. 333; Fig. 12.12) ...
Chapter 6 A Tour of the Cell
Chapter 6 A Tour of the Cell

...  Microfilaments that function in cellular motility contain the protein myosin in addition to actin  In muscle cells, thousands of actin filaments are arranged parallel to one another  Thicker filaments composed of myosin interdigitate with the ...
Disease of Mechanisms of Fungi
Disease of Mechanisms of Fungi

... living host. These fungi possess greater invasive properties than those causing superficial infections, but they are limited to the keratinized tissues. They cause a wide spectrum of diseases that range from a mild scaling disorder to one that is generalized and highly inflammatory. Studies have sho ...
Antifolate Polyglutamylation and Competitive Drug Displacement at
Antifolate Polyglutamylation and Competitive Drug Displacement at

... Previous studies from this laboratory have shown that the addition of leucovorin to tumor cells dissociates methotrexate, but not methotrexate polyglutamates, from dihydrofolate reduc Ã-ase(L. H. Matherly, D. W. Fry, and I. D. Goldman, Cancer Res., 43: 2694-2699, 1983). To further assess the importa ...
Glucosamine induces cell-cycle arrest and hypertrophy of mesangial
Glucosamine induces cell-cycle arrest and hypertrophy of mesangial

... matrix recognition through interactions with adhesion receptors such as integrins and matrix proteins (collagen and fibronectin) or with other glycosphingolipids [19]. On the other hand, gangliosides are known to regulate transmembrane signalling by modulating functional membrane proteins. Thus they ...
Rearrangement of the Keratin Cytoskeleton after
Rearrangement of the Keratin Cytoskeleton after

... no biochemical inhibitors analogous to cytostatic drugs used to destabilize microtubules or microfilaments have been found that specifically interfere with cytokeratin structure or ...
Metabolism and Digestion-Lecture 4
Metabolism and Digestion-Lecture 4

... • Mutations in CFTR chloride channel gene • Although lung disease most widely known correlate, named for characteristic pancreatic lesions • Inability to secrete bicarbonate from pancreas • Pancreas is not flushed. In duodenum, acid remains unneutralised and pancreatic enzymes remain inactive. • In ...
differentiation and proliferation of embryonic mast
differentiation and proliferation of embryonic mast

... cell development in rat embryos. Mast cells arise ubiquitously in and are confined to the loose connective tissue in the embryo. The alcian blue--safranin reaction distinguishes between weakly sulfated and strongly sulfated mucopolysaccharides by a shift from alcian blue to safranin staining. Based ...
Phosphorylation Controls CLIMP-63–mediated Anchoring of the
Phosphorylation Controls CLIMP-63–mediated Anchoring of the

... is mainly involved in the positioning of endosomes, lysosomes, and the Golgi apparatus (Goodson et al., 1997; Hirokawa, 1998). Dynein-based movement of ER membranes also has been observed in some cases but seems to occur less frequently (Allan, 1995; Lane and Allan, 1999; WedlichSoldner et al., 2002 ...
Mesenchymal stem cell exosome: a novel stem cell
Mesenchymal stem cell exosome: a novel stem cell

... Compared with other secreted vesicles, exosomes have much better defined biophysical and biochemical properties(reviewed in [64]). They have a diameter of 40–100 nm, with a density in sucrose of 1.13–1.19 g/ml, and can be sedimented at 100,000 g. Their membranes are enriched in cholesterol, sphingom ...
Functional Complexity Associated with the EspB Molecule of
Functional Complexity Associated with the EspB Molecule of

... Human red blood cells (Type B) were washed with PBS three times and suspended in PBS to a final concentration of 3% (v/v). RBC was then plated on wells of 12-well plates (700 l/well) coated with 1% poly-lysine for 20 min at 37 C followed by two PBS washes and incubated in DMEM medium without pheno ...
Shininger, AnnRev. Plant Physiol. 1979 30:313-37
Shininger, AnnRev. Plant Physiol. 1979 30:313-37

... developmentunless specifically altered by influences from developing leaves. Theinterpretationof the histologicalpreparationsis clearly a subjective matter since one does not knowfrom these kinds of experimental approacheswhether the cells are committedto vascular development,to another fate, or not ...
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Tissue engineering



Tissue engineering is the use of a combination of cells, engineering and materials methods, and suitable biochemical and physicochemical factors to improve or replace biological functions. While it was once categorized as a sub-field of biomaterials, having grown in scope and importance it can be considered as a field in its own right.While most definitions of tissue engineering cover a broad range of applications, in practice the term is closely associated with applications that repair or replace portions of or whole tissues (i.e., bone, cartilage, blood vessels, bladder, skin, muscle etc.). Often, the tissues involved require certain mechanical and structural properties for proper functioning. The term has also been applied to efforts to perform specific biochemical functions using cells within an artificially-created support system (e.g. an artificial pancreas, or a bio artificial liver). The term regenerative medicine is often used synonymously with tissue engineering, although those involved in regenerative medicine place more emphasis on the use of stem cells or progenitor cells to produce tissues.
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