Pancreatic Beta Cell Lines and their Applications in Diabetes
... Various transformation approaches have been used in the last 30 years to overcome replicative senescence, including induction of pancreatic tumours by irradiation (Gazdar et al., 1980) or viral infection (Santerre et al., 1981), immortalisation of beta cells in vitro and development of transgenic mi ...
... Various transformation approaches have been used in the last 30 years to overcome replicative senescence, including induction of pancreatic tumours by irradiation (Gazdar et al., 1980) or viral infection (Santerre et al., 1981), immortalisation of beta cells in vitro and development of transgenic mi ...
Centrosome Biology: A SAS-sy Centriole in the Cell Cycle Dispatch
... because it is the γ-TuRC and other components of the pericentriolar material that nucleate and anchor microtubules. It should be noted that eukaryotes present a continuum of microtubule organization strategies. In yeast and other fungi, all microtubules grow from highly ordered centrosome-like organ ...
... because it is the γ-TuRC and other components of the pericentriolar material that nucleate and anchor microtubules. It should be noted that eukaryotes present a continuum of microtubule organization strategies. In yeast and other fungi, all microtubules grow from highly ordered centrosome-like organ ...
Is the shoot a root with a view? Philip N Benfey
... Because previous work pointed to TTG as the prime regulator of shoot organ-specificity, it has been suggested that overexpression of GL1 might induce TTG in these organs [25••]. Nevertheless, in both of the GL1 overexpression situations (with or without mutant tryptichon), no alteration in root hair ...
... Because previous work pointed to TTG as the prime regulator of shoot organ-specificity, it has been suggested that overexpression of GL1 might induce TTG in these organs [25••]. Nevertheless, in both of the GL1 overexpression situations (with or without mutant tryptichon), no alteration in root hair ...
Control of Mitotic Events by Nap1 and the Gin4 Kinase
... kinase complexes with specificity. To learn more about how cyclins might function in this capacity, we used affinity chromatography to identify proteins that interact with one kind of cyclin, but not with others. We reasoned that such proteins would be likely to play a role in the specific cell cycl ...
... kinase complexes with specificity. To learn more about how cyclins might function in this capacity, we used affinity chromatography to identify proteins that interact with one kind of cyclin, but not with others. We reasoned that such proteins would be likely to play a role in the specific cell cycl ...
auxin
... No one can look at the plants growing on a bank or on the borders of a thick wood, and doubt that the young stems and leaves place themselves so that the leaves may be well illuminated...they are extremely heliotropic; and this probably serves...as a guide (for) the buried seeds through fissures in ...
... No one can look at the plants growing on a bank or on the borders of a thick wood, and doubt that the young stems and leaves place themselves so that the leaves may be well illuminated...they are extremely heliotropic; and this probably serves...as a guide (for) the buried seeds through fissures in ...
The added value of single-cell gene expression
... consists of about 300 000 mRNA molecules represented by some 10 000 expressed genes [26]. Some genes are highly expressed (>100 transcripts per cell), but most genes have a mean expression of less than one molecule per cell. In the bacteria Mycoplasma pneumoniae, the mean mRNA count was determined t ...
... consists of about 300 000 mRNA molecules represented by some 10 000 expressed genes [26]. Some genes are highly expressed (>100 transcripts per cell), but most genes have a mean expression of less than one molecule per cell. In the bacteria Mycoplasma pneumoniae, the mean mRNA count was determined t ...
In Vivo Assembly of Phage 29 Replication Protein p1 into
... viral-encoded proteins (11). Thus, viral proteins appear to be involved in the attachment of 29 DNA to the bacterial membrane. The genome of 29 is a linear double-stranded DNA molecule with a terminal protein (TP)1 covalently linked at each 5⬘-end. Its replication starts at either DNA end, where t ...
... viral-encoded proteins (11). Thus, viral proteins appear to be involved in the attachment of 29 DNA to the bacterial membrane. The genome of 29 is a linear double-stranded DNA molecule with a terminal protein (TP)1 covalently linked at each 5⬘-end. Its replication starts at either DNA end, where t ...
Supplementation of Animal Cell Culture Media
... Animal blood serum is by far the most common supplement to culture medium. It provides necessary proteins, growth factors, hormones, lipids, vitamins, attachment factors, and trace metals for culturing cells in leaner classical media formulations. Because serum has been used in cell culture for so l ...
... Animal blood serum is by far the most common supplement to culture medium. It provides necessary proteins, growth factors, hormones, lipids, vitamins, attachment factors, and trace metals for culturing cells in leaner classical media formulations. Because serum has been used in cell culture for so l ...
MOVEMENT AND GUIDANCE OF MIGRATING MESODERMAL
... active and rapid locomotion in vitro when dissociated and cultured in adequate conditions (Nakatsuji & Johnson, 1982). Thus, at present, we can identify two major forces that appear to be essential in gastrulation: (1) the migration of the mesodermal cells on the inner surface of the ectodermal laye ...
... active and rapid locomotion in vitro when dissociated and cultured in adequate conditions (Nakatsuji & Johnson, 1982). Thus, at present, we can identify two major forces that appear to be essential in gastrulation: (1) the migration of the mesodermal cells on the inner surface of the ectodermal laye ...
Solar Cell
... Figure 6. (a) Close up of a screen used for printing the front contact of a solar cell. During printing, metal paste is forced through the wire mesh in unmasked areas. The size of the wire mesh determines the minimum width of the fingers. Finger widths are typically 100 to 200 µm(b) Close up of a fi ...
... Figure 6. (a) Close up of a screen used for printing the front contact of a solar cell. During printing, metal paste is forced through the wire mesh in unmasked areas. The size of the wire mesh determines the minimum width of the fingers. Finger widths are typically 100 to 200 µm(b) Close up of a fi ...
think!
... Cell Structure: Slime molds are multicellular mass when they are clumped together as seen above. They are beautifully colored with many being a bright yellow. ...
... Cell Structure: Slime molds are multicellular mass when they are clumped together as seen above. They are beautifully colored with many being a bright yellow. ...
Chapter_12
... propagation delay, switching delay Assume propagation at two-thirds speed of light If source and destination on opposite sides of USA, propagation time ~ 48x10-3 seconds Given implicit congestion control, by the time dropped cell notification has reached source, 7.2x106 bits have been transmitted ...
... propagation delay, switching delay Assume propagation at two-thirds speed of light If source and destination on opposite sides of USA, propagation time ~ 48x10-3 seconds Given implicit congestion control, by the time dropped cell notification has reached source, 7.2x106 bits have been transmitted ...
Biology Lesson Plans - Paint Valley Local Schools
... HW: Study for Ch. 8 Test. (The test will be Monday, November 25, 2013.) Science Inquiry and Application During the years of grades 9 through 12, all students must use the following scientific processes with appropriate laboratory safety techniques to construct their knowledge and understanding in al ...
... HW: Study for Ch. 8 Test. (The test will be Monday, November 25, 2013.) Science Inquiry and Application During the years of grades 9 through 12, all students must use the following scientific processes with appropriate laboratory safety techniques to construct their knowledge and understanding in al ...
Knocking on the heaven`s wall: pathogenesis of and resistance to
... enhanced by up to seven-fold and allow the subsequent differentiation of haustorial complexes in leaf epidermal cells. However, the further growth of the inappropriate fungus is terminated in each pen mutant coincident with a cell death response of attacked epidermal cells. This cell death is remini ...
... enhanced by up to seven-fold and allow the subsequent differentiation of haustorial complexes in leaf epidermal cells. However, the further growth of the inappropriate fungus is terminated in each pen mutant coincident with a cell death response of attacked epidermal cells. This cell death is remini ...
Characterization of Dependencies Between Growth and
... Cell division is a process fundamental to all life, and dysregulation of the process is common in diseases like cancer. Since it underlies so many biological phenomena, cell division is highly coordinated with other cellular processes. For instance, in the budding yeast Saccharomyces cerevisiae, cel ...
... Cell division is a process fundamental to all life, and dysregulation of the process is common in diseases like cancer. Since it underlies so many biological phenomena, cell division is highly coordinated with other cellular processes. For instance, in the budding yeast Saccharomyces cerevisiae, cel ...
Life under pressure: hydrostatic pressure in cell growth and function
... properties of water not only promoted the emergence of cellular life but also set limits on effective cell dimensions within which viability and reproduction can be maintained [1]. It was crucial for cell function that osmolality, membrane tension and hydrostatic pressure (see Glossary) were tightly ...
... properties of water not only promoted the emergence of cellular life but also set limits on effective cell dimensions within which viability and reproduction can be maintained [1]. It was crucial for cell function that osmolality, membrane tension and hydrostatic pressure (see Glossary) were tightly ...
Photobleaching Substrates Characterized Using Fluorescence
... Ku-GFP molecules with that of the heterodimer is illustrated by the merge images where the overlap is indicated by yellow. Fig. 1 shows that both Ku70- and Ku86-GFP efficiently associate with endogenous protein and are therefore faithful reporters of endogenous Ku. Ku70- and Ku86-GFP are highly mobi ...
... Ku-GFP molecules with that of the heterodimer is illustrated by the merge images where the overlap is indicated by yellow. Fig. 1 shows that both Ku70- and Ku86-GFP efficiently associate with endogenous protein and are therefore faithful reporters of endogenous Ku. Ku70- and Ku86-GFP are highly mobi ...
Decision of Spindle Poles and Division Plane by Double
... to play an important role in cytokinesis of plant cells. In particular, the actin-depleted zone (ADZ) was identified as the region where the cortical MFs disappeared from the division site at the equator of the cell after disappearance of the PPB-like bands of MFs in Tradescantia stamen hair cells ( ...
... to play an important role in cytokinesis of plant cells. In particular, the actin-depleted zone (ADZ) was identified as the region where the cortical MFs disappeared from the division site at the equator of the cell after disappearance of the PPB-like bands of MFs in Tradescantia stamen hair cells ( ...
Differential Sperm Cell Gene Expression in Plumbago
... generative cells, which are the immediate precursors to sperm cells. Work on cells of the male germ linage was impeded by problems in obtaining sufficient sperm cells to conduct molecular biology and in maintaining their purity during isolation from the pollen2. At that time, the accepted paradigm w ...
... generative cells, which are the immediate precursors to sperm cells. Work on cells of the male germ linage was impeded by problems in obtaining sufficient sperm cells to conduct molecular biology and in maintaining their purity during isolation from the pollen2. At that time, the accepted paradigm w ...
Standard PDF - Wiley Online Library
... of actin filaments with AJs (C, D). Arrows show actin filament orientations. In the punctum adherens (PA), actin filament bundles are associated with the plasma membrane perpendicularly (A, C). NRK cells are stained for P-cadherin (magenta) showing the position of PAs and actin filaments (green). In ...
... of actin filaments with AJs (C, D). Arrows show actin filament orientations. In the punctum adherens (PA), actin filament bundles are associated with the plasma membrane perpendicularly (A, C). NRK cells are stained for P-cadherin (magenta) showing the position of PAs and actin filaments (green). In ...
Negative Controls of Cell Proliferation: Human
... LNCaP cells represent a useful tool to explore the mechanism of sex hormone action on cell proliferation in an "in culture-in animal" model. Results indicated that: (a) these cells were inhibited from proliferating for extended periods (up to 30 days) when placed in charcoal-dextranstripped sera; th ...
... LNCaP cells represent a useful tool to explore the mechanism of sex hormone action on cell proliferation in an "in culture-in animal" model. Results indicated that: (a) these cells were inhibited from proliferating for extended periods (up to 30 days) when placed in charcoal-dextranstripped sera; th ...
Lineage Commitment During T cell Development
... How would you test the importance of one of the transcription factors (for instance, TCF-1) that is upregulated during early Notch signaling for T cell lineage development? ...
... How would you test the importance of one of the transcription factors (for instance, TCF-1) that is upregulated during early Notch signaling for T cell lineage development? ...
Molecular Mechanisms of Transforming Growth Factor
... Five TGF-b ligands have been cloned (TGF-b1–5). These ligands are secreted as 100-kDa inactive complexes. The inactive form consists of a dimer of the N-terminal peptide, noncovalently associated with the 25-kDa dimer of the biologically active form. Structurally, the ligands exhibit a high degree o ...
... Five TGF-b ligands have been cloned (TGF-b1–5). These ligands are secreted as 100-kDa inactive complexes. The inactive form consists of a dimer of the N-terminal peptide, noncovalently associated with the 25-kDa dimer of the biologically active form. Structurally, the ligands exhibit a high degree o ...
Cell cycle
The cell cycle or cell-division cycle is the series of events that take place in a cell leading to its division and duplication (replication) that produces two daughter cells. In prokaryotes which lack a cell nucleus, the cell cycle occurs via a process termed binary fission. In cells with a nucleus, as in eukaryotes, the cell cycle can be divided into three periods: interphase, the mitotic (M) phase, and cytokinesis. During interphase, the cell grows, accumulating nutrients needed for mitosis, preparing it for cell division and duplicating its DNA. During the mitotic phase, the cell splits itself into two distinct daughter cells. During the final stage, cytokinesis, the new cell is completely divided. To ensure the proper division of the cell, there are control mechanisms known as cell cycle checkpoints.The cell-division cycle is a vital process by which a single-celled fertilized egg develops into a mature organism, as well as the process by which hair, skin, blood cells, and some internal organs are renewed. After cell division, each of the daughter cells begin the interphase of a new cycle. Although the various stages of interphase are not usually morphologically distinguishable, each phase of the cell cycle has a distinct set of specialized biochemical processes that prepare the cell for initiation of cell division.