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Automated Staining of Pluripotent Cells with Tra-1-60 and
Automated Staining of Pluripotent Cells with Tra-1-60 and

... Human iPSCs were routinely passaged with 0.5 µM EDTA and maintained with Essential 8™ medium from Invitrogen on Matrigel™ (1:80 in DPBS). iPSCs were then freshly dissociated into single-cell suspension using Accutase for 5-7 minutes at 37˚C. Human NPCs were differentiated from iPSCs in chemically de ...
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Name______Answer Key__________________

... Saturated fatty acid have the most amount of hydrogen atoms that they can possibly have. So they are saturated with hydrogen. 3. Why do saturated fatty acids have more of tendency to clog arteries than unsaturated fats? The molecular structure of saturated fatty acids is more condensed. There is les ...
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the journal of cell biology - Murphy Lab

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... the target of the bacteriocin. In fact, some differences are observed with respect to E. coli ftsI mutants. For example, treated L. lactis cells do not form long filaments, although they suffer an elongation that precedes macromolecular synthesis arrest and death of the cell. This might be due to th ...
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... substances to enter and keeps some substances out. This is important for all life processes. A diagram of cell membrane structure is below. ...
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Organelles - Brookville Local Schools

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patriciazuk.com

... the cell cycle • internal signal – e.g. kinetochores not attached to spindle microtubules send a molecular signal that delays anaphase – all chromosomes must be attached to the spindle in order to eventually activate and enzyme called separase – separase breaks down the cohesin proteins within the c ...
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3.1 Cell Theory

... groundwork for all biological research that followed. However, it had to be refined over the years as additional data led to new conclusions. For example, Schwann stated in his publication that cells form spontaneously by free-cell formation. As later scientists studied the process of cell division, ...
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... its benzyl guanine substrates and to remove its affinity for DNA. In mammalian cells, SNAP-tag localizes to the cytoplasm and the nucleus. 2. How does it work? The SNAP-tag is a protein tag that forms a highly stable, covalent thioether bond with fluorophores or other substituted groups when appende ...
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Cell growth

The term cell growth is used in the contexts of cell development and cell division (reproduction). When used in the context of cell division, it refers to growth of cell populations, where a cell, known as the ""mother cell"", grows and divides to produce two ""daughter cells"" (M phase). When used in the context of cell development, the term refers to increase in cytoplasmic and organelle volume (G1 phase), as well as increase in genetic material (G2 phase) following the replication during S phase.
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