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Tissue
Tissue

... Exocrine gland (exo-outside + krino-to separate): glands with ducts; secretions pass through ducts onto a surface or into an organ ...
Chapter 6 Biology AP Notes
Chapter 6 Biology AP Notes

... Cell fractionation prepares isolates of specific cell components so that the functions of these organelles can be determined, especially by the reactions or processes catalyzed by their proteins. ○ For example, one cellular fraction was enriched in enzymes that function in cellular respiration. ○ El ...
Document
Document

... There are 2 main groups: Gram positive and Gram negative. Gram staining is a differential staining technique that provides an easy differentiation of bacteria into one of two groups. The staining technique, developed in the late 1700’s by Christian Gram classifies the rigid cell walled bacteria into ...
Cell Transport Notes
Cell Transport Notes

... Hypertonic: The solution has a higher concentration of solutes and a lower concentration of water than inside the cell. inside cell = ↑ [water] ...
FACS Procedure
FACS Procedure

... The day prior to transfection, cells will be prepared for you at a density of 0.5 to 1 X 105 cells/well in each well of a 6 well plate. ...
Embryonic electronics - The Department of Computer Science
Embryonic electronics - The Department of Computer Science

... There are four basic cell activities: multiplication, movement, change in character, and signaling (Wolpert, 1991, p. 7; Wolpert et al., 1998). In a silicon substrate, we demonstrate that it is possible to create artificial cells, exhibiting three of the above properties: cellular division (multipli ...
fundamental unit of life biology
fundamental unit of life biology

... NUCLEUS:Nucleus is a dense protoplasmic body that contains hereditary information for controlling cell activities as well as for transfer to next generation. It is the largest cell structure which is oval or spherical in outline. Nucleus lies in median or central position in animal and young plant c ...
Physical properties of the fluid should be noted, such as volume
Physical properties of the fluid should be noted, such as volume

... formation if not in EDTA, and odor. Total protein by refractometry should be determined by using the plasma protein scale of the refractometer. While specific gravity may be measured on the urine specific gravity scale, it does not add to information other than as an index of protein content. Total ...
A novel checkpoint mechanism regulating the G1/S transition
A novel checkpoint mechanism regulating the G1/S transition

... to be synthesized de novo before every S phase, in a Cdc10-dependent process (Kelly et al. 1993; Hofmann and Beach 1994; Nishitani et al. 2000). Immunoblotting showed that the production of Cdc18 (Fig. 2A) and Cdt1 (Fig. 2B) was not significantly delayed or reduced by UV irradiation. In fact, the le ...
Peer-reviewed Article PDF
Peer-reviewed Article PDF

... In contrast, due to immobile nature of plants, they cannot escape from adverse environmental conditions including high light, high temperature, chilling, drought, ultra-violet radiation during their consecutive stages of development [1-4]. Plants have evolved highly organized strategies to cope with ...
APOPTOSIS: An overview
APOPTOSIS: An overview

... DNA fragmentation followed by rapid engulfment of corpses by neighbouring cells. (Tommi, 2002) Essential part of life for every multicellular organism from worms to humans. (Faddy et al.,1992) ...
APOPTOSIS: An overview
APOPTOSIS: An overview

... DNA fragmentation followed by rapid engulfment of corpses by neighbouring cells. (Tommi, 2002) Essential part of life for every multicellular organism from worms to humans. (Faddy et al.,1992) ...
Foundations
Foundations

... General characteristics Amacrine cell circuitry as revealed by EM A2: a narrow-field, cone pathway amacrine cell AII: a bistratified rod amacrine cell A8: a bistratified cone amacrine cell A13: a small-field amacrine cell of the cone system A17: the wide-field reciprocal rod amacrine cell A19 and A2 ...
Document
Document

... *Many spinous processes are to be found on the cell surface. *The cytoplasm is basophilic. ...
Chapter 5 - Phillips Scientific Methods
Chapter 5 - Phillips Scientific Methods

... 16. IN A HYPOTONIC SOLUTION, THE CONCENTRATION OF SOLUTE MOLECULES OUTSIDE THE CELL IS LOWER THAN THE CONCENTRATION OF SOLUTES INSIDE THE CELL CYTOSOL. 17. In Hypotonic Solutions, WATER DIFFUSES INTO THE CELL until Equilibrium is established. 18. In Hypotonic Solutions, Water flows into the Cell bec ...
Active Transport
Active Transport

... molecules is to equally distribute themselves on either side of a membrane. However, by spending some energy to push the boulder higher and higher, you have the potential to use the boulder to do useful work that would be impossible otherwise. The same is true for molecules. ...
Supporting Information Fredericks et al. 10.1073/pnas.1417176111
Supporting Information Fredericks et al. 10.1073/pnas.1417176111

... obtained from Sigma, unless otherwise noted. MCP-1 (100 ng/mL) was used to stimulate macrophages as previously described (1) and immune complexes composed of 1.0 μm polystyrene beads (Invitrogen) coated with BSA (Invitrogen) and opsonized with antiBSA IgG (Millipore) have been previously described ( ...
Tailor Made Protein Synthesis for HSCs - Barna Lab
Tailor Made Protein Synthesis for HSCs - Barna Lab

... total RNA content. Moreover, even forced entry into the cell cycle does not appear to revert this shutdown of protein synthesis. These studies however do not exclude the possibility that a shutdown in protein synthesis is simply a reflection of HSC quiescence compared to other progenitor lineages. I ...
Notes
Notes

... A group of different kinds of tissues that coordinate their actions into a main primary function is called an organ. A group of organs and tissues that work together to maintain homeostasis in the body are called a system. ...
Preliminary evidence of a new microbial species capable of
Preliminary evidence of a new microbial species capable of

... Second primer set PCR product about of ~ 1400 base pairs in size is shown in Table 2. The resulting sequence was checked for similarity to other known sequences using NCNI’s BLAST and Ribosomal Database Project (RDP). The sequence shared 99% similarity with 16S rDNA gene sequence of an uncultured or ...
Heritable Stochastic Switching Revealed by Single-Cell Genealogy
Heritable Stochastic Switching Revealed by Single-Cell Genealogy

... position effect in yeast [3] and mouse [4], and prions such as Psiþ in yeast [5]. The time scale over which epigenetic phenotypes may persist spans many orders of magnitude and depends strongly on the physical mechanism used by the cell [6]. In general, however, epigenetic phenotypes are substantial ...
Chapter 5
Chapter 5

... – Consists of cells that are long and narrow (threadlike), have alternating light and dark cross-markings called striations, and many nuclei (multinucleate) – When stimulated by a nerve cell, protein filaments within the muscle cell slide past one another causing the muscle cell to contract • Skelet ...
Cells and Their Environment
Cells and Their Environment

... 3 Types of Diffusion • 1. Diffusion (simple) is the movement of molecules from an area of higher concentration to an area of lower concentration. • Small molecules can pass through the cell membrane by diffusion • Diffusion across a membrane is a type of passive transport because it does not requir ...
Intro Neurology
Intro Neurology

... channels than Na+, and this helps to create the electrochemical gradients that exist. Facilitated diffusion. 5. Sodium-potassium exchange pump- pumps 3 Na+ out and 2 K+ in; constantly working; helps to establish and maintain the electrochemical gradients that exist. Active transport. B. The nerve ce ...
Cytotoxicity of resin monomers on human gingival - GCP
Cytotoxicity of resin monomers on human gingival - GCP

... The Alamar Blue assay incorporates a fluorometric/colorimetric growth indicator based on detection of metabolic activity. The system incorporates an oxidation–reduction indicator that both fluoresces and changes colour in response to chemical reduction of growth medium resulting from cell growth [8]. ...
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Cell culture



Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.
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