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Sensing of Osmotic Pressure Changes in Tomato Cells
Sensing of Osmotic Pressure Changes in Tomato Cells

Data S1.
Data S1.

... calculated using the following formula: Enrichment factor = absorbance of the sample (treated heart) / absorbance of the control (untreated heart). -quantitation of caspase-3 activity; whole hearts from untreated and H/R adult zebrafish were homogenized (TissueLyser, Qiagen) in Laemmli buffer (100 m ...
Complementary action of the PGC-1 coactivators in mitochondrial
Complementary action of the PGC-1 coactivators in mitochondrial

... cells had an equivalent UCP1 protein level to wt cells under basal condition, but no increase in UCP1 protein expression was observed after dbcAMP treatment. The induction of several mRNAs encoded by other genes involved in thermogenesis was either severely impaired in KO cells (C/EBPb) or was even ...
computer simulation of a living cell: part i
computer simulation of a living cell: part i

... Garfinkel, 1966) and to study repression or induction of one gene (Griffith, 1968). Garfinkel has simulated the glycolytic pathway in elegant detail for higher protists. Heinmetz (1964) has solved differential equations on an analog computer in order to study repression of one enzyme. I am synthesis ...
1. Which phrase is an example of autotrophic
1. Which phrase is an example of autotrophic

... as they photosynthesize while feeding on the coral’s waste. Extremely sensitive, corals survive in a narrow range of temperature, sunlight and salinity. An uncommonly severe El Niño in 1998 raised ocean temperatures and changed currents, causing bleaching that devastated reefs worldwide. Scientists ...
PDF - Oxford Academic - Oxford University Press
PDF - Oxford Academic - Oxford University Press

... Regardless of whether multicellularity reflects the direct consequence of natural selection, cladistic analyses reveal that its acquisition releases organisms from functioning as gametes (which is the fate of most unicellular eukaryotes), lengthens the diploid phase in haploid–diploid life cycles (t ...
Materials - Web Adventures
Materials - Web Adventures

... Prion – (pronounced PREE-on) An infectious agent made only of proteins. Prions cause Bovine Spongiform Encephalopathy (Mad Cow) in cows and variant Creutzfeldt-Jakob disease (vCJD) in humans. The disease is spread through abnormal proteins that cause other normal proteins to change to the prion's ab ...
Measuring the mechanical properties of plant cells by combining
Measuring the mechanical properties of plant cells by combining

... find model parameters that best fit the data. Several models have been proposed that describe indentation experiments at different scales. A  mathematical model that is often used to interpret data from AFM experiments is the Hertz model (Lin et al., 2007). This assumes that indentations are small e ...
Cell Cycle Phase Specificity and Biochemical
Cell Cycle Phase Specificity and Biochemical

... the drug. Parts of this study were previously reported (2). MATERIALS AND METHODS Cell Culture. DON cells, a Chinese hamster fibroblast line (ATCC CCL16), were grown at 37°in McCoy's Medium 5a, modified by the addition of lactalbumin hydrolysate (0.8 g/liter) and fetal calf serum (200 ml/liter). Th ...
Evidence for a non-replicative intracellular stage of
Evidence for a non-replicative intracellular stage of

... Nontypable Haemophilus influenzae (NTHi) is a Gram-negative, non-capsulated human bacterial pathogen, a major cause of a repertoire of respiratory infections, and intimately associated with persistent lung bacterial colonization in patients suffering from chronic obstructive pulmonary disease (COPD) ...
Germinal Center Dynamics Revealed by Multiphoton Microscopy with a Photoactivatable Fluorescent Reporter
Germinal Center Dynamics Revealed by Multiphoton Microscopy with a Photoactivatable Fluorescent Reporter

... of CD40 and BCR ligation (Basso et al., 2004; Zhu et al., 2004). This analysis showed the LZ population to be strongly imprinted with the signatures of both of these activation signals (Figure 3D and Table S3). Taken together, our cell-cycle and gene expression analyses support the notion that cells ...
Cell Death and Differentiation
Cell Death and Differentiation

... doi:10.1038/sj.cdd.4401712; published online 8 July 2005 Keywords: calcium; calmodulin; CaM-binding protein; IQ motif; BAG domain; stress; Arabidopsis Abbreviations: CaM, calmodulin; CaMBPs, CaM-binding proteins; BAG, BCL-2-associated athanogene; AtBAG, Arabidopsis thaliana BAG; CDD, cell death doma ...
Gene Section GPC5 (glypican 5)  Atlas of Genetics and Cytogenetics
Gene Section GPC5 (glypican 5) Atlas of Genetics and Cytogenetics

... The precise functions of GPC5 have yet to be fully established. HSPGs are common constituents of cell surfaces and the extracellular matrix (ECM), with essential functions in cell growth and development (Burgess and Macaig, 1989; Andres et al., 1992). Glypicans appear to be expressed predominantly d ...
Fast lysis of Escherichia coli filament cells requires
Fast lysis of Escherichia coli filament cells requires

Epithelial reticular Cells of Egyptian Water buffalo (Bosbubalis)
Epithelial reticular Cells of Egyptian Water buffalo (Bosbubalis)

... cattle and water buffalo were differently respond to certain infectious agents and existed in very different environments, it was likely that the water buffalo would not be affected by vaccines and management regimens designed for cattle[3]. It was important to characterize the immune system of the ...
Accurate cell segmentation in microscopy images using membrane
Accurate cell segmentation in microscopy images using membrane

... Associate Editor: Jonathan Wren ...
Biology Unit - The Connected Hub
Biology Unit - The Connected Hub

... _______ (a carbohydrate) down into glucose: Protease (produced in the stomach, pancreas and small intestine) breaks _______ down into amino acids: Lipase (produced in the pancreas and small intestine) breaks fats (_____) down into fatty acids and glycerol: ...
Identification of Motile Sperm Domain–Containing Protein 2 as
Identification of Motile Sperm Domain–Containing Protein 2 as

... FIGURE 3. MOSPD2 promotes monocytic line migration. (A) mRNA and protein expression of MOSPD2 in U937 cells transduced with sh-Control or sh-MOSPD2 #1 lentiviral particles. 18S was used to normalize RNA levels. Samples were run in triplicate. One of at least three experiments is shown. (B) Transwell ...
Lisa
Lisa

... It supports the body to stand up and supports soft tissues. It’s the framework of the body. Gives muscles something to attach to, so it helps movement. Bones are the things that move when muscles contract. Bones can act as levers. -to protect internal organs from damage. -bones store and release che ...
NCEA Level 1 Biology (90927) 2014
NCEA Level 1 Biology (90927) 2014

... Viruses are also difficult to treat using antibiotics as they are ineffective on viruses, and can be used to treat only bacteria infections as they target and work on destroying the bacteria’s cell wall. Because viruses do not have a cell wall and are not living cells, this method of treatment is no ...
111KB - NZQA
111KB - NZQA

... Viruses are also difficult to treat using antibiotics as they are ineffective on viruses, and can be used to treat only bacteria infections as they target and work on destroying the bacteria’s cell wall. Because viruses do not have a cell wall and are not living cells, this method of treatment is no ...
STK31 maintains the undifferentiated state of colon cancer cells
STK31 maintains the undifferentiated state of colon cancer cells

... of STK31 by real time-PCR. The results showed that the expression of STK31 transcripts were significantly increased in 5 out of 8 patients (P < 0.05) compared with normal tissue (n = 8; Figure 1B), confirming the reactivation of STK31 in colon cancer. We next examined the expression and localization ...
Endokrinologi
Endokrinologi

... receptors, whereas others (eg, steroids, thyroid hormones) bind intracellular receptors that act in the nucleus. Some hormones (eg, estrogens and progestins) bind multiple receptors, which are present on both the cell surface and inside the cell. ...
Review Plasticity of Adult Stem Cells
Review Plasticity of Adult Stem Cells

... derive from a definable and functionally identical pluripotent ICM cell or population of cells that exists within the blastocyst prior to ES cell isolation and culture. In the adult soma, stem cells generally have been thought of as tissue-specific, able to give rise only to progeny cells correspond ...
cell transport notes
cell transport notes

... Ex. A saltwater fish removing salt from its body through the gills. ...
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Cell culture



Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.
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