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A novel live cell assay to measure diacylglycerol lipase α activity
... processes including axonal growth and guidance, adult neurogenesis and retrograde signalling at the synapse. Recent studies have implicated DAGLα as an emerging drug target for several conditions including pain and obesity. Activity assays are critical to the drug discovery process; however, measure ...
... processes including axonal growth and guidance, adult neurogenesis and retrograde signalling at the synapse. Recent studies have implicated DAGLα as an emerging drug target for several conditions including pain and obesity. Activity assays are critical to the drug discovery process; however, measure ...
Cytochrome c Is Released in a Reactive Oxygen
... Rizhsky et al., 2004; van Doorn and Woltering, 2005). However, knowledge of how PCD occurs remains rather obscure, thus necessitating the use of a plant model in which the role of cell components can be investigated in some detail. In this regard, we have shown that, as a result of heat shock (HS) a ...
... Rizhsky et al., 2004; van Doorn and Woltering, 2005). However, knowledge of how PCD occurs remains rather obscure, thus necessitating the use of a plant model in which the role of cell components can be investigated in some detail. In this regard, we have shown that, as a result of heat shock (HS) a ...
Role of Cytoskeleton Changes and Expression
... 1 mg/ml. The stock solution was maintained at -20°C and diluted in culture medium before use. Cell Culture. The origin and maintenance of BJ6 cells and their promotion-sensitive clones have been described (23, 24). Promotable (P+) cells (clone 41, passage 80), nonpromotable (P~) cells (clone 30, pa ...
... 1 mg/ml. The stock solution was maintained at -20°C and diluted in culture medium before use. Cell Culture. The origin and maintenance of BJ6 cells and their promotion-sensitive clones have been described (23, 24). Promotable (P+) cells (clone 41, passage 80), nonpromotable (P~) cells (clone 30, pa ...
Mutant Superoxide Dismutase-1-Linked Familial Amyotrophic
... were plated on poly-L-lysine-coated glass coverslips at a density of 10,000 cells per coverslip in DM EM supplemented with 10% bovine calf serum and 10 mg /ml penicillin /streptomycin (Sigma, St. L ouis, MO). Differentiation of PC12 cells was induced within 24 hr after the addition of DM EM containi ...
... were plated on poly-L-lysine-coated glass coverslips at a density of 10,000 cells per coverslip in DM EM supplemented with 10% bovine calf serum and 10 mg /ml penicillin /streptomycin (Sigma, St. L ouis, MO). Differentiation of PC12 cells was induced within 24 hr after the addition of DM EM containi ...
Skb5, an SH3 adaptor protein, regulates Pmk1 MAPK signaling by
... an adaptor protein and its influence on MAPK activation. However, relatively little is known about the subcellular localization of MAPKKKs and their relevance to MAPK activation. We have been studying the Pmk1 MAPK signaling module, composed of the MAPKKK Mkh1, the MAPKK Pek1 and the MAPK Pmk1, a ke ...
... an adaptor protein and its influence on MAPK activation. However, relatively little is known about the subcellular localization of MAPKKKs and their relevance to MAPK activation. We have been studying the Pmk1 MAPK signaling module, composed of the MAPKKK Mkh1, the MAPKK Pek1 and the MAPK Pmk1, a ke ...
The Ndc80p Complex from Saccharomyces cerevisiae Contains
... Mouse HEC had been identified by us from the EST database as a potential homologue of Ndc80p (Wigge et al., 1998) before the appearance of the human HEC paper (Chen et al., 1997). It is 84% identical to human HEC and both have 643 amino acids. The mouse HEC sequence was derived from mouse ESTs. Imag ...
... Mouse HEC had been identified by us from the EST database as a potential homologue of Ndc80p (Wigge et al., 1998) before the appearance of the human HEC paper (Chen et al., 1997). It is 84% identical to human HEC and both have 643 amino acids. The mouse HEC sequence was derived from mouse ESTs. Imag ...
Pomegranate Ellagitannin–Derived Compounds Exhibit
... however, UB uptake was shown to be greater than that of UA. This result could explain the lower activity of UA in the live cell system. In addition to the differences in UB and UA absorption into the cells, UBS and other isomers have been detected in the media but not the cell lysate in significant ...
... however, UB uptake was shown to be greater than that of UA. This result could explain the lower activity of UA in the live cell system. In addition to the differences in UB and UA absorption into the cells, UBS and other isomers have been detected in the media but not the cell lysate in significant ...
File
... • Sieve elements are unable to sustain independent metabolic activity without the support of a companion cell. • This is because the sieve element cells have no nuclei and fewer organelles (to maximize flow rate). • Plasmodesmata exists between sieve elements and companion cells in ...
... • Sieve elements are unable to sustain independent metabolic activity without the support of a companion cell. • This is because the sieve element cells have no nuclei and fewer organelles (to maximize flow rate). • Plasmodesmata exists between sieve elements and companion cells in ...
Nuclear centering in Spirogyra
... nucleus (with central nucleolus) in Spirogyra crassa. The PNS is associated with helical chloroplast bands in the cell periphery. A Scheme of a longitudinal midplane section, showing postcytokinetic daughter nuclei (right side) positioned eccentrically in the longitudinal direction, and (left side) ...
... nucleus (with central nucleolus) in Spirogyra crassa. The PNS is associated with helical chloroplast bands in the cell periphery. A Scheme of a longitudinal midplane section, showing postcytokinetic daughter nuclei (right side) positioned eccentrically in the longitudinal direction, and (left side) ...
Nuclear Translocation of Fibroblast Growth Factor (FGF) Receptors
... were detected in the nuclear fraction, and < 5 % of the total acid phosphatase activity was associated with the nuclear fraction. For most experiments, we used a rapid method that allows isolation of nuclei from small numbers of cells (Schreiber et al., 1989). Briefly, cells in 35-mm culture dishes ...
... were detected in the nuclear fraction, and < 5 % of the total acid phosphatase activity was associated with the nuclear fraction. For most experiments, we used a rapid method that allows isolation of nuclei from small numbers of cells (Schreiber et al., 1989). Briefly, cells in 35-mm culture dishes ...
Chapter 4 The Cell Membrane, Cytoskeleton, and Cell
... The 2-year-old’s health appeared to be returning mere hours after the liver transplant. Yet, cells of the immune system had already detected the new organ and, interpreting it as “foreign,” began to produce molecules to attack it. Even though the donor’s liver was carefully “matched” to the little g ...
... The 2-year-old’s health appeared to be returning mere hours after the liver transplant. Yet, cells of the immune system had already detected the new organ and, interpreting it as “foreign,” began to produce molecules to attack it. Even though the donor’s liver was carefully “matched” to the little g ...
Posttranscriptional Gene Silencing-2015
... Tumor-derived Exosomes Initiate Tumor Growth in Normal Cells ...
... Tumor-derived Exosomes Initiate Tumor Growth in Normal Cells ...
F-Actin-Dependent Endocytosis of Cell Wall
... strongly suggest that there must be an additional membranous source that feeds into these compartments of plant cells. Our data suggest that this source is the PM. The nature of BFA compartments remains controversial also because several other studies failed to report such compartments, even in plan ...
... strongly suggest that there must be an additional membranous source that feeds into these compartments of plant cells. Our data suggest that this source is the PM. The nature of BFA compartments remains controversial also because several other studies failed to report such compartments, even in plan ...
cell transport and mitosis notes
... Membrane transport—movement of substances into and out of the cell Two methods of transport Passive transport No energy is required, uses kinetic energy ( bouncing particles) Active transport Cell must provide metabolic energy (ATP) ...
... Membrane transport—movement of substances into and out of the cell Two methods of transport Passive transport No energy is required, uses kinetic energy ( bouncing particles) Active transport Cell must provide metabolic energy (ATP) ...
Relative Requirements for Magnesium of Protein and Chlorophyll
... among many possible bases for a Mg requirement. The need for also progressively decreased, with the yield at 20 fLM Mg lowered Mg has been variously ascribed to its occurrence in Chl and its to about 30%o of the control culture at 100 hr. action as a cofactor for a variety of enzymes, notably those ...
... among many possible bases for a Mg requirement. The need for also progressively decreased, with the yield at 20 fLM Mg lowered Mg has been variously ascribed to its occurrence in Chl and its to about 30%o of the control culture at 100 hr. action as a cofactor for a variety of enzymes, notably those ...
Document
... We’re all mixed up kids! How the chromosomes arrange themselves (above or below the equator of the spindle fibres) is a random event. 2 pairs of homologous chromosomes can arrange themselves in 22 ways (giving 4 possible different chromosome combinations in the gametes). 23 pairs of homologous ...
... We’re all mixed up kids! How the chromosomes arrange themselves (above or below the equator of the spindle fibres) is a random event. 2 pairs of homologous chromosomes can arrange themselves in 22 ways (giving 4 possible different chromosome combinations in the gametes). 23 pairs of homologous ...
Isolation and Characterization of Cell Wall
... 40 units per mg rigid layer protein were used] while stirring at 37 "C for 24 h. After centrifugation at 48000g (15 "C, 20 min), the sediment (peptidoglycan-polysaccharidecomplex) was boiled in 4% (w/v) SDS for 15 min and subsequently freed from SDS by washing with distilled water. For hydrofluoric ...
... 40 units per mg rigid layer protein were used] while stirring at 37 "C for 24 h. After centrifugation at 48000g (15 "C, 20 min), the sediment (peptidoglycan-polysaccharidecomplex) was boiled in 4% (w/v) SDS for 15 min and subsequently freed from SDS by washing with distilled water. For hydrofluoric ...
How tissue injury alarms the immune system and causes a systemic
... The long time prevalent theory of the immune system was based on tolerance of self-antigens developed mostly during the fetal life by the deletion of auto-reactive lymphocytes in the thymus and bone marrow. In this theory, only remain during extra utero life immune cells capable of reacting to non–s ...
... The long time prevalent theory of the immune system was based on tolerance of self-antigens developed mostly during the fetal life by the deletion of auto-reactive lymphocytes in the thymus and bone marrow. In this theory, only remain during extra utero life immune cells capable of reacting to non–s ...
Research Area - The University of North Carolina at Chapel Hill
... Rationale: miRNAs play a vital regulatory role in many cell processes and their expression is highly cell typeand differentiation stage-specific. Human bronchial epithelial cells are central participants in the pathogenesis of several extremely important lung diseases and, despite provocative early ...
... Rationale: miRNAs play a vital regulatory role in many cell processes and their expression is highly cell typeand differentiation stage-specific. Human bronchial epithelial cells are central participants in the pathogenesis of several extremely important lung diseases and, despite provocative early ...
The Promise and Challenge of Induced€Pluripotent Stem Cells for
... using retroviral transduction with the same 4 tran- ...
... using retroviral transduction with the same 4 tran- ...
Abstract
... Electron Transfer Flavoprotein (ETF), High Throughput Screen (HTS), Most Probable Number (MPN) ...
... Electron Transfer Flavoprotein (ETF), High Throughput Screen (HTS), Most Probable Number (MPN) ...
A balanced pyrimidine pool is required for optimal Chk1 activation to
... reflect a defect in sister chromatid segregation during anaphase. They cannot be stained with conventional DNA dyes or antibodies against histones, but they can be detected with an antibody against Plk1-interacting checkpoint helicase (PICH, also known as ERCC6L), a protein recruited to UFBs (Bauman ...
... reflect a defect in sister chromatid segregation during anaphase. They cannot be stained with conventional DNA dyes or antibodies against histones, but they can be detected with an antibody against Plk1-interacting checkpoint helicase (PICH, also known as ERCC6L), a protein recruited to UFBs (Bauman ...
Dental pulp - Fresh Men Dentists
... The root canal terminate at the apical foramen, where the pulp and PDL meet and the main nerves and vessels enter and leave the tooth. In the developing tooth the apical foramen is large and centrally located. As the tooth completes its development, the apical foramen become smaller in ...
... The root canal terminate at the apical foramen, where the pulp and PDL meet and the main nerves and vessels enter and leave the tooth. In the developing tooth the apical foramen is large and centrally located. As the tooth completes its development, the apical foramen become smaller in ...
cnidarian key
... polyp, diploid medusa therefore mitosis involved, therefore asexual) 7. What are the structures that aid mobility of the planula called? cilia 8. Describe how a medusa moves through the water. Ring of “muscle” around edge of medusa contracts and relaxes, forcing water out of bell-shaped medusa and p ...
... polyp, diploid medusa therefore mitosis involved, therefore asexual) 7. What are the structures that aid mobility of the planula called? cilia 8. Describe how a medusa moves through the water. Ring of “muscle” around edge of medusa contracts and relaxes, forcing water out of bell-shaped medusa and p ...
Preview for 2/6/02 – Dr
... Chapter 26: Bacteria and Archaea: the Prokaryotic Domains Cell division Asexual reproduction by cell division via binary fission Mechanisms of gene transfer -transformation -conjugation -transduction Adaptation short generation time allows favorable mutations and novel genomes arising from gene tra ...
... Chapter 26: Bacteria and Archaea: the Prokaryotic Domains Cell division Asexual reproduction by cell division via binary fission Mechanisms of gene transfer -transformation -conjugation -transduction Adaptation short generation time allows favorable mutations and novel genomes arising from gene tra ...
Cell culture
![](https://commons.wikimedia.org/wiki/Special:FilePath/Cell_Culture_in_a_tiny_Petri_dish.jpg?width=300)
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.