Enriched Motor Neuron Populations Derived From Bacterial Artificial
... by quantitative reverse transcriptase polymerase chain reaction for the Hb9, Isl1, Lim3 and ChAT (choline acetyltransferase, the enzyme responsible for acetylcholine biosynthesis in motor neurons) transcripts. We found robust enrichment (31.9 ⫾ 19.5-fold, n ⫽ 3) for Hb9 in the GFP-positive pool comp ...
... by quantitative reverse transcriptase polymerase chain reaction for the Hb9, Isl1, Lim3 and ChAT (choline acetyltransferase, the enzyme responsible for acetylcholine biosynthesis in motor neurons) transcripts. We found robust enrichment (31.9 ⫾ 19.5-fold, n ⫽ 3) for Hb9 in the GFP-positive pool comp ...
Supplement
... apical localization of N-cadherin in GFP epithelial somatopleure cells (yellow arrowheads and white arrows respectively) and relocalization of N-cadherin and increased Vimentin staining in GFP positive cells that have left the somatopleure epithelium (G to H, white arrowheads).(L to O) Transverse se ...
... apical localization of N-cadherin in GFP epithelial somatopleure cells (yellow arrowheads and white arrows respectively) and relocalization of N-cadherin and increased Vimentin staining in GFP positive cells that have left the somatopleure epithelium (G to H, white arrowheads).(L to O) Transverse se ...
CELL STRUCTURE AND FUNCTION CHAPTER3
... The cell marks the boundary between the nonliving and the living. The molecules that serve as food for a cell and the macromolecules that make up a cell are not alive, and yet the cell is alive. Thus, the answer to what life is must lie within the cell, because the smallest living organisms are unic ...
... The cell marks the boundary between the nonliving and the living. The molecules that serve as food for a cell and the macromolecules that make up a cell are not alive, and yet the cell is alive. Thus, the answer to what life is must lie within the cell, because the smallest living organisms are unic ...
Where stem cells call home
... mixes chosen soluble stem cell niche components, such as growth factors or ECM proteins, in nanoliter volumes. The mixtures are then dispensed in high throughput onto a substrate where they generate spots with a wide range of defined properties. These spots are niches that can then be tested for th ...
... mixes chosen soluble stem cell niche components, such as growth factors or ECM proteins, in nanoliter volumes. The mixtures are then dispensed in high throughput onto a substrate where they generate spots with a wide range of defined properties. These spots are niches that can then be tested for th ...
The 2016 Nobel Prize in Physiology or Medicine
... The Nobel Prize in Physiology or Medicine for 2016 was awarded to Yoshinori Ohsumi (Japan) for his work on autophagy. Autophagy, meaning ‘self-eating’, is the process where the cell content like large macromolecule complexes or organelles are fused with the lysosome for degradation and recycling. Wh ...
... The Nobel Prize in Physiology or Medicine for 2016 was awarded to Yoshinori Ohsumi (Japan) for his work on autophagy. Autophagy, meaning ‘self-eating’, is the process where the cell content like large macromolecule complexes or organelles are fused with the lysosome for degradation and recycling. Wh ...
A Mechanism for Asymmetric Cell Division Resulting in Proliferative
... 2/ cells completely restored formation of both asymmetrically dividing and G0-like cells, while overexpression of AKT2 did not, suggesting that AKT1 is both necessary and sufficient for the production of G0-like cells (Fig. 1B). On the basis of this result, we used site-directed mutagenesis to iden ...
... 2/ cells completely restored formation of both asymmetrically dividing and G0-like cells, while overexpression of AKT2 did not, suggesting that AKT1 is both necessary and sufficient for the production of G0-like cells (Fig. 1B). On the basis of this result, we used site-directed mutagenesis to iden ...
Chapter 19: Gene Targeting And Transgenic Technologies
... surgically transferred into the oviduct of pseudopregnant mice. Pseudopregnant females are generated by matings with vasectomized males. The act of copulation initiates the endocrine changes of pregnancy, providing a suitable uterine environment for the survival and implantation of the transferred e ...
... surgically transferred into the oviduct of pseudopregnant mice. Pseudopregnant females are generated by matings with vasectomized males. The act of copulation initiates the endocrine changes of pregnancy, providing a suitable uterine environment for the survival and implantation of the transferred e ...
Is cytoskeletal tension a major determinant of cell - AJP-Cell
... stiffness by another 10% (data not shown). To confirm that the CSK actin lattice contributed to the observed stretch-induced stiffening, adherent cells were stretched before and after addition of Cyto D, which disrupts the actin lattice. Addition of Cyto D (1 µg/ml for 30 min) resulted in a 40% redu ...
... stiffness by another 10% (data not shown). To confirm that the CSK actin lattice contributed to the observed stretch-induced stiffening, adherent cells were stretched before and after addition of Cyto D, which disrupts the actin lattice. Addition of Cyto D (1 µg/ml for 30 min) resulted in a 40% redu ...
L-form bacteria, cell walls and the origins of life
... have generated outcomes remarkably reminiscent of the proliferation of L-form cells. One of the most important theoretical requirements for the evolution of life is a mechanism for encapsulating nucleic acids and the products of replication and gene expression [23]. A related requirement is for the ...
... have generated outcomes remarkably reminiscent of the proliferation of L-form cells. One of the most important theoretical requirements for the evolution of life is a mechanism for encapsulating nucleic acids and the products of replication and gene expression [23]. A related requirement is for the ...
supplement
... our model. We use a simple picture in which f stays constant throughout a cell cycle and changes stochastically at mitosis, chosen for simplicity and due to the observed slowly varying behaviour of membrane potential in the cell cycle [1]. An alternative picture involves f being allowed to vary cont ...
... our model. We use a simple picture in which f stays constant throughout a cell cycle and changes stochastically at mitosis, chosen for simplicity and due to the observed slowly varying behaviour of membrane potential in the cell cycle [1]. An alternative picture involves f being allowed to vary cont ...
Cells
... 7.3 The Plasma Membrane Proteins Transmit signals inside the cell Act as a support structure Provide pathways for larger substances to enter and leave the cell ...
... 7.3 The Plasma Membrane Proteins Transmit signals inside the cell Act as a support structure Provide pathways for larger substances to enter and leave the cell ...
The bacterial cell envelope - Philosophical Transactions of the
... the rise in multidrug resistance. All the complex functions carried out by bacteria require a high degree of organization, and much of the recent excitement regarding envelope biology stems from our newly formed appreciation of this organization. The reviews published in this collection reflect some ...
... the rise in multidrug resistance. All the complex functions carried out by bacteria require a high degree of organization, and much of the recent excitement regarding envelope biology stems from our newly formed appreciation of this organization. The reviews published in this collection reflect some ...
RCSB Molecule of the Month - Neurotransmitter Transporters
... Neurotransmitter transporters have been difficult to study by x-ray crystallography, but structures have been obtained for several bacterial transporters with very similar function. These transporters import neurotransmitter-like nutrient molecules into the bacterial cell, powered by the co-transpor ...
... Neurotransmitter transporters have been difficult to study by x-ray crystallography, but structures have been obtained for several bacterial transporters with very similar function. These transporters import neurotransmitter-like nutrient molecules into the bacterial cell, powered by the co-transpor ...
Transport Within Cells
... Interesting Scientific Fact: Osmosis, diffusion, facilitated diffusion do not require any energy from the cell. This is why these are forms of PASSIVE TRANSPORT. However, if they were to stop, the cell would die. Question 11. What helps molecules across the membrane during facilitated diffusion. Why ...
... Interesting Scientific Fact: Osmosis, diffusion, facilitated diffusion do not require any energy from the cell. This is why these are forms of PASSIVE TRANSPORT. However, if they were to stop, the cell would die. Question 11. What helps molecules across the membrane during facilitated diffusion. Why ...
Homeostasis, Transport, and Bioenergetics
... selectively permeable membrane in which water moves from a solution containing a low concentration of solute to a solution containing a high concentration of solute. Solutes are substances, like salt, sugar, or food coloring, that are dissolved into a solvent, like water. Osmosis plays a role in cel ...
... selectively permeable membrane in which water moves from a solution containing a low concentration of solute to a solution containing a high concentration of solute. Solutes are substances, like salt, sugar, or food coloring, that are dissolved into a solvent, like water. Osmosis plays a role in cel ...
The cell biology of neural stem and progenitor cells - MPI
... have been linked to ciliary function: the sonic hedgehog (shh) and the wnt pathways [33,34]. Thus, shh signaling via the primary cilium has a crucial role for the expansion of neural progenitors during adult neurogenesis in the dentate gyrus of the hippocampus [35] and during neurogenesis in the dev ...
... have been linked to ciliary function: the sonic hedgehog (shh) and the wnt pathways [33,34]. Thus, shh signaling via the primary cilium has a crucial role for the expansion of neural progenitors during adult neurogenesis in the dentate gyrus of the hippocampus [35] and during neurogenesis in the dev ...
The Plant Secretory Pathway: An Essential
... functions as a secretory vesicle or as an endosome. Because of the difficulty in detecting polysaccharides using a method similar to that used for vesicular proteomic analysis, secretion of polysaccharides has been investigated mostly using immunoelectron microscopy with antibodies recognizing carbo ...
... functions as a secretory vesicle or as an endosome. Because of the difficulty in detecting polysaccharides using a method similar to that used for vesicular proteomic analysis, secretion of polysaccharides has been investigated mostly using immunoelectron microscopy with antibodies recognizing carbo ...
REVIEW ARTICLE Genome Organization and Expression of
... small subunits do not completely disengage at the end of a given ORF and protein synthesis is reinitiated internally in the RNA (Sieg & Gronenborn, 1982). Because ORFs I to III tandemly overlap one another slightly (see Fig. 1), this makes possible a frameshifting mechanism similar to that demonstra ...
... small subunits do not completely disengage at the end of a given ORF and protein synthesis is reinitiated internally in the RNA (Sieg & Gronenborn, 1982). Because ORFs I to III tandemly overlap one another slightly (see Fig. 1), this makes possible a frameshifting mechanism similar to that demonstra ...
Slide 1 (Early Prophase): What is interphase often
... In cell D each chromosome has doubled and the two parts are separating. As the split rods move away from each other, they shape themselves into what may be described as two V’s facing each other. Spindle fibers are faint but visible at S in the lower part of the cell. Their function is to pull the n ...
... In cell D each chromosome has doubled and the two parts are separating. As the split rods move away from each other, they shape themselves into what may be described as two V’s facing each other. Spindle fibers are faint but visible at S in the lower part of the cell. Their function is to pull the n ...
O TICS - Phi Optics
... mean cell mass evolves synchronously in time with the total mass of the entire population during the duration of a (mean) cell cycle, i.e., 22–26 h, after which it levels off. This finding indicates that after one cell cycle, the culture loses synchrony and the single cell mass is limited by mitosis ...
... mean cell mass evolves synchronously in time with the total mass of the entire population during the duration of a (mean) cell cycle, i.e., 22–26 h, after which it levels off. This finding indicates that after one cell cycle, the culture loses synchrony and the single cell mass is limited by mitosis ...
10.2 Process of Cell Division
... separate to become individual chromosomes. The chromosomes separate into two groups near the poles of the ...
... separate to become individual chromosomes. The chromosomes separate into two groups near the poles of the ...
B Cell Tolerance in Health and Disease
... either eliminated or suppressed on recognition of their specific self-antigen. These studies indicated that when an immature B cell reacts with a self-antigen with high avidity, such as a highly expressed membrane-bound protein, it undergoes apoptosis within 2–3 d [3,18]. In contrast, low avidity ...
... either eliminated or suppressed on recognition of their specific self-antigen. These studies indicated that when an immature B cell reacts with a self-antigen with high avidity, such as a highly expressed membrane-bound protein, it undergoes apoptosis within 2–3 d [3,18]. In contrast, low avidity ...
Modulation of the Cell Cycle-dependent
... in various cell cycle phases without prior growth-perturbing manipulation. Treatment of these subpopulations with novobiocin (NOVO), a putative inhibitor of the mammalian topoisomerase II enzyme, revealed a unique cell cycle phase-dependent cytotoxicity for this agent. At a concentration of 0.3 m\i, ...
... in various cell cycle phases without prior growth-perturbing manipulation. Treatment of these subpopulations with novobiocin (NOVO), a putative inhibitor of the mammalian topoisomerase II enzyme, revealed a unique cell cycle phase-dependent cytotoxicity for this agent. At a concentration of 0.3 m\i, ...
Biology 11 - hrsbstaff.ednet.ns.ca
... Explain how unicellular organisms differ from multicellular organisms with respect to how they perform their life processes. Describe how cells are arranged into increasingly complex levels of cellular organization in multicellular organisms - tissues; organs; organ systems Explain why it is necessa ...
... Explain how unicellular organisms differ from multicellular organisms with respect to how they perform their life processes. Describe how cells are arranged into increasingly complex levels of cellular organization in multicellular organisms - tissues; organs; organ systems Explain why it is necessa ...
Herceptin Resistance Database for Understanding
... lines. To have a general and gross overview we have provided search and browse options respectively. The significant feature, in both search and browse options, is the display of genomic feature (mutation and expression) of certain important genes, which are the characteristic of a particular cell l ...
... lines. To have a general and gross overview we have provided search and browse options respectively. The significant feature, in both search and browse options, is the display of genomic feature (mutation and expression) of certain important genes, which are the characteristic of a particular cell l ...