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... addition, ribosomes line the walls of the internal membrane of the ‘‘nuclear compartment’’ [8], as observed along the inner membrane (IM) of classical bacteria. This and other analyses have led to the suggestion that the PVC cell plan is different from ‘‘classical’’ Gram-negative bacteria, such as E ...
... addition, ribosomes line the walls of the internal membrane of the ‘‘nuclear compartment’’ [8], as observed along the inner membrane (IM) of classical bacteria. This and other analyses have led to the suggestion that the PVC cell plan is different from ‘‘classical’’ Gram-negative bacteria, such as E ...
Supplementary Notes - Word file
... expressing cells, 77 percent of Rec8 obtained from Cdc20-depleted cells, and 65 percent of Rec8 obtained from Cdc5-depleted cells (Supplemental Fig. 2a-c). The overall coverage of Rec8 from arrests in which Rec8 is phosphorylated was 85 percent. Selected regions (Supplemental Fig. 2a-c) were not ame ...
... expressing cells, 77 percent of Rec8 obtained from Cdc20-depleted cells, and 65 percent of Rec8 obtained from Cdc5-depleted cells (Supplemental Fig. 2a-c). The overall coverage of Rec8 from arrests in which Rec8 is phosphorylated was 85 percent. Selected regions (Supplemental Fig. 2a-c) were not ame ...
Biological Industries Israel Beit Haemek Ltd. Product
... Blood cell karyotyping is an important tool in modern Human Cytogenetics. Karyotyping is just one of the numerous techniques that allow us to investigate several thousands of possible genetic developmental abnormalities, chromosomal defects, their frequency in the population at-large and the relatio ...
... Blood cell karyotyping is an important tool in modern Human Cytogenetics. Karyotyping is just one of the numerous techniques that allow us to investigate several thousands of possible genetic developmental abnormalities, chromosomal defects, their frequency in the population at-large and the relatio ...
Negative regulation of the interferon response by
... transcribed into long non-coding transcripts (1,2). These transcripts, the long non-coding RNAs (lncRNAs), constitute a novel layer of regulatory factors with important roles in almost every aspect of cellular function (3–9). The expression of many lncRNAs is tightly regulated by various cellular si ...
... transcribed into long non-coding transcripts (1,2). These transcripts, the long non-coding RNAs (lncRNAs), constitute a novel layer of regulatory factors with important roles in almost every aspect of cellular function (3–9). The expression of many lncRNAs is tightly regulated by various cellular si ...
CRISPR/Cas9 Screens Reveal Requirements for Host Cell Sulfation
... sgRNAs targeting multiple genes that contribute to a single biological process. Based on a statistically significant enrichment (p < 0.001) in both biological replicates, T3SS1 resistance was associated with sgRNAs targeting multiple genes important for cell surface sulfation, the SWI/SNF chromatin ...
... sgRNAs targeting multiple genes that contribute to a single biological process. Based on a statistically significant enrichment (p < 0.001) in both biological replicates, T3SS1 resistance was associated with sgRNAs targeting multiple genes important for cell surface sulfation, the SWI/SNF chromatin ...
Molecular Biology - Gateway Engineering Education Coalition
... quantities of peptides and proteins, some of which are as small as a dozen amino acids on up to proteins containing hundreds of amino acids. The proteins we want to make (perhaps using bacteria to produce them) might be human hormones, antibodies and other rare proteins that are difficult or impossi ...
... quantities of peptides and proteins, some of which are as small as a dozen amino acids on up to proteins containing hundreds of amino acids. The proteins we want to make (perhaps using bacteria to produce them) might be human hormones, antibodies and other rare proteins that are difficult or impossi ...
The endoplasmic reticulum exerts control over organelle streaming
... that rhd3 loss resulted in a reduction of ER streaming velocity compared with that of the wild type at 12 DAG (Fig. 2C), when the differences in ER streaming velocities between wild type and the rhd3 mutant are markedly different (Fig. 1B). The maximal streaming velocity of the ER in fully expanded ...
... that rhd3 loss resulted in a reduction of ER streaming velocity compared with that of the wild type at 12 DAG (Fig. 2C), when the differences in ER streaming velocities between wild type and the rhd3 mutant are markedly different (Fig. 1B). The maximal streaming velocity of the ER in fully expanded ...
Reflection Paper on stem cell-based medicinal - EMA
... Stem cells hold considerable promise as a source of cells for therapeutic applications in various conditions, including metabolic, degenerative and inflammatory diseases, for the repair and regeneration of damaged or lost tissues and also in the treatment of cancer. Various stem cell types can be is ...
... Stem cells hold considerable promise as a source of cells for therapeutic applications in various conditions, including metabolic, degenerative and inflammatory diseases, for the repair and regeneration of damaged or lost tissues and also in the treatment of cancer. Various stem cell types can be is ...
Human Monocytes Arachidonic Acid Reacylation Reactions in
... the AA has been incorporated into PLs, a remodeling process carried out by CoA-independent transacylase transfers AA from choline glycerophospholipids (PCs) to ethanolamine glycerophospholipids, in a process that takes several hours to take place in primary cells but is strikingly rapid in tumor cel ...
... the AA has been incorporated into PLs, a remodeling process carried out by CoA-independent transacylase transfers AA from choline glycerophospholipids (PCs) to ethanolamine glycerophospholipids, in a process that takes several hours to take place in primary cells but is strikingly rapid in tumor cel ...
Yeast as a screening tool
... cleavage of the amyloid precursor protein (APP) by the bsecretase (BACE) and the g-secretase. Because BACE knockout mice have significantly reduced Ab levels in the brain, but develop normally and show no apparent phenotype, BACE is considered an ideal therapeutic target for treating AD. We have est ...
... cleavage of the amyloid precursor protein (APP) by the bsecretase (BACE) and the g-secretase. Because BACE knockout mice have significantly reduced Ab levels in the brain, but develop normally and show no apparent phenotype, BACE is considered an ideal therapeutic target for treating AD. We have est ...
Tet Proteins Can Convert 5-Methylcytosine to 5
... used as a substrate (Fig. 3B, left) strongly suggests that Tet-catalyzed iterative oxidation is likely a kinetically relevant pathway. To determine whether Tet-catalyzed iterative oxidation of 5mC can take place in vivo, we transfected a mammalian expression construct containing the Tet2 catalytic d ...
... used as a substrate (Fig. 3B, left) strongly suggests that Tet-catalyzed iterative oxidation is likely a kinetically relevant pathway. To determine whether Tet-catalyzed iterative oxidation of 5mC can take place in vivo, we transfected a mammalian expression construct containing the Tet2 catalytic d ...
Chemotaxis
... attractant; highest when not adapted • model can account for observed dynamic range Is there any data to support this clustering? Maddock and Shapiro (1993) • immunofluorescence and immuno-EM studies of receptors, CheA, CheW ...
... attractant; highest when not adapted • model can account for observed dynamic range Is there any data to support this clustering? Maddock and Shapiro (1993) • immunofluorescence and immuno-EM studies of receptors, CheA, CheW ...
BALB/c 3T3 cell transformation assays for the assessment
... are spindle-shaped, multilayered, dense and with basophilic staining, and show random orientation at the focus edge. The most transformed BALB/c 3T3 cells are tumorigenic in nude mice. At present, the clone A31-1-1 is used for the BALB/c 3T3 cell transformation assays, unless otherwise specified. Th ...
... are spindle-shaped, multilayered, dense and with basophilic staining, and show random orientation at the focus edge. The most transformed BALB/c 3T3 cells are tumorigenic in nude mice. At present, the clone A31-1-1 is used for the BALB/c 3T3 cell transformation assays, unless otherwise specified. Th ...
Labeled Schwann cell transplantation
... injury, but that some cells survive the first week and integrate into the spinal cord only to be lost in a second wave of cell death occurring between 8 and 14 days. To prove that disappearance of PLAP protein expression was not due to transgene downregulation, PCR was performed to detect PLAP DNA at ...
... injury, but that some cells survive the first week and integrate into the spinal cord only to be lost in a second wave of cell death occurring between 8 and 14 days. To prove that disappearance of PLAP protein expression was not due to transgene downregulation, PCR was performed to detect PLAP DNA at ...
Rearrangement of the Keratin Cytoskeleton after Combined
... cytochalasin D in inducing keratin rearrangement. Both cytochalasin B and cytochalasin D were effective in combination with demecolcine or vinblastine sulfate (Table 1). Cytochalasin D in combination with fl-lumicolchicine had a markedly reduced effect on the organization of cytokeratins (Table 1) . ...
... cytochalasin D in inducing keratin rearrangement. Both cytochalasin B and cytochalasin D were effective in combination with demecolcine or vinblastine sulfate (Table 1). Cytochalasin D in combination with fl-lumicolchicine had a markedly reduced effect on the organization of cytokeratins (Table 1) . ...
Degradation of DNA damage-independently stalled RNA
... Figure 2. Rpb1 is polyubiquitylated upon impairment of transcription elongation. (A) Polyubiquitylation of Rpb1 is increased in Δctk1, Δdst1, Δtho2 and Δbur2 cells. Approximately equal amounts of RNAPII were purified using Rpb3-TAP and limiting amounts of beads in the presence of DUB and proteasome ...
... Figure 2. Rpb1 is polyubiquitylated upon impairment of transcription elongation. (A) Polyubiquitylation of Rpb1 is increased in Δctk1, Δdst1, Δtho2 and Δbur2 cells. Approximately equal amounts of RNAPII were purified using Rpb3-TAP and limiting amounts of beads in the presence of DUB and proteasome ...
Research Article Immunohistochemical and
... articular cartilage defects. Therefore, periosteal autografts have been used as flaps with the cambium cell layer facing towards the defect the articular cartilage [8]. In addition, periosteum has also been described as being able to induce heterotopic bone formation when implanted into muscle as a ...
... articular cartilage defects. Therefore, periosteal autografts have been used as flaps with the cambium cell layer facing towards the defect the articular cartilage [8]. In addition, periosteum has also been described as being able to induce heterotopic bone formation when implanted into muscle as a ...
Rearrangement of the Keratin Cytoskeleton after
... Drug-induced changes in cytokeratin organization in HeLa or FME cells were tested with various microtubule- or microfilament-inhibiting compounds. A dose response for each microtubule or microfilament inhibitor was tested either alone or in binary combination over a 2-h period . Colchicine, Colcemid ...
... Drug-induced changes in cytokeratin organization in HeLa or FME cells were tested with various microtubule- or microfilament-inhibiting compounds. A dose response for each microtubule or microfilament inhibitor was tested either alone or in binary combination over a 2-h period . Colchicine, Colcemid ...
Get PDF file - Botanik in Bonn
... mitosomes, have sizes below 500 nm and lack any DNA.28 Nevertheless, these mini-cells can reproduce themselves as their numbers are maintained at about 250 for one host cell.29 Both mitosomes and hydrogenosomes provide us with convincing evidences that DNA can be completely removed from one partner ...
... mitosomes, have sizes below 500 nm and lack any DNA.28 Nevertheless, these mini-cells can reproduce themselves as their numbers are maintained at about 250 for one host cell.29 Both mitosomes and hydrogenosomes provide us with convincing evidences that DNA can be completely removed from one partner ...
Exosome-Depleted FBS Using Beckman Coulter Centrifugation:
... Beckman Coulter Life Sciences, Indianapolis, IN 46268 Exosomes are small microvesicles, derived from the late endosome, most often described in the literature to be less than 120 nm, released by all cell types, and proven to be involved in cancer metastasis1-3. Exosome characterization and analysis ...
... Beckman Coulter Life Sciences, Indianapolis, IN 46268 Exosomes are small microvesicles, derived from the late endosome, most often described in the literature to be less than 120 nm, released by all cell types, and proven to be involved in cancer metastasis1-3. Exosome characterization and analysis ...
Stem cells in the light of evolution
... stem cell research and early studies in ancient organisms. It also outlines details on stem cells in the light of evolution with an emphasis on their regeneration potential, coloniality and multicellularity. The information provided might be of use to molecular biologists, medical scientists and dev ...
... stem cell research and early studies in ancient organisms. It also outlines details on stem cells in the light of evolution with an emphasis on their regeneration potential, coloniality and multicellularity. The information provided might be of use to molecular biologists, medical scientists and dev ...
LvDelta induces mesoderm and endoderm
... previously (Zhu et al., 2001). The same probe used in the northern analysis of LvDelta was used for in situ hybridization. mRNA injection The full-length LvDelta clone was linearized with Xho1 and used as a template to generate 5′ capped mRNA using the T3 mMessage mMachine kit (Ambion). In addition, ...
... previously (Zhu et al., 2001). The same probe used in the northern analysis of LvDelta was used for in situ hybridization. mRNA injection The full-length LvDelta clone was linearized with Xho1 and used as a template to generate 5′ capped mRNA using the T3 mMessage mMachine kit (Ambion). In addition, ...
Membranes - Active Transport (GPC)
... carries one speci c ion or molecule. A symporter carries two dierent ions or molecules, both in the same direction. An antiporter also carries two dierent ions or molecules, but in dierent directions. All of these transporters can also transport small, uncharged organic molecules like glucose. Th ...
... carries one speci c ion or molecule. A symporter carries two dierent ions or molecules, both in the same direction. An antiporter also carries two dierent ions or molecules, but in dierent directions. All of these transporters can also transport small, uncharged organic molecules like glucose. Th ...