Xian`s Southern Blot Protocol Using Digoxigenin Labeled Probe
... • run gel at appropriate voltage to obtain clean bands; stain and photograph with size reference • smaller fragments transfer more efficiently. for very large DNAs, a 2min UV nicking step on a short wave transilluminator can be added • transfer gel to a sealable Tupperware container traditional meth ...
... • run gel at appropriate voltage to obtain clean bands; stain and photograph with size reference • smaller fragments transfer more efficiently. for very large DNAs, a 2min UV nicking step on a short wave transilluminator can be added • transfer gel to a sealable Tupperware container traditional meth ...
THE SMOOTH MUSCLE CELL II. Growth of Smooth Muscle in
... buffer (pH 7.3) (4) was added to the cells in situ. They were fixed at room temperature for 20 min. The cells were then washed with fresh culture medium for 15 rain and postfixed for 30 rain at room temperature with a 2 % osmium tetroxide solution buffered with s-collidine at pH 7.3. They were dehyd ...
... buffer (pH 7.3) (4) was added to the cells in situ. They were fixed at room temperature for 20 min. The cells were then washed with fresh culture medium for 15 rain and postfixed for 30 rain at room temperature with a 2 % osmium tetroxide solution buffered with s-collidine at pH 7.3. They were dehyd ...
Thylakoid biogenesis has joined the new era of bacterial cell biology
... 2009; Bohne et al., 2013). Locations of translation were defined as sites where mRNAs and these proteins of the chloroplast translation machinery co-localized when the PSII core subunit synthesis was induced for de novo assembly of the complex by light. Based on these criteria, a specific cytologica ...
... 2009; Bohne et al., 2013). Locations of translation were defined as sites where mRNAs and these proteins of the chloroplast translation machinery co-localized when the PSII core subunit synthesis was induced for de novo assembly of the complex by light. Based on these criteria, a specific cytologica ...
Xian`s Southern Blot Protocol Using Digoxigenin Labeled Probe
... • denature DNA by boiling 10min; quickly chill on ice to prevent reannealing of strands • add 4µl DIG high prime labeling mix; mix briefly and tap spin • incubate overnight at 37°C. • stop reaction by adding 2µl 0.2M EDTA (pH 8) and heat inactivate at 65°C 10min • boil probe 10-20min before using • ...
... • denature DNA by boiling 10min; quickly chill on ice to prevent reannealing of strands • add 4µl DIG high prime labeling mix; mix briefly and tap spin • incubate overnight at 37°C. • stop reaction by adding 2µl 0.2M EDTA (pH 8) and heat inactivate at 65°C 10min • boil probe 10-20min before using • ...
A tour of the cell - The Open University
... The subcellular components can then be recovered by centrifugation. The cell homogenate is dispensed into centrifuge tubes which are placed into a rotating holder (known as a rotor) that fits into the centrifuge. As the rotor turns, particles suspended in the homogenate migrate towards the bottom of ...
... The subcellular components can then be recovered by centrifugation. The cell homogenate is dispensed into centrifuge tubes which are placed into a rotating holder (known as a rotor) that fits into the centrifuge. As the rotor turns, particles suspended in the homogenate migrate towards the bottom of ...
Arabidopsis R-SNARE Proteins VAMP721 and VAMP722 Are
... in dividing cells and its ability to interact with KN; however, the T-DNA insertion lines of the NPSN11 gene developed normally as the wild-type plants [18]. Therefore, evidence for the function of R-SNARE proteins in plant cytokinesis is still insufficient. In this study, we investigated the functi ...
... in dividing cells and its ability to interact with KN; however, the T-DNA insertion lines of the NPSN11 gene developed normally as the wild-type plants [18]. Therefore, evidence for the function of R-SNARE proteins in plant cytokinesis is still insufficient. In this study, we investigated the functi ...
classkingdomsppt
... It is not always an easy thing to tell the difference between living, dead, and non-living things. Prior to the 1600's many people believed in Spontaneous Generation. ...
... It is not always an easy thing to tell the difference between living, dead, and non-living things. Prior to the 1600's many people believed in Spontaneous Generation. ...
Sterilization, Disinfection and Antibacterial Agents
... Desiccation: In the absence of water, microbes cannot grow or reproduce, but some may remain viable for years. After water becomes available, they start growing again. Susceptibility to desiccation varies widely: Neisseria gonnorrhea: Only survives about one hour. Mycobacterium tuberculosis: May ...
... Desiccation: In the absence of water, microbes cannot grow or reproduce, but some may remain viable for years. After water becomes available, they start growing again. Susceptibility to desiccation varies widely: Neisseria gonnorrhea: Only survives about one hour. Mycobacterium tuberculosis: May ...
The connection of cytoskeletal network with plasma membrane and
... spatially and temporarily regulating the transportation and deposition of cell wall components. This tight control is achieved by the dynamic behavior of the cytoskeletons, but also through the tethering of these structures to the plasma membrane. This tethering may also extend beyond the plasma mem ...
... spatially and temporarily regulating the transportation and deposition of cell wall components. This tight control is achieved by the dynamic behavior of the cytoskeletons, but also through the tethering of these structures to the plasma membrane. This tethering may also extend beyond the plasma mem ...
bacterial growth and measurement-2012
... i) Phase of Decreased Growth: rate of fission begins to decline and the organisms die in decreasing numbers so that the increase in the number of cells slows down. This is due to a number of factors such as depletion of nutrients and accumulation of toxic waste, pH changes, hydrogen acceptors are us ...
... i) Phase of Decreased Growth: rate of fission begins to decline and the organisms die in decreasing numbers so that the increase in the number of cells slows down. This is due to a number of factors such as depletion of nutrients and accumulation of toxic waste, pH changes, hydrogen acceptors are us ...
Chapter 5: Attachment and entry of viruses into cells 1. Overview of
... 4. Infection = entry into the cell of Virus genome (+ few associated proteins) / capsid, any associated appendages remain at the cell surface >< animal and plant viruses (entire virion or at least nucleocapsid enter host cell) 5. Delivery of phage genome require penetration of cell wall / slim layer ...
... 4. Infection = entry into the cell of Virus genome (+ few associated proteins) / capsid, any associated appendages remain at the cell surface >< animal and plant viruses (entire virion or at least nucleocapsid enter host cell) 5. Delivery of phage genome require penetration of cell wall / slim layer ...
Slide - Smith Lab
... • After a process of maturation including lipid synthesis and accumulation, centripetal cell movement, and eventual cell degeneration and membrane disintegration, the lipids and other cell components are shed into the lumen of the ductal system. • This holocrine secretion process hence results in th ...
... • After a process of maturation including lipid synthesis and accumulation, centripetal cell movement, and eventual cell degeneration and membrane disintegration, the lipids and other cell components are shed into the lumen of the ductal system. • This holocrine secretion process hence results in th ...
Tonicity, which is directly related to the osmolarity of a
... solute particles; a solution with high osmolarity has fewer water molecules with respect to solute particles. In a situation in which solutions of two different osmolarities are separated by a membranepermeable to water, though not to the solute, water will move from the side of the membrane with lo ...
... solute particles; a solution with high osmolarity has fewer water molecules with respect to solute particles. In a situation in which solutions of two different osmolarities are separated by a membranepermeable to water, though not to the solute, water will move from the side of the membrane with lo ...
File - John Robert Warner
... _____ 11. Which of the following is NOT a step in the light-dependent reactions? a. High-energy electrons move through the electron transport chain. b. Pigments in photosystem II absorb light. c. ATP synthase allows H+ ions to pass through the thylakoid membrane. d. ATP and NADPH are used to produce ...
... _____ 11. Which of the following is NOT a step in the light-dependent reactions? a. High-energy electrons move through the electron transport chain. b. Pigments in photosystem II absorb light. c. ATP synthase allows H+ ions to pass through the thylakoid membrane. d. ATP and NADPH are used to produce ...
Name
... _____ 11. Which of the following is NOT a step in the light-dependent reactions? a. High-energy electrons move through the electron transport chain. b. Pigments in photosystem II absorb light. c. ATP synthase allows H+ ions to pass through the thylakoid membrane. d. ATP and NADPH are used to produce ...
... _____ 11. Which of the following is NOT a step in the light-dependent reactions? a. High-energy electrons move through the electron transport chain. b. Pigments in photosystem II absorb light. c. ATP synthase allows H+ ions to pass through the thylakoid membrane. d. ATP and NADPH are used to produce ...
HIV-1 Infection of Nondividing Cells: C-Terminal
... Taken together, these results demonstrate that myristoylation acts as a dominant targeting signal for MA during viral particle formation, yet for 1% of the molecules, it becomes recessive once HIV-1 enters target cells. This suggests a model in which some modification of MA, during or after assembly ...
... Taken together, these results demonstrate that myristoylation acts as a dominant targeting signal for MA during viral particle formation, yet for 1% of the molecules, it becomes recessive once HIV-1 enters target cells. This suggests a model in which some modification of MA, during or after assembly ...
Photosynthesis - John A. Ferguson Senior High School
... _____ 11. Which of the following is NOT a step in the light-dependent reactions? a. High-energy electrons move through the electron transport chain. b. Pigments in photosystem II absorb light. c. ATP synthase allows H+ ions to pass through the thylakoid membrane. d. ATP and NADPH are used to produce ...
... _____ 11. Which of the following is NOT a step in the light-dependent reactions? a. High-energy electrons move through the electron transport chain. b. Pigments in photosystem II absorb light. c. ATP synthase allows H+ ions to pass through the thylakoid membrane. d. ATP and NADPH are used to produce ...
100 - A Primer on Calf Nutition
... carbohydrates include sugars (glucose, sucrose, lactose), starch, glycogen, and cellulose. Many different carbohydrates that are consumed by animals are ultimately converted to glucose, which is an essential nutrient required by many tissues in the body. For more information about carbohydrates and ...
... carbohydrates include sugars (glucose, sucrose, lactose), starch, glycogen, and cellulose. Many different carbohydrates that are consumed by animals are ultimately converted to glucose, which is an essential nutrient required by many tissues in the body. For more information about carbohydrates and ...
Mechanisms of transport through the Golgi complex
... Fig. 3. (A) Vesicle budding and fusion. Activation of a small G protein (such as Arf1 or Sar1) (red) by the exchange of GDP for GTP results in the recruitment of a coat complex (blue) to the membrane by the GTP-bound form of the G protein. Membrane curvature and sorting of cargo (yellow) into the fo ...
... Fig. 3. (A) Vesicle budding and fusion. Activation of a small G protein (such as Arf1 or Sar1) (red) by the exchange of GDP for GTP results in the recruitment of a coat complex (blue) to the membrane by the GTP-bound form of the G protein. Membrane curvature and sorting of cargo (yellow) into the fo ...
ppt - Chair of Computational Biology
... Each of the 400 possible pairs of amino acids is associated with a vector component representing the percentage of the primary sequence consisting of that pair. The program was reported as having a - 99.5% predictive accuracy at the GPCR versus non-GPCR level, - 97.3% accuracy at the Class level and ...
... Each of the 400 possible pairs of amino acids is associated with a vector component representing the percentage of the primary sequence consisting of that pair. The program was reported as having a - 99.5% predictive accuracy at the GPCR versus non-GPCR level, - 97.3% accuracy at the Class level and ...
Lesson Plan
... 8/28 structure and functions of different types of biomolecules including carbohydrates, lipids, proteins, and nucleic acids. ...
... 8/28 structure and functions of different types of biomolecules including carbohydrates, lipids, proteins, and nucleic acids. ...