Master Project Proposal Title: Enhancing reprogramming and
... adult somatic cells can be reprogrammed (named induced pluripotent stem cells – iPSC) by expressing a combination of 4 transcription factors (Oct4, Sox2, Klf4 and cMyc; 4-TFs). This discovery holds immense promise for clinical applications, as it implies that pluripotent cells can, in principle, be ...
... adult somatic cells can be reprogrammed (named induced pluripotent stem cells – iPSC) by expressing a combination of 4 transcription factors (Oct4, Sox2, Klf4 and cMyc; 4-TFs). This discovery holds immense promise for clinical applications, as it implies that pluripotent cells can, in principle, be ...
Biology Review
... 19. What kind of muscle causes the heart to beat? What makes this kind of muscle different from the muscles in the digestive system? ...
... 19. What kind of muscle causes the heart to beat? What makes this kind of muscle different from the muscles in the digestive system? ...
functions of the circulatory system
... FUNCTIONS OF THE CIRCULATORY SYSTEM The circulatory system has seven major functions: ...
... FUNCTIONS OF THE CIRCULATORY SYSTEM The circulatory system has seven major functions: ...
Stem Cells Murder or Miracle?
... “allows scientists to produce embryonic stem cells without using sperm to fertilize an egg.” ...
... “allows scientists to produce embryonic stem cells without using sperm to fertilize an egg.” ...
Scaria George Abstract 2015
... Natural Killer (NK) cells function as key effector cells of the innate immune system by providing a key role in host defense against malignancy and viral infection. NK cells also represent a useful tool for cellular therapy due to their ability to kill target cells in a human leukocyte antigen (HLA) ...
... Natural Killer (NK) cells function as key effector cells of the innate immune system by providing a key role in host defense against malignancy and viral infection. NK cells also represent a useful tool for cellular therapy due to their ability to kill target cells in a human leukocyte antigen (HLA) ...
Stem Cells
... Blastocyst – hollow ball of cells; early embryo Pluripotent – inside blastocyst; can develop into most types of cells (not the tissue around embryo) ...
... Blastocyst – hollow ball of cells; early embryo Pluripotent – inside blastocyst; can develop into most types of cells (not the tissue around embryo) ...
課堂簡報
... 24 genes were selected as candidates to induce pluripotency in somatic cells, For bcatenin, c-Myc, and Stat3, they used active forms, S33Y-b-catenin, T58A-c-Myc and Stat3-C, respectively. Because of the reported negative effect of Grb2 on pluripotency, they also included its dominant-negative mutan ...
... 24 genes were selected as candidates to induce pluripotency in somatic cells, For bcatenin, c-Myc, and Stat3, they used active forms, S33Y-b-catenin, T58A-c-Myc and Stat3-C, respectively. Because of the reported negative effect of Grb2 on pluripotency, they also included its dominant-negative mutan ...
Investigation 1 “Living Cells” Big Ideas
... in contact with cells. Blood returns to the heart in veins. 4. How do cells in humans get the nutrients they need? a. The digestive system reduces food to nutrients. Nutrients pass out of the digestive system into the bloodstream for transport to all the cells. 5. How does the digestive system work? ...
... in contact with cells. Blood returns to the heart in veins. 4. How do cells in humans get the nutrients they need? a. The digestive system reduces food to nutrients. Nutrients pass out of the digestive system into the bloodstream for transport to all the cells. 5. How does the digestive system work? ...
Stem Cells - inetTeacher
... Adult Stem Cells • Multipotent: can become only a few cell types • Replace constantly dying cells (skin, intestines, blood) • Scientists are able to turn adult stem cells into embryonic stem cells by inserting genes into them! – This became necessary when embryonic stem cell research was banned in ...
... Adult Stem Cells • Multipotent: can become only a few cell types • Replace constantly dying cells (skin, intestines, blood) • Scientists are able to turn adult stem cells into embryonic stem cells by inserting genes into them! – This became necessary when embryonic stem cell research was banned in ...
Living cells can sense their physical
... stem cells (ESCs) in poly ethylene glycol diacrylate (PEGDA) hydrogels. These hydrogels are being fabricated using a three dimensional printer - stereolithography apparatus (SLA). SLA offers several advantages like multi-cell, multi-material fabrication and capability to encapsulate cells during the ...
... stem cells (ESCs) in poly ethylene glycol diacrylate (PEGDA) hydrogels. These hydrogels are being fabricated using a three dimensional printer - stereolithography apparatus (SLA). SLA offers several advantages like multi-cell, multi-material fabrication and capability to encapsulate cells during the ...
lecture notes
... begin next week, the week of Jan 26. 3. Your grade will be based on a total of 500 pts: 100 pts for each midterm, 100 pts for a comprehensive final, and 100 pts for participation and quizzes in the discussion section. Note that the final exam period will consist of two parts. Midterm 3 (100 pts) fol ...
... begin next week, the week of Jan 26. 3. Your grade will be based on a total of 500 pts: 100 pts for each midterm, 100 pts for a comprehensive final, and 100 pts for participation and quizzes in the discussion section. Note that the final exam period will consist of two parts. Midterm 3 (100 pts) fol ...
Data Collection
... Derivation normally results in embryo destruction Prior to March 9, 2009, only a limited number of lines were available for federally funded research Risk of tumors (teratomas) from transplanting undifferentiated cells ...
... Derivation normally results in embryo destruction Prior to March 9, 2009, only a limited number of lines were available for federally funded research Risk of tumors (teratomas) from transplanting undifferentiated cells ...
Student worksheet for prokaryotic, animal and plant cells
... Student—please print this worksheet and complete it as you interact with the tutorial. The completed worksheet should be turned in to your assigned teacher. Tutorial: Comparison of prokaryotic, animal and plant cells 1. Plants and animals have eukaryotic cells. What is the other type of cell in this ...
... Student—please print this worksheet and complete it as you interact with the tutorial. The completed worksheet should be turned in to your assigned teacher. Tutorial: Comparison of prokaryotic, animal and plant cells 1. Plants and animals have eukaryotic cells. What is the other type of cell in this ...
Introduction to Stem Cells
... most useful to treat human disease? Give a reason for your answer. ...
... most useful to treat human disease? Give a reason for your answer. ...
Method for producing autonomously contracting cardiac muscle
... the inability of mature heart muscle cells (cardiomyocytes) to divide and replace damaged heart muscle. Since the therapeutic use of embryonic cardiomyocytes is prohibited in most countries, adult human stem cells could represent an alternative for regenerative medicine. Adult stem cells of differen ...
... the inability of mature heart muscle cells (cardiomyocytes) to divide and replace damaged heart muscle. Since the therapeutic use of embryonic cardiomyocytes is prohibited in most countries, adult human stem cells could represent an alternative for regenerative medicine. Adult stem cells of differen ...
Artificial insemination
... the inner cell mass of the blastocyst (ES) • We can use the excess embryos of IVF to obtain ES. • And by the means of SCNT we can produce as many blastocysts (ES) as we want. • And there is a further possible source of pluripotent stem cells, since those can also be isolated from aborted fetuses, mo ...
... the inner cell mass of the blastocyst (ES) • We can use the excess embryos of IVF to obtain ES. • And by the means of SCNT we can produce as many blastocysts (ES) as we want. • And there is a further possible source of pluripotent stem cells, since those can also be isolated from aborted fetuses, mo ...
Stem Cells: What`s the Big Deal
... Name: _________________________________________ Why are stem cells important? Web page sources: ...
... Name: _________________________________________ Why are stem cells important? Web page sources: ...
Early Embryonic Development
... • m-RNA is made from the active genes • m-RNA moves to the ribosomes; the ribosomes read the m-RNA and the appropriate protein is made • The protein can permanently alter the structure and function of cells ...
... • m-RNA is made from the active genes • m-RNA moves to the ribosomes; the ribosomes read the m-RNA and the appropriate protein is made • The protein can permanently alter the structure and function of cells ...
Slide 1
... Direct microinjection of a chosen gene construct from another member of the same species or from a different species, into the pronucleus of a fertilized ovum. ...
... Direct microinjection of a chosen gene construct from another member of the same species or from a different species, into the pronucleus of a fertilized ovum. ...
Induced pluripotent stem cell
Induced pluripotent stem cells (also known as iPS cells or iPSCs) are a type of pluripotent stem cell that can be generated directly from adult cells. The iPSC technology was pioneered by Shinya Yamanaka’s lab in Kyoto, Japan, who showed in 2006 that the introduction of four specific genes encoding transcription factors could convert adult cells into pluripotent stem cells. He was awarded the 2012 Nobel Prize along with Sir John Gurdon ""for the discovery that mature cells can be reprogrammed to become pluripotent."" Pluripotent stem cells hold great promise in the field of regenerative medicine. Because they can propagate indefinitely, as well as give rise to every other cell type in the body (such as neurons, heart, pancreatic, and liver cells), they represent a single source of cells that could be used to replace those lost to damage or disease.The most well-known type of pluripotent stem cell is the embryonic stem cell. However, since the generation of embryonic stem cells involves destruction (or at least manipulation) of the pre-implantation stage embryo, there has been much controversy surrounding their use. Further, because embryonic stem cells can only be derived from embryos, it has so far not been feasible to create patient-matched embryonic stem cell lines.Since iPSCs can be derived directly from adult tissues, they not only bypass the need for embryos, but can be made in a patient-matched manner, which means that each individual could have their own pluripotent stem cell line. These unlimited supplies of autologous cells could be used to generate transplants without the risk of immune rejection. While the iPSC technology has not yet advanced to a stage where therapeutic transplants have been deemed safe, iPSCs are readily being used in personalized drug discovery efforts and understanding the patient-specific basis of disease.Depending on the methods used, reprogramming of adult cells to obtain iPSCs may pose significant risks that could limit their use in humans. For example, if viruses are used to genomically alter the cells, the expression of oncogenes (cancer-causing genes) may potentially be triggered. In February 2008, scientists announced the discovery of a technique that could remove oncogenes after the induction of pluripotency, thereby increasing the potential use of iPS cells in human diseases. In April 2009, it was demonstrated that generation of iPS cells is possible without any genetic alteration of the adult cell: a repeated treatment of the cells with certain proteins channeled into the cells via poly-arginine anchors was sufficient to induce pluripotency. The acronym given for those iPSCs is piPSCs (protein-induced pluripotent stem cells).