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Measuring the mechanical properties of plant cells by combining
Measuring the mechanical properties of plant cells by combining

... Several models have been proposed that describe indentation experiments at different scales. A  mathematical model that is often used to interpret data from AFM experiments is the Hertz model (Lin et al., 2007). This assumes that indentations are small enough to only probe elastic properties of the ...
performance of nonlinear visual units in ocular hypertension
performance of nonlinear visual units in ocular hypertension

... Glovinsky et al., 1991) that project to the magnocellular layers of the lateral geniculate nucleus (LGN). In the primate LGN, the two magnocellular layers lie ventral to the parvocellular layers, which in different species may be two or four in number (for a review see Henry, 1991). Most, if not all ...
Female Gametophyte Development in Flowering Plants
Female Gametophyte Development in Flowering Plants

... megaspores (diploid) (60). However, only the chalazal megaspore, but not the micropylar megaspore, expresses functional megasporespecific markers. This indicates that only the chalazal megaspore is functional and suggests a position-dependent mechanism. It also implies that a decision on cell fate ha ...
and Function Activation Sulfenic Acid Formation for T Cell The
and Function Activation Sulfenic Acid Formation for T Cell The

... Reactive oxygen intermediates (ROI) generated in response to receptor stimulation play an important role in mediating cellular responses. We have examined the importance of reversible cysteine sulfenic acid formation in naive CD8ⴙ T cell activation and proliferation. We observed that, within minutes ...
Science Cell Parts Project
Science Cell Parts Project

... Choose one of the following cell projects. Your completed project must have descriptions of the organelles that are listed on the next page. A. Cell Factory: In a Shoebox. The Cell has many organelles. Each organelle has its own specific function or job. Many times the Eukaryotic Cell has been descr ...
Chapter 1: The Microbial World and You
Chapter 1: The Microbial World and You

... staining between Gram-positive and Gram-negative cells? Gram-positive cells have very thick peptidoglycan cell walls, whereas gram-negative cells have very thin cell walls. Crystal violet easily penetrates both cell types. Because of its larger size, the crystal violet-iodine complex [CV-I] is not ...
The Patch-Clamp Method
The Patch-Clamp Method

... patch of membrane, in relation to the patch electrode -The formation of an outside-out patch begins with a whole-cell recording configuration. After the whole-cell configuration is formed, the electrode is slowly withdrawn from the cell, allowing a bulb of membrane to bleb out from the cell. When th ...
Cell and Molecular Biology - 外文文献下载
Cell and Molecular Biology - 外文文献下载

... The cell membrane is an organized sea of lipid in a fluid state, a nonaqueous dynamic compartment of cells. The cell membranes are assembled from four major components: a lipid bilayer, membrane proteins, sugar residues, and a network of supporting fibers. The basic structure of a cell membrane is a ...
Remapping by hippocampal place cells
Remapping by hippocampal place cells

... Michael I. Anderson, Robin Hayman, Subhojit Chakraborty and Kathryn J. Jeffery Department of Psychology, University College London, 26 Bedford Way, London WC1H OAP, UK ...
Systematic profiling of cellular phenotypes with spotted cell
Systematic profiling of cellular phenotypes with spotted cell

... participating in the mating-pheromone response pathway. Strains defective in the pathway fail to arrest growth when treated with alpha factor, unlike wild-type cells. The histograms report the average results of two or three replicate growth assays for (a) 28 strains containing deletions of genes kn ...
Isolation and Characterization of Monoclonal Antibodies Directed
Isolation and Characterization of Monoclonal Antibodies Directed

... of total tobacco cell culture protein extracts using a combination of nonequilibrium pH-gradient gel electrophoresis and SDS-PAGE. Silver staining revealed many well-resolved molecules (Fig. 1 A). Western blotting of these gels identified a single molecular species bearing the ll.D2 epitope (Fig. 1 ...
The Bacterial Cytoskeleton
The Bacterial Cytoskeleton

... The Z ring is also thought to play an active role during the invagination of the division septum, generating a pinching force on the cell membrane. Recently, Erickson and colleagues showed that when purified FtsZ was incorporated into lipid vesicles in vitro, it could assemble into Z rings and produ ...
7-2 Eukaryotic Cell Structure
7-2 Eukaryotic Cell Structure

... There are two types of ER—rough and smooth. The portion of the ER involved in protein synthesis is called rough endoplasmic reticulum, or rough ER. Ribosomes are found on the surface of rough ER. Rough ER is abundant in cells that produce large amounts of protein for export. ...
Structures Macromolecular - CNB
Structures Macromolecular - CNB

... virus-related complexes are under detailed analysis using correlative approaches, including X-ray crystallography and advanced cryo-electron 3D-microscopy methods. These studies involve extensive development of image acquisition and processing tools to achieve improved resolution. The department has ...
Structural Analysis and Functional Implications of
Structural Analysis and Functional Implications of

... from eIF4E at the 50 cap and allowing eIF4E interaction with eIF4G and the nucleation of a preinitiation complex (1). mTORC1 also phosphorylates S6K1 (S6 kinase 1), and this phosphorylation event [at Thr389; (10)] primes S6K1 for further phosphorylation and activation. S6K1 in turn phosphorylates, a ...
Mudskipper gill MR cell ion-transport proteins
Mudskipper gill MR cell ion-transport proteins

... ammonia concentrations. To test whether these Na+/K+-ATPase and a Na+/K+/2Cl− cotransporter. A branchial MR cells are the sites of active ammonia proportion of the ammonia eliminated by P. schlosseri elimination, we used an immunological approach to involves carbonic anhydrase activity and is not de ...
Quality control in the initiation of eukaryotic DNA replication
Quality control in the initiation of eukaryotic DNA replication

... to G1 phase, when origins cannot fire because CDKs are absent, and activation of CDK at the end of G1 phase triggers initiation from licensed origins, and prevents the re-assembly of pre-RCs at origins that have fired. The role for CDK in preventing licensing outside of G1 phase has been conserved i ...
Contents - Hodder Education
Contents - Hodder Education

... A bacterium does not possess membrane-bound organelles. For example, it lacks a nucleus containing threadlike chromosomes. Instead, its genetic material takes the form of a single circular chromosome and several smaller circular plasmids. The structure and composition of the cell wall of a bacterium ...
Nuclear pore interactions with the genome
Nuclear pore interactions with the genome

... Varun Sood and Jason H Brickner Within the nucleus, chromatin is functionally organized into distinct nuclear compartments. The nuclear periphery, containing Nuclear Pore Complexes (NPCs), plays an important role in the spatial organization of chromatin and in transcriptional regulation. The role of ...
Midbodies and phragmoplasts: analogous structures
Midbodies and phragmoplasts: analogous structures

... phragmoplast and growing similarities between these two structures. The midbody In animal cells, after sister chromosomes have separated, the remaining non-kinetochore, overlapping MTs form a structure called the spindle midzone (Glossary). At this time, the actomyosin ring begins to assemble and co ...
Differential Diagnosis: Ataxia (Drug-Induced)
Differential Diagnosis: Ataxia (Drug-Induced)

... • Pathophysiology: Affects the function of the anterior lobe of the cerebellum (or spinocerebellum), which influences our coordination of movement. Fluorouracil (5-FU) • Associations: Reports of being treated for cancer • Pathophysiology: While neurotoxicity is overall rare, it does appear to be dos ...
Inhibition of Phorbol Ester-Induced Monocytic
Inhibition of Phorbol Ester-Induced Monocytic

... (Fig 3). These findings indicated that dexamethasone inhibits pathways involved in the induction of c-fms and T N F expression, but not in the down-regulation of c-myc expression. To further analyze the effects of dexamethasone on c-fms expression, we measured the rate of c-fms gene transcription in ...
Mutations in the VPS45 gene, a SEC1 homologue, result in vacuolar
Mutations in the VPS45 gene, a SEC1 homologue, result in vacuolar

... endosomal membranes. Significantly, this fraction lacks ER, vacuole and plasma membranes. Overexpression of Vps45p saturates the sites with which Vps45p associates. A vps45 null mutant accumulates vesicles, many of which were found to be present in large clusters. This accumulation of potential tran ...
Molecular General and Genetics
Molecular General and Genetics

... Interestingly, we found that the transcript of the mSOD1 transcript from tuberous root was smaller than that found in other tissues (Fig. 4). This smaller transcript may be produced by alternative splicing. It has been reported that synthesis of ascorbate peroxidases is controlled by alternative spl ...
Ras Part II
Ras Part II

... Constitutively active Ras ...
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Amitosis

Amitosis (a- + mitosis) is absence of mitosis, the usual form of cell division in the cells of eukaryotes. There are several senses in which eukaryotic cells can be amitotic. One refers to capability for non-mitotic division and the other refers to lack of capability for division. In one sense of the word, which is now mostly obsolete, amitosis is cell division in eukaryotic cells that happens without the usual features of mitosis as seen on microscopy, namely, without nuclear envelope breakdown and without formation of mitotic spindle and condensed chromosomes as far as microscopy can detect. However, most examples of cell division formerly thought to belong to this supposedly ""non-mitotic"" class, such as the division of unicellular eukaryotes, are today recognized as belonging to a class of mitosis called closed mitosis. A spectrum of mitotic activity can be categorized as open, semi-closed, and closed mitosis, depending on the fate of the nuclear envelope. An exception is the division of ciliate macronucleus, which is not mitotic, and the reference to this process as amitosis may be the only legitimate use of the ""non-mitotic division"" sense of the term today. In animals and plants which normally have open mitosis, the microscopic picture described in the 19th century as amitosis most likely corresponded to apoptosis, a process of programmed cell death associated with fragmentation of the nucleus and cytoplasm. Relatedly, even in the late 19th century cytologists mentioned that in larger life forms, amitosis is a ""forerunner of degeneration"".Another sense of amitotic refers to cells of certain tissues that are usually no longer capable of mitosis once the organism has matured into adulthood. In humans this is true of various muscle and nerve tissue types; if the existing ones are damaged, they cannot be replaced with new ones of equal capability. For example, cardiac muscle destroyed by heart attack and nerves destroyed by piercing trauma usually cannot regenerate. In contrast, skin cells are capable of mitosis throughout adulthood; old skin cells that die and slough off are replaced with new ones. Human liver tissue also has a sort of dormant regenerative ability; it is usually not needed or expressed but can be elicited if needed.
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