SepF, a novel FtsZ-interacting protein required for a late step in cell
... Pspac promoter. Strain 4054 was similar except that the 165 bp potential promoter region remained in position upstream of ylmD. When an ezrA mutation was introduced into these strains, the resulting transformants grew normally in the presence of IPTG. However, transformants from strain 4055 became e ...
... Pspac promoter. Strain 4054 was similar except that the 165 bp potential promoter region remained in position upstream of ylmD. When an ezrA mutation was introduced into these strains, the resulting transformants grew normally in the presence of IPTG. However, transformants from strain 4055 became e ...
T cell metabolism drives immunity
... modulation of gene expression and the acquisition of new functions.These changes range from increased production of cytokines and cytolytic molecules to the ability to undergo cell division and migration. Intimately integrated into this program of activation is the regulation of cellular metabolism. ...
... modulation of gene expression and the acquisition of new functions.These changes range from increased production of cytokines and cytolytic molecules to the ability to undergo cell division and migration. Intimately integrated into this program of activation is the regulation of cellular metabolism. ...
BYSTANDER HELP IN PRIMARY IMMUNE RESPONSES IN
... to show in vivo or for protein-bound indicator antigens (21-24). Thus, it has been proposed that this mechanism of T cell-B cell collaboration may be of little importance in vivo (25, 26). The murine immune response to heterologous insulins is controlled by H-2linked immune response (Ir) genes (27-3 ...
... to show in vivo or for protein-bound indicator antigens (21-24). Thus, it has been proposed that this mechanism of T cell-B cell collaboration may be of little importance in vivo (25, 26). The murine immune response to heterologous insulins is controlled by H-2linked immune response (Ir) genes (27-3 ...
Gene transcription is coordinated with, but not dependent on, cell
... between the last cell division and the next division based on its cell count. We considered all embryos from a sample within an interval, equally spaced them in time and ordered them as increasing or decreasing, or randomly, based on their expression level and depending on which behavior we assumed ...
... between the last cell division and the next division based on its cell count. We considered all embryos from a sample within an interval, equally spaced them in time and ordered them as increasing or decreasing, or randomly, based on their expression level and depending on which behavior we assumed ...
Cancer`s Trojan Horse
... • Cancer bearing mice that received it lived twice as long as mice treated with the non-targeted drug • No signs of toxicity to normal tissues ...
... • Cancer bearing mice that received it lived twice as long as mice treated with the non-targeted drug • No signs of toxicity to normal tissues ...
2281-MC-025 Bax 6A7 for pdf
... Description: The Bcl-2 family of proteins plays a crucial role in the regulation of cell death in many eukaryotic systems. Bax has been shown to redistribute from the cytosol to the mitochondria during apoptosis, and overexpression of Bax can accelerate cell death. Coregulation of Bax dimer formatio ...
... Description: The Bcl-2 family of proteins plays a crucial role in the regulation of cell death in many eukaryotic systems. Bax has been shown to redistribute from the cytosol to the mitochondria during apoptosis, and overexpression of Bax can accelerate cell death. Coregulation of Bax dimer formatio ...
PDF - The Journal of Immunology
... tion of glycolipid Ags presented by CD1d (3, 4). These Ags can be of exogenous microbial origin or be endogenous self-antigens presented at elevated levels and in an inflammatory milieu (5, 6). Glucosylceramide (GlcCer) with a 24:1 N-acyl chain paired with a d18:1 sphingosine base is one such endoge ...
... tion of glycolipid Ags presented by CD1d (3, 4). These Ags can be of exogenous microbial origin or be endogenous self-antigens presented at elevated levels and in an inflammatory milieu (5, 6). Glucosylceramide (GlcCer) with a 24:1 N-acyl chain paired with a d18:1 sphingosine base is one such endoge ...
Biology 376 Animal Development
... The stories are in every newspaper: cloning, stem cells, genetic engineering, in vitro fertilization, cancer therapies, organ regeneration, and protocols for prolonging our lifespan. In the past five years, developmental biology has usurped a place formerly occupied by science fiction… This ability ...
... The stories are in every newspaper: cloning, stem cells, genetic engineering, in vitro fertilization, cancer therapies, organ regeneration, and protocols for prolonging our lifespan. In the past five years, developmental biology has usurped a place formerly occupied by science fiction… This ability ...
Massive Expansion of Antigen-Specific CD8 T Cells during an Acute
... not all of which recognize LCMV antigens (Yang and Welsh, 1986; Nahill and Welsh, 1993). In addition to their expanded numbers, most CD81 T cells during the acute immune response to LCMV show signs of activation. They are enlarged; have elevated surface expression of CD11a, CD11b, CD44, CD49d, and t ...
... not all of which recognize LCMV antigens (Yang and Welsh, 1986; Nahill and Welsh, 1993). In addition to their expanded numbers, most CD81 T cells during the acute immune response to LCMV show signs of activation. They are enlarged; have elevated surface expression of CD11a, CD11b, CD44, CD49d, and t ...
Vps41 Phosphorylation and the Rab Ypt7 Control the Targeting of
... us to analyze yck3⌬ cells by electron microscopy. In contrast to wild-type cells, yck3⌬ cells contain elongated structures proximal to the vacuole (Figure 1B, A and B), which are in part reminiscent of the class E endosomes observed upon loss of ESCRT subunits (Rieder et al., 1996). The tubular stru ...
... us to analyze yck3⌬ cells by electron microscopy. In contrast to wild-type cells, yck3⌬ cells contain elongated structures proximal to the vacuole (Figure 1B, A and B), which are in part reminiscent of the class E endosomes observed upon loss of ESCRT subunits (Rieder et al., 1996). The tubular stru ...
Genetic Analysis of the Yeast Cytoskeleton.
... in the cell cycle and grows steadily as the cell cycle progresses, remaining attached to the mother cell by a short neck region. Near the end of the DNA synthetic period, the nucleus migrates toward the neck. After DNAsynthesis is completedthe nucleus elongates symmetrically into the mother and daug ...
... in the cell cycle and grows steadily as the cell cycle progresses, remaining attached to the mother cell by a short neck region. Near the end of the DNA synthetic period, the nucleus migrates toward the neck. After DNAsynthesis is completedthe nucleus elongates symmetrically into the mother and daug ...
Phorbol Esters Alter Cell Fate during Development of Sea Urchin
... The morphological changes observed during gastrulation as a result of treatment with phorbol ester during cleavage led us to investigate the changes in gene expression in TPAtreated embryos, as assessed by RNA accumulation. A number of genes have been isolated in sea urchins that by the early gastru ...
... The morphological changes observed during gastrulation as a result of treatment with phorbol ester during cleavage led us to investigate the changes in gene expression in TPAtreated embryos, as assessed by RNA accumulation. A number of genes have been isolated in sea urchins that by the early gastru ...
Receptor-Mediated Entry by Equine Infectious Anemia Virus Utilizes
... of specific agents on virus production, drug treatments were performed as described above, except that the postinfection period was extended to 1 to 6 days. To prevent sequential rounds of virus infection from infected cells, the reverse transcription inhibitor 3⬘-azido-3⬘-deoxythymidine (AZT; Sigma ...
... of specific agents on virus production, drug treatments were performed as described above, except that the postinfection period was extended to 1 to 6 days. To prevent sequential rounds of virus infection from infected cells, the reverse transcription inhibitor 3⬘-azido-3⬘-deoxythymidine (AZT; Sigma ...
Drosophila follicle cells are patterned by multiple
... formation. Dl homozygous clones were induced in FRT82B DlM1/FRT82B GFP females, and are marked by the loss of GFP (green). (A,A⬘) Germline cysts completely surrounded by Dl-mutant follicle cells fuse with the neighboring anterior cyst, but have a normal posterior stalk (arrowhead in A⬘). Fas3 (red) ...
... formation. Dl homozygous clones were induced in FRT82B DlM1/FRT82B GFP females, and are marked by the loss of GFP (green). (A,A⬘) Germline cysts completely surrounded by Dl-mutant follicle cells fuse with the neighboring anterior cyst, but have a normal posterior stalk (arrowhead in A⬘). Fas3 (red) ...
Standard PDF - Wiley Online Library
... nism of regulation in which ABC-transporter-like complexes regulate the activities of specific endopeptidases (Sham et al., 2011; Yang et al., 2011). The ABC transporter corresponds to a previously described cell division factor called FtsEX. It seems that the ATPase activity of the nucleotide-bindi ...
... nism of regulation in which ABC-transporter-like complexes regulate the activities of specific endopeptidases (Sham et al., 2011; Yang et al., 2011). The ABC transporter corresponds to a previously described cell division factor called FtsEX. It seems that the ATPase activity of the nucleotide-bindi ...
A Novel Nuclear Pore Protein Nup82p Which
... of nuclear protein import is beginning to emerge (Powers and Forbes, 1994), very little is known about the following steps at the NPC, in particular which nuclear pore proteins are involved in the actual translocation process and how they act. It is likely that some of the cytosolic/nuclear factors ...
... of nuclear protein import is beginning to emerge (Powers and Forbes, 1994), very little is known about the following steps at the NPC, in particular which nuclear pore proteins are involved in the actual translocation process and how they act. It is likely that some of the cytosolic/nuclear factors ...
Structural and functional characterization of the promoter regions of
... upon protein-protein interactions among various NF-KB factors and among these and inhibitory molecules (IKBS) which control the subcellular (nuclear/cytoplasmic) localization of the active complexes. In response to extracellular stimuli, including cytokines, mitogens and viruses, the NF-KB complexes ...
... upon protein-protein interactions among various NF-KB factors and among these and inhibitory molecules (IKBS) which control the subcellular (nuclear/cytoplasmic) localization of the active complexes. In response to extracellular stimuli, including cytokines, mitogens and viruses, the NF-KB complexes ...
6 December 2009
... Various methods that have been used to estimate the thickness of the cleft width formed between different cell types and substrates revealed that the cleft thickness ranges between 0 and 150 nm, averaging at 30 – 40 nm. For example, fluorescence interferencecontrast microscopy of neurons and fibroblas ...
... Various methods that have been used to estimate the thickness of the cleft width formed between different cell types and substrates revealed that the cleft thickness ranges between 0 and 150 nm, averaging at 30 – 40 nm. For example, fluorescence interferencecontrast microscopy of neurons and fibroblas ...
TGFβ/activin/nodal signaling is necessary for the maintenance of
... Embryonic stem cells are a population of multipotent, selfrenewing cells that are derived from the epiblast of mammalian blastocyst embryos and that retain this developmental identity even after prolonged culture in vitro (Rossant, 2001). ES cells can be induced to differentiate to functional cell t ...
... Embryonic stem cells are a population of multipotent, selfrenewing cells that are derived from the epiblast of mammalian blastocyst embryos and that retain this developmental identity even after prolonged culture in vitro (Rossant, 2001). ES cells can be induced to differentiate to functional cell t ...
Becke S, Fabre-Mersseman V, Aue S, Auerochs S
... (Fig. 1a). To analyse whether the expression of the pp65– VM1 fusion protein was reduced in comparison to wild-type (wt)-pp65, Western blot analysis of infected cells was performed (Fig. 1b). No reduction of the steady-state level of the fusion protein at 4 days post-infection (p.i.) could be detect ...
... (Fig. 1a). To analyse whether the expression of the pp65– VM1 fusion protein was reduced in comparison to wild-type (wt)-pp65, Western blot analysis of infected cells was performed (Fig. 1b). No reduction of the steady-state level of the fusion protein at 4 days post-infection (p.i.) could be detect ...
Drug development in oncology assisted by - HAL
... Cancer is a major public health problem. It is the leading cause of death in the world, before cardiovascular diseases. Significant progress has been made in the last decade, but the improvement of diagnosis, with early and accurate detection of tumors, as well as the development of innovative targe ...
... Cancer is a major public health problem. It is the leading cause of death in the world, before cardiovascular diseases. Significant progress has been made in the last decade, but the improvement of diagnosis, with early and accurate detection of tumors, as well as the development of innovative targe ...
ch_03_lecture_outline_a
... and extracellular matrix (ECM) Elements of the cytoskeleton (cell’s internal supports) and the extracellular matrix (fibers and other substances outside the cell) may be anchored to membrane proteins, which help maintain cell shape and fix the location of certain membrane proteins. Others play a rol ...
... and extracellular matrix (ECM) Elements of the cytoskeleton (cell’s internal supports) and the extracellular matrix (fibers and other substances outside the cell) may be anchored to membrane proteins, which help maintain cell shape and fix the location of certain membrane proteins. Others play a rol ...
Links between genome replication and chromatin landscapes
... requires thousands of cell divisions to transform the unicellular zygote into the adult body. This is particularly relevant in the case of plants, in which organogenesis occurs entirely in a post-embryonic manner. Remarkably, the integrity of the genetic material of every cell must be maintained thr ...
... requires thousands of cell divisions to transform the unicellular zygote into the adult body. This is particularly relevant in the case of plants, in which organogenesis occurs entirely in a post-embryonic manner. Remarkably, the integrity of the genetic material of every cell must be maintained thr ...
Chapter_12
... propagation delay, switching delay Assume propagation at two-thirds speed of light If source and destination on opposite sides of USA, propagation time ~ 48x10-3 seconds Given implicit congestion control, by the time dropped cell notification has reached source, 7.2x106 bits have been transmitted ...
... propagation delay, switching delay Assume propagation at two-thirds speed of light If source and destination on opposite sides of USA, propagation time ~ 48x10-3 seconds Given implicit congestion control, by the time dropped cell notification has reached source, 7.2x106 bits have been transmitted ...
Poor Devils: The Plight of the Tasmanian Devils
... You may recall the cartoon character the Tasmanian Devil of Looney Tunes fame from your childhood memories. It may surprise you to learn that this character is based on a real animal that lives in Tasmania, a large island-state off the southeast coast of Australia. The devils used to be found in Aus ...
... You may recall the cartoon character the Tasmanian Devil of Looney Tunes fame from your childhood memories. It may surprise you to learn that this character is based on a real animal that lives in Tasmania, a large island-state off the southeast coast of Australia. The devils used to be found in Aus ...