Optical measurement of cell membrane tension
... portion of the interfering beams before they reach the CCD. At a plane conjugate to the image (CCD) plane, we place an amplitude grating, which has the same period as the interferogram. Due to this spatial matching, each diffraction order n produced by the sample beam overlaps with the order n-1 of ...
... portion of the interfering beams before they reach the CCD. At a plane conjugate to the image (CCD) plane, we place an amplitude grating, which has the same period as the interferogram. Due to this spatial matching, each diffraction order n produced by the sample beam overlaps with the order n-1 of ...
Nuclear Synthesis of Cytoplasmic Ribonucleic Acid
... The enucleation technique has been applied to Amoeba proteus by several laboratories in attempts to determine whether the cytoplasm is capable of nucleusindependent ribonucleic acid synthesis. This cell is very convenient for micrurgy, but its use requires a thorough starvation period to eliminate t ...
... The enucleation technique has been applied to Amoeba proteus by several laboratories in attempts to determine whether the cytoplasm is capable of nucleusindependent ribonucleic acid synthesis. This cell is very convenient for micrurgy, but its use requires a thorough starvation period to eliminate t ...
The Arabidopsis Exocyst Complex Is Involved in Cytokinesis and
... bait and prey and weakly interacted as bait with SEC15b. On the other hand, the C-terminal domain of EXO84b exhibited weak interactions with itself and, as bait, with SEC15b (Figure 1A). We further demonstrated the association of EXO84b with other exocyst subunits by coimmunoprecipitation in transge ...
... bait and prey and weakly interacted as bait with SEC15b. On the other hand, the C-terminal domain of EXO84b exhibited weak interactions with itself and, as bait, with SEC15b (Figure 1A). We further demonstrated the association of EXO84b with other exocyst subunits by coimmunoprecipitation in transge ...
Mary Ann Thompson Arildsen, M
... Due to the retirement of our previous MTIII hematology technologist, Sally Pankau, we are interviewing for two new MTIII hematology technologists. One will be focused on teaching, and the other will be focused on instrumentation. We are very fortunate to have 4 strong internal candidates to choose ...
... Due to the retirement of our previous MTIII hematology technologist, Sally Pankau, we are interviewing for two new MTIII hematology technologists. One will be focused on teaching, and the other will be focused on instrumentation. We are very fortunate to have 4 strong internal candidates to choose ...
Membrane Protein Sorting in the Yeast Secretory Pathway
... bodies that are similar to those containing Kex2p in size, abundance, and distribution. In this paper, a combined gene fusion and mutational analysis was used to show that no single domain of DPAP B is required for vacuolar localization. Furthermore, both overproduction of DPAP A and a mutation in t ...
... bodies that are similar to those containing Kex2p in size, abundance, and distribution. In this paper, a combined gene fusion and mutational analysis was used to show that no single domain of DPAP B is required for vacuolar localization. Furthermore, both overproduction of DPAP A and a mutation in t ...
Surface Charge Distribution on the Endothelial Cell of Liver Sinusoids
... ANIONIC MARKERS : 30 s to 3 min after in vivo or in situ injection of NF, the endothelial cells of the mouse liver sinusoids bound this marker only in the coated microdomains of their plasma membrane (Fig. 1) . NF was also contained in coated vesicles and in some small uncoated vesicles (-200nm-diam ...
... ANIONIC MARKERS : 30 s to 3 min after in vivo or in situ injection of NF, the endothelial cells of the mouse liver sinusoids bound this marker only in the coated microdomains of their plasma membrane (Fig. 1) . NF was also contained in coated vesicles and in some small uncoated vesicles (-200nm-diam ...
Quaternary ammonium surfactant structure determines selective
... Results: QAS toxicity normalized relative to surfactant critical micelle concentration showed n-dodecylpyridinium bromide (C12PB) to be the most effective, with a therapeutic index of ∼10 for an MDR strain of Escherichia coli and .20 for Neisseria gonorrhoeae after 1 h of exposure. Three modes of QA ...
... Results: QAS toxicity normalized relative to surfactant critical micelle concentration showed n-dodecylpyridinium bromide (C12PB) to be the most effective, with a therapeutic index of ∼10 for an MDR strain of Escherichia coli and .20 for Neisseria gonorrhoeae after 1 h of exposure. Three modes of QA ...
Mapping of Lipid-‐Binding Proteins and Their Ligandability in Cells
... (5-lipoxygenase; 5-LO), and are thus converted into one of four families of eicosanoids: the prostaglandins (PGs), rom the extracellular side of the membrane prostacyclins, thromboxanes (TXs) and leukotrienes. Eicosanoids short half-life, ranging from R. et. al. Nature 2007, 4at 50, ...
... (5-lipoxygenase; 5-LO), and are thus converted into one of four families of eicosanoids: the prostaglandins (PGs), rom the extracellular side of the membrane prostacyclins, thromboxanes (TXs) and leukotrienes. Eicosanoids short half-life, ranging from R. et. al. Nature 2007, 4at 50, ...
Activation of the p75 Neurotrophin Receptor through Conformational
... cells (Figure 1A). At high levels of expression in COS-7 cells, comparable amounts of cell surface p75NTR monomers and disulphide-linked dimers could be detected under nonreducing conditions (Figure 1A). However, the proportion of disulphidelinked p75NTR dimers in transfected cells depended upon the ...
... cells (Figure 1A). At high levels of expression in COS-7 cells, comparable amounts of cell surface p75NTR monomers and disulphide-linked dimers could be detected under nonreducing conditions (Figure 1A). However, the proportion of disulphidelinked p75NTR dimers in transfected cells depended upon the ...
Biology placement flyer and study guide
... • Be able to describe the properties of the four groups of macromolecules and give examples of each • Review the first and second laws of thermodynamics and how living systems conform to these laws Cell Structure and Function • Review the concepts of evolution and natural selection • Describe the st ...
... • Be able to describe the properties of the four groups of macromolecules and give examples of each • Review the first and second laws of thermodynamics and how living systems conform to these laws Cell Structure and Function • Review the concepts of evolution and natural selection • Describe the st ...
Taxol-stabilized Microtubules Can Position the
... positioning, with a subpopulation of relatively stable microtubules interacting with the equatorial cortex to promote furrow formation, and dynamic microtubules at the polar regions helping to suppress ectopic furrowing (Canman et al., 2003). Therefore, microtubule dynamics, rather than their locati ...
... positioning, with a subpopulation of relatively stable microtubules interacting with the equatorial cortex to promote furrow formation, and dynamic microtubules at the polar regions helping to suppress ectopic furrowing (Canman et al., 2003). Therefore, microtubule dynamics, rather than their locati ...
Oligomerization of Epidermal Growth Factor Receptors on A431
... EGF (46); the activation kinetics using solubilized receptors have a second order dependence on receptor concentration (57); and chemical cross-linking studies confirm the occurrence of EGF-induced (13, 18) or covalently stabilized (45) receptor dimerization. Most of the studies of the E G F R have ...
... EGF (46); the activation kinetics using solubilized receptors have a second order dependence on receptor concentration (57); and chemical cross-linking studies confirm the occurrence of EGF-induced (13, 18) or covalently stabilized (45) receptor dimerization. Most of the studies of the E G F R have ...
Update on the Positive Effects of Light in Humans
... animals have a trace of photosensitive pigment, which has been identified as containing the 9-cis retinal.(9) The source of this isomer is not yet known. There is extensive ongoing research on this and other animal models of the retinoid cycle. The photopigment G protein-coupled receptors (opsin) re ...
... animals have a trace of photosensitive pigment, which has been identified as containing the 9-cis retinal.(9) The source of this isomer is not yet known. There is extensive ongoing research on this and other animal models of the retinoid cycle. The photopigment G protein-coupled receptors (opsin) re ...
One-eyed pinhead regulates cell motility independent of Squint
... functions of oep on cell motility from the more general functions of the zebrafish Nodal pathway on cell specification, we examined the zygotic rather than the maternalzygotic oep mutant. The zygotic pattern of oep transcripts comes up between 4 and 5 hours postfertilization, initially overlapping w ...
... functions of oep on cell motility from the more general functions of the zebrafish Nodal pathway on cell specification, we examined the zygotic rather than the maternalzygotic oep mutant. The zygotic pattern of oep transcripts comes up between 4 and 5 hours postfertilization, initially overlapping w ...
Mechanisms of cell positioning during C. elegans gastrulation
... described above. Analysis of the direction of movement depended on whether MSxx and P4 moved in the same plane or not. In all cases, MSxx and P4 always moved towards the Ea/Ep boundary. If both cells moved in the same plane, the movement was either 0° (towards each other) or 180° (away from each oth ...
... described above. Analysis of the direction of movement depended on whether MSxx and P4 moved in the same plane or not. In all cases, MSxx and P4 always moved towards the Ea/Ep boundary. If both cells moved in the same plane, the movement was either 0° (towards each other) or 180° (away from each oth ...
jxb.oxfordjournals.org
... Fig. S1I). qRT-PCR analysis revealed that the transcription levels of the nine AtGRF genes including seven predicated AtGRF gene targets in 35S:miR396a and 35S:miR396b plants were all decreased (Supplementary Fig. S1J), indicating that miR396 negatively regulates the cell proliferation in leaves by ...
... Fig. S1I). qRT-PCR analysis revealed that the transcription levels of the nine AtGRF genes including seven predicated AtGRF gene targets in 35S:miR396a and 35S:miR396b plants were all decreased (Supplementary Fig. S1J), indicating that miR396 negatively regulates the cell proliferation in leaves by ...
A Golgi and tonoplast localized Sacyl transferase is involved in cell
... potential PATs; alignment of these (Hemsley et al., 2005; Batistic, 2012) shows that all contain the DHHC-CRD and most also possess DPG and TTxE motifs of unknown function (SPG and STxE in AtPAT10) (Mitchell et al., 2006). AtPAT10 has 21.6– 32.4% identity with these predicted proteins, with the gre ...
... potential PATs; alignment of these (Hemsley et al., 2005; Batistic, 2012) shows that all contain the DHHC-CRD and most also possess DPG and TTxE motifs of unknown function (SPG and STxE in AtPAT10) (Mitchell et al., 2006). AtPAT10 has 21.6– 32.4% identity with these predicted proteins, with the gre ...
Tying rings for sex
... barrier formed by four bacterial membranes occurs following the formation of thin, tube-like extracellular filaments, the conjugative pili. It remains an open question whether DNA or other macromolecules per se are transported through the pili. For IncP pili, their brittle structure and loose attach ...
... barrier formed by four bacterial membranes occurs following the formation of thin, tube-like extracellular filaments, the conjugative pili. It remains an open question whether DNA or other macromolecules per se are transported through the pili. For IncP pili, their brittle structure and loose attach ...
REVIEWS
... leave the nucleus, or, more likely, they represent species with a specific nuclear function. Studies are underway to identify and characterize this class of nuclear RNA (K. V. Prasanth and D. L. S., unpublished observations). Of particular interest is whether any of these RNAs represent a structural ...
... leave the nucleus, or, more likely, they represent species with a specific nuclear function. Studies are underway to identify and characterize this class of nuclear RNA (K. V. Prasanth and D. L. S., unpublished observations). Of particular interest is whether any of these RNAs represent a structural ...
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... – Voltage required to maintain that current is measured – Measures changes in the membrane poten3al – At I=0 records approximately the true Vm ...
... – Voltage required to maintain that current is measured – Measures changes in the membrane poten3al – At I=0 records approximately the true Vm ...
ER storage diseases: a role for ERGIC
... in N[⌬CH1] cells treated with 1 mM NaBut for 40 hours, and in untreated J[⌬CH1] cells. Measurements were carried out on electron microscope images. 60 and 30 RB were measured in N[⌬CH1] and J[⌬CH1] cells respectively, each from two independent preparations. ...
... in N[⌬CH1] cells treated with 1 mM NaBut for 40 hours, and in untreated J[⌬CH1] cells. Measurements were carried out on electron microscope images. 60 and 30 RB were measured in N[⌬CH1] and J[⌬CH1] cells respectively, each from two independent preparations. ...
How signaling modalities link oogenesis to
... Follicles • Follicles activate from primordials • Grow to increase somatic cell number (hyperplasia) and support germ cell hypertrophy • Acquire gonadotropin sensitivity along with steroidogenic capacity • Acquire ovulation competency for both the mural and cumulus compartments ...
... Follicles • Follicles activate from primordials • Grow to increase somatic cell number (hyperplasia) and support germ cell hypertrophy • Acquire gonadotropin sensitivity along with steroidogenic capacity • Acquire ovulation competency for both the mural and cumulus compartments ...
Plant Cell
... Much of what is understood about mitosis in plants is based on extrapolation from how cell division functions in other systems, such as yeast (Scheres and Benfey, 1999). Identification of new genes that control this multicellular process in plants is hampered by the lack of a system in which cell di ...
... Much of what is understood about mitosis in plants is based on extrapolation from how cell division functions in other systems, such as yeast (Scheres and Benfey, 1999). Identification of new genes that control this multicellular process in plants is hampered by the lack of a system in which cell di ...
Chapter_4_Tissue_Power_Point
... Simple squamous epithelium Simple cuboidal epithelium Simple columnar epithelium (nonciliated and ...
... Simple squamous epithelium Simple cuboidal epithelium Simple columnar epithelium (nonciliated and ...