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Exam #1
Exam #1

... be A-bb, blue would be aaB-, and white would be aabb. Thus the parental cross was AABB x aabb  AaBb F1. F1 x F1 would give you the F2 progeny ratios seen. Additionally, this can NOT be multiple alleles because you begin with pure breeding birds. Even if there were multiple alleles, there would only ...
Chapter Four Part One - K-Dub
Chapter Four Part One - K-Dub

... has more older siblings and has older (wiser? more tired?) parents. ...
DNA - 長庚大學生物醫學系
DNA - 長庚大學生物醫學系

... both genetic material (like DNA) and a biological catalyst (like protein enzymes), and contributed to the RNA world hypothesis, which suggests that RNA may have been important in the evolution of prebiotic self-replicating systems. Also termed catalytic RNA, ribozymes function within the ribosome (a ...
Chapter Four Part One - K-Dub
Chapter Four Part One - K-Dub

... has more older siblings and has older (wiser? more tired?) parents. ...
Horizontal Gene Transfer Horizontal gene transfer
Horizontal Gene Transfer Horizontal gene transfer

... In most cases, the DNA that is transferred from the donor to the recipient consists merely of a copy of the plasmid. However, some types of plasmids can also promote transfer of chromosomal DNA. The first of these to be discovered, and the best known, is the F (fertility) plasmid of E. coli, but sim ...
Part I
Part I

... 4. Can crossover occur between non-homologous chromosomes? a. Cross-Over cannot occur between non-homologous chromosomes. Homologous pairs consist of two chromosomes, one from the mother and one from the father. Each set of chromosomes serves a specific purpose and codes for certain genes. Informati ...
Test Information Sheet HEXA Gene Analysis in Tay
Test Information Sheet HEXA Gene Analysis in Tay

... more variable neurologic findings, including progressive dystonia, spinocerebellar degeneration, motor neuron disease, and in some individuals with the adult onset form, a bipolar form of psychosis.1 The juvenile and adult onset forms differ from each other primarily by the impact of the disease on ...
Angelman Syndrome (AS) and UBE3A (E6-AP)
Angelman Syndrome (AS) and UBE3A (E6-AP)

... drosophila, but transgenes have shown that imprinting switch regions act as silencers in flies  In marsupials methylation on the X chr preferentially inactivates paternal X  Mouse studies are one of the commonest in the literature ...
Slide 1
Slide 1

... factor IX (F.IX) deficiency, caused by a wide range of mutations on the F.IX gene. Hemophilia B in girls is extremely rare and results from different mechanisms, the most common of which is skewed inactivation of the normal X chromosome in heterozygous girls. In some cases, the inactivation process ...
Technology - Farming Ahead
Technology - Farming Ahead

... alledgedly wanted some type of official stamp of approval from the National Food Authority. Like other areas within the food and fibre industry, this event showed how marketing and consumer perceptions ultimately will decide the fate of genetically engineered products. Negative perceptions of geneti ...
Supplementary Methods
Supplementary Methods

... being unchanged. Cells were then grown for 2 more hours and prepared for fluorescent in situ hybridization (see below). ...
DNA and PROTEIN SYNTHESIS
DNA and PROTEIN SYNTHESIS

... mutagen, it is grown in a culture without histidine. • If it survives it has been mutated! ...
DNA Technology
DNA Technology

... • However, that new organism will be an individual • The two cats seen here are clones but have very different colors ...
Genetics of the bacterial cell
Genetics of the bacterial cell

... properties of mutants showed that the effect of a regulatory gene consists in inhibiting the expression of the structural genes, by forming a cytoplasmic product which was called the repressor. In both cases, the induction of synthesis (whether of phage or of enzyme) seemed to result from a similar ...
Genes and Chromosomes
Genes and Chromosomes

... The placement of centromeres relative to the length of the chromosome is different for each chromosome. Centromeres can be in the center of the chromosome (as the name would suggest), or towards an end. Centromeres consist of some defined sequence elements, but are mostly less well defined repetitiv ...
DNA and PROTEIN SYNTHESIS
DNA and PROTEIN SYNTHESIS

... mutagen, it is grown in a culture without histidine. • If it survives it has been mutated! ...
2013-10-31-Class-lecture
2013-10-31-Class-lecture

... EcoK cleaves -AAC(N6)GTCG- if the second A is unmethylated.  McrBC is removed. McrBC cleaves DNA containing methylcytosine on one or both strands.  High transformation efficiency.  Tight control of expression by laclq (overproduction of LacI) allows potentially toxic genes to be cloned. -35 site ...
PGLO Transformation LAB AP LAB 7
PGLO Transformation LAB AP LAB 7

... together in what is known as the arabinose operon.3 These three proteins are dependent on initiation of transcription from a single promoter, PBAD. Transcription of these three genes requires the simultaneous presence of the DNA template (promoter and operon), RNA polymerase, a DNA binding protein c ...
Sex chromosome-to-autosome transposition - David Page Lab
Sex chromosome-to-autosome transposition - David Page Lab

... exception of the rodent lineage (Fig. 1) [1, 6]. However, mouse and rat both contain an intact and actively transcribed autosomal EIF1A retrogene (Figs. 1b and 2b; Additional files 1, 2 and 3). Of the species retaining Y-linked copies of this gene, we found a retrotransposed copy in cattle, but it h ...
Document
Document

... Utilizing a diverse scientific community to study intensive and important topics using comparative genomics with the Solanaceae as it provides a unique set of crop species that is important to: -Investigate plant response to abiotic stress, biotic stress and development (done) ...
sample report - Integrated Genetics
sample report - Integrated Genetics

... mutations in the FMR1 gene. Reported CGG repeat sizes may vary as follows: +/- one for repeats less than 60, and +/- two to four for repeats in the 60 - 120 range respectively. For repeats greater than 120, the accuracy is +/- 10%. Limitations: Next generation sequence analysis does not detect germl ...
Genetics of the bacterial cell
Genetics of the bacterial cell

... properties of mutants showed that the effect of a regulatory gene consists in inhibiting the expression of the structural genes, by forming a cytoplasmic product which was called the repressor. In both cases, the induction of synthesis (whether of phage or of enzyme) seemed to result from a similar ...
Zoo/Bot 3333
Zoo/Bot 3333

... acting on flower color. A and B are required for color. What would be the predicted ratio of colored to colorless among the progeny of an F1 cross, where the parentals were two colorless plants, one homozygous for A and the other homozygous for B? a) 9:3:3:1; b) 9:7; c) 9:3:4; d) 12: 3: 1; e) none o ...
Exercise III - GEP Community Server
Exercise III - GEP Community Server

... the generation by breeders of semi-dwarf varieties of wheat, maize and rice that did not grow as tall as their predecessors, allowing them to divert more resources into building seeds while diminishing stalk breaking due to long stems and heavy seed loads. However, while highly desirable also for ot ...
Document
Document

... • In practice it is impossible to achieve absolute independence of replicates. For example, the same researcher often does all the replicates, but the results may differ in the hands of another person. • But it is very important to reduce dependency between replicates to a minimum. For example, it i ...
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Site-specific recombinase technology



Nearly every human gene has a counterpart in the mouse (regardless of the fact that a minor set of orthologues had to follow species specific selection routes). This made the mouse the major model for elucidating the ways in which our genetic material encodes information. In the late 1980s gene targeting in murine embryonic stem (ES-)cells enabled the transmission of mutations into the mouse germ line and emerged as a novel option to study the genetic basis of regulatory networks as they exist in the genome. Still, classical gene targeting proved to be limited in several ways as gene functions became irreversibly destroyed by the marker gene that had to be introduced for selecting recombinant ES cells. These early steps led to animals in which the mutation was present in all cells of the body from the beginning leading to complex phenotypes and/or early lethality. There was a clear need for methods to restrict these mutations to specific points in development and specific cell types. This dream became reality when groups in the USA were able to introduce bacteriophage and yeast-derived site-specific recombination (SSR-) systems into mammalian cells as well as into the mouse
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