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presentation (spanish ppt format, 4.7 MB)
presentation (spanish ppt format, 4.7 MB)

... Each siRNA is unwound into two single-stranded (ss) ssRNAs (passenger strand and the guide strand). The passenger strand is degraded (red), and the guide strand (blue) is incorporated into the RNA-induced silencing complex (RISC). The most well-studied outcome is post-transcriptional gene silencing, ...
Ch. 5: Presentation Slides
Ch. 5: Presentation Slides

... DNA Sequence: convention 5’ to 3’end, one strand (because other strand is complementary and therefore known also) ...
Tissue DNA extraction and PCR determinations
Tissue DNA extraction and PCR determinations

... Tissue DNA extraction and PCR determinations DNA extraction Genomic DNA was extracted from 50 - 100 mg of maternal and foetal tissue samples and 200 µL of foetal fluids using the commercial kit Maxwell® 16 Mouse Tail DNA Purification Kit, developed for the automated Maxwell® 16 System (Promega, Wis ...
Concepts in Biology, First Edition Sylvia Mader
Concepts in Biology, First Edition Sylvia Mader

... pass on traits  The law of segregation states:  Each individual has two factors for each trait  The factors segregate (separate) during the formation of the gametes  Each gamete contains only one factor from each pair of factors  Fertilization gives each new individual two factors for ...
Chapter 11: DNA and the Language of Life - Rebecca Waggett
Chapter 11: DNA and the Language of Life - Rebecca Waggett

... DNA vs. RNA DNA ...
Here
Here

... coordinated effort of a set of genes. Such activity is often carried out through the organization of the genome into regulatory modules. Modules are sets of co-regulated genes that share a common function. The identification of modules, their regulators, and the conditions under which regulation occ ...
prism
prism

... genes defined by preassigned functional annotation. Pairs of epistatically interacting genes were more likely to share the same annotation (21%). The interactions between genes from 2 different annotations tend to be either exclusively buffering or exclusively aggravating! This property, which we ca ...
Non-coding RNA | Principles of Biology from Nature Education
Non-coding RNA | Principles of Biology from Nature Education

... This organism has proven invaluable for gene function studies, especially during development. An embryo at hatching has only 558 cells but contains all the major types of differentiated tissues found in other animals. microRNAs were first identified from studies of C. elegans development. NASA. miRN ...
ACT - Operon -S - saddlespace.org
ACT - Operon -S - saddlespace.org

... smaller molecules in the environment? Justify your answer with specific details from Model 1 or Model 2. ...
Bioreactors and transgenic animals
Bioreactors and transgenic animals

... gives us the hypothetical ability to do things never contemplated before". Team of 20 top scientists, led by the H. Smith has constructed a synthetic chromosome based on the bacterium Mycoplasma genitalium, that is 381 genes long and contains 580,000 base pairs of genetic code. The synthetically rec ...
Control of Gene Expression in Prokaryotes
Control of Gene Expression in Prokaryotes

... Control of Gene Expression in Prokaryotes How do prokaryotes use operons to control gene expression? ...
Genetics of Beef Cattle: Moving to the genomics era Matt Spangler
Genetics of Beef Cattle: Moving to the genomics era Matt Spangler

... In contrast to the thought process of DNA marker panel results being a separate and disjoined piece of information, these test results should be thought of as a potentially useful indicator that is correlated to the trait of interest. As such, the MBV can be included in the National Cattle Evaluatio ...
Exam 1
Exam 1

... (i) Phage grown on S. enteritidis is restricted by S. typhimurium and therefore it must not be modified for the S. typhimurium R-M system. (You cannot tell if S. enteritidis has an R-M system itself, only that it does not have the S. typhimurium systems.) (ii) All three R-M systems in S. typhimurium ...
An informatics approach to analyzing the incidentalome
An informatics approach to analyzing the incidentalome

... clinical database due to the data querying and predictive algorithms. ...
Ingram 1957
Ingram 1957

... molecule, an alteration le~ding to a protein which is by all criteria still a h remoglobin. It is now clear that, p er half-molecule of hremoglobin, this change consists in a replacement of only one of nearly 300 am~no-acids, namely, gluta~ic acid, by another, v a lme-a very small change mdeed. Diff ...
The possibilities of practical application of transgenic mammalian
The possibilities of practical application of transgenic mammalian

... recently used in the prophylactic treatment of patients with congenital antithrombin deficiency. Systems for efficient targeted genetic transformations (knock-out; locus-specific mutagenesis) by homologous recombination in somatic nuclear donor cells are being developed and the adaptation of sophist ...
X chromosome - Fort Bend ISD
X chromosome - Fort Bend ISD

... • Genetic disorder where the body produces abnormally thick mucus in the lungs and intestines making respiration and digestion difficult • caused by a mutation in a gene. The product of this gene is a chloride ion channel important in creating sweat, digestive juices and mucus. • One in four babies ...
PowerPoint - 埼玉医科大学総合医療センター 内分泌・糖尿病内科
PowerPoint - 埼玉医科大学総合医療センター 内分泌・糖尿病内科

... PDC Pyruvate dehydrogenase complex PDK4 Pyruvate dehydrogenase kinase 4 siRNA Small interfering RNA TCF7L2 Transcription factor 7-like 2 TSS Transcriptional start site WNT Morphogenic wingless-type MMTV integration site family ...
Supplementary Methods.
Supplementary Methods.

... enhancer activity (which yielded TTNNTTT). Because the latter two comparisons are between sets of sequences for which we have alignments with mouse and fugu, in those ...
Unit 4 Genetics - Jamestown Public Schools
Unit 4 Genetics - Jamestown Public Schools

... Phases of Meiosis Meiosis - process of reduction division, where the # of chromosomes per cell is cut in 1/2, through the separation of homologous chromosomes in a diploid ...


... it “is the first specific information we have on the molecular events that accompany a developmental switch between alternative pathways in insects in general. We now have a proven technique that can be used to delve into the details of what goes on at the molecular level when developmental pathways ...
Lecture 6 Quiz
Lecture 6 Quiz

... 3.The following functions are all supposed to count how many times a certain base (represented as a character variable in Python) appears in a dna sequence (represented as a string variable in Python): def count1(dna, base): ...
Gene Therapy and Genetic Engineering: Frankenstein is Still a Myth
Gene Therapy and Genetic Engineering: Frankenstein is Still a Myth

... a nucleus. Within the nucleus are located 46 chromosomes which can be grouped into 23 homologous pairs. These pairs are called homologs because the members of the pair appear to be identical under a microscope. Each homologous chromosome consists of the same linear sequence of genes, the particulate ...
Mining Gene Regulatory Networks and Microarray Data: The
Mining Gene Regulatory Networks and Microarray Data: The

... knowledge integration, and new tools to analyze and mine clinicogenomic data at all levels (gene, protein, molecular pathway, tissue, individual and population). Current post-genomics bioinformatics research seeks for methods that not only combine the information from dispersed and heterogeneous dat ...
1 A. You have the following piece of genomic DNA with the two
1 A. You have the following piece of genomic DNA with the two

... 12. Name another alternative strategy that can be used to confirm this conclusion? 13. Describe the steps you would use to perform this second alternative strategy? 14. Taking into account the conclusions you have made up to this point, examine the provided sequence. What can you conclude about the ...
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Site-specific recombinase technology



Nearly every human gene has a counterpart in the mouse (regardless of the fact that a minor set of orthologues had to follow species specific selection routes). This made the mouse the major model for elucidating the ways in which our genetic material encodes information. In the late 1980s gene targeting in murine embryonic stem (ES-)cells enabled the transmission of mutations into the mouse germ line and emerged as a novel option to study the genetic basis of regulatory networks as they exist in the genome. Still, classical gene targeting proved to be limited in several ways as gene functions became irreversibly destroyed by the marker gene that had to be introduced for selecting recombinant ES cells. These early steps led to animals in which the mutation was present in all cells of the body from the beginning leading to complex phenotypes and/or early lethality. There was a clear need for methods to restrict these mutations to specific points in development and specific cell types. This dream became reality when groups in the USA were able to introduce bacteriophage and yeast-derived site-specific recombination (SSR-) systems into mammalian cells as well as into the mouse
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