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Infectious laryngotracheitis (ILT) is an acute and highly contagious
Infectious laryngotracheitis (ILT) is an acute and highly contagious

... of wild-type and vaccine strains have been analysed, but none was from Europe. The aim of this study was to determine and analyse the complete genome sequences of five Italian ILTV strains. Sequences were also compared to reveal the similarity of strains across time and to discriminate between wild- ...
Deciphering Pathogens: Blueprints for New Medical Tools
Deciphering Pathogens: Blueprints for New Medical Tools

... which is caused by a single-celled parasite spread to people by mosquitoes, kills up to 2.7 million people annually, most of them African children. Between 400 million and 900 million acute malaria cases occur each year in African children alone. HIV/AIDS first emerged in the early 1980s and has sin ...
This is going to be a long journey, but it is crucial
This is going to be a long journey, but it is crucial

... grading anything that cannot be easily and quickly read so start perfect your handwriting. Please SCAN documents properly and upload them to Archie. Avoid taking photographs of or uploading dark, washed out, side ways, or upside down homework. Please use the scanner in the school’s media lab if one ...
Genetic Polymorphism and Variability of Chemical Carcinogenesis
Genetic Polymorphism and Variability of Chemical Carcinogenesis

... letters, while isoforms are marked by Arabic numerals. For example, CYP2D6 means cytochrome P450, family 2, subfamily D, polypeptide 6. CYP genes of all mammalian species are arranged into 18 families. The number of subfamilies in each family depends on the species. Each CYP isoform has its own set ...
Recombinant DNA and Biotechnology
Recombinant DNA and Biotechnology

... antibiotic resistance gene (e.g., ampicilin resistance) on a plasmid mutagenesis - Site-directed • For viral vectors, use of “infected” phenotype. • Use of “selectable markers” to detect either insertion into the vector or incorporation into the host. Some of these are a type of reporter gene—a gene ...
CHAPTER 15 Gene Mapping in Eukaryotes
CHAPTER 15 Gene Mapping in Eukaryotes

... 1. With autosomal recessive alleles, when a double heterozygote is testcrossed, four phenotypic classes are expected. If the genes are linked, the two parental phenotypes will be about equally frequent and more abundant than the two recombinant phenotypes(Figure 15.4). 2. Mapping of genes with other ...
Tumor-Suppressor Genes
Tumor-Suppressor Genes

... • In electron micrographs, unfolded chromatin has the appearance of beads on a string • Each “bead” is a nucleosome, the basic unit of ...
Document
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... synthesis.2 Identifying and incorporating specific mutations in specific viral genes that would allow it to go undetected by current molecular tests depends on the size and complexity of the viral genome. Larger, more complex viruses are much more difficult to manipulate and maintain their ability ...
l(1)sc
l(1)sc

... • bHLH transcription factors • Control neuroblast formation and cell fate in the lateral CNS ...
When Is Hub Gene Selection Better than
When Is Hub Gene Selection Better than

... When does hub gene selection lead to more meaningful gene lists than a standard statistical analysis based on significance testing? • Here we address this question for the special case when multiple data sets are available. • This is of great practical importance since for many research questions m ...
Yeast genetics to investigate the function of core pre
Yeast genetics to investigate the function of core pre

... 3.2.2 Construction of double knockout strains The construction of a double knockout strain with a complementing URA3 plasmid with the two wild-type genes can be carried out by two different methods. The first method involves mating two haploid knockout strains of opposite mating types where each kno ...
Biology end of the year material review
Biology end of the year material review

... 33. If a homozygous tall, homozygous round seeded plant is crossed with a heterozygous tall, heterozygous round seeded plant, what percent of the offspring would be homozygous tall, homozygous round? 34. The gene for color vision (C) is dominant to the gene for color blindness (c) and is located on ...
insertion mutation
insertion mutation

... • Children born with this disorder cannot make an enzyme that is critical in breaking down fat and toxic substances in the brain. • The disease is terminal. Most will die before age ...
18440: Probability and Random variables Quiz 1, Version 2
18440: Probability and Random variables Quiz 1, Version 2

... 18440: Probability and Random variables Quiz 1, Version 2 Wednesday, October 22, 2014 ...
Genetics notes, long version
Genetics notes, long version

... Genes are short pieces of DNA that decide what form of a genetic trait you will have. The average size of a gene is about 5000 base pairs, but they can be much longer. It is the order of the A’s, T’s, C’s and G’s in the gene that decide how it will work. COPYING DNA ...
Abstract - UWL faculty websites
Abstract - UWL faculty websites

... contains cell division proteins that are similar to those in human cells. One commonly studied yeast cell growth protein is Cdc7. This protein kinase is required for initiating DNA replication (S phase) during the mitotic cell cycle, although it is not understood how Cdc7 protein controls this initi ...
ppt
ppt

... the sample sizes here are not too small ( two samples of 8 each gives 16 terms in the difference of the means), approximate normality is not an unreasonable expectation for the null marginal distribution. Converting ranked t’s into a normal qq-plot is a great way to see the extremes: they are the on ...
PDF
PDF

... in vivo by assembly lines of many kinetically linked enzymes (12). It is for this reason that aneuploidy can change cellular phenotypes much better than ...
Genetics Test - WOHS Biology
Genetics Test - WOHS Biology

... -Copy or rewrite the work problems and practice them. -Use your original paper as the key. -See me if you need to verify the answers. -Need to also know the vocabulary and how to use the word. - Chapter 10 Assessment p278-279 and Chapter 12 Assessment p334-335 Meiosis, Section 10.2 pages p263-273 (S ...
lecture7
lecture7

... Unit of Study In Population Genetics ” A population in the genetic sense, is not just a group of individuals but a breeding group; and the genetics of a population is concerned not only with the genetic constitution of the individuals but also with the transmission of the genes from one generation t ...
High-throughput cloning of eukaryotic open reading frames (ORFs
High-throughput cloning of eukaryotic open reading frames (ORFs

... The Center for Eukaryotic Structural Genomics (CESG) was founded as a collaborative effort to develop technologies for the rapid and economic determination of protein three-dimensional structures. The initial focus was on the genome of the model plant Arabidopsis thaliana. Open reading frames (ORFs) ...
P10
P10

... • Give examples of some exceptions to this rule, and describe how the alteration in the amino acid sequence are generated. – exceptions to this rule can arise, for example, from splice site mutations that lead to missplicing of an exon. The exon may be excluded from the mRNA, generating either an in ...
Welcome AP Super
Welcome AP Super

... – This disorder is the most common genetic disorder within the black population. Other populations can get it too. It is not exclusive. – It affects 1 in 400 births. – The 6th Amino Acid is changed (Glutein  Valine) in the PRIMARY sequence of one of the proteins needed to make red blood cells. (The ...
Universal donor cells – a revolution against rejection
Universal donor cells – a revolution against rejection

... whereby two similar (homologous) DNA molecules exchange genetic material without introducing any cuts or damage to the DNA itself. The rAAV can be designed to insert any genetic material to a very precise location in the genome. During UDC manufacturing, the HLA-E transgene described above is encode ...
Genetic Testing
Genetic Testing

... disease. It is therefore possible to test a blood sample for the presence or absence of this gene mutation. For many conditions - for example, diabetes - there may be any one of hundreds or even thousands of different possible mutations in a particular gene. This means genetic testing for those cond ...
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Site-specific recombinase technology



Nearly every human gene has a counterpart in the mouse (regardless of the fact that a minor set of orthologues had to follow species specific selection routes). This made the mouse the major model for elucidating the ways in which our genetic material encodes information. In the late 1980s gene targeting in murine embryonic stem (ES-)cells enabled the transmission of mutations into the mouse germ line and emerged as a novel option to study the genetic basis of regulatory networks as they exist in the genome. Still, classical gene targeting proved to be limited in several ways as gene functions became irreversibly destroyed by the marker gene that had to be introduced for selecting recombinant ES cells. These early steps led to animals in which the mutation was present in all cells of the body from the beginning leading to complex phenotypes and/or early lethality. There was a clear need for methods to restrict these mutations to specific points in development and specific cell types. This dream became reality when groups in the USA were able to introduce bacteriophage and yeast-derived site-specific recombination (SSR-) systems into mammalian cells as well as into the mouse
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