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Supplementary Figure Legends (doc 38K)
Supplementary Figure Legends (doc 38K)

... ChIP-seq data. Regions amplified by ChIP-PCR are indicated in each gene. Fig. S12. Ectopic expression of Sp1 promotes the binding of Sp1 to p53 target genes and p53-mediated pro-apoptotic transcriptional repression in MCF-7 cells upon nutlin treatment. (A) Binding of Sp1 to selected genes (described ...
reviews
reviews

... The recent results derived from evolutionary, developmental and genomic studies in various organisms highlight the key roles of gene and phenotypic multifunctionality during organismal evolution20,85. Genetic evidence of gene multifunctionality has a long history and was first described in maize86 a ...
Chapter 9 Genetics
Chapter 9 Genetics

... B. 9.12 Many genes have more than two alleles in the population 1. Although each individual carries, at most, two different alleles for a particular gene, in cases of multiple alleles, more than two possible alleles exist in a population. 2. Human ABO blood group phenotypes involve three alleles for ...
Research lifts early vigour and yields in wheat
Research lifts early vigour and yields in wheat

... Transpiration efficiency Crops with high transpiration efficiency, like the recently released Drysdale wheat variety, yield more with less water. While the method used to measure transpiration efficiency in Drysdale is effective, it is also expensive and unfortunately cannot be automated. To overcom ...
practice!
practice!

... a. They are temporally isolated from one another. b. They are geographically isolated from one another. c. They are members of the same species. d. They have identical genes. 2. Which statement about gene pools is typically true? a. They contain two or more alleles for each gene. b. They contain onl ...
Genetic and Molecular Abnormalities in Tumors of the Bone and Soft
Genetic and Molecular Abnormalities in Tumors of the Bone and Soft

... A recently described t(12;15)(p13;q25) rearrangement in CFS fuses the helix-loop-helix protein dimerization domain of the ETV6 gene (also known as TEL) in 12p13 with the protein tyrosine kinase domain of NTRK3, neurotrophin-3 receptor gene (also known as TRKC) in 15q25. ETV6-NTRK3 chimeric transcrip ...
Genetic diversity and phylogenetic classification of viral hemorrhagic
Genetic diversity and phylogenetic classification of viral hemorrhagic

... septicemia virus (VHSV) and to gain insight into the molecular epidemiology of this fish rhabdovirus. The sequences of the nonstructural (NV) protein and the transmembrane (G) protein of sequential North American and European isolates of VHSV were determined and used to compute phylogenetic trees. A ...
Final Genetic Problems for IBO 2014 PART I In Drosophila
Final Genetic Problems for IBO 2014 PART I In Drosophila

... 13. Life at high altitudes requires special adaptations of the body. In a genome wide study Yi et al. identified the Endothelial PAS domain-containing protein 1 (EPAS1) as a promising candidate gene for such adaptations. For this study, Yi et al. only used genetic data. Indicate whether the followin ...
5 The Genetics of Bacteria and Their Viruses
5 The Genetics of Bacteria and Their Viruses

... during which the transfer occurred. Therefore, a population containing several different Hfr strains will appear to have an almost random transfer of host genes. This is similar to generalized transduction, in which the viral protein coat forms around a specific amount of DNA rather than specific ge ...
PDF - WashU Epigenome Browser
PDF - WashU Epigenome Browser

... Learn more about the supported numerical track formats bedGraph (http: //wiki.wubrowse.org/bedgraph) and bigWig (http://wiki.wubrowse.org/bigwig). ...
supplement 3 - Springer Static Content Server
supplement 3 - Springer Static Content Server

... When the tissue specific genes (only colored genes in Figure 1) were plotted onto this new component space (see Figure 2), it was shown that the second and third components, PC2 and PC3, beautifully recognize the 6 classes of tissue specific genes. The first component, which is associated with the e ...
cookie-aseSHO
cookie-aseSHO

Introduction to Molecular Pathology
Introduction to Molecular Pathology

... mRNA degradation RNA interference or silencing  miRNA and siRNA ...
Table S3 - BioMed Central
Table S3 - BioMed Central

... is 80% or more amino acid sequence similarity between homologs in different species. Through this index the user can additionally increase the interspecies similarity threshold, e.g. a search for >95 on this index will return only the homology-inferred relations based on more than 95% similarity. Th ...
Genotype to phenotype: lessons from model organisms
Genotype to phenotype: lessons from model organisms

... number of transient protein interactions; and enrichment for protein domains that bind to linear motifs. These features suggest the hypothesis that one of the causes of dosage sensitivity is mass-action-driven promiscuous molecular interactions41. However, it is likely that promiscuous (‘off-target’ ...
Recombinant DNA Technology
Recombinant DNA Technology

... (melted), it will pair up with another DNA (or RNA) with the complementary sequence. If one of the DNA molecules is labeled, you can detect the hybridization. ...
Slide 1
Slide 1

... apoptosis. When they are activated by signals, the important proteins in cell will be degenerated to cause the cell turn to apoptosis irreversibly. The caspases keep the features as the follows: ① The enzyme activity depends on the nuclear affinity of cysteine residue; ② The substrate is always cut ...
Microevolution ppt
Microevolution ppt

... Once a mutation occurs, nearly all phenotypic variations will result from shuffling of existing alleles ...
One vitellogenin gene in an ocean of many: The molecular ecology
One vitellogenin gene in an ocean of many: The molecular ecology

... matches within Alveolata WGS and TSAs. Blast hits of up to 96% identity were found within TSAs for Symbiodinium species, and we suggest that the aqpH1-5 orthologs are likely derived from zooanthellate endosymbiotic dinoflagellates. Similarly, the divergent starlet sea anemone sequence (EDO28361) enc ...
early RNs, crossing over initiates, then synapsis begins Chiasmata
early RNs, crossing over initiates, then synapsis begins Chiasmata

... •Ac transposable element is autonomous: it encodes the gene for the transposase enzyme that allows it to jump. Some maize lines have active Ac. •Ds element is non -autonomous: it is a derivative of Ac but its transposase gene does not function. However, when Ac is present in same genome, Ds can jump ...
On intrapersonal reciprocity
On intrapersonal reciprocity

... Silverman, 2001; Haig, 2002)? The simple answer is little: genes of both parental origins would favor withholding the benefit from Maddy if B < 2C and both sets of genes would favor conferring the benefit if this directly benefited Bob (C < 0). Specific prediction of outcomes within the zone of conf ...
Color Genetics of the Dwarf Hotot
Color Genetics of the Dwarf Hotot

... loci are on separate chromosomes. This means they can each have an equal chance of being passed on as only one chromosome from each parent is passed on; this is why there is one copy of each gene from each parent. This assumption allows us to determine probabilities like we did with the Punnett Squa ...
2 Mendelismo
2 Mendelismo

... to the first recombinant DNA experiments in 1973, which touched off another revolution in genetic research. Walter Gilbert (b. 1932) and Frederick Sanger (b. 1918) developed methods for sequencing DNA in 1977. The polymerase chain reaction, a technique for quickly amplifying tiny amounts of DNA, was ...
WORKSHEET 6.4-6.6 Section 6.4 – Traits, Genes and Alleles 1
WORKSHEET 6.4-6.6 Section 6.4 – Traits, Genes and Alleles 1

... Yes. The chromosomes carrying those genes will line up randomly and separate randomly during meiosis. 8. If genes A and B are located at opposite ends on the same chromosome, are they likely to follow Mendel’s law of independent assortment? Explain. Yes. The genes will be far enough from each other ...
T. caerulescens
T. caerulescens

... different way and had to be altered in order to understand the significance • The data on excel was then normalized in order to fit the GenMAPP protocol • GenMAPP is used to visualize gene expression – Helps to group genes together and find its functional expression for the subject ...
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Site-specific recombinase technology



Nearly every human gene has a counterpart in the mouse (regardless of the fact that a minor set of orthologues had to follow species specific selection routes). This made the mouse the major model for elucidating the ways in which our genetic material encodes information. In the late 1980s gene targeting in murine embryonic stem (ES-)cells enabled the transmission of mutations into the mouse germ line and emerged as a novel option to study the genetic basis of regulatory networks as they exist in the genome. Still, classical gene targeting proved to be limited in several ways as gene functions became irreversibly destroyed by the marker gene that had to be introduced for selecting recombinant ES cells. These early steps led to animals in which the mutation was present in all cells of the body from the beginning leading to complex phenotypes and/or early lethality. There was a clear need for methods to restrict these mutations to specific points in development and specific cell types. This dream became reality when groups in the USA were able to introduce bacteriophage and yeast-derived site-specific recombination (SSR-) systems into mammalian cells as well as into the mouse
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