• Study Resource
  • Explore Categories
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
BREEDING BUNNIES State Standard 7.a. Students know why
BREEDING BUNNIES State Standard 7.a. Students know why

... form next to “Generation 1”. For instance, if you draw one red and one white bean, place a mark in the chart under “Number of Ff individuals.” Continue drawing pairs of beans and recording the results in your chart until all beans have been selected and sorted. Place the “rabbits” into the appropria ...
Coat Color Genetics
Coat Color Genetics

... chromosomes. – Each chromosome was initially created through fertilization, where genetic information from the sire (father) was united with genetic information from the dam (mother). – Loci and Alleles are also found on chromosomes. ...
Linear Regression (1/1/17)
Linear Regression (1/1/17)

... distribution, which would eliminate the differences in population-specific expression, but might be too large a correction and eliminate some of the eQTL signals as well. In this paper, they used r2 values to quantify possible associations. They identified how much of the total variance of each gene ...
Document
Document

... VHL gene mu tations by direct sequencing were performed in 20 tumors. TFE3 protein overexpression, which correlates with the presence of a TFE3 gene fusion, was assessed by immunohistochemistry. Applying the new WHO classification for renal cell carcinoma, there we re 6 clear cell ( 15 %), 9 papilla ...
teacher`s notes: survival in wild turkeys
teacher`s notes: survival in wild turkeys

... genotype for the first team's scenario. Using extra tiles, a random gene for each of the five gene loci is selected from both turkeys. This results in a recombined genotype for a turkey offspring. Each team checks to see if its offspring is better-adapted to their scenario than their original turkey ...
Meiosis II
Meiosis II

... A map unit is an arbitrary unit of measure used to describe the relative distances between linked genes. The number of map units between two genes or between a gene and the centromere is equal to the percentage of recombinants. Customary units cannot be used because we cannot directly visualize gene ...
Rare Genetic Diseases with Human Lean and/or Starvation
Rare Genetic Diseases with Human Lean and/or Starvation

... genetic causes of two of these diseases, abetalipoproteinemia and hypobetalipoproteinemia, have been elucidated. In abetalipoproteinemia a frameshift mutation in the microsomal triglyceride transfer protein (MTP) gene was described that results in complete absence of the MTP and its activity. This m ...
A phenotype-based screen for embryonic lethal mutations in the mouse
A phenotype-based screen for embryonic lethal mutations in the mouse

... (2). To gain a deeper understanding of the events that program mammalian embryogenesis, it will be necessary to identify and characterize the function of additional genes required for mouse development. Recent advances in positional cloning have made the molecular identification of mammalian genes d ...
Understanding public and private genetic testing for cancer risk
Understanding public and private genetic testing for cancer risk

... Gene fault found - Your cancer risk will depend on which gene is involved. You will be given advice about cancer screening and/or prevention and what this means for your family. No gene fault found - Testing cannot rule out a gene fault unless the cause of cancer in your family is already known. You ...
Inquiry into Life Twelfth Edition
Inquiry into Life Twelfth Edition

... Antitermination • Antitermination is a type of transcriptional switch • A gene product serves as antiterminator that permits RNA polymerase to ignore terminators at the end of the immediate early genes • Same promoters are used for both immediate early and delayed early transcription • Late genes a ...
Touring Ensembl: A practical guide to genome browsing Open Access
Touring Ensembl: A practical guide to genome browsing Open Access

Supporting Online Material
Supporting Online Material

... animals having undergone puparium formation in certain time points are shown in Figure 3B. To stage the silenced and non-silenced pupae, wandering larvae were collected from the wall of the tubes. GFP-expression was determined on living animals using a Leica DMLS fluorescence microscope. Silenced an ...
Genetics
Genetics

... This can be seen in human diseases such as cystic fibrosis or sickle cell anemia. In these diseases, multiple symptoms can be traced back to one defective allele. ...
69 Evidence from DNA
69 Evidence from DNA

... for sure? DNA typing can be used to check for exact DNA matches. This is sometimes called DNA fingerprinting because it gives a unique result that helps identify people, but it is actually very different from regular fingerprinting. Since DNA fingerprints of relatives are much more alike than those ...
Copy Number and Gene Expression Integration in Partek
Copy Number and Gene Expression Integration in Partek

... data associated with this tutorial from within Partek at Help > On-line Tutorials. After the file has been unzipped, simply open the Partek project by selecting File > Open Project and browse to the cifola.ppj file within the unzipped data. A collection of several files containing both gene expressi ...
Introductory Bacterial Conjugation Kit
Introductory Bacterial Conjugation Kit

... resistance to antibiotics enables the new recombinant bacterial cell to express resistance to an antibiotic to which it was formerly sensitive. This transfer is also considered a type of genetic recombination. While bacterial chromosomes normally carry all the genes necessary for growth and reproduc ...
Doubling Down on Genomes: Polyploidy and Crop Plants
Doubling Down on Genomes: Polyploidy and Crop Plants

... wheat, reduction in genome size may occur relatively rapidly, after only a few generations in some synthetic lines (Eilam et al., 2008, 2010), serving to exemplify the “revolutionary” (Feldman and Levy, 2009) pace of genomic alterations that impact allopolyploid genomes. What ...
qRT-PCR Primer Design Using IDT Primer Quest Dr. Ray Enke Bio
qRT-PCR Primer Design Using IDT Primer Quest Dr. Ray Enke Bio

... You now have 2 ApE files that we will use to ID the exon/intron junctions. This is important because the primers we design will span one of these junctions so that only cDNAs and not contaminating genomic DNA will be amplified by our primer sets. II. Annotating mRNA exon junctions in ApE Sequence Ed ...
The Genetics of Parkinson A version for the interested lay
The Genetics of Parkinson A version for the interested lay

... PARK 2 is autosomal recessive.  If both genes are affected, the individual will develop the disease at a young age, usually before age 40.  If only one gene is affected, there is the possibility – still unclear – that there is a PREDISPOSITION to develop the disease. ...
HMG 9_8.book(ddd138.fm)
HMG 9_8.book(ddd138.fm)

... transient episodes of cholestasis but do not develop hepatic failure (12). The FIC1 gene encodes a P-type ATPase, which is believed to play a role in the enterohepatic circulation of bile acids. PFIC2 is caused by mutations in the bile salt export pump (BSEP) gene (13) (also called ABCB11). The clin ...
Age - xenia.sote.hu!
Age - xenia.sote.hu!

... This adaptation includes an ability to recognise the abnormal substances which we come into contact with in daily life. Hence if this capacity is reduced then the organism becomes ever greatly prone to disease and damage. ...
Monohybrid crosses in humans
Monohybrid crosses in humans

... part of the eyes and a blue layer at the back of the iris shows through, resulting in blue eyes. A dominant allele of this gene, P , causes pigment to be deposited in the front layer of the iris, thus masking the blue to varying degrees. Other genes determine the exact nature and density of this pig ...
Epigenetic Regulation of the Glucocorticoid receptor in human brain
Epigenetic Regulation of the Glucocorticoid receptor in human brain

... cellular phenotype caused by mechanisms other than changes in the underlying DNA nucleotide sequence. DNA methylation and histone deacetylation are two processes which can cause these heritable changes. ...
Supplementary Data
Supplementary Data

... Primers in the coding regions of the GAPDH gene were designed in intron sequences or with one primer in the intron and the reverse primer in exon sequences to prevent amplification of pseudogenes. Sequence positions are given relative to the transcription start site. PCR products were resolved on 6% ...
Inferring gene-to-phenotype and gene-to
Inferring gene-to-phenotype and gene-to

... provides a vital time-savings relative to manual annotation by curators. Keywords: Phenotype, Genotype, Disease, Mouse, Annotation ...
< 1 ... 440 441 442 443 444 445 446 447 448 ... 1288 >

Site-specific recombinase technology



Nearly every human gene has a counterpart in the mouse (regardless of the fact that a minor set of orthologues had to follow species specific selection routes). This made the mouse the major model for elucidating the ways in which our genetic material encodes information. In the late 1980s gene targeting in murine embryonic stem (ES-)cells enabled the transmission of mutations into the mouse germ line and emerged as a novel option to study the genetic basis of regulatory networks as they exist in the genome. Still, classical gene targeting proved to be limited in several ways as gene functions became irreversibly destroyed by the marker gene that had to be introduced for selecting recombinant ES cells. These early steps led to animals in which the mutation was present in all cells of the body from the beginning leading to complex phenotypes and/or early lethality. There was a clear need for methods to restrict these mutations to specific points in development and specific cell types. This dream became reality when groups in the USA were able to introduce bacteriophage and yeast-derived site-specific recombination (SSR-) systems into mammalian cells as well as into the mouse
  • studyres.com © 2026
  • DMCA
  • Privacy
  • Terms
  • Report