The Schistosoma gene discovery program: state of the art
... great conservation of some genes in the various species, information obtained in one organism can be of relevance to the other. For both S. mansoni and S. japonicum, the Network decided not to adopt a reference strain as there is no evidence for gross chromosomal rearrangements among strains [16,17] ...
... great conservation of some genes in the various species, information obtained in one organism can be of relevance to the other. For both S. mansoni and S. japonicum, the Network decided not to adopt a reference strain as there is no evidence for gross chromosomal rearrangements among strains [16,17] ...
splice sites at the termini generating a novel intron from a dSpm
... of a given dSpm have been isolated, which differ in the somatic and germinal excision frequencies in the presence of En/Spm (for review, see Fedoroff, 1989). McClintock (1968) has termed these derivatives 'changes in state'. Molecular analysis of such 'states' of a dSpm element has revealed that the ...
... of a given dSpm have been isolated, which differ in the somatic and germinal excision frequencies in the presence of En/Spm (for review, see Fedoroff, 1989). McClintock (1968) has termed these derivatives 'changes in state'. Molecular analysis of such 'states' of a dSpm element has revealed that the ...
Non Nuclear Inheritance
... Schwartz and Vissing from Copenhagen have discovered that one of their patients inherited the majority of his mitochondria from his father. Mitochondria in the sperm from the father were presumed to be destroyed immediately after fertilisation, leaving behind only those from the mother. The two rese ...
... Schwartz and Vissing from Copenhagen have discovered that one of their patients inherited the majority of his mitochondria from his father. Mitochondria in the sperm from the father were presumed to be destroyed immediately after fertilisation, leaving behind only those from the mother. The two rese ...
Biology 540/CAMB 541
... The genetic basis for organismal diversity is receiving increasing attention, aided by the development of new experimental systems. Choose an organism that has not been extensively studied —something other than Arabidopsis, corn, flies, C. elegans, zebrafish, mouse, or yeast— and a trait of interest ...
... The genetic basis for organismal diversity is receiving increasing attention, aided by the development of new experimental systems. Choose an organism that has not been extensively studied —something other than Arabidopsis, corn, flies, C. elegans, zebrafish, mouse, or yeast— and a trait of interest ...
Chapter 1: Even fish obey Mendel`s laws
... cakes. DNA specifies how to build a hemoglobin molecule or other biological structure, but also determines when and where in the organism the construction is to take place. In most animals, the vast majority of the tens of thousands of genes are carried on chromosomes that are located in the nucleus ...
... cakes. DNA specifies how to build a hemoglobin molecule or other biological structure, but also determines when and where in the organism the construction is to take place. In most animals, the vast majority of the tens of thousands of genes are carried on chromosomes that are located in the nucleus ...
1 Sensitivity analysis We performed a detailed sensitivity analysis in
... more similar to the other Booleanized phenotype it becomes when the two phenotypes are complementary. This effect was not observed in the Th1-Th2 example due to the low complementarity between the two phenotypes. Of course, this situation never happens with ‘missing’ information. The robustness agai ...
... more similar to the other Booleanized phenotype it becomes when the two phenotypes are complementary. This effect was not observed in the Th1-Th2 example due to the low complementarity between the two phenotypes. Of course, this situation never happens with ‘missing’ information. The robustness agai ...
De novo assembled expressed gene catalog of a
... contigs contained more full-length gene models than current publicly available coding sequence for Eucalyptus. Supplemental Table S3. Summary of de novo assembly statistics for different classes of annotated contigs. At – Arabidopsis thaliana, Pt – Populus trichocarpa, Vv – Vitis Vinifera. A positiv ...
... contigs contained more full-length gene models than current publicly available coding sequence for Eucalyptus. Supplemental Table S3. Summary of de novo assembly statistics for different classes of annotated contigs. At – Arabidopsis thaliana, Pt – Populus trichocarpa, Vv – Vitis Vinifera. A positiv ...
Genomic imprinting and kinship in the social Hymenoptera: What
... and may be quite limited in some cases. Workers, on the other hand, primarily exert control over the secondary sex-investment ratio, although they are capable of producing male eggs in many species (Ratnieks et al., 2006). The mechanisms, and probably also the genes involved, therefore, at least par ...
... and may be quite limited in some cases. Workers, on the other hand, primarily exert control over the secondary sex-investment ratio, although they are capable of producing male eggs in many species (Ratnieks et al., 2006). The mechanisms, and probably also the genes involved, therefore, at least par ...
Candidate gene analysis in a case of congenital absence of the
... evidence of hyperandrogenism, we aimed to search for mutations in the HOXA gene family (HOXA10, HOXA11, and HOXA13). In the present case, we report the absence of endometrium and lumen in a small complete bicornuate uterus in the spectrum of presentation of MRKH and non communicating uterine horns, ...
... evidence of hyperandrogenism, we aimed to search for mutations in the HOXA gene family (HOXA10, HOXA11, and HOXA13). In the present case, we report the absence of endometrium and lumen in a small complete bicornuate uterus in the spectrum of presentation of MRKH and non communicating uterine horns, ...
Genetic of Non-syndromic Cleft Lip and Palate
... proposed role of the SMIR domain of IRF6 in mediating interactions between IRFs and Smads, a family of transcription factors known to transduce TGF-β signals [25]. It has been shown that integration of IRF6 and the Notch ligand Jagged 2 function is essential for the control of palatal adhesion and f ...
... proposed role of the SMIR domain of IRF6 in mediating interactions between IRFs and Smads, a family of transcription factors known to transduce TGF-β signals [25]. It has been shown that integration of IRF6 and the Notch ligand Jagged 2 function is essential for the control of palatal adhesion and f ...
Learning goals: Clickers - "the greatest new teaching tool since chalk:"
... Then step through the entire sequence of steps with clicker questions to test comprehension You transfect ES cells from a black mouse (dominant marker) with this construct : Neor TK YFG YFG Following transfection, the culture will include a) cells that have lost the transgene without inserting it b) ...
... Then step through the entire sequence of steps with clicker questions to test comprehension You transfect ES cells from a black mouse (dominant marker) with this construct : Neor TK YFG YFG Following transfection, the culture will include a) cells that have lost the transgene without inserting it b) ...
Differential Expression II
... Adding Power by Regularization The idea comes from what is called regularization in matrix inversion - adding a small constant before taking inverses to avoid dividing by numbers close to zero. So, we might replace Sx2 by Sx2+s where s is some small number. What number should be chosen? Usually s i ...
... Adding Power by Regularization The idea comes from what is called regularization in matrix inversion - adding a small constant before taking inverses to avoid dividing by numbers close to zero. So, we might replace Sx2 by Sx2+s where s is some small number. What number should be chosen? Usually s i ...
Enzyme Mechanisms - Illinois Institute of Technology
... To generate diversity To probe the essentiality of specific genes To examine particular segments of genes To alter properties of DNA or its mRNA transcript or a translated protein To provide information and material for gene therapy ...
... To generate diversity To probe the essentiality of specific genes To examine particular segments of genes To alter properties of DNA or its mRNA transcript or a translated protein To provide information and material for gene therapy ...
Journeys into the genome of cancer cells
... genetics of breast cancer susceptibility in collaboration with epidemiologists Doug Easton and Julian Peto. It had been recognized for many years that breast cancer cases clustered in some families. When I started, the field had just been set alight by the discovery, through genetic linkage analysis ...
... genetics of breast cancer susceptibility in collaboration with epidemiologists Doug Easton and Julian Peto. It had been recognized for many years that breast cancer cases clustered in some families. When I started, the field had just been set alight by the discovery, through genetic linkage analysis ...
Complete Nucleotide Sequence of Saccharomyces cerevisiae
... brackets are partial elements. Column 3: Genetic names of genes previously identified. Note that one previously identified gene does not have a locus name (YHR042w) and that two genes (HXT5/YHR096c and ACT5/YHR1 29c) were named during the course of this work. Column 4: A description of the function ...
... brackets are partial elements. Column 3: Genetic names of genes previously identified. Note that one previously identified gene does not have a locus name (YHR042w) and that two genes (HXT5/YHR096c and ACT5/YHR1 29c) were named during the course of this work. Column 4: A description of the function ...
Chapter 23
... • Predicts that gene frequencies should NOT change over time as long as the HW assumptions hold (no evolution should occur). • Way to calculate gene frequencies through time. ...
... • Predicts that gene frequencies should NOT change over time as long as the HW assumptions hold (no evolution should occur). • Way to calculate gene frequencies through time. ...
scylla and charybde, homologues of the human apoptotic gene
... Scylla and Charybde are homologous to RTP801, a regulator of apoptosis in humans The scyl gene codes for a 630 amino acid protein; its sequence is most closely related to the Drosophila gene chrb. The predicted scyl and chrb gene products share 64% identity and 76% similarity over 125 amino acids at ...
... Scylla and Charybde are homologous to RTP801, a regulator of apoptosis in humans The scyl gene codes for a 630 amino acid protein; its sequence is most closely related to the Drosophila gene chrb. The predicted scyl and chrb gene products share 64% identity and 76% similarity over 125 amino acids at ...
Live Cell Luciferase Assay Kit
... measured by one of the techniques described in section I. For standardization, control reactions with purified enzyme are recommended, since the kinetics of the light reaction using a crude enzyme preparation is quite different from those observed with the purified crystalline luciferase enzyme. The ...
... measured by one of the techniques described in section I. For standardization, control reactions with purified enzyme are recommended, since the kinetics of the light reaction using a crude enzyme preparation is quite different from those observed with the purified crystalline luciferase enzyme. The ...
What is DNA?
... ELONGATION: more amino acids are added and connected together to form a polypeptide, as specified by the mRNA sequence. an incoming amino-acyl-tRNA (lets call this AA2-tRNA2) recognizes the codon in the A site and binds there. a peptide bond is formed between the new amino acid and the growing polyp ...
... ELONGATION: more amino acids are added and connected together to form a polypeptide, as specified by the mRNA sequence. an incoming amino-acyl-tRNA (lets call this AA2-tRNA2) recognizes the codon in the A site and binds there. a peptide bond is formed between the new amino acid and the growing polyp ...
When the individual organism develops from a fertilized egg, the
... X chroniosomes that makes a fly female; if there is only one X, the fly will be male. Thc ring X chromosome has the property that it may get lost during nuclear division in the developing egg, so that some tissues retain only one X chromosome while others have both. Such a mosaic fly is a system in ...
... X chroniosomes that makes a fly female; if there is only one X, the fly will be male. Thc ring X chromosome has the property that it may get lost during nuclear division in the developing egg, so that some tissues retain only one X chromosome while others have both. Such a mosaic fly is a system in ...
Results
... seven different results. For the unsupervised part, there are four methods including Principal component analysis (PCA), Laplacian score, variance and Spectral feature selection. The last three methods prioritize the importance of each dimension, but PCA is slightly different from that, it merges or ...
... seven different results. For the unsupervised part, there are four methods including Principal component analysis (PCA), Laplacian score, variance and Spectral feature selection. The last three methods prioritize the importance of each dimension, but PCA is slightly different from that, it merges or ...
TimeClust: a clustering tool for gene expression time series
... TimeClust refer to genes involved in the human cell cycle. The data were collected by Whitfield et al. and are described in [1]. Original data are available for download at the site http://genome-www.stanford.edu/Human-CellCycle/Hela. Whitfield et al. performed different experiments in which they us ...
... TimeClust refer to genes involved in the human cell cycle. The data were collected by Whitfield et al. and are described in [1]. Original data are available for download at the site http://genome-www.stanford.edu/Human-CellCycle/Hela. Whitfield et al. performed different experiments in which they us ...
Identification of three MADS‐box genes expressed in sunflower
... Arabidopsis AGAMOUS (AG). These genes were isolated from homeotic mutants defective in the speci®cation of ¯oral organ identity. Subsequent work revealed the existence of a large family of MADS-box containing genes in plants. Although initially found in ¯oral tissues, it was later established that t ...
... Arabidopsis AGAMOUS (AG). These genes were isolated from homeotic mutants defective in the speci®cation of ¯oral organ identity. Subsequent work revealed the existence of a large family of MADS-box containing genes in plants. Although initially found in ¯oral tissues, it was later established that t ...
Where Do New Genes Come From? A Computational Analysis of
... What is the probability that at least k genes form a max-gap cluster in both genomes? Assuming identical gene content, the probability of finding a max-gap cluster of size at least k is always one! ...
... What is the probability that at least k genes form a max-gap cluster in both genomes? Assuming identical gene content, the probability of finding a max-gap cluster of size at least k is always one! ...
Site-specific recombinase technology
Nearly every human gene has a counterpart in the mouse (regardless of the fact that a minor set of orthologues had to follow species specific selection routes). This made the mouse the major model for elucidating the ways in which our genetic material encodes information. In the late 1980s gene targeting in murine embryonic stem (ES-)cells enabled the transmission of mutations into the mouse germ line and emerged as a novel option to study the genetic basis of regulatory networks as they exist in the genome. Still, classical gene targeting proved to be limited in several ways as gene functions became irreversibly destroyed by the marker gene that had to be introduced for selecting recombinant ES cells. These early steps led to animals in which the mutation was present in all cells of the body from the beginning leading to complex phenotypes and/or early lethality. There was a clear need for methods to restrict these mutations to specific points in development and specific cell types. This dream became reality when groups in the USA were able to introduce bacteriophage and yeast-derived site-specific recombination (SSR-) systems into mammalian cells as well as into the mouse