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LINEs
LINEs

... Transposons can be used to transfer DNA between bacterial cells Transposons (pink) integrate into new sites on the chromosome or plasmids by non-homologous recombination. Integrons (dark green) use similar mechanisms to exchange single gene cassettes (brown). ...
Mendelian Genetics
Mendelian Genetics

... Terms to Know and Use • Gene – A DNA blueprint controlling synthesis of a protein • Trait - variant for a gene: i.e. a purple flower, determined by alleles • Dominant trait - expressed over recessive trait when both are present • Recessive trait - not expressed when the dominant trait is present • ...
Principles of Heredity
Principles of Heredity

... the same order, but may have different forms of a gene at the same locus • Alleles = alternative forms of a gene – Dominant allele masks other alleles – Recessive allele is masked • Gene = sequence of DNA that codes for a protein, gives rise to physical trait ...
Exam 2 Full v3 Bio200 Win16
Exam 2 Full v3 Bio200 Win16

... In the diagram, a snapshot of a single chromosome is shown along with RNA polymerase and ribosomes. There are RNAs of various sequences, as well as three different proteins. Use this diagram to answer the questions on pages 2-3. /5 1a) At the moment of this snapshot shown to the right, which of the ...
IAP workshop, Ghent, Sept.
IAP workshop, Ghent, Sept.

... mixed-model approach used here for association mapping analysis with Kinship matrix included are more appropriate than other recent methods in identifying cis-regulated genes ( p-values more reliable).  Each step’s statistical method is controlled in a more accurate way to specify statistical signi ...
PDF
PDF

... highlights the differences in database builds. As discussed above, there are 43,037 and 53,215 unique gene symbols and synonyms respectively in Hg19, compared to 38,586 and 53,475 in Hg18, with 23,325 gene symbols overlapping between the two versions of the human genome builds. Tables 1 and 2 illust ...
An excitingly predictable `omic future - Development
An excitingly predictable `omic future - Development

... in at least two model organisms (Chen et al., 2005; Mineno et al., 2006), an undertaking that has proven to be essential for the continued progress of developmental biology. However, medical genetics, cancer genetics, microbiology and virology made much bigger strides using the same technology. This ...
Biotechnology Part 1
Biotechnology Part 1

... Plasmid: Small circular loop of DNA, outside of the main chromosome Bioinformatics: Use computers to sort through data ...
Supplemental Data
Supplemental Data

... Supplemental Figure S3. Positive colonies of transformants, PCR and RT-PCR analysis of ble-egfp DNA and transcripts in D. bardawil cells electroporated with pZBET. Transformed cells were spread onto Zeocin selective plate immediately (A). When colonies appeared, single colony was transferred to sel ...
Adult stem cells
Adult stem cells

... lung cells growing in the lab, a component of tobacco smoke, BPDE, binds to DNA within a gene called p53, which codes for a protein that normally helps suppress the formation of tumors. • This work directly linked a chemical in tobacco smoke with the formation of human lung tumors. ...
Comparison of two known chromosomal rearrangements in the
Comparison of two known chromosomal rearrangements in the

... regions (9, 10). Also, genes that are more proximal to the 5’ LCR (locus control regions) seem to have a transcriptional advantage over more distant genes (11). This supports the observation that the beta-delta fusion gene is expressed at higher levels resulting in an ...
The Taste of Cats - University of Maryland, College Park
The Taste of Cats - University of Maryland, College Park

... Brand, wanted to figure out why cats are cannot taste sweets.  She compared sweet receptor genes in cats with the sequence and structure of the same genes in dogs, humans, mice and rats. ...
8 Expression and Modification of Recombinant Proteins
8 Expression and Modification of Recombinant Proteins

... promoters and translation signals are different...they are not exchangeable You therefore can’t simply put a eukaryotic promoter into bacteria and expect it to function ...
supervised-i
supervised-i

... BME 230 ...
Bioinformatics tools and methods
Bioinformatics tools and methods

... arranged in a hierarchical tree structure. ...
Nature, Nurture and Human Diversity
Nature, Nurture and Human Diversity

... How stability of our temperament illustrates the influence of heredity on development • Temperament: characteristic emotional excitability and intensity – Relaxed and cheerful v. tense and irritable • Ex: emotionally intense preschoolers tend to be relatively intense young adults • Compared with fr ...
Chapter 18~Regulaton of Gene Expression
Chapter 18~Regulaton of Gene Expression

... – usually functions in catabolic pathways, • digesting nutrients to simpler molecules ...
Katie-Arabidopsis
Katie-Arabidopsis

... • No immediate agricultural importance and is not thought to cure any disease • Prolific seed production and easy cultivation in restricted space • A large number of mutant lines and genomic resources ...
Diapositiva 1
Diapositiva 1

... Epigenetics in prostate cancer ...
Notes and Study Guide for weeks 8
Notes and Study Guide for weeks 8

... chromosome are both considered sex-linked traits. Why is that? Fig. 7.24. If a man has a particular allele for a gene on his Y chromosome, will all of his sons inherit this allele? Will any of his daughters inherit this allele? K. Genes that are located close together on a chromosome are less likely ...
No Slide Title
No Slide Title

... •Others libraries may have gene in lower abundance, but they are specific to that library. Its possible to find the specific genes by ‘subtracting’ the sequences present in other tissues. E.g. A library from pathogen-infected tissue with the sequences from uninfected tissue subtracted out. •Expressi ...
Contributions of candidate-gene research to understanding the role of
Contributions of candidate-gene research to understanding the role of

... contrasted selective pressures that may have increased general levels of diversity at candidate genes. Drought response is a very complex trait, as forest trees use very different strategies to confront drought. In addition, response to drought involves several cross-talking and incompletely known m ...
Lesson 13: Polygenic Inheritance student notes
Lesson 13: Polygenic Inheritance student notes

HSP-70 AND THERMOPROTECTION
HSP-70 AND THERMOPROTECTION

... • 40°C (104°F) for a couple of hours should be a mild enough temperature to initiate thermoprotection but not hot enough to kill the mealworms. 45°C (113°F) for one hour is good for a temperature that will normally hurt them. These are not exact yet (I’ll work on it), so you should probably play wi ...
Factors that influence gene expression
Factors that influence gene expression

... to specific protein targets. There are several types of ubiquitin modification, and these may have different effects on target proteins. The best known is the polyubiquitin chain, which targets proteins for proteasomal degradation. The polyubiquitin chain begins with a ubiquitin conjugated at its C ...
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Gene expression profiling



In the field of molecular biology, gene expression profiling is the measurement of the activity (the expression) of thousands of genes at once, to create a global picture of cellular function. These profiles can, for example, distinguish between cells that are actively dividing, or show how the cells react to a particular treatment. Many experiments of this sort measure an entire genome simultaneously, that is, every gene present in a particular cell.DNA microarray technology measures the relative activity of previously identified target genes. Sequence based techniques, like serial analysis of gene expression (SAGE, SuperSAGE) are also used for gene expression profiling. SuperSAGE is especially accurate and can measure any active gene, not just a predefined set. The advent of next-generation sequencing has made sequence based expression analysis an increasingly popular, ""digital"" alternative to microarrays called RNA-Seq. However, microarrays are far more common, accounting for 17,000 PubMed articles by 2006.
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