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Shah - Buffalo Ontology Site
Shah - Buffalo Ontology Site

... A hypothesis consists of at least one event stream An event stream is a sequence of one or more events or event streams with logical joints (or operators) between them. An event has exactly one agent_a, exactly one agent_b and exactly one operator (i.e. a relationship between the two agents). It als ...
Finding motifs in preomoters
Finding motifs in preomoters

... K = K(t) be the number of sequences of length L (out of the 4L) with a score above t.  We have a branch and bound algorithm for enumerating them in time linear in K.  Problem : In some cases K might be too large. For example, Suppose L=20, and only one of a thousand sequences of length L has a sco ...
July, 2004 - Think Muscle
July, 2004 - Think Muscle

... how muscular we are. We focus on these two things because ultimately we have control over them. We can change how muscular we are by training with weights. We can change how fat we are by eating less and moving more. The problems start when we get impatient and try to get bigger and leaner at the sa ...
Institut für Humangenetik - UniversitätsKlinikum Heidelberg
Institut für Humangenetik - UniversitätsKlinikum Heidelberg

... In case a disease-causing genetic variant (e.g. mutation) is detected, the diagnosis can generally be considered very reliable. If no genetic variation (mutation) can be identified as the cause of your disease there is still a possibility of a causative mutation in the examined gene / genes or in on ...
Cynthia Smith
Cynthia Smith

... • accommodate bio-specific terms • computationally useful • human friendly ...
You Light Up My Life
You Light Up My Life

... • Defective channel protein in membrane of cells in exocrine glands • Glands secrete abnormally thick, gluey mucus • Interferes with breathing, pancreatic function ...
An Investigation into the Genomic Evolution of the Histone Gene
An Investigation into the Genomic Evolution of the Histone Gene

... Gene Family Kaupp, Kristin, Dr. Marlene Snyder Department of Biology, Acadia University, Wolfville, NS The extremely high level of within and between species similarity of the histone gene family has lead to the proposal that the histone gene family is subject to concerted evolution. Both mechanisms ...
The Human Genome Project, Modern Biology, and Mormonism: A
The Human Genome Project, Modern Biology, and Mormonism: A

... important, as many thousands of lives are lost each year due to unforeseen drug interactions and toxicity. To analyze drug toxicity, a copy of each gene found within a person's genome is attached to a glass slide. Cells isolated from the person's body can then be tested with potential drugs by first ...
Week 8
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... the maximal wild-type rate • This is achieved in a few hundreds of generations ...
Section J Analysis and Uses of Cloned DNA
Section J Analysis and Uses of Cloned DNA

... • Length of target sequences:  Short target sequences amplify more easily, so often this distance is less than 500 bp, but, with optimization, PCR can amplify fragments over 10 kb in length. • Primer design: – The region to be amplified should be inspected for two sequences of  about 20 nt with a ...
Slide 1
Slide 1

...  Some regions of the genome appear to accumulate changes at constant rates  Molecular clocks can be calibrated in real time by graphing the number of nucleotide differences against the dates of evolutionary branch points known from the fossil record – Molecular clocks are used to estimate dates of ...
Standard 3—Genetics
Standard 3—Genetics

... If ______ copies of an autosomal chromosome fail to separate during meiosis, an individual may be born with three copies of a chromosome. Down syndrome involves three copies of chromosome _____. ...
University of Groningen A Branched Biosynthetic Pathway Is
University of Groningen A Branched Biosynthetic Pathway Is

... Other than for strictly personal use, it is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), unless the work is under an open content license (like Creative Commons). Take-down policy If you believe that this d ...
Isabel Skidmore - life.illinois.edu
Isabel Skidmore - life.illinois.edu

Chapter 13 - Gene Function
Chapter 13 - Gene Function

... Why do we need to make RNA from DNA first? Believe it or not it’s a great regulatory site If we don’t need a particular protein, do our cells want to waste making it? How does a cell know when to express a particular gene (DNA sequence) & how does the cell control this expression? The ability to for ...
Genoombrowsers - Radboud Universiteit
Genoombrowsers - Radboud Universiteit

... • Sequence variations within a species • Similar to mutations, but are simultaneously present in the population, and generally have ...
One Step Quantitative Real-Time PCR Protocol
One Step Quantitative Real-Time PCR Protocol

... crucial for reverse transcription. Doing NRC once is enough, if the same RNA samples are used in experiments with different genes. In addition, NTC must be included in each plate every time for each tested gene. This is a good control for checking for any contamination in primer/probe mix or formati ...
Supplementary Figure Legends (doc 38K)
Supplementary Figure Legends (doc 38K)

... Fig. S12. Ectopic expression of Sp1 promotes the binding of Sp1 to p53 target genes and p53-mediated pro-apoptotic transcriptional repression in MCF-7 cells upon nutlin treatment. (A) Binding of Sp1 to selected genes (described in Fig. 4) was assessed by ChIP-PCR in nutlin-treated MCF-7 cells with o ...
Genetic Analysis of Peas and Humans
Genetic Analysis of Peas and Humans

... half of the F2 of a red x white cross have pink flowers. These plants have only one functional allele, R, of a gene encoding an enzyme required for making the red flower pigment. Two copies of R are necessary to make enough pigment for red flowers.  In peas, half the normal dosage of an analogous g ...
Chapter 6 Genetics and Inheritance - Linn
Chapter 6 Genetics and Inheritance - Linn

... Recessive = green (y) ...
BIO 274-01 Exam 3 Name 1. The SAM has three distinct zones
BIO 274-01 Exam 3 Name 1. The SAM has three distinct zones

... d. none of the above 19. The epidermis a. covers the entire surface of the leaf b. consists of guard cells, epidermal cells and trichomes c. both a and b d. neither a nor b 20. The first sign of leaf initiation is the appearance of a. the leaf bud b. the leaf primordia c. the cotyledons d.. the apic ...
Lacroix_Insyght navigating amongst abundant - Migale
Lacroix_Insyght navigating amongst abundant - Migale

... Moreover, shared synteny may indicate a relationship between gene products such as protein–protein interaction (9) or functional coupling (10,11). Transcriptional activity has also been correlated to conserved synteny in expression pattern and transcriptional regulation studies (12,13). Several anno ...
Chapter 4: Sex Determination and Sex Chromosomes
Chapter 4: Sex Determination and Sex Chromosomes

... Overview For the most part, genes are responsible for the production of proteins, which in turn determine the phenotype of the individual. In diploid organisms, autosomal genes are inherited in pairs for all members of the species. However, for genes on the sex chromosomes, the sex of the individual ...
Inheritance Patterns - Osteogenesis Imperfecta Foundation
Inheritance Patterns - Osteogenesis Imperfecta Foundation

... Inheritance Patterns-How OI Occurs in Families Background. In general, we have two copies of each gene. One copy we inherited from our father and one we inherited from our mother. Some genetic conditions occur because one copy of one of these genes is altered (this is called a dominant condition) an ...
Planet Earth and Its Environment A 5000-million year
Planet Earth and Its Environment A 5000-million year

... It is an advancement on the reproductive technologies that we have already discussed, as it allows specific desirable genes to be moved from one species to another. Genes can be ‘cut and pasted’— removed from the cells of one organism and inserted into the genome of another organism, where they beco ...
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Gene expression profiling



In the field of molecular biology, gene expression profiling is the measurement of the activity (the expression) of thousands of genes at once, to create a global picture of cellular function. These profiles can, for example, distinguish between cells that are actively dividing, or show how the cells react to a particular treatment. Many experiments of this sort measure an entire genome simultaneously, that is, every gene present in a particular cell.DNA microarray technology measures the relative activity of previously identified target genes. Sequence based techniques, like serial analysis of gene expression (SAGE, SuperSAGE) are also used for gene expression profiling. SuperSAGE is especially accurate and can measure any active gene, not just a predefined set. The advent of next-generation sequencing has made sequence based expression analysis an increasingly popular, ""digital"" alternative to microarrays called RNA-Seq. However, microarrays are far more common, accounting for 17,000 PubMed articles by 2006.
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