The universal reagent for genome tailoring
... Another variation of this approach is pronuclear injection of a Cre expression vector, and the resultant transient Cre expression does its job during preimplantation development (Araki et al., 1995). Injecting Cre RNA or protein itself may also work. These latter three means are obviously more tedio ...
... Another variation of this approach is pronuclear injection of a Cre expression vector, and the resultant transient Cre expression does its job during preimplantation development (Araki et al., 1995). Injecting Cre RNA or protein itself may also work. These latter three means are obviously more tedio ...
Genetic Polymorphism of Human CYP2E1
... was detected by Northern blot, and the CYP2E1 apoprotein levels were quantified by Western blot. The CYP2E1-dependent catalytic activities were determined by measuring NADPHdependent hydroxylation of chlorzoxazone in 10,000 3 g supernatants of the transfected cells. All constructs except the pCMV4 v ...
... was detected by Northern blot, and the CYP2E1 apoprotein levels were quantified by Western blot. The CYP2E1-dependent catalytic activities were determined by measuring NADPHdependent hydroxylation of chlorzoxazone in 10,000 3 g supernatants of the transfected cells. All constructs except the pCMV4 v ...
NanoString™: User Guide | nCounter® Expression Data Analysis
... Each miRGE CodeSet contains probes designed against sixteen ERCC transcript sequences. Six of these sequences are used as positive hybridization controls, two are used as ligation controls and eight are designed as negative controls. For each positive hybridization control, in-vitro transcribed RNA ...
... Each miRGE CodeSet contains probes designed against sixteen ERCC transcript sequences. Six of these sequences are used as positive hybridization controls, two are used as ligation controls and eight are designed as negative controls. For each positive hybridization control, in-vitro transcribed RNA ...
pdf
... RECOMBINATION OF DNA The previous chapter on mutation and repair of DNA dealt mainly with small changes in DNA sequence, usually single base pairs, resulting from errors in replication or damage to DNA. The DNA sequence of a chromosome can change in large segments as well, by the processes of recomb ...
... RECOMBINATION OF DNA The previous chapter on mutation and repair of DNA dealt mainly with small changes in DNA sequence, usually single base pairs, resulting from errors in replication or damage to DNA. The DNA sequence of a chromosome can change in large segments as well, by the processes of recomb ...
Publication - The University of Texas Health Science Center at
... supports a strong genetic link to scleroderma. Also reviewed are the family and twin studies that suggest a genetic component in scleroderma, and recent genetic-association studies implicating specific genes in the pathogenetic triad of autoimmunity, endothelial dysfunction, and fibroblast activation. ...
... supports a strong genetic link to scleroderma. Also reviewed are the family and twin studies that suggest a genetic component in scleroderma, and recent genetic-association studies implicating specific genes in the pathogenetic triad of autoimmunity, endothelial dysfunction, and fibroblast activation. ...
Gene Section ATF4 (activating transcription factor 4 (tax responsive enhancer element B67)) -
... provides a mechanistic link between nutrients, insulin resistance, and diabetes, and has been described as a major mediator of nutrition-sensing response pathway, regulating the expression of asparagine synthetase (ASNS). In addition to regulating the expression of ASNS during lack of nutrition, ATF ...
... provides a mechanistic link between nutrients, insulin resistance, and diabetes, and has been described as a major mediator of nutrition-sensing response pathway, regulating the expression of asparagine synthetase (ASNS). In addition to regulating the expression of ASNS during lack of nutrition, ATF ...
Coordination of replication and transcription along a Drosophila
... arrays of cDNAs have demonstrated a correlation between time of replication and the probability that a specific gene is expressed, it remained to be determined what step(s) in the replication initiation process are influenced by transcription. Similarly, because the prior studies lacked contiguous i ...
... arrays of cDNAs have demonstrated a correlation between time of replication and the probability that a specific gene is expressed, it remained to be determined what step(s) in the replication initiation process are influenced by transcription. Similarly, because the prior studies lacked contiguous i ...
Gene Section PAX8 (paired box 8) Atlas of Genetics and Cytogenetics
... PAX8/PPARgamma1 has been identified in thyroid tumors of follicular cell deriva-tion characterized by a well developed follicular pattern of growth. These tumors are usually follicular carcinomas but may also be follicular variant papillary carcinomas or follicular adenomas. It has therefore been su ...
... PAX8/PPARgamma1 has been identified in thyroid tumors of follicular cell deriva-tion characterized by a well developed follicular pattern of growth. These tumors are usually follicular carcinomas but may also be follicular variant papillary carcinomas or follicular adenomas. It has therefore been su ...
here
... OCD is Polygenic Some cases of OCD, and in particular hoarding disorder, seem to be associated However, like many conditions, OCD seems to be polygenic. This means that OCD is not caused by one single gene but that several genes are with impaired decision making. This in turn may be associated with ...
... OCD is Polygenic Some cases of OCD, and in particular hoarding disorder, seem to be associated However, like many conditions, OCD seems to be polygenic. This means that OCD is not caused by one single gene but that several genes are with impaired decision making. This in turn may be associated with ...
Figure 20-6
... • It can be efficient as long as environmental conditions don’t change • However, under changing environmental conditions, organisms that undergo sexual reproduction usually have an advantage © 2012 Pearson Education, Inc. ...
... • It can be efficient as long as environmental conditions don’t change • However, under changing environmental conditions, organisms that undergo sexual reproduction usually have an advantage © 2012 Pearson Education, Inc. ...
Plant centromeres: structure and control Eric J Richards and R Kelly
... arrays map genetically to the intervals expressing meiotic centromere function delimited by tetrad analysis [29•,30•]. A higher resolution mapping of centromere function relative to centromeric repeat arrays was accomplished by physical mapping in maize. Kaszás and Birchler, using chromosome breaka ...
... arrays map genetically to the intervals expressing meiotic centromere function delimited by tetrad analysis [29•,30•]. A higher resolution mapping of centromere function relative to centromeric repeat arrays was accomplished by physical mapping in maize. Kaszás and Birchler, using chromosome breaka ...
Finishing the Human Genome
... Doug Brutlag Professor Emeritus of Biochemistry & Medicine Stanford University School of Medicine Doug Brutlag 2011 ...
... Doug Brutlag Professor Emeritus of Biochemistry & Medicine Stanford University School of Medicine Doug Brutlag 2011 ...
Shoot Branching and Leaf Dissection in Tomato Are
... and leaflets are fused. Ectopic lamina growth on the rachis can also be induced by auxin application to young leaf primordia (Koenig et al., 2009). Expression of KNOX genes in ectopic positions, as in the mutant Mouse ears (Me; Parnis et al., 1997) or when using transgenic approaches (Hareven et al. ...
... and leaflets are fused. Ectopic lamina growth on the rachis can also be induced by auxin application to young leaf primordia (Koenig et al., 2009). Expression of KNOX genes in ectopic positions, as in the mutant Mouse ears (Me; Parnis et al., 1997) or when using transgenic approaches (Hareven et al. ...
Developmental buffering: how many genes?
... Although most studies of Hsp90 and phenotypic variability have been conducted in Drosophila, similar patterns appear also to hold for other organisms. In zebrafish, pharmacological inhibition and knockdown of Hsp90 induced a range of specific abnormalities depending on the genotype (Yeyati et al. 2007 ...
... Although most studies of Hsp90 and phenotypic variability have been conducted in Drosophila, similar patterns appear also to hold for other organisms. In zebrafish, pharmacological inhibition and knockdown of Hsp90 induced a range of specific abnormalities depending on the genotype (Yeyati et al. 2007 ...
Chap 13
... generation after generation – Cross-fertilized pea plants with different traits and offspring appeared in predictable patterns – Concluded that hereditary information is passed in discrete units ...
... generation after generation – Cross-fertilized pea plants with different traits and offspring appeared in predictable patterns – Concluded that hereditary information is passed in discrete units ...
Set - people.vcu.edu
... Anab7120:all0687 hupL [NiFe] uptake hydrogenase large subunit, C terminus Anab7120:all0687 hupL [NiFe] uptake hydrogenase large subunit, N terminus Anab7120:all0688 hupS [NiFe] uptake hydrogenase small subunit Anab7120:alr0692 ...
... Anab7120:all0687 hupL [NiFe] uptake hydrogenase large subunit, C terminus Anab7120:all0687 hupL [NiFe] uptake hydrogenase large subunit, N terminus Anab7120:all0688 hupS [NiFe] uptake hydrogenase small subunit Anab7120:alr0692 ...
Spectrum of Mutations in MMAB Identified by
... I would like to express my appreciation to my mentor, David Rosenblatt, for providing me the opportunity, not only once but twice, to be actively engaged in a field of personal interest. Being a member of the Rosenblatt team provides the friendship, support and unquestioning assistance of a very spe ...
... I would like to express my appreciation to my mentor, David Rosenblatt, for providing me the opportunity, not only once but twice, to be actively engaged in a field of personal interest. Being a member of the Rosenblatt team provides the friendship, support and unquestioning assistance of a very spe ...
Hox patterning of the vertebrate rib cage
... and Capecchi, 1994; Rancourt et al., 1995). As C7 and T1 are affected in both the Hox5 and the Hox6 single mutants, colinearity is not immediately apparent for these genes; however, paralogous mutants have not been examined for these groups. Mutants in the Hox7, Hox8 and Hox9 genes have also been ex ...
... and Capecchi, 1994; Rancourt et al., 1995). As C7 and T1 are affected in both the Hox5 and the Hox6 single mutants, colinearity is not immediately apparent for these genes; however, paralogous mutants have not been examined for these groups. Mutants in the Hox7, Hox8 and Hox9 genes have also been ex ...
PHANTASTICA Regulates Development of the Adaxial Mesophyll in
... show disruptions in adaxial/abaxial symmetry and differentiation of adaxial blade cell types in the abaxial domain (Lin et al., 2003). Further studies on PHAN in Antirrhinum revealed that it coded for a MYB-containing transcription factor involved not only in leaf polarity but also in elaboration of ...
... show disruptions in adaxial/abaxial symmetry and differentiation of adaxial blade cell types in the abaxial domain (Lin et al., 2003). Further studies on PHAN in Antirrhinum revealed that it coded for a MYB-containing transcription factor involved not only in leaf polarity but also in elaboration of ...
Genetics of Male Infertility - the Infertility Center of St. Louis
... markers. These translocations could conceivably be transmitted to offspring if ICSI allowed them to conceive. However, because of the limitations of the resolution of cytogenetics, and the very small percentage of these readily discernable karyotypic abnormalities found in infertile men, until recen ...
... markers. These translocations could conceivably be transmitted to offspring if ICSI allowed them to conceive. However, because of the limitations of the resolution of cytogenetics, and the very small percentage of these readily discernable karyotypic abnormalities found in infertile men, until recen ...
A Fine Physical Map of Arabidopsis thaliana Chromosome 5
... clones CIC11D9 and CIC6A9 have already been mapped on contig IV of chromosome 4.9 A PI clone, MJO17, containing the PDC1 gene also has the ATH1 gene24 which is used as a characterization marker of chromosome 4 and has also been mapped on CIC11D9 and CIC6A9.9 Therefore, we concluded that the PDC1 gen ...
... clones CIC11D9 and CIC6A9 have already been mapped on contig IV of chromosome 4.9 A PI clone, MJO17, containing the PDC1 gene also has the ATH1 gene24 which is used as a characterization marker of chromosome 4 and has also been mapped on CIC11D9 and CIC6A9.9 Therefore, we concluded that the PDC1 gen ...
Gene Expression
... Charles Yanofsky – deduced key features of relationship between nucleotides and amino acids Generated large number of trp− auxotrophic mutants in E. ...
... Charles Yanofsky – deduced key features of relationship between nucleotides and amino acids Generated large number of trp− auxotrophic mutants in E. ...
Cytoplasmic male sterility
... wild progenitor of barley (H. vulgare ssp. spontaneum). This selection, 79BS143, originates from the Southern Coastal Plain of Israel. The selection 79BS14-3 has a normal spike fertility in Finland. When 79BS14-3 was crossed by cv. Adorra, the F1 displayed partial male fertility and progeny of recur ...
... wild progenitor of barley (H. vulgare ssp. spontaneum). This selection, 79BS143, originates from the Southern Coastal Plain of Israel. The selection 79BS14-3 has a normal spike fertility in Finland. When 79BS14-3 was crossed by cv. Adorra, the F1 displayed partial male fertility and progeny of recur ...
Ethanol exposure affects gene expression in the embryonic
... R. Yelin et al. / Developmental Biology 279 (2005) 193–204 ...
... R. Yelin et al. / Developmental Biology 279 (2005) 193–204 ...