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Supplementary Table 1
Supplementary Table 1

... Supplementary Table 1 Up-regulated and down-regulated genes after the addition of 100 mM L-serine by microarray analysis Genes of strain BWG1-7A significantly up- or down-regulated during the four hours time-course of growth in the presence of 100 mM L-serine are listed in table IA and table IB resp ...
12551_2008_5_MOESM1_ESM - Springer Static Content Server
12551_2008_5_MOESM1_ESM - Springer Static Content Server

... Polypeptides. Academic, New York At the time this was the most comprehensive compilation of data available on both fibrous proteins and the methodology used to study them. It still provides an excellent summary on the structures of many of the synthetic polypeptides that were used as model protein s ...
Characterization of Phosphorylation Sites from the Activation Loop
Characterization of Phosphorylation Sites from the Activation Loop

... A kinase-dead (KD) mutant of ERK1 was created by mutating K71 of the ATP-binding subdomain II to alanine. Another mutant with the substitution of K72 to arginine was also constructed and expressed in E. coli. Purified GST-fusion ERK1 was assayed in vitro with wild-type (WT) MEK1 and a super active m ...
Jelly Facts - Institute of Food Research
Jelly Facts - Institute of Food Research

... Collagen in animal skin and bones is broken down by heat and treatment with acids and alkalis. Bonds between collagen molecules (intermolecular bonds), bonds in the molecules (intramolecular bonds) and hydrogen bonds are broken down, making gelatin. When protein loses its shape it denatures. When t ...
Characterization of Proteins Structurally Related to Human N
Characterization of Proteins Structurally Related to Human N

... association of each isoenzyme with the respective fraction was confirmed by Cellogel electrophoresis as described by Braidman et al. (1974). The recovery of protein was 75-77% of that applied (range from three experiments). Under these conditions the serologically unrelated hexosaminidase C is not r ...
article MUNs - it is only a piece of the puzzle
article MUNs - it is only a piece of the puzzle

... feeding, especially in AM / PM herd sample programs or herds milking 3X can lead to different “normal” values. ??Accuracy of the test - Because the MUN test is a NIR technology, it must be calibrated against a standard test to ensure that it is accurate. Recent work by Elliot Block and Daniel Lefebv ...
Phosphorylation of apoproteins in VLDL, and LDL by protein
Phosphorylation of apoproteins in VLDL, and LDL by protein

... for apo BlOO phosphorylated by CAMP-PKcompared with AMPPK (Figure lb). This indicates that these two kinases phosphorylate different sites. This has also been suggested by reversed phase HPLC separation of tryptic peptides from apo BlOO phosphorylated by either kinase and will be confmed when these ...
Docking with GOLD Tutorial
Docking with GOLD Tutorial

... b. the prepared 3D coordinates of your protein An example of a prepared GOLD configuration file is included as MLL1.conf in your example folder. We are going to prepare another copy of this file in the next sections of this tutorial. Have a look at the file and find the entries specifying your prote ...
Protein Function and Classification (Cont.) - EMBL-EBI
Protein Function and Classification (Cont.) - EMBL-EBI

... InterPro provides functional analysis of proteins by classifying them into families and predicting the presence of important domains and sites. It does this by combining predictive models known as protein signatures from a number of different databases (referred to as member databases) into a single ...
preprint - Human Genome Center
preprint - Human Genome Center

... are seriously needed. Two proteins are said to have similar functions if their 3-D structures are similar to each other, and structure comparison is one of the keys to the protein function analysis. There are many structure comparison algorithms for proteins [Eidhammer et al. 2000]. Many of these al ...
Williams, Brandon: A Review on Common Applications Designed to Optimize the SEQUEST Search Algorithm With Respect to Limiting False Positive Identification
Williams, Brandon: A Review on Common Applications Designed to Optimize the SEQUEST Search Algorithm With Respect to Limiting False Positive Identification

... New proteomic technologies have emerged that deal with understanding complex systems and diseases at a global level, thus allowing an expanded experimental view. Before the advent of mass spectrometry, proteomic approaches were initially explored via the use of two-dimensional gel electrophoresis (1 ...
Bryan 2012 Research Day Poster
Bryan 2012 Research Day Poster

... antimicrobial reactive oxygen species, but does upregulate a specific protein when exposed to a pathogen such as Aspergillus fumigatus. •We are collecting further data to better describe these unreported pulmonary vesicles. ...
PowerPoint - Oregon State University
PowerPoint - Oregon State University

... • Identification of ToxB homologs will be carried out by PCR. • Screening of several ascomycete isolates • Primers have been designed for the Internal Transcribed Spacer (ITS) sequence and the Ptr ToxB sequence within the ORF ...
Diapositiva 1 - ASCRS/ASOA 2008
Diapositiva 1 - ASCRS/ASOA 2008

... Both tear collection, electrophoretic fractionation and gel densitometry were performed in sextuplicate (A through F) For each tracing, the Y-axis represent band intensities. Tracings of protein migration in gel fractionation were performed from higher (left) to lower (right) molecular weights. Band ...
Population Biology of the First Replicators: On
Population Biology of the First Replicators: On

... which gives rise to the "once-forever" conX, = (B,X, dition for evolution of hypercycles discussed below. However, there are several X2 = (B2X2 problems with hypercycle (and hence protein) evolution which their work does not with Eigen's (1971) form of density reguaddress. The basic problem concerns ...
SB401, a pollen-specific protein from Solanum berthaultii
SB401, a pollen-specific protein from Solanum berthaultii

... model system for investigating the control and regulation of cell growth in plant. The cytoskeleton is crucial for the tip growth of pollen tubes. A large body of evidence has established that actin filaments are fundamental, not only for delivering substances to the pollen tube tip by cytoplasmic s ...
mechanism of the flagellar export system and its potential
mechanism of the flagellar export system and its potential

... Molecular dynamics simulations showed that the terminal region of FliS is much more flexible compared to that of stable proteins exhibiting cooperative unfolding transitions with similar temperature midpoints. Temperature increase resulted first in the shortening of helices, while their number decre ...
PowerPoint 演示文稿
PowerPoint 演示文稿

... to A-site. Energy is from GTP. 2. Peptide bond formation. After aatRNA delivery, the A- and P-site are both occupied and the two amino acids that are joined closely. The peptidyl transferase activity of the 50S subunit can now form a peptide bond ...
Elastin
Elastin

... They can be stretched to several times their normal length, but recoil to their original shape when the stretching force is relaxed. ...
The standard procedure starts with a set of sequences
The standard procedure starts with a set of sequences

You can answer the question Yourself with a few
You can answer the question Yourself with a few

... high-quality milk, but not every company does the same. To cut costs and make their powders more affordable, some manufacturers use questionable suppliers. These lesser-quality powders may also contain impurities and toxins. The easiest way to spot a powder that’s using cheap raw protein is its pric ...
Poly(Pro)II Helices in Globular Proteins: Identification and Circular
Poly(Pro)II Helices in Globular Proteins: Identification and Circular

Screening for increased protein thiol oxidation in oxidatively
Screening for increased protein thiol oxidation in oxidatively

Protein 4.2 interaction with hereditary spherocytosis mutants of the
Protein 4.2 interaction with hereditary spherocytosis mutants of the

... predominant of two protein 4.2 splice variants. Type I is the less common larger isoform and has an additional 30 amino acids near the N-terminus [11]. Amino acid numbering of protein 4.2 in the present paper will be according to the Type II isoform. Protein 4.2 is a member of the TG (transglutamina ...
PDF
PDF

... later. The synthesis and modification of proteins during these first few hours of maturation were studied by short-term radiolabelling of oocytes with [35S]methionine and [32P]phosphate followed by rapid assessment of their precise nuclear configuration. Changes in phosphorylation of two polypeptide ...
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Protein design

This article refers to rational protein design. For the broader engineering of proteins see protein engineering.Protein design is the rational design of new protein molecules to fold to a target protein structure, with the ultimate goal of designing novel function and/or behavior. Proteins can be designed from scratch (de novo design) or by making calculated variations on a known protein structure and its sequence (known as protein redesign). Rational protein design approaches make protein-sequence predictions that will fold to specific structures. These predicted sequences can then be validated experimentally through methods such as peptide synthesis, site-directed mutagenesis, or artificial gene synthesis.Rational protein design dates back to the mid-1970s, although initial protein design approaches were based mostly on sequence composition and did not account for specific interactions between side-chains at the atomic level. Recently, however, improvements in molecular force fields, protein design algorithms, and structural bioinformatics, such as libraries of amino acid conformations, have enabled the development of advanced computational protein design tools. These computational tools can make complex calculations on protein energetics and flexibility, and perform searches over enormous configuration spaces, which would be unfeasible to perform manually. Thanks to the development of computational protein design programs and important successes in the field (e.g., see examples below), rational protein design has become one of the most important tools in protein engineering.
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