lab manager`s independent guide to purchasing an optical
... Advanced polarizing microscopes are suitable for digital imaging, including options for fluorescence and darkfield. They tend to offer magnification in the range 40x to 5000x for observational applications in cell biology, molecular pathology, cytology, pathology, genetics and neurobiology. ...
... Advanced polarizing microscopes are suitable for digital imaging, including options for fluorescence and darkfield. They tend to offer magnification in the range 40x to 5000x for observational applications in cell biology, molecular pathology, cytology, pathology, genetics and neurobiology. ...
Isolation and Characterization of a Novel Ribosome
... Ribosome-inactivating proteins are N-glycosidases that remove a specific adenine from the sarcin/ricin loop of the large rRNA, thus arresting protein synthesis at the translocation step. In the present study, a novel type I ribosome-inactivating protein, termed PAP-H, was purified from Agrobacterium ...
... Ribosome-inactivating proteins are N-glycosidases that remove a specific adenine from the sarcin/ricin loop of the large rRNA, thus arresting protein synthesis at the translocation step. In the present study, a novel type I ribosome-inactivating protein, termed PAP-H, was purified from Agrobacterium ...
Laboratory Exercise # 6: Gram Stain Purpose: The student will
... laboratory to categorize bacteria. The primary stain is the cationic dye Crystal violet and the secondary stain is the cationic dye Safranin. Since both stains are cationic, they are both attracted to the negatively charged particles in the bacterial cell wall. So what causes some bacteria to retain ...
... laboratory to categorize bacteria. The primary stain is the cationic dye Crystal violet and the secondary stain is the cationic dye Safranin. Since both stains are cationic, they are both attracted to the negatively charged particles in the bacterial cell wall. So what causes some bacteria to retain ...
Ultrastructural immunocytochemical localization of
... (iii) Stage 5. In view of the relative complexity of the stage-5 embryo, sections through at least five different regions were examined on each embryo, as shown in Fig. 1. Sections were made through the primitive streak, at various levels, including areas lateral to this structure (Fig. 8). In addit ...
... (iii) Stage 5. In view of the relative complexity of the stage-5 embryo, sections through at least five different regions were examined on each embryo, as shown in Fig. 1. Sections were made through the primitive streak, at various levels, including areas lateral to this structure (Fig. 8). In addit ...
Cancer - inoncology
... can only be prevented by screening and appropriate early intervention Cancer caused by extrinsic factors can be prevented by reducing or eliminating exposure to these factors (e.g. chemicals, tobacco, radiation, viruses) ...
... can only be prevented by screening and appropriate early intervention Cancer caused by extrinsic factors can be prevented by reducing or eliminating exposure to these factors (e.g. chemicals, tobacco, radiation, viruses) ...
A Definitive, Rapid Alternative to the Gram Stain Assay
... The Gram stain assay differentiates bacteria into two groups. Gram positive organisms have high levels of peptidoglycan in their cell walls, which retain the primary crystal violet stain and appear purple. The Gram negative cell wall contains small levels of peptidoglycan but also contains an outer ...
... The Gram stain assay differentiates bacteria into two groups. Gram positive organisms have high levels of peptidoglycan in their cell walls, which retain the primary crystal violet stain and appear purple. The Gram negative cell wall contains small levels of peptidoglycan but also contains an outer ...
CLASSIFICATION AND REPORTING OF COLONIC POLYPS PART I
... •All other polyps are tubulovillous adenoma •Criteria can only be used reliably in polypectomy and resection specimen or tissue fragments large enough to assess the various proportions present •“At least tubulovillous”: small biopsy of a large polyp when at least one villous is present •It may be di ...
... •All other polyps are tubulovillous adenoma •Criteria can only be used reliably in polypectomy and resection specimen or tissue fragments large enough to assess the various proportions present •“At least tubulovillous”: small biopsy of a large polyp when at least one villous is present •It may be di ...
Enhancers of HIV Infection Phosphatase Form Amyloidogenic
... investigate whether the fibrils increase virion attachment, we inoculated TZM-bl cells with X4- and R5-tropic HIV-1 that was pretreated with PAP85-120 fibrils, removed the inoculum 1 h later, and measured the quantity of cell-associated virus by p24 enzyme-linked immunosorbent assay (ELISA). Our res ...
... investigate whether the fibrils increase virion attachment, we inoculated TZM-bl cells with X4- and R5-tropic HIV-1 that was pretreated with PAP85-120 fibrils, removed the inoculum 1 h later, and measured the quantity of cell-associated virus by p24 enzyme-linked immunosorbent assay (ELISA). Our res ...
Approach to the interpretation of endometrial biopsies and curettings
... the normal gland to stroma ratio is maintained or there is slight increase. The endometrium shows proliferative activity, with cystically dilated glands of irregular sizes and shapes. Some glands may exhibit “out‐pouchings”, “infoldings” and “budding”. There is no nuclear atypia, the nuclei being ov ...
... the normal gland to stroma ratio is maintained or there is slight increase. The endometrium shows proliferative activity, with cystically dilated glands of irregular sizes and shapes. Some glands may exhibit “out‐pouchings”, “infoldings” and “budding”. There is no nuclear atypia, the nuclei being ov ...
Detoxification of Arsenic by Phytochelatins in Plants
... (Moench) Garcke cell suspension cultures were cultivated as reported previously (Grill et al., 1987). For experiments, 6 g (fresh weight) of cells were diluted into 30 mL of fresh medium (Linsmaier and Skoog, 1965). Three days after transfer, cells were treated with different concentrations of As or ...
... (Moench) Garcke cell suspension cultures were cultivated as reported previously (Grill et al., 1987). For experiments, 6 g (fresh weight) of cells were diluted into 30 mL of fresh medium (Linsmaier and Skoog, 1965). Three days after transfer, cells were treated with different concentrations of As or ...
Atrophic Vaginitis
... d. Rule out genital herpes with herpes select serology IGG type 2 testing. HSV 2 (herpes simplex virus) may be latent and/or asymptomatic for decades, activating in perimenopause or menopause. Genital HSV 2 is very common, and prevalence increases with age, affecting 1 out of 3 women over 30 years o ...
... d. Rule out genital herpes with herpes select serology IGG type 2 testing. HSV 2 (herpes simplex virus) may be latent and/or asymptomatic for decades, activating in perimenopause or menopause. Genital HSV 2 is very common, and prevalence increases with age, affecting 1 out of 3 women over 30 years o ...
Gram Stain RL.20.02 Michigan Regional Laboratory System
... Take corrective action when stained smear preparations show evidence of poor quality, stains are difficult to interpret, or interpretations are inaccurate. Poor staining characteristics (e.g., faintly staining gram-positive organisms, retention of crystal violet by gram negative organisms, staining ...
... Take corrective action when stained smear preparations show evidence of poor quality, stains are difficult to interpret, or interpretations are inaccurate. Poor staining characteristics (e.g., faintly staining gram-positive organisms, retention of crystal violet by gram negative organisms, staining ...
Abnormal Uterine Bleeding - Intermountain Healthcare
... • It’s complex. AUB has numerous possible causes, and management can involve many decisions about diagnosis and treatment. Changing terminology in the medical literature, narrow recommendations focused on particular age groups or symptoms, and guidelines specific to select AUB causes have done litt ...
... • It’s complex. AUB has numerous possible causes, and management can involve many decisions about diagnosis and treatment. Changing terminology in the medical literature, narrow recommendations focused on particular age groups or symptoms, and guidelines specific to select AUB causes have done litt ...
Challenges in Endometrial biopsy
... arrangement or affiliation with one or more organizations that could be perceived as a direct or indirect conflict of interest in the content of this presentation. ...
... arrangement or affiliation with one or more organizations that could be perceived as a direct or indirect conflict of interest in the content of this presentation. ...
Lab Dept: Microbiology/Virology Test Name: GRAM STAIN
... agents, or failure of organisms to grow under usual culture conditions (medium, atmosphere, etc.). ● Gram stain is not recommended on female genital specimens for Neisseria gonorrhoeae because of lack of specificity. ● Certain organisms do not stain well or at all with Gram stain such as mycobacteri ...
... agents, or failure of organisms to grow under usual culture conditions (medium, atmosphere, etc.). ● Gram stain is not recommended on female genital specimens for Neisseria gonorrhoeae because of lack of specificity. ● Certain organisms do not stain well or at all with Gram stain such as mycobacteri ...
Gram stain reagents - Bakersfield College
... 10. The slide should appear only lightly colored to the naked eye. A good slide is evenly stained and the bacteria are spread thinly enough that you can identify individual cells. The bacteria should not be in clumps as this will alter the amount of stain retained in that area. 11. Observe the slide ...
... 10. The slide should appear only lightly colored to the naked eye. A good slide is evenly stained and the bacteria are spread thinly enough that you can identify individual cells. The bacteria should not be in clumps as this will alter the amount of stain retained in that area. 11. Observe the slide ...
Pokeweed Antiviral Protein, a Ribosome Inactivating Protein: Activity
... Type 3 RIPs are synthesized as inert precursors (pro-RIPs), and undergo proteolytic modifications to acquire their enzymatically activity [23]. These RIPs are much less common than either type 1 or type 2 RIPs. Type 3 RIPs have been identified from maize (Zea mays) and barley (from H. vulgare) [49–5 ...
... Type 3 RIPs are synthesized as inert precursors (pro-RIPs), and undergo proteolytic modifications to acquire their enzymatically activity [23]. These RIPs are much less common than either type 1 or type 2 RIPs. Type 3 RIPs have been identified from maize (Zea mays) and barley (from H. vulgare) [49–5 ...
IN VITRO L. MADRASPATENSIS
... phase consisted of hexane: ethyl acetate (8:2) which gave best resolution of free lupeol at Rf 0.54. Lupeol band observed, confirmed the presence of lupeol in the extract. Phyllanthin was identfiied in methanol extract of P. Maderaspatensis in the mobile phase which consisted of hexane/ acetone/ eth ...
... phase consisted of hexane: ethyl acetate (8:2) which gave best resolution of free lupeol at Rf 0.54. Lupeol band observed, confirmed the presence of lupeol in the extract. Phyllanthin was identfiied in methanol extract of P. Maderaspatensis in the mobile phase which consisted of hexane/ acetone/ eth ...
High performance ion channel screening
... It was designed to automate the standard, two-electrode, voltage clamp screening procedure in a 15 μl bath, covered by a glass plate with a funnel for precise, automated drug application by the liquid handling system. This set-up allows very fast solution changes and requires only minimal amounts of ...
... It was designed to automate the standard, two-electrode, voltage clamp screening procedure in a 15 μl bath, covered by a glass plate with a funnel for precise, automated drug application by the liquid handling system. This set-up allows very fast solution changes and requires only minimal amounts of ...
Development of Combinatorial Peptide Screening to Identify Novel
... including lysine/glutamic acid (KE) and asparagine (N) through rational and biomimetic design1,2. However, rational design only allows access to a (KE)4 limited number of sequences for testing. In order to probe for novel low fouling sequences we have developed a new combinatorial screening method t ...
... including lysine/glutamic acid (KE) and asparagine (N) through rational and biomimetic design1,2. However, rational design only allows access to a (KE)4 limited number of sequences for testing. In order to probe for novel low fouling sequences we have developed a new combinatorial screening method t ...
Poster GIGA DAY Lechanteur
... 1) Selected siRNA anti-E6 and anti-E7 can induce apoptosis and decrease proliferation of HPV16+ cells (by their action against mRNA and proteins). 2) siRNA against anti-apoptotic MCL-1 protein allow higher induction of apoptosis on SiHa cells. The effect of this siRNA must be tested on CaSki cells. ...
... 1) Selected siRNA anti-E6 and anti-E7 can induce apoptosis and decrease proliferation of HPV16+ cells (by their action against mRNA and proteins). 2) siRNA against anti-apoptotic MCL-1 protein allow higher induction of apoptosis on SiHa cells. The effect of this siRNA must be tested on CaSki cells. ...
Gram stain reagents - Bakersfield College
... 10. The slide should appear only lightly colored to the naked eye. A good slide is evenly stained and the bacteria are spread thinly enough that you can identify individual cells. The bacteria should not be in clumps as this will alter the amount of stain retained in that area. 11. Observe the slide ...
... 10. The slide should appear only lightly colored to the naked eye. A good slide is evenly stained and the bacteria are spread thinly enough that you can identify individual cells. The bacteria should not be in clumps as this will alter the amount of stain retained in that area. 11. Observe the slide ...
Cofactor requirement of ribosome-inactivating
... an amino-terminal putative targeting peptide for extracelIn the following discussion, an RIP is considered as lular deposition or intracellular sequestration have been cofactor-dependent when, in the experimental conditions, detected in several other RIPs (references in Irvin and it is over 100-fold ...
... an amino-terminal putative targeting peptide for extracelIn the following discussion, an RIP is considered as lular deposition or intracellular sequestration have been cofactor-dependent when, in the experimental conditions, detected in several other RIPs (references in Irvin and it is over 100-fold ...
Practice Transformation for Cytotechnologists: The Mayo Clinic
... or emerging market gap ..there is economic justification...for a more highly skilled cytology profession Predicts clinician and pathologist shortage...requires new systems ...
... or emerging market gap ..there is economic justification...for a more highly skilled cytology profession Predicts clinician and pathologist shortage...requires new systems ...
Pap test
The Papanicolaou test (abbreviated as Pap test, known earlier as Pap smear, cervical smear, or smear test) is a method of cervical screening used to detect potentially pre-cancerous and cancerous processes in the cervix (opening of the uterus or womb). Abnormal findings are often followed up by more sensitive diagnostic procedures, and, if warranted, interventions that aim to prevent progression to cervical cancer. The test was invented by and named after the prominent Greek doctor Georgios Papanikolaou.A Pap smear is performed by opening the vaginal canal with a speculum, then collecting cells at the outer opening of the cervix at the transformation zone (where the outer squamous cervical cells meet the inner glandular endocervical cells). The collected cells are examined under a microscope to look for abnormalities. The test aims to detect potentially pre-cancerous changes (called cervical intraepithelial neoplasia (CIN) or cervical dysplasia; the squamous intraepithelial lesion system (SIL) is also used to describe abnormalities), which are caused by sexually transmitted human papillomaviruses. The test remains an effective, widely used method for early detection of pre-cancer and cervical cancer. While the test may also detect infections and abnormalities in the endocervix and endometrium, it is not designed to do so.In the United States, Pap smear screening is recommended starting around 21 years of age until the age of 65. Guidelines on frequency vary from every three to five years. If results are abnormal, and depending on the nature of the abnormality, the test may need to be repeated in six to twelve months. If the abnormality requires closer scrutiny, the person may be referred for detailed inspection of the cervix by colposcopy. The person may also be referred for HPV DNA testing, which can serve as an adjunct to Pap testing. Additional biomarkers which may be applied as ancillary tests with the Pap test are evolving.