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LECTURE 5: LINKAGE AND GENETIC MAPPING Reading for this
LECTURE 5: LINKAGE AND GENETIC MAPPING Reading for this

Transposition - Pennsylvania State University
Transposition - Pennsylvania State University

... Transposable elements • Mobile genetic elements - they move from one location in the genome to another • Found in all organisms (so far studied) • Effects: – Insertion near or within a gene can inactivate or activate the target gene. – Cause deletions, inversions, and translocations of DNA – Lead t ...
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... They take pictures of our DNA when it is condensed into the form of chromosomes After that they group the 23 pairs into matched pairs When chromosomes are arranged like this it is called karyotyping (karyotype) ...
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Down Syndrome Research and Practice Volume 5 Issue 3 Pages
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... disturbance of the oxidant-antioxidant system could be the direct cause of this chromosomal nondisjunction. These data as well as the predominant maternal origin of the extra chromosome and the age-dependent incidence was the basis for the mtDNA sequencing in a donor of extra chromosome 21. Three ne ...
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... By contrast, mtDNA is passed on to an individual only from that person’s mother, and it is passed on with (virtually) no change. This point bears emphasizing: all of a person’s mitochondria are derived from his or her mother only – there is ordinarily no paternal contribution [4]. Because of this f ...
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... they are commonly used to multiply or express particular genes. Plasmids used in genetic engineering are called vectors. Vectors are vehicles to transfer genes from one organism to another and typically contain a genetic marker conferring a phenotype. Most also contain a polylinker or multiple cloni ...
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GENETIC ENGINEERING - PLASMIDS, EPISOMES
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... such enzymes, scientists learned to cut specific genes from DNA and to build customized DNA using these genes. They also learned about vectors, strands of DNA such as viruses, which can infect a cell and insert themselves into its DNA. With this knowledge, scientists started to build vectors which i ...
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Chromosome



A chromosome (chromo- + -some) is a packaged and organized structure containing most of the DNA of a living organism. It is not usually found on its own, but rather is complexed with many structural proteins called histones as well as associated transcription (copying of genetic sequences) factors and several other macromolecules. Two ""sister"" chromatids (half a chromosome) join together at a protein junction called a centromere. Chromosomes are normally visible under a light microscope only when the cell is undergoing mitosis. Even then, the full chromosome containing both joined sister chromatids becomes visible only during a sequence of mitosis known as metaphase (when chromosomes align together, attached to the mitotic spindle and prepare to divide). This DNA and its associated proteins and macromolecules is collectively known as chromatin, which is further packaged along with its associated molecules into a discrete structure called a nucleosome. Chromatin is present in most cells, with a few exceptions - erythrocytes for example. Occurring only in the nucleus of eukaryotic cells, chromatin composes the vast majority of all DNA, except for a small amount inherited maternally which is found in mitochondria. In prokaryotic cells, chromatin occurs free-floating in cytoplasm, as these cells lack organelles and a defined nucleus. The main information-carrying macromolecule is a single piece of coiled double-stranded DNA, containing many genes, regulatory elements and other noncoding DNA. The DNA-bound macromolecules are proteins, which serve to package the DNA and control its functions. Chromosomes vary widely between different organisms. Some species such as certain bacteria also contain plasmids or other extrachromosomal DNA. These are circular structures in the cytoplasm which contain cellular DNA and play a role in horizontal gene transfer.Compaction of the duplicated chromosomes during cell division (mitosis or meiosis) results either in a four-arm structure (pictured to the right) if the centromere is located in the middle of the chromosome or a two-arm structure if the centromere is located near one of the ends. Chromosomal recombination during meiosis and subsequent sexual reproduction plays a vital role in genetic diversity. If these structures are manipulated incorrectly, through processes known as chromosomal instability and translocation, the cell may undergo mitotic catastrophe and die, or it may unexpectedly evade apoptosis leading to the progression of cancer.In prokaryotes (see nucleoids) and viruses, the DNA is often densely packed and organized. In the case of archaea by homologs to eukaryotic histones, in the case of bacteria by histone-like proteins. Small circular genomes called plasmids are often found in bacteria and also in mitochondria and chloroplasts, reflecting their bacterial origins.
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