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immuno 2015 - hippocampus
immuno 2015 - hippocampus

... √ Blocking step – 5% donkey normal serum, 1 hour RT drip excess of slides and add 150 uL primary antibody cocktail to the slides Primary antibodies – overnight 4ºC Mouse anti-GFAP binds to Glial fibrillary acidic protein, a marker of astrocytes Rabbit anti-Neurofilament-M recognizes medium chain n ...
Tunnel transitions in the valence band of germanium and inversion
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animal science nutrition laboratory standard procedures and safety

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Nutrition for Life: The food we eat

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Recap - CLASSE Cornell

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Electrooptical properties of metal organic ionic liquid crystals

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... Acid Fast Staining is used primarily for detection of organisms with a thick outer coat composed of true waxes, mycolic acids and phosphatides Mycobacteria are not decolorized and retain the stain, appearing pink under the light microscope (hence are 'fast', like color-fast clothes). Acid Fast Stain ...
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Circular dichroism



Circular dichroism (CD) is dichroism involving circularly polarized light, i.e., the differential absorption of left- and right-handed light. Left-hand circular (LHC) and right-hand circular (RHC) polarized light represent two possible spin angular momentum states for a photon, and so circular dichroism is also referred to as dichroism for spin angular momentum. This phenomenon was discovered by Jean-Baptiste Biot, Augustin Fresnel, and Aimé Cotton in the first half of the 19th century. It is exhibited in the absorption bands of optically active chiral molecules. CD spectroscopy has a wide range of applications in many different fields. Most notably, UV CD is used to investigate the secondary structure of proteins. UV/Vis CD is used to investigate charge-transfer transitions. Near-infrared CD is used to investigate geometric and electronic structure by probing metal d→d transitions. Vibrational circular dichroism, which uses light from the infrared energy region, is used for structural studies of small organic molecules, and most recently proteins and DNA.
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