Axons break in animals lacking β-spectrin
... The proximal end has initiated a new growth cone (open arrowhead). At 48 h, the secondary growth has reached the dorsal cord, albeit by an aberrant posterior route (open arrowheads). (The new dorsal and posterior neuron is DVB [asterisks], which arises postembryonically and sends an axon ventrally t ...
... The proximal end has initiated a new growth cone (open arrowhead). At 48 h, the secondary growth has reached the dorsal cord, albeit by an aberrant posterior route (open arrowheads). (The new dorsal and posterior neuron is DVB [asterisks], which arises postembryonically and sends an axon ventrally t ...
Distinct roles of transcription factors EGL-46 and DAF
... morphology and synaptic contacts (Sulston et al., 1980). Ablation of either HOA or HOB results in a Lov phenotype, indicating that HOA and HOB have non-redundant functions (Liu and Sternberg, 1995). The C. elegans homologues of human autosomal dominant polycystic kidney disease genes PKD1 (lov-1) an ...
... morphology and synaptic contacts (Sulston et al., 1980). Ablation of either HOA or HOB results in a Lov phenotype, indicating that HOA and HOB have non-redundant functions (Liu and Sternberg, 1995). The C. elegans homologues of human autosomal dominant polycystic kidney disease genes PKD1 (lov-1) an ...
HLH-14 is a C. elegans Achaete-Scute protein that
... as heterodimers with members of the E/Daughterless (DA) bHLH family (Cabrera and Alonso, 1991; Johnson et al., 1992; Massari and Murre, 2000). Heterodimer formation is mediated by the helices of the bHLH proteins, while the basic regions are important for binding to DNA sequences with an E-box motif ...
... as heterodimers with members of the E/Daughterless (DA) bHLH family (Cabrera and Alonso, 1991; Johnson et al., 1992; Massari and Murre, 2000). Heterodimer formation is mediated by the helices of the bHLH proteins, while the basic regions are important for binding to DNA sequences with an E-box motif ...
Determinant of Caenorhabditis elegans Growth and
... RIAILs with the Bristol allele at the most significantly linked marker had a mean body length of 619 mm, compared to 578 mm for the RIAILs with the Hawaii allele, indicating that in the linkage mapping experiment the Hawaii allele caused a 7% decrease in mean body length. Just as with lifetime fecun ...
... RIAILs with the Bristol allele at the most significantly linked marker had a mean body length of 619 mm, compared to 578 mm for the RIAILs with the Hawaii allele, indicating that in the linkage mapping experiment the Hawaii allele caused a 7% decrease in mean body length. Just as with lifetime fecun ...
Distinct Isoforms of the RFX Transcription Factor DAF
... McLeod, 1995; Huang et al., 1998; Otsuki et al., 2004), brain development and neuronal functions (Ma et al., 2006; Zhang et al., 2006), and ciliogenesis. Cilia develop as specialized subcellular structures with sensory or motile functions that project off many different cell types. Their structure a ...
... McLeod, 1995; Huang et al., 1998; Otsuki et al., 2004), brain development and neuronal functions (Ma et al., 2006; Zhang et al., 2006), and ciliogenesis. Cilia develop as specialized subcellular structures with sensory or motile functions that project off many different cell types. Their structure a ...
The LIM and POU homeobox genes ttx-3 and unc
... loop (Bertrand and Hobert, 2009) to ensure persistent expression of its target genes. A number of transcription factors have been described in the C. elegans nervous system that display similar broad-ranging effects on the terminal differentiation programs executed by the neurons in which they are e ...
... loop (Bertrand and Hobert, 2009) to ensure persistent expression of its target genes. A number of transcription factors have been described in the C. elegans nervous system that display similar broad-ranging effects on the terminal differentiation programs executed by the neurons in which they are e ...
Hypergravity hinders axonal development of motor neurons
... dendritic arbor development in medial spinal motor neurons in rats that innervate and control muscle (Inglis, Zuckerman & Kalb, 2000). Although altering gravity can affect motor neuron development, the genetic and molecular mechanism of gravity’s effect on neuron development is unknown. The nematode ...
... dendritic arbor development in medial spinal motor neurons in rats that innervate and control muscle (Inglis, Zuckerman & Kalb, 2000). Although altering gravity can affect motor neuron development, the genetic and molecular mechanism of gravity’s effect on neuron development is unknown. The nematode ...
How to get on the right track
... interaction between them that controls microtubule organization and thereby protein sorting into axons and dendrites. Once neurons have completed their last cell cycle, they must migrate to their appropriate location and send out processes to make functional connections with their target cells. A cr ...
... interaction between them that controls microtubule organization and thereby protein sorting into axons and dendrites. Once neurons have completed their last cell cycle, they must migrate to their appropriate location and send out processes to make functional connections with their target cells. A cr ...
Introduction to Neuroglia
... ring and send processes to some synaptic sites in the brain. 6 cells (GLR glia) form gap junctions to both muscle cells and motor neurons. ...
... ring and send processes to some synaptic sites in the brain. 6 cells (GLR glia) form gap junctions to both muscle cells and motor neurons. ...
Combinatorial Marking of Cells and Organelles with Split
... With the color coding provided by the different NZ fluorescent proteins, relatively few (perhaps less than thirty) strains could be used to characterize gene expression in all of the 302 C. elegans neurons (118 classes) ...
... With the color coding provided by the different NZ fluorescent proteins, relatively few (perhaps less than thirty) strains could be used to characterize gene expression in all of the 302 C. elegans neurons (118 classes) ...
Neural circuit rewiring: insights from DD synapse remodeling
... cholinergic neurons.2 The COUP-TF nuclear hormone receptor UNC-55 is expressed in the VD neurons and restricts them from remodeling their synapses.6,7 Ectopic expression of UNC-55 in L1 DD neurons can prevent their remodeling, consistent with a model that lack of UNC-55 in DD neurons enables their a ...
... cholinergic neurons.2 The COUP-TF nuclear hormone receptor UNC-55 is expressed in the VD neurons and restricts them from remodeling their synapses.6,7 Ectopic expression of UNC-55 in L1 DD neurons can prevent their remodeling, consistent with a model that lack of UNC-55 in DD neurons enables their a ...
What is C. elegans? What are its navigational strategies?
... DAG and IP3 are messengers in the process of converting an extra-cellular signal to an electrical response (sensory signal transduction) ...
... DAG and IP3 are messengers in the process of converting an extra-cellular signal to an electrical response (sensory signal transduction) ...
Assisted morphogenesis: glial control of dendrite
... neurons possess a single neurite whose proximal end is postsynaptic, resembling postsynaptic sites on dendrites. In unc-6 mutants this neurite also exhibits severe guidance defects, and fails to navigate toward the CEPsh glia [32]. Thus, glia can contribute to dendrite guidance via the secretion of ...
... neurons possess a single neurite whose proximal end is postsynaptic, resembling postsynaptic sites on dendrites. In unc-6 mutants this neurite also exhibits severe guidance defects, and fails to navigate toward the CEPsh glia [32]. Thus, glia can contribute to dendrite guidance via the secretion of ...
Genetic basis of male sexual behavior
... sexual behaviors related more-or-less directly to the accomplishment of fertilization. We do not include the many social and sexual behaviors expressed by the males of many species that are related to competition with other males, including display, territoriality, and conflict, or male contribution ...
... sexual behaviors related more-or-less directly to the accomplishment of fertilization. We do not include the many social and sexual behaviors expressed by the males of many species that are related to competition with other males, including display, territoriality, and conflict, or male contribution ...
Additional Problem Set 1. The C. elegans gene her
... to Notch that expresses green fluorescence protein (GFP) in both the nervous system (the MP2 lineage) and the mesoderm. The other type is heterozygous for a null Delta mutation, and the chromosome containing the wild-type Delta allele has a transgene linked to Delta that expresses GFP in both the ne ...
... to Notch that expresses green fluorescence protein (GFP) in both the nervous system (the MP2 lineage) and the mesoderm. The other type is heterozygous for a null Delta mutation, and the chromosome containing the wild-type Delta allele has a transgene linked to Delta that expresses GFP in both the ne ...
Potassium channels in C. elegans
... 2.1. Voltage-gated potassium channels Voltage-gated potassium channels are the largest class of 6TM channels. These channels are activated by depolarization, but function with a wide diversity of activation and inactivation kinetics. In mammals, these channels are widely expressed in brain, muscle a ...
... 2.1. Voltage-gated potassium channels Voltage-gated potassium channels are the largest class of 6TM channels. These channels are activated by depolarization, but function with a wide diversity of activation and inactivation kinetics. In mammals, these channels are widely expressed in brain, muscle a ...
Claudia - Phillips Academy
... compared wild type and unc-70 worms when both were exposed to pxn-2 RNAi bacteria, and the second study examined the effect of pxn-2 RNAi bacteria on unc-70 worms alone. Using the GFP filter on the microscope, complete and incomplete commissures were counted in L4 and adult stage C. elegans from eac ...
... compared wild type and unc-70 worms when both were exposed to pxn-2 RNAi bacteria, and the second study examined the effect of pxn-2 RNAi bacteria on unc-70 worms alone. Using the GFP filter on the microscope, complete and incomplete commissures were counted in L4 and adult stage C. elegans from eac ...
The PLAT domain of LOV-1 interacts with ATP-2 to
... The nematode C. elegans is a simple but powerful animal model for studying basic molecular mechanisms underlying human ADPKD. The C. elegans LOV-1 and PKD-2 proteins are homologues of human PC-1 and PC-2 (Barr and Sternberg, 1999; Barr et al., 2001). lov-1 (location of vulva) and pkd-2 act nonredund ...
... The nematode C. elegans is a simple but powerful animal model for studying basic molecular mechanisms underlying human ADPKD. The C. elegans LOV-1 and PKD-2 proteins are homologues of human PC-1 and PC-2 (Barr and Sternberg, 1999; Barr et al., 2001). lov-1 (location of vulva) and pkd-2 act nonredund ...
uncorrected proof - Illinois State University Websites
... shows a high degree of overlap. Here we report that while all larval stages of C. elegans are capable of thermotaxis, aside from adult animals, only L4 and dauer larvae display magnetotactic behavior. Investigating how cilia and villi function during magnetic orientation and transduction in C. elega ...
... shows a high degree of overlap. Here we report that while all larval stages of C. elegans are capable of thermotaxis, aside from adult animals, only L4 and dauer larvae display magnetotactic behavior. Investigating how cilia and villi function during magnetic orientation and transduction in C. elega ...
Newswire Newswire - Rockefeller University
... Cori Bargmann, Torsten N. Wiesel Professor and head of the Lulu and Anthony Wang Laboratory of Neural Circuits and Behavior, has won the 2016 Edward M. Scolnick Prize in Neuroscience, an award given by the McGovern Institute for Brain Research at MIT to recognize outstanding advances in the field. T ...
... Cori Bargmann, Torsten N. Wiesel Professor and head of the Lulu and Anthony Wang Laboratory of Neural Circuits and Behavior, has won the 2016 Edward M. Scolnick Prize in Neuroscience, an award given by the McGovern Institute for Brain Research at MIT to recognize outstanding advances in the field. T ...
highlights - UT Southwestern
... rather like a tool box without a spanner — somewhat lacking in a key piece of kit. Although the first transgenic rat was reported 12 years ago, genetically modifying the rat genome is still far from routine, predominantly because rat embryonic stem cells have not yet been successfully derived or cul ...
... rather like a tool box without a spanner — somewhat lacking in a key piece of kit. Although the first transgenic rat was reported 12 years ago, genetically modifying the rat genome is still far from routine, predominantly because rat embryonic stem cells have not yet been successfully derived or cul ...
Origins of Human Genome Project
... colleagues were working on a physical map of the nematode, Caenorhabditis elegans.16 C. elegans had been identified by Sydney Brenner as a powerful model to apply genetic techniques to study development and behavior of organisms containing differentiated organs, including a primitive nervous system. ...
... colleagues were working on a physical map of the nematode, Caenorhabditis elegans.16 C. elegans had been identified by Sydney Brenner as a powerful model to apply genetic techniques to study development and behavior of organisms containing differentiated organs, including a primitive nervous system. ...
Engineering new synaptic connections in the C. elegans connectome
... ultimately affecting behavior. For this reason a tremendous research effort is currently being made to obtain detailed connectomes, whole brain synaptic connectivity maps, of various organisms, including humans.1-3 This formidable endeavor follows the earlier, relatively more modest project of mappi ...
... ultimately affecting behavior. For this reason a tremendous research effort is currently being made to obtain detailed connectomes, whole brain synaptic connectivity maps, of various organisms, including humans.1-3 This formidable endeavor follows the earlier, relatively more modest project of mappi ...
UNC-55, an Orphan Nuclear Hormone Receptor, Orchestrates
... RT-PCR . Large numbers of mixed stage animals were grown in liquid culture in a fermentor (Bioflo III, New Brunswick Scientific). Total RNA was isolated using Trizol reagent from Life Technologies (Gaithersburg, MD) following the included protocol. mRNA was isolated from total RNA using an Oligotex ...
... RT-PCR . Large numbers of mixed stage animals were grown in liquid culture in a fermentor (Bioflo III, New Brunswick Scientific). Total RNA was isolated using Trizol reagent from Life Technologies (Gaithersburg, MD) following the included protocol. mRNA was isolated from total RNA using an Oligotex ...
Bioscreening, Biophotonics, and Micromanipulation
... The speed of the sorter depends on the actuation speed of the valves, the concentration of animals at the input, the flow speed of the worms, and the image acquisition and processing times. The technique of immobilizing worms by lowering pressure in a micro-channel is fast because the actuation spee ...
... The speed of the sorter depends on the actuation speed of the valves, the concentration of animals at the input, the flow speed of the worms, and the image acquisition and processing times. The technique of immobilizing worms by lowering pressure in a micro-channel is fast because the actuation spee ...
Caenorhabditis elegans
Caenorhabditis elegans /ˌseɪnɵræbˈdɪtɪs ˈɛlɛɡænz/ is a free-living (not parasitic), transparent nematode (roundworm), about 1 mm in length, that lives in temperate soil environments. The name is a blend of Greek (caeno- - recent, rhabditis - rod-like) and Latin (elegans - elegant). In 1900, Maupas initially named it Rhabditides elegans, Osche placed it in the subgenus Caenorhabditis in 1952, and in 1955, Dougherty raised it to the status of genus.C. elegans is an unsegmented pseudocoelomate, and lacks a respiratory and a circulatory system. They possess gut granules which emit a brilliant blue fluorescence, a wave of which is seen at death in a 'death fluorescence'. The majority of these nematodes are female hermaphrodites. Males have specialised tails for mating that include spicules.In 1963, Sydney Brenner proposed research into C. elegans primarily in the area of neuronal development. In 1974, he began research into the molecular and developmental biology of C. elegans, which has since been extensively used as a model organism.C. elegans was the first multicellular organism to have its whole genome sequenced, and as of 2012, the only organism to have its connectome (neuronal ""wiring diagram"") completed.