Basics in Confocal Microscopy Handouts
... In Total Internal Reflection (TIRF) microscopy light is coupled into the optics above a critical angle which reflects the light totally but creates an evanescent wave about 50-200 nm next to the reflecting surface (cover slip). ...
... In Total Internal Reflection (TIRF) microscopy light is coupled into the optics above a critical angle which reflects the light totally but creates an evanescent wave about 50-200 nm next to the reflecting surface (cover slip). ...
Microscopy Basics
... Bright field microscopy is based on absorption of light in the sample. Most biological objects, however, absorb only weakly in the visible spectrum. This lead to: • Development of specific staining (nowadays almost entirely replaced by fluorescent labeling) • Development of UV microscopy (Köhler) fa ...
... Bright field microscopy is based on absorption of light in the sample. Most biological objects, however, absorb only weakly in the visible spectrum. This lead to: • Development of specific staining (nowadays almost entirely replaced by fluorescent labeling) • Development of UV microscopy (Köhler) fa ...
Chapter 3 – Observing microorganisms through a microscope Units
... o How _______________ do two points have to be in order to see ________________? o Resolving power of 4 μm = two points have to be at least 4 μm apart in order to see them as two points. Two points less than 4 μm apart will look like ______________. Light microscopy - Any kind of microscope that use ...
... o How _______________ do two points have to be in order to see ________________? o Resolving power of 4 μm = two points have to be at least 4 μm apart in order to see them as two points. Two points less than 4 μm apart will look like ______________. Light microscopy - Any kind of microscope that use ...
Nanoscopy with focused light
... Throughout the 20th century it was widely accepted that a light microscope relying on conventional optical lenses cannot discern details that are much finer than about half the wavelength of light (200-400 nm), due to diffraction. However, in the 1990s, the viability to overcome the diffraction barr ...
... Throughout the 20th century it was widely accepted that a light microscope relying on conventional optical lenses cannot discern details that are much finer than about half the wavelength of light (200-400 nm), due to diffraction. However, in the 1990s, the viability to overcome the diffraction barr ...
Microscopy
Microscopy is the technical field of using microscopes to view objects and areas of objects that cannot be seen with the naked eye (objects that are not within the resolution range of the normal eye). There are three well-known branches of microscopy: optical, electron, and scanning probe microscopy.Optical and electron microscopy involve the diffraction, reflection, or refraction of electromagnetic radiation/electron beams interacting with the specimen, and the collection of the scattered radiation or another signal in order to create an image. This process may be carried out by wide-field irradiation of the sample (for example standard light microscopy and transmission electron microscopy) or by scanning of a fine beam over the sample (for example confocal laser scanning microscopy and scanning electron microscopy). Scanning probe microscopy involves the interaction of a scanning probe with the surface of the object of interest. The development of microscopy revolutionized biology and remains an essential technique in the life and physical sciences.