Confocal Microscopy
... GFP and YFP (Distance of emission peaks ca. 12nm) A431 cells expressing GFP, Rab11-YFP ...
... GFP and YFP (Distance of emission peaks ca. 12nm) A431 cells expressing GFP, Rab11-YFP ...
Module P6.4 Optical instruments
... o that lenses and mirrors have perfectly formed spherical surfaces, o that all objects lie very close to the optical axis, o that all rays involved make small angles to this axis (i.e. are paraxial rays), o that all lenses are thin, so each lens produces the same point image of a point object for al ...
... o that lenses and mirrors have perfectly formed spherical surfaces, o that all objects lie very close to the optical axis, o that all rays involved make small angles to this axis (i.e. are paraxial rays), o that all lenses are thin, so each lens produces the same point image of a point object for al ...
In this lab you will use the phenomenon of interference... thickness of thin films. Two interference techniques, Michelson and... Thin Film Measurement 1 Introduction
... polarize the light emitted by the source. The compensator, is a birefringent plate usually of quarterwave thickness. It is used to convert the linearly polarized light into elliptically polarized light. Hence, the light incident upon the sample has an azimuthal angle psi and an ellipticity predictab ...
... polarize the light emitted by the source. The compensator, is a birefringent plate usually of quarterwave thickness. It is used to convert the linearly polarized light into elliptically polarized light. Hence, the light incident upon the sample has an azimuthal angle psi and an ellipticity predictab ...
Microscopes - OpenStax CNX
... Both the objective and the eyepiece contribute to the overall magni cation, which is large and negative, consistent with Figure 2, where the image is seen to be large and inverted. In this case, the image is virtual and inverted, which cannot happen for a single element (case 2 and case 3 images for ...
... Both the objective and the eyepiece contribute to the overall magni cation, which is large and negative, consistent with Figure 2, where the image is seen to be large and inverted. In this case, the image is virtual and inverted, which cannot happen for a single element (case 2 and case 3 images for ...
Microscopy Overview
... Abbe showed that in order to form a perfect lateral image the objective must satisfy the sine condition. Otherwise the image of an off-axis point will suffer from coma. An aberration-free system satisfying the sine condition is called an aplanatic system. A microscope produces a three-dimensional (3 ...
... Abbe showed that in order to form a perfect lateral image the objective must satisfy the sine condition. Otherwise the image of an off-axis point will suffer from coma. An aberration-free system satisfying the sine condition is called an aplanatic system. A microscope produces a three-dimensional (3 ...
Presentation
... confine the NA of the input beam completely or when the proportions of rib do not produce good mode confinement • The loss due to scratches, digs, and chips on the wave guide ends ...
... confine the NA of the input beam completely or when the proportions of rib do not produce good mode confinement • The loss due to scratches, digs, and chips on the wave guide ends ...
Negative Refraction Makes a Perfect Lens
... Optical lenses have for centuries been one of scientists’ prime tools. Their operation is well understood on the basis of classical optics: curved surfaces focus light by virtue of the refractive index contrast. Equally their limitations are dictated by wave optics: no lens can focus light onto an a ...
... Optical lenses have for centuries been one of scientists’ prime tools. Their operation is well understood on the basis of classical optics: curved surfaces focus light by virtue of the refractive index contrast. Equally their limitations are dictated by wave optics: no lens can focus light onto an a ...
Negative Refraction Makes a Perfect Lens
... Optical lenses have for centuries been one of scientists’ prime tools. Their operation is well understood on the basis of classical optics: curved surfaces focus light by virtue of the refractive index contrast. Equally their limitations are dictated by wave optics: no lens can focus light onto an a ...
... Optical lenses have for centuries been one of scientists’ prime tools. Their operation is well understood on the basis of classical optics: curved surfaces focus light by virtue of the refractive index contrast. Equally their limitations are dictated by wave optics: no lens can focus light onto an a ...
Étendue and spectral resolution
... The finesse is a measure of the spectral resolution that can be achieved with an interferometer for a specified FSR – which is determined by experimental requirements. Ideally the interferometer plates should be illuminated by parallel rays of light and these rays should be in the wedge plane of the ...
... The finesse is a measure of the spectral resolution that can be achieved with an interferometer for a specified FSR – which is determined by experimental requirements. Ideally the interferometer plates should be illuminated by parallel rays of light and these rays should be in the wedge plane of the ...
Noise propagation in wave-front sensing with
... 共or active兲 optics systems. For these applications many wave-front sensing techniques have been developed and characterized.1 However, only a few techniques can be used with widely extended sources.2–5 Among them, phase diversity, which requires only two focal-plane images, presents some interesting ...
... 共or active兲 optics systems. For these applications many wave-front sensing techniques have been developed and characterized.1 However, only a few techniques can be used with widely extended sources.2–5 Among them, phase diversity, which requires only two focal-plane images, presents some interesting ...
Wave aberration of human eyes and new descriptors
... begins to increase the blur circle and the effect of diffraction.11 To the contrary, the magnitude of aberrations increases with pupil dilation,12,13 leading to a decrease in visual performance and optical quality of the retinal image. Regarding the third component, controlled changes in the shape a ...
... begins to increase the blur circle and the effect of diffraction.11 To the contrary, the magnitude of aberrations increases with pupil dilation,12,13 leading to a decrease in visual performance and optical quality of the retinal image. Regarding the third component, controlled changes in the shape a ...
(等倾干涉) — equal thickness interference.
... A thin film with index of refraction n = 1.40 is placed in one arm of a Michelson interferometer, perpendicular to the optical path. If this causes a shift of 7.0 fringes of the pattern produced by light of = 589 nm, what is the film thickness? Solution: The change of optical path difference due ...
... A thin film with index of refraction n = 1.40 is placed in one arm of a Michelson interferometer, perpendicular to the optical path. If this causes a shift of 7.0 fringes of the pattern produced by light of = 589 nm, what is the film thickness? Solution: The change of optical path difference due ...
APPENDIX When designing shape magnification into a lens, the two
... When designing shape magnification into a lens, the two main parameters that can be manipulated are the base curve and center thickness. High index lenses are also important considerations as they allow ...
... When designing shape magnification into a lens, the two main parameters that can be manipulated are the base curve and center thickness. High index lenses are also important considerations as they allow ...
Optical aberration
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An optical aberration is a departure of the performance of an optical system from the predictions of paraxial optics. In an imaging system, it occurs when light from one point of an object does not converge into (or does not diverge from) a single point after transmission through the system. Aberrations occur because the simple paraxial theory is not a completely accurate model of the effect of an optical system on light, rather than due to flaws in the optical elements.Aberration leads to blurring of the image produced by an image-forming optical system. Makers of optical instruments need to correct optical systems to compensate for aberration.The articles on reflection, refraction and caustics discuss the general features of reflected and refracted rays.