Relationship between neurotransmitter release and cytosolic free
... On the contrary, PMA, OAG and pHMBS (Table 1) release large amounts of [3H]dopamine without requiring a rise in [Ca2+],,and morphological evidence indicates that with these drugs the release is due to exocytosis: the density of secretory granules is decreased in parallel with the release of [3H]dopa ...
... On the contrary, PMA, OAG and pHMBS (Table 1) release large amounts of [3H]dopamine without requiring a rise in [Ca2+],,and morphological evidence indicates that with these drugs the release is due to exocytosis: the density of secretory granules is decreased in parallel with the release of [3H]dopa ...
Journal of Applied Microbiology
... Methods and Results: Two Aer. veronii mucin- and lactoferrin-binding proteins with molecular masses of 37 and 48 kDa were identified by Western blot analysis. According to its N-terminal amino acid sequence, the 48-kDa protein was identified as Omp48, an outer-membrane protein similar to LamB of Esc ...
... Methods and Results: Two Aer. veronii mucin- and lactoferrin-binding proteins with molecular masses of 37 and 48 kDa were identified by Western blot analysis. According to its N-terminal amino acid sequence, the 48-kDa protein was identified as Omp48, an outer-membrane protein similar to LamB of Esc ...
FEMS Microbiology Ecology 24
... The CSC of three strains, A. brasilense Cd, A. brasilense Sp-245, and A. lipoferum 1842 grown on solid and liquid NB media, was evaluated following different treatments. These treatments were to evaluate whether external treatment of the cells (as those that may occur in the soil) can change the CSC ...
... The CSC of three strains, A. brasilense Cd, A. brasilense Sp-245, and A. lipoferum 1842 grown on solid and liquid NB media, was evaluated following different treatments. These treatments were to evaluate whether external treatment of the cells (as those that may occur in the soil) can change the CSC ...
- Wiley Online Library
... function of hrp (HR and pathogenicity) and hrc (hrp conserved) genes that encode a type III secretion system (TTSS) (Alfano and Collmer, 1997; Lindgren, 1997; He, 1998; Jin et al., 2003). Genes encoding the TTSS have been found in many Gram-negative bacteria including animal pathogens of the genera ...
... function of hrp (HR and pathogenicity) and hrc (hrp conserved) genes that encode a type III secretion system (TTSS) (Alfano and Collmer, 1997; Lindgren, 1997; He, 1998; Jin et al., 2003). Genes encoding the TTSS have been found in many Gram-negative bacteria including animal pathogens of the genera ...
05 Keim
... the cytoplasm, the cell wall, and the periplasm, which is the space between the cell and outer membranes that contains the peptidoglycan layer [5]. Additionally, several prokaryotes form specialized intracellular inclusions [8,35], some of which are restricted to specific bacterial groups and thus a ...
... the cytoplasm, the cell wall, and the periplasm, which is the space between the cell and outer membranes that contains the peptidoglycan layer [5]. Additionally, several prokaryotes form specialized intracellular inclusions [8,35], some of which are restricted to specific bacterial groups and thus a ...
The polar flagellum mediates Azuspidlum brasifense
... flagella. The bacteria and bacterial cell debris were removed from the mixed cell suspension by centri€ugation for 15 min at 12000 r.p.m. From the supernatant liquid, flagella were pelletcd by ultracentrifugation for 90 min at 22000 r.p.m., and resuspended in 1 ml of sterile water. This suspension w ...
... flagella. The bacteria and bacterial cell debris were removed from the mixed cell suspension by centri€ugation for 15 min at 12000 r.p.m. From the supernatant liquid, flagella were pelletcd by ultracentrifugation for 90 min at 22000 r.p.m., and resuspended in 1 ml of sterile water. This suspension w ...
What Does the Microsporidian E. cuniculi Tell Us About the Origin of
... approaching the size of a minimal eukaryotic cell. To understand the differences between minimal cells of Eukarya and Bacteria, we need to characterize the set of proteins in E. cuniculi that is unique to the eukaryotes but absent from other cellular domains. Previously, we collected a set of protein ...
... approaching the size of a minimal eukaryotic cell. To understand the differences between minimal cells of Eukarya and Bacteria, we need to characterize the set of proteins in E. cuniculi that is unique to the eukaryotes but absent from other cellular domains. Previously, we collected a set of protein ...
Growth and killing of a Salmonella enterica serovar
... Ultracut ultramicrotome at 60 nm using a Diatome knife, contrasted with uranyl acetate and lead citrate, and examined in a Philips CM100 transmission electron microscope. For immuno-electron microscopy, HeLa cells were infected with either wild-type or sifA mutant strains. At 10 h after bacterial en ...
... Ultracut ultramicrotome at 60 nm using a Diatome knife, contrasted with uranyl acetate and lead citrate, and examined in a Philips CM100 transmission electron microscope. For immuno-electron microscopy, HeLa cells were infected with either wild-type or sifA mutant strains. At 10 h after bacterial en ...
- Wiley Online Library
... (Kowarz et al., 1994). Salmonella of strain SJW 880 flaR 1656 H1-gt H2-enx, nonmotile nonflagellated mutant strain, were obtained from S. Kato (Nagoya University, Japan). Bacteria were grown in Luria–Bertani broth without NaCl and then washed twice using physiological solution and centrifuged at 200 ...
... (Kowarz et al., 1994). Salmonella of strain SJW 880 flaR 1656 H1-gt H2-enx, nonmotile nonflagellated mutant strain, were obtained from S. Kato (Nagoya University, Japan). Bacteria were grown in Luria–Bertani broth without NaCl and then washed twice using physiological solution and centrifuged at 200 ...
Lethal protein produced in response to competition
... 32 and 30 kDa proteins have been annotated in the P. dendritiformis genome as flagellin (GenBank accession no. GQ891985) and subtilisin Carlsberg (GenBank accession no. GQ891986), respectively. The 12 kDa protein corresponded to a gene predicted to encode a larger, 173 amino acid, protein. This prot ...
... 32 and 30 kDa proteins have been annotated in the P. dendritiformis genome as flagellin (GenBank accession no. GQ891985) and subtilisin Carlsberg (GenBank accession no. GQ891986), respectively. The 12 kDa protein corresponded to a gene predicted to encode a larger, 173 amino acid, protein. This prot ...
Production of Outer Membrane Vesicles in a Clinical
... other secretion systems, OMVs may be a means by which some soluble proteins and other compounds susceptible to enzymatic decomposition, such as toxins and nucleic acids, may be encapsulated to be released in a protective structure. The latter allows them to reach the destination without any alterati ...
... other secretion systems, OMVs may be a means by which some soluble proteins and other compounds susceptible to enzymatic decomposition, such as toxins and nucleic acids, may be encapsulated to be released in a protective structure. The latter allows them to reach the destination without any alterati ...
Binding Sites for Bacterial Flagella at the Surface of the Soil Amoeba
... Following postfixation in OsO,, samples to be processed for transmission electron microscopy were immersed in 0.5% (w/v) aqueous uranyl acetate for 30 min before dehydration through ethanol/water mixtures. After three changes in absolute ethanol the specimens were transferred via a 1 :1 acetone/Aral ...
... Following postfixation in OsO,, samples to be processed for transmission electron microscopy were immersed in 0.5% (w/v) aqueous uranyl acetate for 30 min before dehydration through ethanol/water mixtures. After three changes in absolute ethanol the specimens were transferred via a 1 :1 acetone/Aral ...
A Simplified Guide to Bacteria in Water Part 1 By D
... At the moment, there is an argument as to which method is better, since both affect the ability of coliforms to grow during the test. The MF method has been reported to sometimes give false negatives since the coliforms become damaged by chlorination, while the MPN test sometimes gives false results ...
... At the moment, there is an argument as to which method is better, since both affect the ability of coliforms to grow during the test. The MF method has been reported to sometimes give false negatives since the coliforms become damaged by chlorination, while the MPN test sometimes gives false results ...
Culturing marine bacteria – an essential - Archimer
... Using multi-locus sequence analysis and a detailed statistical analysis of nine loci for each of 23 isolates, they showed that it was possible to identify different clades within the single species. In general, strains clustered with the depth in the water column from which the isolate originated. T ...
... Using multi-locus sequence analysis and a detailed statistical analysis of nine loci for each of 23 isolates, they showed that it was possible to identify different clades within the single species. In general, strains clustered with the depth in the water column from which the isolate originated. T ...
Group-specific effects on coastal bacterioplankton of
... *Corresponding author. Email: [email protected] ...
... *Corresponding author. Email: [email protected] ...
Journal of Plant Growth Regulation
... the biotic world was classified into two main divisions, plants and animals, on the basis of the conspicuous differences in their anatomy, morphology, behavior, and ecology. In the twentieth century, with ever increasingly sophisticated tools of analysis, cellular-level similarities between these tw ...
... the biotic world was classified into two main divisions, plants and animals, on the basis of the conspicuous differences in their anatomy, morphology, behavior, and ecology. In the twentieth century, with ever increasingly sophisticated tools of analysis, cellular-level similarities between these tw ...
Staphylococcal phenomics: metabolomic and proteomic responses
... Bacteria can be exposed to considerable variations in nutrient and ion concentrations that lead to changes in the external osmotic pressure which demand rapid responses in the ultrastructure and biochemistry of the bacterial cells. A number of mechanisms have evolved in bacteria to survive osmotic c ...
... Bacteria can be exposed to considerable variations in nutrient and ion concentrations that lead to changes in the external osmotic pressure which demand rapid responses in the ultrastructure and biochemistry of the bacterial cells. A number of mechanisms have evolved in bacteria to survive osmotic c ...
Annals of Microbiology
... by TEM after PA-TCH-SP staining (Fig. 3D) showed electrondense exudate outside the root hair wall and bacteria grouped within the curled root hair tip. Furthermore, each bacterium was surrounded by a thick electrontransparent envelope. These features suggest that immediately after their penetration ...
... by TEM after PA-TCH-SP staining (Fig. 3D) showed electrondense exudate outside the root hair wall and bacteria grouped within the curled root hair tip. Furthermore, each bacterium was surrounded by a thick electrontransparent envelope. These features suggest that immediately after their penetration ...
Challenges to our current view on chloroplasts
... small cells like bacteria do not need structural elements, and they appeared as ‘plasma-filled tiny droplets’, although microbiologists were well aware of the different cell shapes bacteria may encounter. Finally, molecular biology on one hand and modern fluorescence microscopic methods on the other ...
... small cells like bacteria do not need structural elements, and they appeared as ‘plasma-filled tiny droplets’, although microbiologists were well aware of the different cell shapes bacteria may encounter. Finally, molecular biology on one hand and modern fluorescence microscopic methods on the other ...
19-1 Bacteria
... Antibiotics work by breaking down the cell wall of gram positive bacteria. Gram negative one are not as affected as their cell wall, so it is important to know what type of bacterial infection one has. ...
... Antibiotics work by breaking down the cell wall of gram positive bacteria. Gram negative one are not as affected as their cell wall, so it is important to know what type of bacterial infection one has. ...
Brachial Plexus block 2 of 2
... the "chimney" effect as local anesthetic is forced to spread up between the anterior and middle scalene muscles, unable to go down because the first rib is in the way. ...
... the "chimney" effect as local anesthetic is forced to spread up between the anterior and middle scalene muscles, unable to go down because the first rib is in the way. ...
SPI-1 encoded genes ofSalmonellaTyphimurium influence
... Typhimurium) is a facultative, intracellular pathogen capable of causing severe gastroenteritis in mammals including humans. Although the virulence of Salmonella Typhimurium is a multifactorial phenotype, there are two key virulence determinants specific for S. enterica. To successfully interact wit ...
... Typhimurium) is a facultative, intracellular pathogen capable of causing severe gastroenteritis in mammals including humans. Although the virulence of Salmonella Typhimurium is a multifactorial phenotype, there are two key virulence determinants specific for S. enterica. To successfully interact wit ...
isolation of a bacteriophage from sewage sludge
... Figure 1: Basic Structure of Bacteriophage (Madigan and Martinko, 2006). Enteric bacteria are normal inhabitants of the intestines of humans and other animals (Davis, 2005) but are often isolated from aquatic ecosystems after sewage has been introduced into the environment. Sewage contains high numb ...
... Figure 1: Basic Structure of Bacteriophage (Madigan and Martinko, 2006). Enteric bacteria are normal inhabitants of the intestines of humans and other animals (Davis, 2005) but are often isolated from aquatic ecosystems after sewage has been introduced into the environment. Sewage contains high numb ...
magnetosome formation in prokaryotes
... Dennis A. Bazylinski* and Richard B. Frankel‡ Magnetotactic bacteria were discovered almost 30 years ago, and for many years and many different reasons, the number of researchers working in this field was few and progress was slow. Recently, however, thanks to the isolation of new strains and the de ...
... Dennis A. Bazylinski* and Richard B. Frankel‡ Magnetotactic bacteria were discovered almost 30 years ago, and for many years and many different reasons, the number of researchers working in this field was few and progress was slow. Recently, however, thanks to the isolation of new strains and the de ...
Type three secretion system
Type three secretion system (often written Type III secretion system and abbreviated TTSS or T3SS, also called Injectisome or Injectosome) is a protein appendage found in several Gram-negative bacteria.In pathogenic bacteria, the needle-like structure is used as a sensory probe to detect the presence of eukaryotic organisms and secrete proteins that help the bacteria infect them. The secreted effector proteins are secreted directly from the bacterial cell into the eukaryotic (host) cell, where they exert a number of effects that help the pathogen to survive and to escape an immune response.