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Get PDF - OSA Publishing
Get PDF - OSA Publishing

... function (PSF) of high numerical aperture (NA) systems in imaging and focusing applications. A planar refractive index mismatch is introduced by a suspension medium, a glass cover slide or imaging into a thick transparent specimen, thereby inducing a focus shift and spherical aberrations. This reduc ...
Advances in diamond-turned surfaces enable unique cost
Advances in diamond-turned surfaces enable unique cost

... imaging hyperspectral optical systems. These processes have been developed for applications in the short-wave visible through long-wave IR wavelengths. Optical designs for these imaging systems are typically Offner or Dyson configurations, where the critical optical components are powered gratings a ...
In the diagram below, the optical train of a set of binoculars is found
In the diagram below, the optical train of a set of binoculars is found

Interferometric back focal plane microellipsometry
Interferometric back focal plane microellipsometry

... inequality holds, h would need to be known ~at least roughly! beforehand. This condition is readily met for dielectric materials for which k is very small @the case where Eq. ~12b! is required# and h is typically not smaller than 1.5. In summary, by measuring d, we obtain f0, and by measuring R0 fur ...
The present work gives recommendations for rational - Dimka
The present work gives recommendations for rational - Dimka

Lenserf Reflection, Fresnel Reflection,
Lenserf Reflection, Fresnel Reflection,

... all the power or energy in the incident wave is now totally reflected back into the specularly reflected wave in the lower region. Without getting entangled in detailed mathematics, we can note that, in this TIR regime, the evanescent refracted wave becomes what is commonly called an inhomogeneous pla ...
Optical properties of Al2O3 thin films grown by
Optical properties of Al2O3 thin films grown by

... Al2 O3 films on soda lime glass. For the theoretical calculation, the effective refractive index [23] for different modes was calculated for all three samples and the refractive index and film thickness were taken from the fitting results. For experimental verification, the prism coupling method [24 ...
Ultrasonic nondestructive characterization of metallurgical reactions
Ultrasonic nondestructive characterization of metallurgical reactions

The Effect of Phase Change on Reflection on Optical Measurements
The Effect of Phase Change on Reflection on Optical Measurements

... A major source of uncertainty is the background subtraction. The pre- and post-deposition samples can not be repositioned exactly in the same orientation in the interferometer. Rotation, translation, and zoom differences occur between the two measurements. The trimming of the edge pixels also contri ...
Unit one: Periodic Motion Lesson 1: Oscillatory Motion. Lesson 2
Unit one: Periodic Motion Lesson 1: Oscillatory Motion. Lesson 2

... Question (4) on the next graph papers, represent the oscillating motion for: 1- Pendulum with max. Displacement = 3 cm making one complete oscillation. 2- Pendulum with max. Displacement = 4 cm making half oscillation. ...
Chapter 12: Light
Chapter 12: Light

... Section 2: Light and Color Color and Wavelength • The colors of the visible spectrum differ from one another in terms of wavelength • Each combination of wavelengths has its own special sense of color • When white light strikes most objects certain colors are absorbed and certain colors are reflect ...
Light - Webs
Light - Webs

... • We also said that the speed of a wave in a certain medium is always constant. • It has been found that the speed of EM waves and light is --– 3 x 108 or 300,000,000 m/s – 671,000,000 mph – 186,000 miles per second – We call this value “c” © 2003 Mike Maloney ...
UV-light microscope: improvements in optical imaging for a
UV-light microscope: improvements in optical imaging for a

... system. The original color camera operated at lower resolution with an effective pixel size corresponding to 1.2 mm on the sample surface. The new higher resolution monochrome camera in the UV-light microscope system has an effective pixel size measuring 0.3–0.4 mm on the sample surface, so that the ...
Reflection of a Ray of Light Introduction: Purpose
Reflection of a Ray of Light Introduction: Purpose

Polarization
Polarization

... analyzer) is rotated by hand, the relative light intensity is recorded as a function of the angle between the axes of polarization of the two polarizers. The angle is obtained using a Rotary Motion Sensor that is coupled to the polarizer with a drive belt. The plot of light intensity versus angle ca ...
Fiber Bragg Gratings
Fiber Bragg Gratings

... the material science and fabrication behind FBGs. Several methods have been developed in the late 20th century to accurately describe and model electromagnetic wave propagation across periodic structures. The most popular approach is coupled mode theory (ref), in which the coupling between the eigen ...
Waves, Photons and Medical Physics
Waves, Photons and Medical Physics

... to the open end of a resonance tube closed at the opposite end. The frequency of the signal generator is adjusted so that the first two positions of resonance are obtained. On Fig. 4.1 and Fig. 4.2 draw representations of the modes of vibration at these positions of resonance, and label all nodes (N ...
Introduction to Nonlinear Optics
Introduction to Nonlinear Optics

... |A2(L)|2 = 2 2 [ (2)]2 |A1|4L2 sinc2( k L/2) ...
Controllable Optical Phase Shift Over One Radian from a Single
Controllable Optical Phase Shift Over One Radian from a Single

... and spot diameter of 5 µm (full-width at half-maximum). The illumination beam is linearly polarised to eliminate optical pumping effects and its power is actively stabilised to minimise intensity fluctuations between reference and signal images. The light transmitted past the atom is reimaged onto a ...
Backward wave propagation in left-handed media with isotropic and
Backward wave propagation in left-handed media with isotropic and

... in the plane containing both the optical axis and the propagation direction, then the wave is considered extraordinary. The k-space diagram for the extraordinary wave propagating through a medium with material parameters ⑀s = 1, ⑀z = −2, and ␮r = 1 is shown in Fig. 2. It should be noted that since i ...
High‐transmission dielectric metasurface with 2π phase control at
High‐transmission dielectric metasurface with 2π phase control at

... transmission into the desired diffraction order. The maximum transmission is limited by material loss and can further be improved, e.g. by using other materials with lower losses in the visible (e.g TiO2 , GaN, etc.). It is known [19, 20, 28–32] that in spherical high refractive index dielectric par ...
Nanofabrication via Atom Optics with Chromium
Nanofabrication via Atom Optics with Chromium

... distribution of the transversely-laser-cooled atoms. Its presence could in principle interfere with etching of the substrate in the spaces between the lines. We have found, however, that the background of chromium between the lines is not a serious impediment to etching highly regular patterns into ...
untitled - PhysRevLett.111.243901
untitled - PhysRevLett.111.243901

... manifested, however, if it stays coherent during propagation and the structural variations of the medium are negligible over the transit time. Then it is possible to deterministically control the light propagation through disordered media, which even enables us to use the media as useful optical ele ...
Inter 1-4
Inter 1-4

... dark fringes is known as fringe width and is same everywhere on the screen. For bright fringes ; mth order fringe occurs when ...
Chapter 3: prisms
Chapter 3: prisms

< 1 ... 67 68 69 70 71 72 73 74 75 ... 119 >

Surface plasmon resonance microscopy



Surface Plasmon Resonance Microscopy (SPRM) is a label free analytical tool that combines the surface plasmon resonance of metallic surfaces with imaging of the metallic surface.The heterogeneity of the refractive index of the metallic surface imparts high contrast images, caused by the shift in the resonance angle.SPRM can achieve a thickness sensitivity of few tenths of nanometer and lateral resolution achieves values of micrometer scale.SPRM is used to characterize surfaces, self-assembled monolayers, multilayer films, metal nanoparticles, oligonucleotides arrays, binding and reduction reactions.Surface Plasmon polaritons are surface electromagnetic waves coupled to oscillating free electrons of a metallic surface that propagate along a metal/dielectric interface.Since polaritons are highly sensitive to small changes in the refractive index of the metallic material,it can be used as a biosensing tool that does not require labeling. SPRM measurements can be made in real-time.Wang and collaborators studied the binding kinetics of membrane proteins in single cells.The experimental setup of an SPRM can be seen in the Figure 1, where an adherent cell is grown on a gold film and placed in an inverted microscope, p-polarized light was used to create the surface plasmons on the gold film and a CCD camera was used to create the SPR image.
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