Protein C-mannosylation: Facts and questions.
... occurring in ER. Based on this, the hypothesis was ventured that the precursor in the biosynthesis of (C2-Man)-Trp is dolichylphosphate mannose (Dol-P-Man). This was verified by expression of RNase 2.4 in CHO Lec15 cells, in which the Dol-P-Man synthase activity is very low and which contain strongl ...
... occurring in ER. Based on this, the hypothesis was ventured that the precursor in the biosynthesis of (C2-Man)-Trp is dolichylphosphate mannose (Dol-P-Man). This was verified by expression of RNase 2.4 in CHO Lec15 cells, in which the Dol-P-Man synthase activity is very low and which contain strongl ...
Amino Acids, Peptides and Proteins
... For a dodecapeptide (20 aa) it requires 40 chemical steps (not counting special treatment for some side groups). If the yield is 90% per step the overall yield is only (0.940) 1.5% If the yield is 99% per step the overall yield is only (0.9940) 67% If the yield is 99.9% per step the overall yield is ...
... For a dodecapeptide (20 aa) it requires 40 chemical steps (not counting special treatment for some side groups). If the yield is 90% per step the overall yield is only (0.940) 1.5% If the yield is 99% per step the overall yield is only (0.9940) 67% If the yield is 99.9% per step the overall yield is ...
Ribosomal Protein S7 Distinguishes Multicellular from Unicellular
... only copies of Rps S7 found in multicelllular fungi are not those found in mitochondria. Of the ribosomal proteins of the S subunit that we have studied in detail this uncommon property of S7 is shared by S3. Ribosomal proteins that are labeled S7 in 5000 bacteria and S7 or S5 in 3000 eukaryotes hav ...
... only copies of Rps S7 found in multicelllular fungi are not those found in mitochondria. Of the ribosomal proteins of the S subunit that we have studied in detail this uncommon property of S7 is shared by S3. Ribosomal proteins that are labeled S7 in 5000 bacteria and S7 or S5 in 3000 eukaryotes hav ...
Protein-Protein Interactions: Stability, Function and Landscape
... The Accessible surface area (ASA) of the complexes is calculated using an implementaion of the Lee and Richards (1971) algorithm devloped by Hubbard (1992). With a probe sphere, of radius 1.4 angstroms, the ASA was defined as the surface mapped out by the centre of the probe as if it were rolled aro ...
... The Accessible surface area (ASA) of the complexes is calculated using an implementaion of the Lee and Richards (1971) algorithm devloped by Hubbard (1992). With a probe sphere, of radius 1.4 angstroms, the ASA was defined as the surface mapped out by the centre of the probe as if it were rolled aro ...
Machine Learning in the Study of Protein Structure
... different perspectives with different methods • Machine learning is one of the most important tools for understanding genome data • Protein structure prediction is a challenging task given the data we have now ...
... different perspectives with different methods • Machine learning is one of the most important tools for understanding genome data • Protein structure prediction is a challenging task given the data we have now ...
small intestine
... DIGESTION OF FAT The crucial step necessary for fat digestion is emulsification. It is achieved under the influence of bile which is secreted by the liver. Bile contains bile salts, and phospholipid lecithin. The polar part of bile salt and lecitin are soluble in water, the remaining non polar part ...
... DIGESTION OF FAT The crucial step necessary for fat digestion is emulsification. It is achieved under the influence of bile which is secreted by the liver. Bile contains bile salts, and phospholipid lecithin. The polar part of bile salt and lecitin are soluble in water, the remaining non polar part ...
Intracellular localization and tissue
... of the MET25 promoter, was a gift from J. H. Hegemann. The pAK843 (SWF1-EGFP), pAK844 (PFA3-EGFP), pAK845 (PFA4-EGFP), and pAK846 (PFA5-EGFP) plasmids were constructed as follows. The SWF1, PFA3, PFA4, and PFA5 genes were amplified from pAK826, pAK827, pAK828, and pAK829 plasmids, respectively, usin ...
... of the MET25 promoter, was a gift from J. H. Hegemann. The pAK843 (SWF1-EGFP), pAK844 (PFA3-EGFP), pAK845 (PFA4-EGFP), and pAK846 (PFA5-EGFP) plasmids were constructed as follows. The SWF1, PFA3, PFA4, and PFA5 genes were amplified from pAK826, pAK827, pAK828, and pAK829 plasmids, respectively, usin ...
Protein Purification and Analysis Ion exchange
... Protein Purification and Analysis Chromatography Important steps in chromatography 1. Pack column - Column is packed with material (resin) that can absorb molecules based on some property (charge, size, binding affinity, polarity) 2. Equilibrate column - Column is washed with several column volumes ...
... Protein Purification and Analysis Chromatography Important steps in chromatography 1. Pack column - Column is packed with material (resin) that can absorb molecules based on some property (charge, size, binding affinity, polarity) 2. Equilibrate column - Column is washed with several column volumes ...
by Tajekesa KP Blee, Nicola K. Gray, and Matthew
... Modulation of the cytoplasmic functions of mammalian post-transcriptional regulatory proteins by methylation and acetylation: a key layer of regulation waiting to be uncovered? by Tajekesa K.P. Blee, Nicola K. Gray, and Matthew Brook ...
... Modulation of the cytoplasmic functions of mammalian post-transcriptional regulatory proteins by methylation and acetylation: a key layer of regulation waiting to be uncovered? by Tajekesa K.P. Blee, Nicola K. Gray, and Matthew Brook ...
carbohydrate, protein and fat.
... Fats coat the flour particles and prevent them from absorbing water. This reduces the formation of gluten development, which would cause the dough to become elastic. Fats such as pure vegetable fats or lard are suitable for shortening because of their low water content. There are distinctive colours ...
... Fats coat the flour particles and prevent them from absorbing water. This reduces the formation of gluten development, which would cause the dough to become elastic. Fats such as pure vegetable fats or lard are suitable for shortening because of their low water content. There are distinctive colours ...
Proteolysis in Mixed Organic-Aqueous Solvent
... acid databases. Although this procedure has become routine, it is inefficient regarding the time required to prepare and perform gel separation and the percentage of proteins actually visualized. Despite improvements in 2D-gel electrophoresis, very large or very small hydrophobic proteins, as well a ...
... acid databases. Although this procedure has become routine, it is inefficient regarding the time required to prepare and perform gel separation and the percentage of proteins actually visualized. Despite improvements in 2D-gel electrophoresis, very large or very small hydrophobic proteins, as well a ...
LysM, a widely distributed protein motif for binding to
... Medical Microbiology, University Medical Center Groningen, Hanzeplein 1, 9700 RB, Groningen, the Netherlands; ‡Membrane Enzymology Groningen Biomolecular Sciences and Biotechnology institute, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, the Netherlands. §These authors contributed equal ...
... Medical Microbiology, University Medical Center Groningen, Hanzeplein 1, 9700 RB, Groningen, the Netherlands; ‡Membrane Enzymology Groningen Biomolecular Sciences and Biotechnology institute, University of Groningen, Nijenborgh 4, 9747 AG, Groningen, the Netherlands. §These authors contributed equal ...
Department of Health Information Management
... • Family: the proteins in the same family are homologous, evolved from the same ancestor. Usually, the identity of two sequences are very high. • Super Family: distant homologous sequences, evolved from the same ancestor. Sequence identity is around 25%30%. • Fold: only shapes are similar, no homolo ...
... • Family: the proteins in the same family are homologous, evolved from the same ancestor. Usually, the identity of two sequences are very high. • Super Family: distant homologous sequences, evolved from the same ancestor. Sequence identity is around 25%30%. • Fold: only shapes are similar, no homolo ...
Different sorting of nearly similar membrane proteins to the plasma
... K+ channels can be found in the plasma membrane but also in the membrane of organelles such as mitochondria.The mechanism of protein synthesis and the targeting of these similar K+ channel proteins to the different sites of action are mostly unkn ...
... K+ channels can be found in the plasma membrane but also in the membrane of organelles such as mitochondria.The mechanism of protein synthesis and the targeting of these similar K+ channel proteins to the different sites of action are mostly unkn ...
University of North Carolina researchers provide evidence for how
... “We know a lot about LUCA and we are beginning to learn about the chemistry that produced building blocks like amino acids, but between the two there is a desert of knowledge,” Carter said. “We haven’t even known how to explore it.” The UNC research represents an outpost in that desert. “Dr. Wolfend ...
... “We know a lot about LUCA and we are beginning to learn about the chemistry that produced building blocks like amino acids, but between the two there is a desert of knowledge,” Carter said. “We haven’t even known how to explore it.” The UNC research represents an outpost in that desert. “Dr. Wolfend ...
peptides
... Oligopeptide (peptide): a short chain of 20-30 amino acids Polypeptide: a longer peptide with no particular structure Protein: a polypeptide chains with an organized 3D structures The average molecular weight of an amino acid residue is about 110 The molecular weights of most proteins are between 55 ...
... Oligopeptide (peptide): a short chain of 20-30 amino acids Polypeptide: a longer peptide with no particular structure Protein: a polypeptide chains with an organized 3D structures The average molecular weight of an amino acid residue is about 110 The molecular weights of most proteins are between 55 ...
Insilico drug design
... • Rank the ligands according to the interaction energy. • Based on the energy force-field function. ...
... • Rank the ligands according to the interaction energy. • Based on the energy force-field function. ...
Gene Expression, Protein Control
... In absence of inducer, repressor binds to operator In presence of inducer, repressor cannot bind RNA Pol can bind promoter Transcription can occur Negative control when repressor binds Catabolite Repression (other sugars for food) Utilization of sugar (not Glc) controlled by cAMP cAMP CAP Protein ...
... In absence of inducer, repressor binds to operator In presence of inducer, repressor cannot bind RNA Pol can bind promoter Transcription can occur Negative control when repressor binds Catabolite Repression (other sugars for food) Utilization of sugar (not Glc) controlled by cAMP cAMP CAP Protein ...
Endoplasmic Reticulum, Golgi Apparatus, and Lysosomes
... http://www.nature.com/scitable/topicpage/endoplasmic‐reticulum‐golgi‐apparatus‐and‐lysosomes‐ ...
... http://www.nature.com/scitable/topicpage/endoplasmic‐reticulum‐golgi‐apparatus‐and‐lysosomes‐ ...
Hb low affinity for O 2 at low p O 2
... Allosteric Interactions • Allosteric interaction occur when specific molecules bind a protein and modulates activity • Allosteric modulators or allosteric effectors • Bind reversibly to site separate from functional binding or active site • Modulation of activity occurs through change in protein co ...
... Allosteric Interactions • Allosteric interaction occur when specific molecules bind a protein and modulates activity • Allosteric modulators or allosteric effectors • Bind reversibly to site separate from functional binding or active site • Modulation of activity occurs through change in protein co ...
Age-Related Macular Degeneration: On the Rise as Baby Boomers
... multidose, dose escalation study is evaluating treatment with RXI-109 in patients with subretinal scarring associated with AMD. The primary objective is to evaluate the safety, tolerability and pharmacokinetics of single and multiple doses of RXI-109 when administered by intravitreal injection. Seco ...
... multidose, dose escalation study is evaluating treatment with RXI-109 in patients with subretinal scarring associated with AMD. The primary objective is to evaluate the safety, tolerability and pharmacokinetics of single and multiple doses of RXI-109 when administered by intravitreal injection. Seco ...
Important Factors Influencing Protein Solubility for 2-D - Bio-Rad
... proteins in a sample. This may be done to reduce sample complexity, or if solubility conditions for the proteins of interest have already been determined. ReadyPrep™ protein extraction kits operate on the principle of selective inclusion or omission of different solubilizing agents to accomplish sam ...
... proteins in a sample. This may be done to reduce sample complexity, or if solubility conditions for the proteins of interest have already been determined. ReadyPrep™ protein extraction kits operate on the principle of selective inclusion or omission of different solubilizing agents to accomplish sam ...
Presentazione standard di PowerPoint
... Carbohydrates, for example, contain sugar and polysaccharides. Sugars are compounds that can be found in fruit, but also in milk and many other kinds of food. Generally they are easily recognized by their sweet taste. Polysaccharides are not sweet, though they are made of sugars. However, they are t ...
... Carbohydrates, for example, contain sugar and polysaccharides. Sugars are compounds that can be found in fruit, but also in milk and many other kinds of food. Generally they are easily recognized by their sweet taste. Polysaccharides are not sweet, though they are made of sugars. However, they are t ...
Illustration of Skeletal Muscle Calsequestrin Complex Formation by
... immunofluorescence microscopy [2]. Nuclei were identified by blue DAPI staining. The preparation of muscle tissues, the characterization of monoclonal antibodies, the description of materials used and the outline of standard biochemical and cell biological techniques has previously been published [2 ...
... immunofluorescence microscopy [2]. Nuclei were identified by blue DAPI staining. The preparation of muscle tissues, the characterization of monoclonal antibodies, the description of materials used and the outline of standard biochemical and cell biological techniques has previously been published [2 ...
Intrinsically disordered proteins
An intrinsically disordered protein (IDP) is a protein that lacks a fixed or ordered three-dimensional structure. IDPs cover a spectrum of states from fully unstructured to partially structured and include random coils, (pre-)molten globules, and large multi-domain proteins connected by flexible linkers. They constitute one of the main types of protein (alongside globular, fibrous and membrane proteins).The discovery of IDPs has challenged the traditional protein structure paradigm, that protein function depends on a fixed three-dimensional structure. This dogma has been challenged over the last decades by increasing evidence from various branches of structural biology, suggesting that protein dynamics may be highly relevant for such systems. Despite their lack of stable structure, IDPs are a very large and functionally important class of proteins. In some cases, IDPs can adopt a fixed three-dimensional structure after binding to other macromolecules.